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Cervices intraepithelial neoplasia model and model establishing method

A method for establishing a cervix, a technology for cervix, applied in medical science, surgery, diagnosis, etc., can solve the problem of not establishing an animal model, and achieve the effects of controllable microorganisms in vivo, clear genetic background, and high success rate of establishment

Inactive Publication Date: 2008-02-20
ANHUI PROVINCIAL HOSPITAL
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However, so far, there is no report on the establishment of related animal models

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  • Cervices intraepithelial neoplasia model and model establishing method

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[0031] The cervical intraepithelial neoplasia model in this example is to implant cervical intraepithelial neoplasia tissues, including CINI, CINII, and CINIII, into non-obese diabetic severe combined immunodeficiency NOD-SCID mice respectively to establish cervical epithelial neoplasia. Intraneoplasia model.

[0032] The establishment of the model is carried out at room temperature according to the following steps:

[0033] Materials:

[0034] Cervical intraepithelial neoplasia (CINI, CINII, CINIII) tissues were taken from colposcopic biopsies and pathologically confirmed tissues, and the taken tissues were placed in 5 ml sterile bottles of RPMI1640 culture medium, and then placed in ice boxes; specific implementation Among them, PMRI1640 culture medium can contain 100U / ml penicillin and 100U / ml streptomycin to prevent infection in mice.

[0035] 1. Animals:

[0036] Take NOD-SCID, 6-8 weeks old, sexually mature, female.

[0037] 2. Treatment before inoculation:

[0038]...

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Abstract

The present invention relates to a cervical intraepithelial neoplasia model and the method of establishing model, which is characterized in that the cervical intraepithelial neoplasia tissue is embedded in the immunodeficiency mice to establish cervical intraepithelial neoplasia model. The method of establishing model is characterized in that the cervical intraepithelial neoplasia tissue is respectively taken from the biopsy under the vaginoscope and the tissues which are confirmed by the department of pathology and is inoculated subcutaneously in the back of the mice; the immunodeficiency mice of the present invention can overcome the xenoislet immune rejection reaction and receive the transplantation of the human tumor tissues, the tissue model after the transplantation has significant and stable character, short observation period and greater clinical reference significance of the experimental results. The present invention can explore the mechanism of the carcinogenesis of cervical carcinoma by outcome of the pathological process of CIN I, CIN II and CIN III animal models. Furthermore, by observing the period of the outcome of the cervical intraepithelial neoplasia tissue of the animal model, the present invention provides the feasible clinical animal experimental model for reversing the malignant transformation of the CIN I and CIN II lesion tissues.

Description

technical field [0001] The invention relates to a cervical intraepithelial neoplasia model and a method for establishing the model. Background technique [0002] Cervical cancer is one of the most common tumors in the human body. It not only occupies the first place in female reproductive system tumors, but also is the most common tumor among various malignant tumors in women. The mortality rate of cervical cancer in my country ranks fourth among all cancers and ranks second among female cancers. [0003] Cervical intraepithelial neoplasia (CIN) is a group of precancerous lesions related to cervical invasive carcinoma, which can reflect the continuous process of cervical cancer development. The pathological features of CIN are neoplasia limited to the squamous epithelium of the cervix, including abnormal cell arrangement and nuclear abnormalities. According to their different degrees, atypical hyperplasia can be divided into CINI grade, CINII grade and CINIII grade, which ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B19/00A61B90/00
Inventor 凌斌周颖冯定庆沈国栋赵卫东李敏高宗侠朱园园
Owner ANHUI PROVINCIAL HOSPITAL
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