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A kind of antithrombin Ⅲ assay kit and its detection method
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A technology of antithrombin and reagent kits, applied in the field of biomedical testing, can solve problems such as large application limitations, complicated operation, and poor stability, and achieve the effects of strong anti-interference ability, wide application prospects, and good stability
Active Publication Date: 2020-06-19
NINGBO RUI BIO TECH
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[0005] However, the chromogenic substrate method also has certain defects. This method is to analyze the AT-III activity in the sample by adding exogenous thrombin, because there is also another heparin-catalyzed thrombin inhibitor—heparin cofactor II in the sample. (HCII), so the activity of determining the remaining thrombin is the remaining activity of thrombin after reacting with AT-III and HCII, therefore, this method is susceptible to the interference of HCII in the sample, resulting in inaccurate determination of AT-III
[0006] Currently commercially available reagents mainly use heparinase to degrade heparin to reduce the interference of HCII, but the operation is complicated and the heparinase is expensive, which greatly increases the cost
The other is through the coagulation factor Xa pathway. Although it avoids the interference of heparin cofactor II, the coagulation factor Xa is not only expensive, but also has poor stability. The sensitivity of the reagent is not as high as that of the thrombin pathway, and its application is very limited.
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Embodiment 1
[0039] The antithrombin III assay kit of this embodiment consists of the following components:
[0056] Sodium azide 1g / L, HD-Phe-Pip-Arg-pNA·2HCl 2g / L.
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Abstract
The invention belongs to the technical field of biomedical examination, and relates to an antithrombin III determination kit and a detection method thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, a stabilizer, a salt, a surfactant, a preservative, trehalose, heparin and thrombin; and the reagent R2 comprises the buffer solution, the stabilizer, the surfactant, the preservative and a chromogenic substrate. A sample and deionized water are mixed according to a volume ratio of 1:(3-5) to obtain a diluted sample; and the diluted sampleis incubated for 30-60 s, the reagent R1 is added, uniform mixing and incubation are carried out for 60-100 s, the reagent R2 is added, uniform mixing is carried out, and then the obtained mixture isdetected at a wavelength of 405 nm.
Description
technical field [0001] The invention belongs to the technical field of biomedical testing, and relates to an antithrombin III assay kit and a detection method thereof. Background technique [0002] Antithrombin Ⅲ (AT-Ⅲ) is a single-chain glycoprotein, which belongs to α2 globulin. AT-Ⅲ has the function of decomposing lipoproteins and inhibits almost all active serineproteases in the coagulation system. AT-III, as the most important inhibitor of active coagulation factors in blood, controls blood coagulation and fibrindissolution. The level of antithrombin III in the blood varies depending on various diseases and symptoms. Reduced in disseminated intravascular coagulation (DIC), liver disease, nephrotic syndrome, etc. Decreased levels of antithrombin III in the blood may prevent the effect of heparin therapy. Therefore, it is of great significance to know the activity of antithrombin III as an index for the monitoring, pathological analysis, prognosis judgment and hepari...
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