68results about How to "Low mutation rate" patented technology

An anti-aging method and composition are disclosed. The composition is a micro-cluster water having anti-oxidant and free-radical quenching properties. The method involves contacting a cell or portion thereof in danger of free-radical or oxidant damage with the micro-water.

The invention relates to the technical field of molecular biology, and especially relates to a multiplex amplification system, and a next generation sequencing typing kit and typing method for 21 micro-haplotype sites. The 21 micro-haplotype sites in the multiplex amplification system are derived from 21 autosomes, are balanced in allele frequency, are mutually independent between the sites, and have the advantages of polymorphism and low mutation rate. The kit contains 53 PCR-amplified single-end specific primers shown in SEQ ID NO. 1-SEQ ID NO.53, and can detect the 21 microhaplotype sites in a same reaction system. The multiplex amplification system, the kit and the typing method provided by the invention can solve the technical problem that a current STR typing technology cannot obtainan ideal typing result, and allele loss may occur due to excessive number of SNP sites.

The invention discloses a genetic marker combination for human individual recognition and/or paternity identification and a detection method thereof. By conducting whole-genome SNPs unbiased scanningon multiple races, a SNPs site combination widely applicable to the multiple races is screened, and 116 autosome SNPs sites which come from 37 groups in different regions in the whole world, have thehypermorph allel frequency and low difference and achieve independent inheritance and 12 X chromosome SNPs sites which come from 37 groups in different regions in the whole world, have the hypermorphallel frequency and low difference and achieve independent inheritance are screened from 25,580,678 SNPs sites in a genome-wide scale. Compared with an existing commercial kit, the SNPs site combination has higher and wider multiracial adaptation, the cumulative individual recognition probability and the cumulative probability of exclusion are both significantly superior to those of the commercialkit, the detection result is more accurate, and a great application prospect is achieved.

The invention discloses a compound amplification detection kit containing forty InDel genetic polymorphic sites of human X chromosome. The forty InDel sites of the X chromosome and an individual identification location point are amplified simultaneously by a primer group. Compared with a traditional STR genetic marker, an insertion deletion genetic marker used in the compound amplification detection kit has the advantages of low mutation rate and high sensitivity; and the polymorphism detection sites involved in the compound amplification detection kit combine the advantages of an InDel genetic marker and the special genetic characteristics of the X chromosome, and effective supplementary means can be provided for identification of difficult or special cases.

The invention provides a quick detection kit for a chicken-origin ingredient in food and feed and relates to the technical field of biological species identification. A general chicken-origin internal standard gene Actb is screened first; a nucleotide sequence of the gene Actb is as shown as SEQ ID NO.1 (Sequence Identifier Number 1); the gene Actb is located on a chromosome; single copy is easy to quantify; and the gene Actb has a constant copy number in chicken species, without allelic variation and can serve as a target gene for chicken-origin identification. LAMP amplification primers are designed by taking the gene as a target sequence; nucleotide sequences of the LAMP amplification primers are as shown as SEQ ID NO. 2-5; the LAMP amplification primers and an LAMP reaction solution constitute the detection kit; the detection kit can detect the chicken-origin ingredient in the food and the feed quickly and sensitively, and the sensitivity reaches 10pg. The kit is simple in use method, low in cost, high in specificity and very applicable to field real-time detection and has a wide application prospect in animal-origin food surveillance and meat product adulteration defense, and a reaction result is easy to observe.

The invention discloses a primer-free gene synthesis method and belongs to the field of artificial synthesis of genes. The invention discloses a pNew carrier plasmid, and the pNew carrier plasmid has a nucleotide sequence shown in the formula of SEQ ID NO.33. The invention also discloses a carrier bank which is constructed from pNew carrier plasmids and contains 16384(47) plasmids. The invention further discloses a primer-free gene synthesis method. The primer-free gene synthesis method comprises the following steps of 1, carrying out grouping on targeted gene DNA sequences to be synthesized according to a ratio of 82bp per fragment, 2, finding plasmids corresponding to the grouped fragments in the constructed plasmid bank, 3, carrying out enzyme digestion on the corresponding plasmids, carrying out screening by antibiotic and carrying out reconstruction to obtain a plasmid containing 82bp of the targeted gene sequences, and 4, splicing the gene fragments by a golden-gate cloning reaction to obtain a complete targeted gene. The primer-free gene synthesis method is free of primers, has a low synthesis cost, a very low mutation rate and high accuracy, can synthesize special gene sequences such as a highly repetitive sequence and Poly A, has simple processes and can realize automatic operation.

A rational method for obtaining a novel molecule capable of a desired interaction with a substrate of interest comprising selecting hosts or replicators which encode said novel molecules based upon cell or replicator growth caused by the desired interaction of the novel molecule and a selection molecule expressed by said host.

The invention belongs to the field of new research of forensic medicine, and particularly relates to a composite amplification typing detection system kit and a detection method for 35 new insertion/deletion (InDel) sites in forensic individual identification research. According to the present invention, the primers of InDel sites are respectively subjected to fluorescent labeling with four fluorescent substances (FAM, HEX, TAMRA and ROX), such that the 35 sites are subjected to composite amplification in the same amplification system, and are detected synchronously; the InDel sites are the length polymorphism molecular genetic markers, can be typed by using the capillary electrophoresis method, and are easily applied and promoted in the basic forensic department; the kit is compatible with various biological samples, and has advantages of high sensitivity, strong specificity and simple and quick operation; and the 35 InDel sites have high cumulative individual recognition rate in unrelated healthy individuals, can meet the needs of forensic individual identification, and further can provide new and effective detection reagents for forensic practice.

The invention discloses an X-STR locus fluorescence labeling composite amplification system and an application thereof. The composite amplification system comprises 14 loca in total: DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, GATA31E08, DXS10074 and DXS10079. The 14 X-STR loca are amplified simultaneously in a composite amplification system in a fast and convenient manner; the PCR product can be standardized and internationalized and ensure relative accuracy of different lab data with genetic analyzer electrophoresis and automatic result analysis.

The invention provides a primer-free gene synthesis method based on a plasmid library, and the method is as follows: constructing a plasmid library containing 16384 plasmids (any combination sequence of four basic groups of 47 and 7bp), starting from a constructed plasmid containing 7 bp, dividing a selected target gene according to each 82bp, successively reconstructing four resistance genes Kana, cam, gem and spc by enzyme digestion and enzyme connection operation to obtain a plurality of 82bp plasmids, and assembling the 82bp plasmids into long fragment genes. According to the method, after construction of the plasmid library containing the 16384 plasmids (any combination sequence of four basic groups of 47 and 7bp), various target genes can be synthesized without any primer. The synthesis method has the advantages of simple operation, extremely low mutation rate, no need of sequencing, low cost and less amount of screening, is conductive to constructing a mutant library, can realize solution operation, and is easy to realize automation.

The invention discloses a cultivation method of dendrobium officinale group. The cultivation method of dendrobium officinale group includes the following steps in order: 1) breeding: a. protocorm induction, b. protocorm differentiation, c. bud proliferation, d. seedling strengthening and rooting, 2) seedling selection, 3) removal out of a bottle, 4) cultivation, and 5) field management prevention and control of diseases and pests. The cultivation method provided by the invention has the advantages of high seed germination rate, low mutation rate, high seedling survival rate, good growth vigor, low lesion rate, and high economic benefits.

The invention discloses a method for identifying the human-derived genetic relationship through a two-nucleotide repeated microsatellite. The method comprises the step that through primer design, primer modification, PCR amplification and capillary electrophoresis detection, the human-derived genetic relationship is identified according to capillary electrophoresis detection results. According to the method, the number of adopted sites is large, and the identification accuracy is high; compared with traditional tetranucleotide repeated STR, the two-nucleotide repeated STR is lower in mutation rate, and the identification reliability is improved; the primer for amplification is modified, so that the identification results are more clear and obvious; and the method can be used for investigation of crime, paternity test or human-derived cell identification.

The invention relates to a pharmaceutical composition for treating tumors or cancers and application thereof, in particular to a pharmaceutical composition which comprises vesicular stomatitis virusesdelivered through direct injection or systemic administration or intratumoral delivery and is combined with PDL1 antibody intravenous administration to generate a synergistic effect in treatment of metastatic lung cancer. Besides, the oncolytic virus after gene mutation and the PDL1 antibody have the characteristic of recognizing tumor cells, normal cells are not damaged, and the oncolytic virusand the PDL1 antibody are sequentially combined for use to generate a synergistic effect in curative effect.

The invention belongs to the field of forensic new technology research, and particularly relates to a composite amplification typing detection system kit and detection method for suitable for forensicdegradation sample individual identification and sex confirmation application research of 43 insertion/deletion and 1 amelogenin loci. The four different colors of fluorescent substances (FAM, HEX, TAMRA and ROX) are used for carrying out fluorescence labeling grouping on the primers of 44 loci, so that the 44 loci can be simultaneously subjected to composite amplification in the same amplification system. The InDels loci provided by the invention can be used for typing detection of degradation samples due to the fact that amplified fragments are short. The 44 loci contained in the system kithave high accumulated individual identification rate in irrelevant healthy individuals, and can meet the needs of individual identification of forensic degradation biological samples.

The invention provides a fast detection kit of a donkey-origin component in food, and relates to the technical field of biological species identification. According to the fast detection kit of the donkey-origin component in the food, firstly, a donkey-origin universal endogenous reference gene LOC106825524 is screened out, a nucleotide sequence of the donkey-origin universal endogenous referencegene LOC106825524 is as shown in SEQ ID NO.1, and a copy number of the donkey-origin universal endogenous reference gene LOC106825524 in the donkey species is constant so that the donkey-origin universal endogenous reference gene LOC106825524 can be used as a target gene for identifying a donkey origin. By using the gene as a target sequence, PCR amplification primers are designed, the PCR amplification primers and PCR reaction liquid amplify together, and a PCR reaction product is used for platinum-palladium nanoparticle immunochromatographic test strip detection. PCR reaction in combinationwith platinum-palladium nanoparticle-based immunochromatographic test strip detection forms the fast detection kit, the donkey-origin component in the food can be detected fast and sensitively, and the detection sensitivity can reach 0.8%. The kit is simple in use method and low in cost, a reaction result is easy to observe, the specificity is good, and the kit is suitable for on-site real-time detection, and has wide application prospects on the aspects of animal-origin food surveillance and meat product adulteration resistance.

Methods of modifying a frataxin gene are disclosed, comprising removing some or all of endogenous GAA trinucleotide repeats within the frataxin gene, e.g., within an intron (e.g., intron 1) of the frataxin gene. The removal may be effected using a CRISPR/CAS nuclease system. Such modification may be used to increase frataxin expression in the cell, and also to treat a subject suffering from Friedreich ataxia. Reagents, kits and uses of the method are also disclosed, for example to modify a frataxin gene and to treat a subject suffering from Friedreich ataxia.

The invention belongs to the field of forensic medicines, and discloses a system for subtyping 59 micro-haplotypes genetic markers for individual identification in forensic medicine and application thereof. The invention provides a genetic marker combination which is composed of 59 micro-haplotypes positioned on an autosome, and the genetic marker combination can be effectively applied to detection of the forensic medicine; and compared with a conventional detection method, the micro-haplotypes genetic markers for detection in the forensic medicine provided by the invention have the advantagesof being high in sensitivity, accurate in detection results, wide in applicability, and can individually identify human biological samples in a short time, and thus, precious time is gained for breaking forensic cases.

The invention discloses an ancestral polymorphism prediction method based on a big data artificial intelligence algorithm. The method comprises the following steps: S1, selecting thousand-person genome data; S2, screening a plurality of microhaplotype sites from a part of different crowds, and carrying out size classification according to the average effective allele number of the different crowds; S3, carrying out group structure analysis on part of different crowds, and carrying out source classification on the different crowds; and S4, selecting a plurality of microhaplotype sites accordingto the information degree value and the average effective allele value. According to the invention, the microhaplotype is used for detecting and classifying the genes of the crowds, so that the method has the advantages of good high polymorphism, low mutation rate and length, can well predict and analyze the genes and sources of the crowds, and is high in accuracy; a plurality of diallelic SNP loci, a plurality of triallelic SNP loci and a plurality of microhaplotype loci are selected according to indexes such as information degree, fixed indexes and effective allele number, so that the accuracy of ancestral sources is respectively high.

The invention discloses a human DIP-InDel locus fluorescent labeling kit and a detection method thereof. The kit comprises 45 specific multiplex PCR primers with nucleotide sequences shown in SEQ NO.1-45 and 4 pairs of fluorescent labeled universal primers with nucleotide sequences shown in SEQ NO.46-53. By amplifying and comparing 15 DIP-InDel loci, trace components in double-human unbalanced mixed sample are detected and excluded, an individual is confirmed, and the kit can be applied to aspects such as double-human mixed stain medicolegal expertise and non-invasive fetal paternity test.

The invention relates to a method for judging specific individual attribution of malignant tumor tissues. The method comprises the following operating steps: respectively extracting cell DNA of the malignant tumor tissues to be tested and cell DNA of the normal tissues of a specific individual; quantifying and diluting the DNA, and then performing genome sequencing by using a next-generation sequencing technology to obtain original genomic data; performing judgment of single nucleotide polymorphism according to the original genomic data; judging the attribution of the malignant tumor tissues and the specific individual based on the result of the judgment. The method has the characteristics of being high in accuracy, strong in expansibility, rapid and flexible, and the like, and can more accurately detect the genotyping of the malignant tumor tissues, thereby being applied to forensic identification of the malignant tumor tissues; the method can be used for judging whether the malignanttumor tissues to be tested are derived from a specific individual or not.