Composition for promoting wound healing of pets as well as preparation method and application of composition

Abstract

The invention discloses a composition for promoting wound healing of pets and a preparation method and application of the composition. A nutritional supplement comprises chlorogenic acid and glutamine. The nutritional supplement is wide in raw material source, healthy, rich in nutrition, good in palatability, low in price and easy to obtain; by using the specific formula, especially the chlorogenic acid and glutamine in a specific ratio, nutrient substances required by growth and development of pets can be supplemented, postoperative wound healing of the pets can be effectively promoted, postoperative pain, discomfort and inflammation of the pets can be reduced, immunity of the pets can be improved, no antibiotic component is added into the supplement, and the supplement is free of toxic and side effects. The liver and kidney burden of pets can be relieved, and the risk that bacteria generate drug resistance is avoided; the preparation method of the nutritional supplement only needs processing technologies such as crushing, sieving, homogenizing and emulsifying, the operation process is simple and easy to learn, and industrial large-scale production is facilitated.

Description

Technical Field

[0001] The invention relates to the field of nutritional supplements, and in particular to a composition for promoting wound healing of pets, and a preparation method and application thereof. Background Art

[0002] With the accelerated pace of life and the improvement of living standards, more and more people choose to keep pets to meet their spiritual needs. Sterilization of pets can not only effectively control the number of pets and avoid the increase of stray animals, but also prevent some diseases related to the reproductive system. Post-sterilization care and rehabilitation are issues of great concern to pet owners, because postoperative recovery directly affects the health and quality of life of pets. Slow wound healing may cause anxiety to pet owners. After sterilization, pets may also experience loss of appetite and decreased activity.

[0003] CN117322549A discloses a pet staple food for enhancing puppies' immunity and promoting wound healing, which comprises the following raw materials by weight: 50-70 parts of basic dog food, 0.25-0.35 parts of chitosan oligosaccharide, 0.14-0.16 parts of red fructo-oligosaccharide and 0.12-0.13 parts of prebiotics. However, dry food is generally hard feed, and pets need to chew it to digest and absorb it well. Pets in the recovery period have poor appetite and are unwilling to do too much chewing. Small food intake will reduce the pet's nutrient absorption and delay the recovery of the wound.

[0004] CN117918458A discloses a nutritional supplement for promoting pet wound recovery and a preparation method thereof, wherein the formula includes ingredients such as Panax notoginseng powder, coenzyme Q10, oligofructose, saccharoterpenoids, air-dried powder and anhydrous glucose. However, the ingredients such as Panax notoginseng powder contained in the formula result in poor taste and mouthfeel of the nutritional supplement, poor palatability, and failure to promote the feeding of pets, especially pets in the recovery period of trauma. Summary of the invention

[0005] In order to overcome the deficiencies in the prior art, the present invention provides a composition for promoting pet wound healing, and a preparation method and application thereof.

[0006] In a first aspect, the present invention provides a composition for promoting wound healing of pets.

[0007] Furthermore, the composition comprises chlorogenic acid and glutamine.

[0008] Furthermore, the mass proportion of chlorogenic acid in the composition is 0.2-1.5% (such as 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%).

[0009] Furthermore, the mass percentage of glutamine in the composition is 0.5-2.5% (such as 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%).

[0010] In some embodiments of the present invention, the mass proportion of the chlorogenic acid in the composition is 0.5%, and the mass proportion of the glutamine in the composition is 1%.

[0011] In some embodiments of the present invention, the mass proportion of the chlorogenic acid in the composition is 1%, and the mass proportion of the glutamine in the composition is 2%.

[0012] Furthermore, the composition also includes basic raw materials.

[0013] Furthermore, the composition also includes additives.

[0014] Furthermore, the mass proportion of the basic raw material in the composition is 93.5-97.5% (such as 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%).

[0015] Furthermore, the mass percentage of the additive in the composition is 1-5% (1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%).

[0016] Furthermore, the chlorogenic acid can also be derived from an extract of Eucommia ulmoides powder, and the extraction method thereof comprises the following steps: S1. Mix the Eucommia ulmoides powder and the extractant at a solid-liquid ratio of 0.5 g -1.5 g:10 L, and perform reflux extraction; S2 vacuum concentrating the extract obtained after reflux extraction in step S1; S3 performing gradient elution on the concentrated extract obtained in step S2 to collect different components; S4 using HPLC to detect the components collected in step S3, and determining and collecting the component with the highest chlorogenic acid content; S5: freeze-drying the fraction with the highest chlorogenic acid content collected in step S4 to obtain dry chlorogenic acid powder.

[0017] Furthermore, the concentration of the extractant is 60%-80% (such as 60%, 65%, 70%, 75%, 80%), preferably 70%.

[0018] Furthermore, the extractant is an ethanol-water solution.

[0019] Furthermore, the temperature of the reflux extraction is 50-70°C (such as 50°C, 55°C, 60°C, 65°C, 70°C), preferably 60°C; the time of the reflux extraction is 1-3h (such as 1h, 1.5h, 2h, 2.5h, 3h), preferably 2.5h.

[0020] In some embodiments of the present invention, the solid-liquid ratio is 1:10 (g / L).

[0021] Furthermore, the temperature of the vacuum concentration is 30-50°C (such as 30°C, 35°C, 40°C, 45°C, 50°C), preferably 40°C.

[0022] Furthermore, the conditions of the gradient elution are: using a C18 chromatographic column, the starting solvent is water, and then gradually increasing the proportion of ethanol until the final mobile phase is pure ethanol, the flow rate is 1-2 ml / min, and the column temperature is 35°C.

[0023] Furthermore, before step S1, the method further includes the step of pre-treating the eucommia leaves: washing, drying, and crushing the eucommia leaves into coarse powder, coarsely screening the coarse powder, and then ultra-finely crushing the powder to obtain fine powder.

[0024] In some embodiments of the present invention, before step S1, the step of pre-treating the Eucommia leaves is also included: the Eucommia leaves are washed, dried, and crushed into coarse powder, the coarse powder is sieved through a 40-mesh sieve to remove impurities and Platycodon grandiflorum, and then the coarse powder after the coarse sieve is ultrafinely ground to obtain micron fine powder, the ultrafine grinding time is 20 minutes, and the particle size of the micron fine powder is 1-10μm.

[0025] Furthermore, the basic raw materials include the following components in parts by weight: 100-200 parts of maltose (such as 100 parts, 110 parts, 120 parts, 130 parts, 140 parts, 150 parts, 160 parts, 170 parts, 180 parts, 190 parts, 200 parts), 150-250 parts of chicken (such as 150 parts, 160 parts, 170 parts, 180 parts, 190 parts, 200 parts, 210 parts, 220 parts, 230 parts, 240 parts, 250 parts), 30-70 parts of carrots (30 parts, 40 parts, 50 parts 30-70 parts (30, 40, 50, 60, 70 parts), fish oil 30-70 parts (30, 40, 50, 60, 70 parts), glucose 30-70 parts (30, 40, 50, 60, 70 parts), whole milk powder 30-70 parts (30, 40, 50, 60, 70 parts), preferably 320-380 parts of water, 120-180 parts of maltose, 180-220 parts of chicken, 40-60 parts of carrots, 40-60 parts of fish oil, 40-60 parts of glucose, and 40-60 parts of whole milk powder.

[0026] In some embodiments of the present invention, the basic raw materials include the following components in parts by weight: 350 parts of water, 150 parts of maltose, 200 parts of chicken, 50 parts of carrots, 50 parts of fish oil, 50 parts of glucose, and 50 parts of whole milk powder.

[0027] Further, the additive includes vitamin A 100000-200000 IU / kg (such as 100000 IU / kg, 110000 IU / kg, 120000 IU / kg, 130000 IU / kg, 140000 IU / kg, 145000 IU / kg, 146000 IU / kg, 147000 IU / kg, 148000 IU / kg, 149000 IU / kg, 150000 IU / kg, 151000 IU / kg, 152000 IU / kg, 153000 IU / kg, 154000 IU / kg, 155000 IU / kg, 156000 IU / kg, 157000 IU / kg, 158000 IU / kg, 159000 IU / kg, 160000IU / kg, 170000IU / kg, 180000IU / kg, 190000IU / kg, 200000IU / kg), vitamin B1 300-400 mg / kg (such as 300mg / kg, 310mg / kg, 320mg / kg, 330mg / kg, 340mg / kg, 350mg / kg, 360mg / kg, 365mg / kg, 366mg / kg, 367mg / kg, 368mg / kg, 369mg / kg, 370mg / kg, 371mg / kg, 372mg / kg, 373mg / kg, 374mg / kg, 375mg / kg, 380mg / kg, 390mg / kg, 400mg / kg), vitamin B2 10-50 mg / kg (e.g., 10 mg / kg, 20 mg / kg, 30 mg / kg, 40 mg / kg, 50 mg / kg), vitamin B6100-200mg / kg (such as 100mg / kg, 110mg / kg, 120mg / kg, 130mg / kg, 140mg / kg, 145mg / kg, 146mg / kg, 147mg / kg, 148mg / kg, 149m g / kg, 150mg / kg, 151mg / kg, 152mg / kg, 153mg / kg, 154mg / kg, 155mg / kg, 156mg / kg, 157mg / kg, 158mg / kg, 159mg / kg, 160mg / kg, 170mg / kg, 180mg / kg, 190mg / kg, 200mg / kg), vitamin D3 8000-10000IU / kg (such as 8000IU / kg, 8500IU / kg, 8600IU / kg, 8700IU / kg, 8800IU / kg, 8900IU / kg, 9000mg / kg, 9100mg / kg, 9200mg / kg, 9300mg / kg, 9400mg / kg, 9500mg / kg, 10000mg / kg), vitamin E 1000-1600 IU / kg (such as 1000IU / kg, 1100IU / kg, 1200IU / kg, 1250IU / kg, 1300IU / kg, 1350IU / kg, 1400IU / kg, 1500IU / kg, 1600IU / kg), niacin 10-90mg / kg (such as 10mg / kg, 20mg / kg, 30mg / kg, 40mg / kg, 45mg / kg, 50 mg / kg, 55mg / kg, 60mg / kg, 70mg / kg, 80mg / kg, 90mg / kg), folic acid 10-90mg / kg (such as 10mg / kg, 15mg / kg, 20mg / kg, 25mg / kg, 30mg / kg, 40mg / kg, 50mg / kg, 60mg / kg, 70mg / kg, 80mg / kg, 90mg / kg), D-calcium pantothenate10-90mg / kg (such as 10mg / kg, 20mg / kg, 25mg / kg, 30mg / kg, 35mg / kg, 40mg / kg, 50mg / kg, 60mg / kg, 70mg / kg, 80mg / kg, 90mg / kg), potassium iodide 10-90mg / kg (such as 10mg / kg, 15mg / kg, 20mg / kg, 25mg / kg, 30mg / kg, 35mg / kg, 40mg / kg, 50mg / kg, 60mg / kg, 70mg / kg, 80mg / kg, 90mg / kg), manganese sulfate 10 -90mg / kg (such as 10mg / kg, 15mg / kg, 20mg / kg, 25mg / kg, 30mg / kg, 35mg / kg, 40mg / kg, 50mg / kg, 60mg / kg, 70mg / kg, 80mg / kg, 90mg / kg), magnesium sulfate 0.005-0.1% (such as 0.005%, 0.006%, 0.007%, 0.008%, 0.009%, 0.01%, 0.011%, 0.012%, 0.013%, 0.014%, 0.015%, 0.05%, 0.1%), copper sulfate 10-50 mg / kg (such as 10mg / kg, 15mg / kg, 20mg / kg, 25mg / kg, 30mg / kg, 35mg / kg, 40mg / kg, 50mg / kg, 60mg / kg, 70mg / kg, 80mg / kg, 90mg / kg), ferrous lactate 10-90mg / kg (such as 10mg / kg, 20mg / kg, 30mg / kg, 35mg / kg, 40mg / kg, 45mg / kg, 50mg / kg, 55mg / kg, 60mg / kg, 70mg / kg, 80mg / kg, 90mg / kg), sodium carboxymethyl cellulose 0.5-1.5% (such as 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%), preferably, the additives include vitamin A 120000-180000 IU / kg, vitamin B1 310-390 mg / kg, vitamin B2 20-40mg / kg, vitamin B6 120-180mg / kg, vitamin D3 8500-9500 IU / kg, vitamin E 1100-1500 IU / kg, niacin 30-70mg / kg, folic acid 10-50mg / kg, D-pantothenate calcium 10-50mg / kg, potassium iodide 10-50mg / kg, manganese sulfate 10-50mg / kg, magnesium sulfate 0.005-0.05%, copper sulfate 20-40 mg / kg, ferrous lactate 30-70 mg / kg, sodium carboxymethyl cellulose 0.8-1.2%.

[0028] In some embodiments of the present invention, the additives include vitamin A 156000 IU / kg, vitamin B1 372.3 mg / kg, vitamin B2 30 mg / kg, vitamin B6 156 mg / kg, vitamin D3 9000 IU / kg, vitamin E 1300 IU / kg, niacin 50 mg / kg, folic acid 20.6 mg / kg, D-calcium pantothenate 31 mg / kg, potassium iodide 26 mg / kg, manganese sulfate 21 mg / kg, magnesium sulfate 0.01%, copper sulfate 36 mg / kg, ferrous lactate 50 mg / kg, and sodium carboxymethyl cellulose 1%.

[0029] Among them, the mass proportions of various ingredients in the aforementioned additives such as vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3, vitamin E, niacin, folic acid, D-calcium pantothenate, potassium iodide, manganese sulfate, magnesium sulfate, copper sulfate, ferrous lactate, and sodium carboxymethyl cellulose refer to their mass proportions in 1 kg of the composition.

[0030] The second aspect of the present invention provides a method for preparing the composition according to the first aspect, the preparation method comprising the following steps: S1. Cook the chicken and wash the carrots in the basic raw materials, grind them, and sieve them. Then, mix the sieved chicken and carrots with other basic raw materials, and perform homogenization and emulsification treatment to obtain a first emulsified material. S2: adding additives to the first emulsified material, performing homogenization and emulsification treatment, and obtaining a second emulsified material; S3: adding glutamine and chlorogenic acid to the second emulsified material, performing homogenization and emulsification treatment, and obtaining a composition.

[0031] Furthermore, the homogenization and emulsification temperature in step S1 is 70-80°C (such as 70°C, 71°C, 72°C, 73°C, 74°C, 75°C, 76°C, 77°C, 78°C, 79°C, 80°C), preferably 75°C.

[0032] Furthermore, the homogenization and emulsification time in step S1 is 35-45 min (such as 35 min, 36 min, 37 min, 38 min, 39 min, 40 min, 41 min, 42 min, 43 min, 44 min, 45 min), preferably 40 min.

[0033] Furthermore, the homogenization emulsification temperature in step S2 is 70-80°C (such as 70°C, 71°C, 72°C, 73°C, 74°C, 75°C, 76°C, 77°C, 78°C, 79°C, 80°C), preferably 75°C.

[0034] Furthermore, the homogenization and emulsification time in step S2 is 15-25 min (such as 15 min, 16 min, 17 min, 18 min, 19 min, 20 min, 21 min, 22 min, 23 min, 24 min, 25 min), preferably 20 min.

[0035] Furthermore, the homogenization emulsification temperature in step S3 is 55-65°C (such as 55°C, 56°C, 57°C, 58°C, 59°C, 60°C, 61°C, 62°C, 63°C, 64°C, 65°C), preferably 60°C.

[0036] Furthermore, the homogenization and emulsification time in step S3 is 15-25 min (such as 15 min, 16 min, 17 min, 18 min, 19 min, 20 min, 21 min, 22 min, 23 min, 24 min, 25 min), preferably 20 min.

[0037] The third aspect of the present invention provides a use of the nutritional supplement as described in the first aspect or the composition prepared by the preparation method as described in the second aspect in the preparation of a product for promoting pet recovery.

[0038] Furthermore, the pet is a cat, a dog, a mink, a hamster, a rabbit or a meerkat.

[0039] Furthermore, the product is pet food, medicine or toiletries.

[0040] In some embodiments of the present invention, the product is a nutritional paste.

[0041] Furthermore, the promoting pet recovery includes: (1) Reduce postoperative weight loss; (2) Reduce postoperative wound pain; (3) Reduce inflammation of skin wounds; (4) Reduce the swelling of the skin wound; (5) Reduce wound diameter; (6) Improve inflammation in the body; (7) Promote wound healing.

[0042] Beneficial effects:

[0043] The raw materials of the nutritional supplement of the present invention are widely available and healthy, rich in nutrition and palatable, cheap and easily available; the use of the specific formula of the present invention, especially the specific ratio of chlorogenic acid and glutamine, can exert a synergistic effect, and while supplementing the nutrients required for the growth and development of pets, it can effectively promote the wound healing of pets after surgery, reduce the pain and inflammation of pets after surgery, and improve the immunity of pets; the supplement does not add any antibiotic ingredients, which can reduce the burden on the liver and kidneys of pets and avoid the risk of bacteria developing drug resistance; the preparation method of the nutritional supplement of the present invention only requires processing technologies such as crushing, screening, homogenization and emulsification, and the operation process is simple and easy to learn, and it is easy to industrially scale up production. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 Figure 1 shows the changes in weight loss and weight gain of each group of cats after sterilization. Figure A shows the weight loss of each group on the 7th day after sterilization; Figure B shows the weight gain of each group on the 14th day after sterilization; Figure C shows the weight gain of each group on the 21st day after sterilization; different lowercase letters in each group indicate statistically significant differences between the groups ( P <0.05).

[0045] Figure 2 The postoperative wound pain index scores of each group after cat sterilization (different lowercase letters in each group indicate statistically significant differences between the groups) P < 0.05)).

[0046] Figure 3 Scoring of wound inflammation and swelling after sterilization of cats (different lowercase letters in each group indicate statistically significant differences between groups ( P < 0.05)).

[0047] Figure 4 These are frontal photos of the wounds of cats at different days after sterilization. Among them, Figure A is a frontal photo of the wound on the 0th day after sterilization; Figure B is a frontal photo of the wound on the 7th day after sterilization; Figure C is a frontal photo of the wound on the 14th day after sterilization; Figure D is a frontal photo of the wound on the 21st day after sterilization.

[0048] Figure 5 Figure 1 shows the changes in wound diameter at different days after cat sterilization. Figure A shows the changes in wound diameter at different days after female cat sterilization; Figure B shows the changes in wound diameter at different days after male cat sterilization (Compared with the control group in Example 1, *: significant difference ( P <0.05), **: extremely significant difference ( P <0.01), ***: extremely significant difference ( P <0.001)).

[0049] Figure 6 The figures show the changes of serum wound healing related indicators in each group at different days after cat sterilization. Figure A shows the changes of serum epidermal growth factor (EGF) in the blank control group and the cat serum in Example 1; Figure B shows the changes of transforming growth factor-β1 (TGF-β1) in the blank control group and the cat serum in Example 1; Figure C shows the changes of fibronectin (FN) in the blank control group and the cat serum in Example 1. DETAILED DESCRIPTION

[0050] In order to more clearly understand the technical content of the present invention, the following embodiments are given in detail, the purpose of which is only to better understand the content of the present invention but not to limit the protection scope of the present invention.

[0051] The experimental design is as follows: (1) Experimental animals: 42 adult male cats and 42 female cats that required sterilization surgery were selected as experimental subjects. They were ensured to be at similar growth and development stages, including weight and mental state, and the estrus period was avoided. The same sterilization surgery was performed on female cats (vertical incision was performed on female cats, the entire uterus was removed, and the surgical incision was sutured; for male cats, an incision was made in the scrotum to remove the testicles, and the surgical incision was glued with a liquid bandage).

[0052] (2) Experimental grouping: All sterilized cats were randomly divided into seven groups, and each group contained 6 male cats and 6 female cats, namely, blank control group, Example 1, Example 2, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, and it was ensured that there were no significant differences between the cats in each group. Among them, the blank control group was fed with blank nutrition without adding glutamine and chlorogenic acid every day after surgery; Example 1 was tested to feed a nutritional paste with 1% glutamine and 0.5% extracted chlorogenic acid every day; Example 2 was tested to feed a nutritional paste with 2% glutamine and 1% chlorogenic acid every day; Comparative Example 1 was tested to feed a nutritional paste with 1.5% chlorogenic acid every day; Comparative Example 2 was tested to feed a nutritional paste with 1.5% glutamine every day; Comparative Example 3 was tested to feed a nutritional paste with 0.5% glutamine and 0.25% chlorogenic acid every day; Comparative Example 4 was tested to feed a nutritional paste with 1% glutamine and 0.5% commercially available chlorogenic acid every day. The experimental period was 21 days, with the first day after surgery being recorded as day 0. During the experiment, cats in each group were kept in the same growth environment, with consistent temperature, humidity and other factors. The same diet was selected for daily feeding, and the drinking water source was ensured to be sufficient and clean. The same basic care was performed after surgery, and each group was disinfected after 1 week. This ensured that the impact of the environment on wound healing was as consistent as possible. Any adverse events, including disease occurrence, abnormal behavior, etc., were also recorded during the experiment.

[0053] Example 1 1. Formula of nutritional paste: (1) Basic ingredients: 350 parts of purified water, 150 parts of maltose, 200 parts of chicken (cooked and crushed), 50 parts of carrots, 50 parts of fish oil, 50 parts of glucose, and 50 parts of whole milk powder.

[0054] (2) Additive ingredients: Vitamin A 156000 IU / kg, Vitamin B1 372.3 mg / kg, Vitamin B2 30 mg / kg, Vitamin B6 156 mg / kg, Vitamin D3 9000 IU / kg, Vitamin E 1300 IU / kg, Niacin 50 mg / kg, Folic acid 20.6 mg / kg, D-calcium pantothenate 31 mg / kg, Potassium iodide 26 mg / kg, Manganese sulfate 21 mg / kg, Magnesium sulfate 0.01%, Copper sulfate 36 mg / kg, Ferrous lactate 50 mg / kg, Sodium carboxymethyl cellulose 1%.

[0055] (3) 1% glutamine, 0.5% chlorogenic acid.

[0056] 2. Extraction of chlorogenic acid: Eucommia leaves are used as raw materials for the extraction of chlorogenic acid, and the extraction process of chlorogenic acid is as follows: first, the Eucommia leaves are cleaned, dried and crushed into coarse powder, and then coarsely screened with a 40-mesh sieve to remove impurities and platycodon, and then ultrafinely ground for 20 minutes to grind it into fine powder with a particle size of 1-10 microns; second, a 70% ethanol-water solution is prepared, and the ultrafinely ground Eucommia powder is placed in a reflux extraction device at a solid-liquid ratio of 1:10, and 70% ethanol-water solution is added. Set the temperature to 60°C and the time to reflux extraction for 2.5 hours, and collect the extract; third, use a vacuum concentration device to concentrate the extract at about 40°C to remove most of the ethanol and water; fourth, use a C18 column purification column, gradient elution, the starting solvent is water, and then gradually increase the ethanol ratio to the final pure ethanol, with a flow rate of 1-2 ml / min; fifth, collect different components, and use HPLC to detect and determine the component with the highest chlorogenic acid content; sixth, place the chlorogenic acid solution in a freeze dryer for freeze drying, maintain a low-temperature vacuum environment, gradually increase the temperature, remove moisture, and obtain dry chlorogenic acid powder.

[0057] 3. Preparation of nutritional paste: In the first step, the chicken is cooked, crushed and then passed through a 6-mesh sieve, the carrot raw material is inspected, cleaned, crushed and then passed through a 6-mesh sieve, and after crushing, purified water, maltose, fish oil, glucose and whole milk powder are added, and then homogenized and emulsified at 75° C. for 40 minutes to obtain a first emulsified material; In the second step, the first emulsified material is added with vitamins, minerals and sodium carboxymethyl cellulose, and then homogenized and emulsified at 75° C. for 20 minutes to obtain a second emulsified material.

[0058] The third step is to add 1% glutamine and 0.5% chlorogenic acid powder to the second emulsified material, and homogenize and emulsify at 60° C. for 20 minutes to obtain a nutritional paste product.

[0059] Example 2 The difference from Example 1 is that in the third step, the amount of chlorogenic acid added to the second emulsified material is 1%, and the amount of glutamine added is 2%.

[0060] Comparative Example 1 The difference from Example 1 is that in the third step, 1% of chlorogenic acid is additionally added to the second emulsified material, and glutamine is not added.

[0061] Comparative Example 2 The difference from Example 1 is that in the third step, 0.5% of glutamine is additionally added to the second emulsified material, and chlorogenic acid is not added.

[0062] Comparative Example 3 The difference from Example 1 is that in the third step, the amount of chlorogenic acid added to the second emulsified material is 0.25%, and the amount of glutamine added is 0.5%.

[0063] Comparative Example 4 The difference from Example 1 is that the chlorogenic acid in the second emulsified material is a commercially available product.

[0064] Experimental Results

[0065] Determination of weight loss and weight gain after sterilization in cats 1. Determination method: The cats in the blank control group, Example 1, Example 2 and Comparative Examples 1-4 were weighed on days 1, 7, 14 and 21 after surgery, and the weight loss on day 7, weight gain on day 14 and weight gain on day 21 were calculated compared with the weight loss on day 1 after surgery.

[0066] 2. Test results: according to Figure 1 The results show that the control group 1 with only 1% glutamine added and the control group 2 with only 0.5% chlorogenic acid added showed a higher weight loss in the first 7 days after surgery compared with the other groups. In addition, the weight gain was lower on the 14th and 21st days after surgery, indicating that the recovery after surgery was poor.

[0067] Comparative Example 1, which only added 1% glutamine, and Comparative Example 2, which only added 0.5% chlorogenic acid, showed higher weight loss in the first 7 days after surgery, indicating that Comparative Example 1 and Comparative Example 2 had a low degree of relief of the inflammatory response caused by surgical trauma, which may be detrimental to weight recovery in the short term.

[0068] 14 days and 21 days after surgery, compared with Example 1, Comparative Example 3 and Example 2, the weight gain of Comparative Example 1 and Comparative Example 2 was lower, which was due to postoperative pain, the effects of anesthetic drugs, and digestive system discomfort caused by surgery, further indicating that the overall recovery of the groups adding glutamine or chlorogenic acid alone after surgery was not as good as that of the groups adding both components at the same time.

[0069] In general, the regimen of adding glutamine or chlorogenic acid alone did not show a significant advantage in postoperative recovery, and may even be detrimental to postoperative weight recovery in some cases; while Example 1, which added 1% glutamine and 0.5% chlorogenic acid at the same time, and Example 2, which added 2% glutamine and 1% chlorogenic acid at the same time, showed lower weight loss in the first 7 days after surgery and higher weight gain on the 14th and 21st days after surgery, indicating that the simultaneous addition of 1% glutamine and 0.5% chlorogenic acid or 2% glutamine and 1% chlorogenic acid is more conducive to postoperative weight recovery and has achieved a significant synergistic effect.

[0070] Determination of pain scores after sterilization in cats 1. Evaluation Method Table 1 was used to evaluate the postoperative wound pain index of each group of cats on days 1, 7, 14, and 21 after surgery. Scoring method: The scores of each scoring dimension were added together to obtain the total score. The higher the total score, the lower the pain index. The pain level can be divided into different levels according to the total score: 0-5 points: severe pain; 6-10 points: extreme pain; 11-15 points: severe pain; 16-20 points: moderate pain; 21-30 points: mild pain.

[0071] Table 1: Post-neutering wound pain index score sheet for cats (based on sensory and mental status)

[0072] 2. Evaluation Results from Figure 2 It can be seen that there was no significant difference between the groups on the first day of the experiment. As the experiment continued, there was still no significant difference between Example 3, which added 0.25% chlorogenic acid combined with 0.5% glutamine, and the blank control group, indicating that Example 3 was poor in relieving postoperative pain in pets.

[0073] Compared with the blank control group, Example 1 in which 0.5% chlorogenic acid was added in combination with 1% glutamine and Example 2 in which 1% chlorogenic acid was added in combination with 2% glutamine showed significant differences on the 14th and 21st days, indicating that Example 1 and Example 2 were effective in relieving postoperative pain in pets, and the combination of 0.5% chlorogenic acid in combination with 1% glutamine or 1% chlorogenic acid in combination with 2% glutamine achieved significant synergistic effects.

[0074] Determination of wound inflammation and swelling scores after sterilization in cats 1. Evaluation Method On the 1st, 7th, 14th and 21st days after surgery, the blank control group, Example 1, Example 2 and Comparative Examples 1-4 were scored for inflammation and swelling of the wound after sterilization according to Table 2. The total score is the accumulation of the scores of the following items. The higher the score, the better the wound recovery. According to the total score, it can be divided into different levels: 18-15 points: The wound has recovered well and routine care continues. 14-11 points: There are slight problems with the wound, and it is recommended to strengthen care and observation. 10-6 points: There are moderate problems with the wound, and additional treatment and close observation are required. 5-0 points: There are severe problems with the wound, and immediate medical treatment is required.

[0075] Table 2: Scoring table for wound inflammation and swelling after sterilization of cats

[0076] 2. Evaluation Results from Figure 3 It can be seen that compared with the blank control group and comparative example 3, Example 1 and Example 2 have shown the effect of reducing inflammation and wound swelling on the 7th day.

[0077] As the experiment continued, on the 14th and 21st days of the experiment, the scores of Example 1 and Example 2 were significantly higher than those of the blank control group, indicating that Example 1 with the addition of 0.5% chlorogenic acid combined with 1% glutamine and Example 2 with the addition of 1% chlorogenic acid combined with 2% glutamine achieved significant synergistic effects in reducing the degree of wound swelling.

[0078] Determination of wound diameter after sterilization in cats 1. Evaluation Method (1) Take a photo of the wound immediately after the sterilization surgery (0 days), making sure the photo includes a calibrated ruler for reference. Clean the wound area with sterile saline, making sure there is no blood or other interference when taking the photo. Take a frontal photo of the wound, making sure the camera is perpendicular to the wound plane and the light is even. At the same time, take photos and measure the wound on days 7, 14, and 21 after surgery to record the change in wound area.

[0079] (2) Image acquisition: Throughout the experiment, wound photos were taken repeatedly on days 0, 7, 14, and 21 after surgery. The same lighting conditions and camera settings were used for each shot to ensure image consistency. The wound should be cleaned before each photo was taken, and a calibrated ruler should be used for reference.

[0080] (3) Image analysis: Import the wound photos into the image analysis software (ImageJ). Use the ruler calibration function in the software to calibrate the image to ensure the accuracy of the measurement. Use the area selection tool in the software to outline the wound edge and calculate the wound diameter (cm).

[0081] 2. Evaluation Results Depend on Figure 4 and Figure 5 It can be seen that the test found that compared with Example 1, the length of the postoperative wound in Comparative Example 4 was significantly longer on the 7th day, the 14th day, and the 21st day, indicating that the chlorogenic acid extracted from Eucommia ulmoides can effectively reduce the length of the wound.

[0082] Detection of blood routine indexes after cat sterilization 1. Evaluation Method Blood samples were collected from the blank control group and the cats in the example on the 1st and 21st day after surgery, and blood routine tests were performed immediately using an automatic blood cell analyzer. The test indicators included: total white blood cell count, lymphocyte ratio, intermediate cell ratio, granulocyte ratio, lymphocyte, intermediate cell, granulocyte, total red blood cell count, hemoglobin, hematocrit, mean red blood cell volume, hemoglobin content, hemoglobin concentration, total platelet count, mean platelet volume, platelet distribution width, platelet hematocrit, and platelet-macrocellular ratio.

[0083] Table 3: Blood routine test results after cat sterilization

[0084] 2. Evaluation Results As shown in Table 3, compared with the blank control group, the total white blood cell count, lymphocyte ratio and lymphocyte count in the blood of the cats in Example 1 were significantly reduced 21 days after sterilization ( P ≤0.05), indicating that inflammation in the pet's body has been significantly alleviated; in addition, the total number of red blood cells, hematocrit and hemoglobin content have been significantly improved ( P ≤0.05), indicating that the nutritional status of the pet has been improved, the physical health has been improved, and the recovery after surgery is good. The above results further show that Example 1, which adds 0.5% chlorogenic acid and 1% glutamine, has a significant synergistic effect in relieving inflammation in the pet, improving the nutritional status of the pet, improving the physical health of the pet, and the recovery after surgery of the pet.

[0085] Determination of serum wound healing related indexes 1. Evaluation Method Serum samples were collected from the blood of the blank control group and the cats in Example 1 on the 1st and 21st day after surgery, and the specific determination method of serum epidermal growth factor (EGF), fibronectin (FN) and transforming growth factor-β1 (TGF-β1) in the serum was detected by referring to the ELISA kit (Shanghai ELISA Biotechnology Co., Ltd.).

[0086] 2. Evaluation Results Wound healing is a complex physiological process involving the interaction between multiple cytokines and extracellular matrix, among which serum epidermal growth factor (EGF), transforming growth factor (TGF-1β) and fibronectin (FN) are considered to be important factors involved in regulating cell proliferation and differentiation and affecting wound healing. Figure 6 It can be seen that compared with the control group, the serum levels of epidermal growth factor (EGF), transforming growth factor (TGF-1β) and fibronectin (FN) in cats in Example 1 were significantly increased 21 days after sterilization ( P ≤0.05), indicating that the nutritional paste in Example 1 can promote wound repair by increasing the content of factors related to regulating cell proliferation and differentiation in pets.

Claims

1. A composition for promoting wound healing of pets, It is characterized in that The composition comprises chlorogenic acid and glutamine, wherein the mass proportion of the chlorogenic acid in the composition is 0.2-1.5%; the mass proportion of the glutamine in the composition is 0.5-2.5%.

2. The composition according to claim 1, It is characterized in that The composition also includes basic raw materials, which account for 93.5-97.5% of the mass of the basic raw materials in the composition. The basic raw materials include the following components in parts by weight: 100-200 parts of maltose, 150-250 parts of chicken, 30-70 parts of carrots, 30-70 parts of fish oil, 30-70 parts of glucose, and 30-70 parts of whole milk powder.

3. The composition according to claim 1, It is characterized in that The composition also includes additives, the mass proportion of the additives in the composition is 1-5%, and the additives include vitamin A 100000-200000 IU / kg, vitamin B1 300-400 mg / kg, vitamin B2 10-50 mg / kg, vitamin B6 100-200 mg / kg, vitamin D 3 8000-10000IU / kg, vitamin E 1000-1600 IU / kg, niacin 10-90mg / kg, folic acid 10-90mg / kg, D-pantothenate calcium 10-90mg / kg, potassium iodide 10-90mg / kg, manganese sulfate 10-90mg / kg, magnesium sulfate 0.005-0.1%, copper sulfate 10-50mg / kg, ferrous lactate 10-90mg / kg, sodium carboxymethyl cellulose 0.5-1.5%.

4. A method for preparing the composition according to claim 1, It is characterized in that The preparation method comprises the following steps: S1. Cook the chicken and wash the carrots in the basic raw materials, crush them, and sieve them. Then, mix the sieved chicken and carrots with other basic raw materials, and perform homogenization and emulsification treatment to obtain a first emulsified material. S2: adding additives to the first emulsified material, performing homogenization and emulsification treatment, and obtaining a second emulsified material; S3: adding glutamine and chlorogenic acid to the second emulsified material, performing homogenization and emulsification treatment, and obtaining a composition.

5. The preparation method according to claim 4, It is characterized in that The homogenization and emulsification temperature in step S1 is 70-80°C; The homogenization and emulsification temperature in step S2 is 70-80°C; The homogenization and emulsification temperature in step S3 is 55-65°C.

6. The preparation method according to claim 4, It is characterized in that The homogenization and emulsification time in step S1 is 35-45 minutes; the homogenization and emulsification time in step S2 is 15-25 minutes; and the homogenization and emulsification time in step S3 is 15-25 minutes.

7. Use of the composition according to any one of claims 1 to 3 or the composition prepared by the preparation method according to any one of claims 4 to 6 in preparing a product for promoting the recovery of pets.

8. The use according to claim 7, It is characterized in that The pets are cats, dogs, minks, hamsters, rabbits and meerkats.

9. The use according to claim 7, It is characterized in that The product is pet food, medicine or toiletries.

10. The use according to claim 7, It is characterized in that The methods of promoting pet recovery include: (1) Reduce postoperative weight loss; (2) Reduce postoperative wound pain; (3) Reduce inflammation of skin wounds; (4) Reduce the swelling of the skin wound; (5) Reduce wound diameter; (6) Improve inflammation in the body; (7) Promote wound healing.

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