Human immunity function restructuring mouse endometriosis model and model building method

Abstract

The human immunity function reconstructed mouse endometriosiss model and its constructing method features that human early pregnancy peripheral blood mononuclear cell is fed into non-obese diabetic serious combined immune deficiency mouse body to reconstruct immunity function and the decidual tissue of the same human is planted in the mouse body to establish human immunity function reconstructed mouse endometriosiss model. The model establishing method features that the peripheral blood and the decidual tissue are taken from the identical woman in artificial abortion operation. The peripheral blood mononuclear cell is fed into mouse body, the decidual tissue is planted under skin of mouse abdomen, and the cut is sawn with bacteria-free suture. The NOD-SCID mouse has human immune, transplanted tissue maintaining the tissue state of endometrium, obvious and stable characteristic, short observation period and experiment result with great clinical reference significance.

Description

Technical field: [0001] The invention relates to a model of endometriosis and a method for establishing the model Background technique: [0002] EM is a common benign gynecological disease that mostly occurs in women of childbearing age; in recent years, its incidence has been on the rise. So far, the pathogenesis of endometriosis is still unclear, and laparoscopy or surgical pathology is needed Histological examination confirmed the diagnosis, and the drug treatment and postoperative recurrence rate were high. [0003] EM models can be established by transplantation of syngeneic in vivo membranes in baboons, rabbits, macaques, and rats, but due to the large differences in phylogenetic and biochemical characteristics of the endometrium of these animals and humans, there are many limitations of such models. limited. [0004] NOD-SCID mice are non-obese diabetic severe combined immunodeficiency mice, which are animals with congenital T and B lymphocyte combined immunodeficie...

AI Technical Summary

Problems solved by technology

[0004] NOD-SCID mice are non-obese diabetic severe combined immunodeficiency mice, which are animals with congenital T and B lymphocyte combined immunodeficiency, which can overcome the immune rejection between different species, and accept human normal and tumor tissue transplantation. The tissue still maintains the original tissue morphology and biochemical characteristics, which is closer to the pathological changes of human diseases; however, due to the immunodeficiency of NOD-SCID mice, immune regulation cannot be performed, and the secretory endometrium of normal people is not easy to obtain

[0005] The existence of the above reasons greatly limits the research on this disease

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