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Antithrombin III determination kit

An antithrombin and kit technology, applied in the field of antithrombin III assay kits, can solve the problems of affecting the numerical value of assay results, deviation of final results, slow assay rate of assay kits, etc.

Inactive Publication Date: 2020-10-27
BAODING TIANYUE BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the disadvantages of the measurement kits in the prior art that the measurement rate is relatively slow, and the reagent reaction is still in progress after the measurement is completed, which affects the value of the measurement experiment results and leads to deviations in the final results. A Proposed Antithrombin III Assay Kit

Method used

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Effect test

Embodiment 1

[0032] An antithrombin III assay kit proposed by the present invention includes reagent one, reagent two, speed-increasing liquid and stop liquid;

[0033] Reagent 1: the concentration of thrombin is 5IU / ml, the content of heparin is 0.8wt%, and the content of salt is 0.25wt%;

[0034] In reagent two: the content of the chromogenic substrate of thrombin is 2.5wt%, and the content of mannitol is 0.3wt%;

[0035] The concentration of disodium hydrogen phosphate solution in the speed-up liquid is 1IU / ml;

[0036] In the stop solution: the content of polyvinyl chloride resin is 0.3wt% and the content of sodium azide is 0.5wt%.

[0037] In this embodiment, the chromogenic substrate is one of S2238, S-2160 and Chromozym TH, the buffer is Tris, and the salt is one or more of sodium chloride, potassium chloride, magnesium chloride, and ammonium sulfate.

[0038] Its use method includes the following steps:

[0039] S1: Take the required detection solution and put it in the first re...

Embodiment 2

[0045] An antithrombin III assay kit proposed by the present invention includes reagent one, reagent two, speed-increasing liquid and stop liquid;

[0046] In reagent one: the concentration of thrombin is 10IU / ml, the content of heparin is 0.9wt%, and the content of salt is 0.3wt%;

[0047]In reagent two: the content of the chromogenic substrate of thrombin is 3.0wt%, and the content of mannitol is 0.4wt%;

[0048] The concentration of disodium hydrogen phosphate solution in the speed-up liquid is 2IU / ml;

[0049] In the stop solution: the content of polyvinyl chloride resin is 0.5wt% and the content of sodium azide is 0.6wt%.

[0050] In this embodiment, the chromogenic substrate is one of S2238, S-2160 and Chromozym TH, the buffer is Tris, and the salt is one or more of sodium chloride, potassium chloride, magnesium chloride, and ammonium sulfate.

[0051] Its method of use includes the following steps:

[0052] S1: Take the required detection solution and put it in the f...

Embodiment 3

[0058] An antithrombin III assay kit proposed by the present invention includes reagent one, reagent two, speed-increasing liquid and stop liquid;

[0059] In reagent one: the concentration of thrombin is 15IU / ml, the content of heparin is 1.1wt%, and the content of salt is 0.4wt%;

[0060] In reagent two: the content of the chromogenic substrate of thrombin is 3.5wt%, and the content of mannitol is 0.4wt%;

[0061] The concentration of disodium hydrogen phosphate solution in the speed-up liquid is 2IU / ml;

[0062] In the stop solution: the content of polyvinyl chloride resin is 0.8wt% and the content of sodium azide is 0.7wt%.

[0063] In this embodiment, the chromogenic substrate is one of S2238, S-2160 and Chromozym TH, the buffer is Tris, and the salt is one or more of sodium chloride, potassium chloride, magnesium chloride, and ammonium sulfate.

[0064] Its method of use includes the following steps:

[0065] S1: Take the required detection solution and put it in the ...

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Abstract

The invention relates to the technical field of in-vitro diagnosis, and particularly relates to an antithrombin III determination kit, and overcomes the defects that the determination rate of a determination kit in the prior art is low, and after determination is completed, a reagent reaction is still carried out continuously, so that the numerical value of a determination experiment result is influenced, and a final result is deviated. The antithrombin III determination kit comprises a reagent I, a reagent II, a speed-up solution and a stop solution, wherein the reagent I comprises thrombin,heparin, a buffer solution and salt; the reagent II comprises a chromogenic substrate of the thrombin, a buffer solution and mannitol; and the speed-up solution comprises a disodium hydrogen phosphatesolution; the stop solution comprises polyvinyl chloride resin and sodium azide. The reagent I, the reagent II, the speed-up solution and the stop solution are combined to form the kit, the antithrombin III is determined quickly by utilizing the reagent I, the reagent II and the speed-up solution, and the reaction is stopped by utilizing the stop solution after determination is completed, so thatdata deviation caused by slow and continuous reaction is avoided; the whole determination kit is convenient to use, quick in determination and accurate in data.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to an antithrombin III assay kit. Background technique [0002] According to the principle of naming medical devices, product names should be unified. Currently, there are two types of product names on the market, antithrombin III assay kit (chromogenic substrate method) and antithrombin assay kit (chromogenic substrate method) substrate method). [0003] Antithrombin international standard substance is prepared and assigned by the British National Institute for Biological Products Control (NIBSC). According to the information provided by the agency, the first-generation international reference product (72 / 1) established by the World Health Organization (WHO) in 1978 was named antithrombin III. The nomenclature follows Seegers WH, Johnson JF, Fell C (1954) publication "Prothrombin Activated Antithrombin Response", Am. J. Physiol. 176(1):97-103. In this article, there a...

Claims

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Application Information

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IPC IPC(8): C12Q1/56
CPCC12Q1/56G01N2333/8128
Inventor 焦东燕刘芳王玉玉
Owner BAODING TIANYUE BIOENG
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