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48results about How to "Improve experiment results" patented technology

TaqMan-MGB fluorescent quantitative polymerase chain reaction (PCR) detection primer, TaqMan-MGB fluorescent quantitative PCR detection probe and TaqMan-MGB fluorescent quantitative PCR detection method for seneca valley virus (SVV)

The invention relates to the technical field of molecular biology detection, in particular to a TaqMan-MGB fluorescent quantitative polymerase chain reaction (PCR) detection specific primer (as shown in SEQ ID No. 1 and SEQ ID No. 2), a TaqMan-MGB fluorescent quantitative PCR detection probe as shown in SEQ ID No. 3) and a TaqMan-MGB fluorescent quantitative PCR detection method for seneca valley virus (SVV). The method comprises the steps of drawing a standard curve; extracting ribonucleic acid (RNA) of a sample virus; carrying out reverse transcription on the RNA of the sample virus; enabling the product of the reverse transcription to have a TaqMan-MGB fluorescent quantitative PCR, and reading the result. The primer provided by the invention has better specificity and sensitivity; the MGB probe provided by the invention is shorter and is beneficial to probe design, and the Tm value difference between a paired template and a non-paired template is improved, so that the experimental result is more stable and accurate; the method provided by the invention has the advantages of being simple and rapid, easy to operate, visual in results, high in sensitivity, good in stability, real-time quantitative, and the like, and shortens the reaction time.
Owner:WENS FOOD GRP CO LTD

Pedestrian attribute identification system and method based on multilayer feature learning

The invention discloses a pedestrian attribute recognition system and method based on multi-layer feature learning, the system comprises a feature bottom-to-top extraction module, a bottom-to-top feature fusion module, a feature prediction module, a multi-layer prediction fusion module and a test module, and the method comprises the following specific steps: processing pictures layer by layer frombottom to top to obtain multi-layer features; fusing the features of the adjacent layers layer by layer from top to bottom, compressing the channel by the feature map obtained by the higher layer, carrying out feature fusion and channel dimension reduction on the compressed channel and the feature map sampled by the upper layer, and outputting the feature of the current layer; obtaining preliminary prediction results of different levels through a maximum pooling layer and a full connection layer according to the fused features and the extracted uppermost features; overlapping the preliminaryprediction results of different levels, and correspondingly endowing each attribute predicted by each level with a weight value to obtain a final prediction result; and extracting a prediction resultcorresponding to the picture, and calculating a result of each index. According to the method, a group of specific weights are learned for each attribute according to the predicted values obtained bythe fused features, so that each attribute can better utilize multi-layer features to obtain a better recognition effect.
Owner:SUN YAT SEN UNIV

Apparatus and method for precisely detecting Bauschinger effect of repeated bending of metal plate

The invention relates to an apparatus and method for precisely detecting Bauschinger effect of repeated bending of a metal plate, the apparatus comprises a mechanics loading module and a plate clamping adjustable module fixed at the bottom of the mechanics loading module; the mechanics loading module is fixed and suspended through a testing machine and is vertically arranged, X-direction regulating blocks are symmetrically arranged at left side and right side along X-direction of the mechanics loading module, the front end and rear end of each X-direction regulating block are respectively fixed on the Y-direction regulating blocks, and is capable of respectively being driven by the Y-direction regulating blocks to horizontally slide on a pedestal along the Y-direction back and forth; the operation is easy and has automation function, force and displacement during a loading process can be measured, the coordinate figure, curvature radius and strain value at random point on both surface of the plate during the loading process can be measured, the error of test data is less, and the flexibility requirement of the Bauschinger effect for the plate can be satisfied, so that accurate calibration basis for determining material parameter of Bauschinger effect for the plate can be provided.
Owner:WUHAN UNIV OF TECH

Cervices intraepithelial neoplasia model and model establishing method

InactiveCN101125102ARich sourcesExplore the mechanism of carcinogenesisDiagnosticsSurgeryHuman tumorImmunodeficiency
The present invention relates to a cervical intraepithelial neoplasia model and the method of establishing model, which is characterized in that the cervical intraepithelial neoplasia tissue is embedded in the immunodeficiency mice to establish cervical intraepithelial neoplasia model. The method of establishing model is characterized in that the cervical intraepithelial neoplasia tissue is respectively taken from the biopsy under the vaginoscope and the tissues which are confirmed by the department of pathology and is inoculated subcutaneously in the back of the mice; the immunodeficiency mice of the present invention can overcome the xenoislet immune rejection reaction and receive the transplantation of the human tumor tissues, the tissue model after the transplantation has significant and stable character, short observation period and greater clinical reference significance of the experimental results. The present invention can explore the mechanism of the carcinogenesis of cervical carcinoma by outcome of the pathological process of CIN I, CIN II and CIN III animal models. Furthermore, by observing the period of the outcome of the cervical intraepithelial neoplasia tissue of the animal model, the present invention provides the feasible clinical animal experimental model for reversing the malignant transformation of the CIN I and CIN II lesion tissues.
Owner:ANHUI PROVINCIAL HOSPITAL

Two-degree-of-freedom closed chain flexible mechanism vibration measurement and control device and method

The invention discloses a two-degree-of-freedom closed chain flexible mechanism vibration measurement and control device and method, the device comprises a static platform, a lead screw guide rail, a sliding block is arranged on the lead screw guide rail, the sliding block is connected with a long flexible rod, a flexible protrusion beam is arranged on the extending line of the long flexible rod, a short flexible rod is connected with the above joint through a rotating shaft, the other end of the short flexible rod is connected with a rigid crank, the lead screw guide rail and the rigid crank are connected with an AC servo motor, piezoelectric patches and piezoelectric drivers are pasted to the long and short flexible rods, and the flexible protrusion beam is pasted to an acceleration sensor. According to the invention, vibration information of two flexible connecting rods is acquired and inputted to a computer to analyze and process, and purposes of vibration detection and control are achieved.
Owner:SOUTH CHINA UNIV OF TECH

High-low temperature magnetic field detection system

The invention discloses a high-low temperature magnetic field detection system and relates to a device for detecting magnetic element magnetic field distribution characteristics under high-low temperature environments. The high-low temperature magnetic field detection system comprises a box, a measured workpiece and a Hall probe. The box is made of a nonmagnetic heat insulation material, the Hall probe is installed a heat insulation carrier capable of freely extending in and being drawn out of the box, an infrared temperature detecting instrument for measuring the temperature of the surface of the measured workpiece is arranged outside the box, a distance measuring device for measuring the distance between the Hall probe and the measured workpiece is further arranged on the outer portion of the box, and the measured workpiece performs refrigeration or heating through a liquid nitrogen inlet and a hot nitrogen inlet which are arranged on the box and are symmetrically distributed. The high-low temperature magnetic field detection system is quick in heating or refrigeration efficiency, small in power consumption and suitable for batch production, high-low temperature switching is performed in the box, a temperature fatigue test can be performed, and the high-low temperature magnetic field detection system is simple and convenient and reduces cost; the Hall probe enters the box during the measurement and is drawn out at once after the measurement is finished, and the damage to the Hall probe caused by high temperature is avoided.
Owner:HANGZHOU MINGZHE MAGNETIC TECH

Visualized test bench for testing hydraulic valve performance

The invention relates to a visualized test bench for testing hydraulic valve performance, relating to the field of hydraulic valve performance testing devices, and aiming at solving the problems that the condition of runner flow field of a valve cannot be effectively known, and the experimental valve pressure flow condition is uncontrollable during the experimental process when the test bench in the prior art is used. The visualized test bench for testing hydraulic valve performance comprises a fuel tank, a hydraulic pump No. 1, a pressure gauge, a speed regulating valve, a pilot-type constant pressure reducing valve, a pressure sensor No. 1, a visualized experimental valve, a pressure sensor No. 2, a pilot type relief valve, a flow sensor, a temperature sensor, an electromagnetic reversing valve, a cooler, a hydraulic pump No. 2 and a heater. The visualized test bench is mainly used for the hydraulic valve performance testing process, and an operator can conveniently observe the condition of the runner flow field when the valve is working.
Owner:HARBIN INST OF TECH

Experiment system for simulating fault creep and stick-slip dislocation tunnel structure

The invention belongs to the technical field of rock mass engineering geomechanics, aims to solve the problem of poor reliability of data obtained by an existing test device, and particularly relates to an experiment system for simulating a fault creep and stick-slip dislocation tunnel structure, which comprises a box body structure, a support device and a fault dislocation loading system. A friction effect layer, a first surrounding rock layer, a tunnel structure model, a second surrounding rock layer and an overburden pressure layer are sequentially arranged in the box body structure from bottom to top; a through hole is formed in the bottom of the box body structure, and a plate-shaped assembly comprising a first guide plate, a second guide plate and a loading plate is arranged at the upper part of the through hole; a first slide rail and a second slide rail are respectively arranged on the inner sides of the first guide plate and the second guide plate; the loading plate moves along the first sliding rail and the second sliding rail under the action of the fault movement loading system, stick-slip and creep movement effects required by a test are generated through the friction effect layer, and fault movement force is transmitted to the tunnel structure model through the first surrounding rock layer. According to the invention, reliable and effective simulation test data can be obtained.
Owner:INST OF GEOLOGY & GEOPHYSICS CHINESE ACAD OF SCI

Water culture planting frame based on wheat culture

The invention discloses a water culture planting frame based on wheat culture and relates to the technical field of wheat culture. The water culture planting frame disclosed by the invention comprisesa frame body and a culture device and also comprises a water pumping mechanism and a water inlet assembly, wherein the frame body comprises five support frames, the cross section of each support frame is of a right-angle trapezoidal structure, four groups of transverse rods are fixedly arranged on the five support frames in a penetrated mode, and the four groups of transverse rods are linearly arranged obliquely. The water pumping mechanism is used for pumping a nutrient solution inside each water culture box into a charging box, a water pumping pipeline is used for discharging the nutrient solution inside the charging box into each water culture box to form a circulation of the nutrient solution, simultaneously, an EC sensor inside the charging box is utilized for detecting the nutrientconcentration of the nutrient solution, and a control device is utilized for controlling a third electric flow valve on a discharging pipe of a liquid storing box to be opened and closed, thereby solving the problem that an existing water culture planting frame for wheat culture does not have a function of automatically adding the nutrient solution so that manpower and material resources are excessively wasted.
Owner:LIANYUNGANG ACAD OF AGRI SCI

Method for rapidly detecting and quantifying activity of proteinase in dry-cured ham processing process

InactiveCN106701893ALow costReduce labor intensityMicrobiological testing/measurementProtein Degradation ProcessCathepsin S activity
The invention relates to a method for rapidly detecting and quantifying the activity of proteinase in a dry-cured ham processing process. The method comprises the following six steps: manufacturing a gelatin smear, collecting and diluting a sample, eluting the proteinase, collecting the proteinase, determining the activity of cathepsin, and observing through a microscope and determining the activity of the cathepsin. According to the method provided by the invention, the proteinase is calibrated and quantified by adopting a green fluorescent proteinase dyeing method, the activity of the proteinase in the dry-cured ham processing process can be rapidly monitored and quantified, and a complicated protein degradation process in the ham processing process is monitored; salinity, water activity, temperature and humidity conditions in the processing process can be adjusted in time according to the activity of the proteinase, so as to realize quality regulation and control of dry-cured hams; a production and processing process of the dry-cured hams is improved and the curing of the dry-cured hams is accelerated, so that the method provided by the invention has important practical significance on facilitation of industrial production of the dry-cured hams in China.
Owner:夏云

Device for interactive reaction between heavy metals and particulate matter and reaction method

The invention discloses a device for interactive reaction between heavy metals and particulate matter and belongs to the field of interactive reaction devices. The device comprises a flue gas mixing system, a temperature control hearth and a tail gas processing system, wherein a temperature control device is arranged in the temperature control hearth, an ejector, an atomizer and a sampling hole are respectively arranged outside of the temperature control hearth, the front end of the ejector is connected with one end of the flue gas mixing system and one end of the atomizer respectively, the other end of the atomizer is connected with a peristaltic pump, and the tail end of the temperature control hearth is connected with the tail gas processing system. The device can be used for researching a reaction mechanism of specific ingredients of particulate matter and heavy metals under different conditions in different concentrations of heavy metal atmospheres, and the peristaltic pump combines with an atomizer and double-by-path venturi ejector combined device, so that the particulate matter and the heavy metal atmospheres re evenly mixed, and control is convenient. In addition, feeding is performed from the cylindrical tangent plane of the hearth, so that mixing is violent, and the staying time of fine particulate matter and heavy metal flue gas in the hearth is also prolonged.
Owner:SHENYANG AEROSPACE UNIVERSITY

Image generation method based on discrete Fourier transform attention mechanism

The invention discloses an image generation method based on a Fourier transform attention mechanism, and belongs to the field of computer vision. The method comprises the following steps: firstly, selecting a generative adversarial network as a basic framework, normalizing training pictures, and sampling normal distribution to obtain noise; improving the original attention mechanism, using the characteristic that discrete Fourier transform can be combined with all position information to carry out calculation for replacing an original calculation pixel point correlation feature map part with high complexity; and obtaining a feature map combining all position information. During training, noise and picture categories are input into the network at the same time, and a generative adversarial network algorithm is utilized to train the model. After the network is trained, the image generation task can be completed by inputting noise and picture categories in the generative adversarial. According to the invention, the calculation complexity and the time complexity of a self-attention mechanism can be remarkably reduced, and the quality of images generated by an existing method and the diversity of the images are improved.
Owner:YANGTZE DELTA REGION INST (QUZHOU) UNIV OF ELECTRONIC SCI & TECH OF CHINA +1

Device and method for precise detection of Bauschinger effect in repeated bending of sheet metal

The invention relates to an apparatus and method for precisely detecting Bauschinger effect of repeated bending of a metal plate, the apparatus comprises a mechanics loading module and a plate clamping adjustable module fixed at the bottom of the mechanics loading module; the mechanics loading module is fixed and suspended through a testing machine and is vertically arranged, X-direction regulating blocks are symmetrically arranged at left side and right side along X-direction of the mechanics loading module, the front end and rear end of each X-direction regulating block are respectively fixed on the Y-direction regulating blocks, and is capable of respectively being driven by the Y-direction regulating blocks to horizontally slide on a pedestal along the Y-direction back and forth; the operation is easy and has automation function, force and displacement during a loading process can be measured, the coordinate figure, curvature radius and strain value at random point on both surface of the plate during the loading process can be measured, the error of test data is less, and the flexibility requirement of the Bauschinger effect for the plate can be satisfied, so that accurate calibration basis for determining material parameter of Bauschinger effect for the plate can be provided.
Owner:WUHAN UNIV OF TECH

Nested easily adjusted cylindrical lithium battery thermal runaway experimental platform

The invention discloses a nested easily adjusted cylindrical lithium battery thermal runaway experimental platform. An upper iron pipe and a lower iron pipe are nested and are fixed on an iron stand after being clamped by a clamp; a thermocouple storage slideway is arranged on the inner wall of the lower iron pipe; a thermocouple is stored in the thermocouple storage slideway; one side of the thermocouple is provided with a cylindrical lithium ion battery and a heating stick which are closely fitted; a heating stick hole and a size-adjustable thermocouple hole are formed in the bottom of the lower iron pipe; the thermocouple hole is drivingly connected with a knob; the thermocouple is connected with an NI upper data acquirer; the upper iron pipe is connected with a flue gas analyzer; and aviewing window is further formed in the upper iron pipe. The nested easily adjusted cylindrical lithium battery thermal runaway experimental platform decreases the number of operating steps and improves the experimental efficiency; meanwhile, the problems of a flue gas collection error and the like caused by a conventional flue gas hood are solved; and gas is directly collected in a test cabin, so that an experimental result is more accurate.
Owner:CIVIL AVIATION FLIGHT UNIV OF CHINA

Instrument for sectioning and dyeing biological sample on electronic microscope

InactiveCN102680303BReduce manual repetitive tedious processesReduce generationPreparing sample for investigationBiochemical engineeringElectron microscope
The invention relates to an instrument for sectioning and dyeing a biological sample on an electronic microscope, which belongs to the field of biological sample preparation. The instrument is composed of a locating handle, a water outlet, a groove flap board, a wattle, a built-in coverless box, a top cover, a stable gasket and a groove frame, wherein the groove flap board is arranged in a top groove of the groove frame; the locating handle is arranged on the outer side of the groove frame; the wattle is arranged above the groove flap board; the built-in coverless box is located in the top groove of the groove frame and arranged on one side of the groove flap board; the top cover is covered on the top groove of the groove frame; and the bottom part of the groove frame is provided with the stable gasket. According to the invention, the instrument can perform dyeing on biological sample parity for many times without repeated manual operations; thus, the time difference can be reduced; the generation of pollutant is eliminated; a high-quality experiment result is ensured to obtain; and the instrument can be used for immunohistochemical incubation of a histochemical microscope and preparation of macromolecule sample drop tablets and the like.
Owner:FUDAN UNIV

Pedestrian attribute recognition system and method based on multi-layer feature learning

The invention discloses a pedestrian attribute recognition system and method based on multi-layer feature learning. The system includes a bottom-up feature extraction module, a top-down feature fusion module, a feature prediction module, a multi-layer prediction fusion module and a test module. , the specific steps of this method are: process the image layer by layer from bottom to top to obtain multi-layer features; fuse the features of adjacent layers from top to bottom, compress the channel of the feature map obtained by the higher layer, and combine it with the upper layer The sampled feature map is subjected to feature fusion and channel dimensionality reduction, and the current layer features are output; the fused features and the extracted uppermost layer features are passed through the maximum pooling layer and the fully connected layer to obtain preliminary prediction results at different levels; The preliminary prediction results are superimposed, and each attribute of each layer of prediction is given a corresponding weight value to obtain the final prediction result; the prediction result corresponding to the picture is extracted, and the results of each index are calculated. The present invention learns a set of specific weights for each attribute based on the predicted value obtained by the fused features, so that each attribute can better use multi-layer features to obtain better recognition effect.
Owner:SUN YAT SEN UNIV

Antithrombin III determination kit

InactiveCN111826418AAct as a quality improverImprove assay reaction rateMicrobiological testing/measurementBiological material analysisAntithrombin assayChromogenic Substrates
The invention relates to the technical field of in-vitro diagnosis, and particularly relates to an antithrombin III determination kit, and overcomes the defects that the determination rate of a determination kit in the prior art is low, and after determination is completed, a reagent reaction is still carried out continuously, so that the numerical value of a determination experiment result is influenced, and a final result is deviated. The antithrombin III determination kit comprises a reagent I, a reagent II, a speed-up solution and a stop solution, wherein the reagent I comprises thrombin,heparin, a buffer solution and salt; the reagent II comprises a chromogenic substrate of the thrombin, a buffer solution and mannitol; and the speed-up solution comprises a disodium hydrogen phosphatesolution; the stop solution comprises polyvinyl chloride resin and sodium azide. The reagent I, the reagent II, the speed-up solution and the stop solution are combined to form the kit, the antithrombin III is determined quickly by utilizing the reagent I, the reagent II and the speed-up solution, and the reaction is stopped by utilizing the stop solution after determination is completed, so thatdata deviation caused by slow and continuous reaction is avoided; the whole determination kit is convenient to use, quick in determination and accurate in data.
Owner:BAODING TIANYUE BIOENG
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