A kind of indirect ELISA detection kit and detection method for antibody of Pasteurella multocida of bovine origin

A technology for detection kits and Pasteurella, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of high biological safety, high homology, and high homology
CN106053807BActive Publication Date: 2018-03-27SOUTHWEST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTHWEST UNIV
Publication Date
2018-03-27

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Abstract

The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) detection reagent kit and a detection method for bovine-derived pasteurella multocida antibodies. The indirect ELISA detection reagent kit comprises solid-phase carriers enveloped by bovine-derived pasteurella multocida specific protein 0230, HRP (horse radish peroxidase)-labeled IgG (immunoglobulin G) enzyme-labeled second antibodies, standard positive control serum, standard negative control serum, sample diluent, concentrated washing solution, color developing solution and stop solution. The detection method is implemented by the aid of the indirect ELISA detection reagent kit, and includes enabling to-be-detected serum samples to be in contact with the enveloping bovine-derived pasteurella multocida specific protein 0230 on the solid-phase carriers and carrying out heat-insulation incubation; specifically capturing specific antibodies combined on 0230 protein antigens by the aid of the HRP-labeled IgG enzyme-labeled secondary antibodies; developing colors by the aid of color developing agents so as to measure 0230 protein antibody level of the to-be-detected serum samples. The indirect ELISA detection reagent kit and the detection method have the advantages of good specificity and repeatability, high sensitivity and the like.
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Description

technical field

[0001] The invention relates to the field of veterinary biological products, in particular to an indirect ELISA detection kit and a detection method for a bovine Pasteurella multocida antibody based on a specific protein. Background technique

[0002] Pasteurella multocida (Bovine Pasteurella multocida , Pm) is an important pathogen causing hemorrhagic sepsis or respiratory diseases in cattle. Pasteurella multocida can be divided into five capsular serotypes, A, B, D, E, and F, according to their capsular antigens. Among them, the capsular serotypes that are pathogenic to cattle include A, B, and F. Types E and F; Types A and F mainly cause respiratory diseases in cattle, and types B and E mainly cause bovine hemorrhagic sepsis. Pasteurella multocida, which was prevalent in my country in the past, was mainly caused by capsular serum type B. Since 2008, pasteurella multocida type A has been prevalent in many provinces and cities in my country, causing seriou...

Claims

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