4results about How to "Rapid typing" patented technology

This invention discloses EST-SSR molecular markers for *Paphiopedilum florida* and *Paphiopedilum simonii*, along with their usage and applications. The nucleotide sequences of the corresponding primer sets are shown in SEQ ID NO:2-3, SEQ ID NO:5-6, SEQ ID NO:8-9, SEQ ID NO:11-12, SEQ ID NO:14-15, and SEQ ID NO:16-65. The primer sets in this invention can be widely used for typing and identifying any *Paphiopedilum* species, overcoming the shortcomings of poor primer universality in existing technologies, and achieving batch and rapid typing of *Paphiopedilum* species.

The present application belongs to the technical field of molecular markers and animal breeding, and particularly relates to a molecular marker related to the content of glycine, alanine and histidine in beef cattle muscle and application thereof. The present application provides a molecular marker located at the 18184950th base of chromosome 17 of a beef cattle reference genome GCF_002263795.3 version, having C / T polymorphism, which can not only realize rapid and accurate genotyping of beef cattle population SETD7 gene, but also based on the significant correlation between the site and the amino acid content of individuals, the beef cattle individuals with high amino acid content can be screened early, so as to improve the selection speed of superior beef cattle. Compared with the traditional single genotype selection, the present application has higher accuracy and significant advantages for screening beef cattle individuals with high amino acid content, saving feeding cost and improving the selection of superior beef cattle. The amino acid in the present application is one or more of glycine, alanine and histidine.

The invention discloses a meat duck body length character molecular marker as well as an identification primer and application thereof. The molecular marker is a duck No.28 chromosome chr28: 301790 locus, the locus has a T / C allele, and the corresponding genotypes are TT, TC and CC. The invention further provides an allele specific primer combination based on the KASP technology, the allele specific primer combination comprises an FAM primer, a VIC primer and a universal primer, and the specific sequences of the FAM primer, the VIC primer and the universal primer are shown as SEQ ID NO.1-3. According to the method, marker-assisted selection is carried out on 10 commercial lines of Huaifu meat ducks, TT type individuals are preferentially selected and reserved, CC type individuals are eliminated, the semi-submersible length is increased by 3.95 cm, the body weight is increased by 200 g, and the progress rate of breeding generations is increased by 38% or above. The method is simple and convenient to operate, low in cost and high in accuracy, and a molecular tool and a technical support are provided for early-stage precise breeding of body length characters of Huaifu meat ducks and similar strains.

This invention relates to the field of molecular biology, specifically to SNP molecular markers associated with palmitoleic acid trait in sheep muscle and their applications. Based on the detection of exons in the sheep SMAD1 gene, this invention identifies an SNP site at position 493 of exon 2 of the SMAD1 gene or position 13,440,579 of chromosome 17 of sheep. The base at this site is either A or G, and allele A is positively correlated with high palmitoleic acid trait in muscle. Furthermore, a method for detecting this SNP molecular marker is provided to achieve rapid and accurate genotyping of the SMAD1 gene in sheep populations (e.g., double-dried meat sheep). Moreover, based on the significant correlation between this site and individual palmitoleic acid and fatty acid content, SNP markers can be used to screen sheep individuals with higher palmitoleic acid content at an early stage, thereby improving the speed of breeding superior sheep.