Fast detection kit of donkey-origin component in food and application thereof

Abstract

The invention provides a fast detection kit of a donkey-origin component in food, and relates to the technical field of biological species identification. According to the fast detection kit of the donkey-origin component in the food, firstly, a donkey-origin universal endogenous reference gene LOC106825524 is screened out, a nucleotide sequence of the donkey-origin universal endogenous referencegene LOC106825524 is as shown in SEQ ID NO.1, and a copy number of the donkey-origin universal endogenous reference gene LOC106825524 in the donkey species is constant so that the donkey-origin universal endogenous reference gene LOC106825524 can be used as a target gene for identifying a donkey origin. By using the gene as a target sequence, PCR amplification primers are designed, the PCR amplification primers and PCR reaction liquid amplify together, and a PCR reaction product is used for platinum-palladium nanoparticle immunochromatographic test strip detection. PCR reaction in combinationwith platinum-palladium nanoparticle-based immunochromatographic test strip detection forms the fast detection kit, the donkey-origin component in the food can be detected fast and sensitively, and the detection sensitivity can reach 0.8%. The kit is simple in use method and low in cost, a reaction result is easy to observe, the specificity is good, and the kit is suitable for on-site real-time detection, and has wide application prospects on the aspects of animal-origin food surveillance and meat product adulteration resistance.

Description

technical field [0001] The invention relates to the technical field of identification of biological species, in particular to a rapid detection kit for detection of donkey-derived components in food combined with a polymerase chain reaction (PCR) technique combined with platinum-palladium nanoparticle immunochromatographic test strips. Background technique [0002] With the rapid development of the economy and the improvement of people's living standards, the demand of Chinese residents for meat products is increasing year by year. Although many countries clearly require food labels to clearly indicate the type and source of meat and prohibit adulteration, there are still many incidents of meat adulteration in the market. For example, in order to reduce costs, donkey meat is mixed with pork , mutton mixed with pork or duck, sausage mixed with other meat and so on. At present, the PCR method is a simple and effective method for detecting food adulteration, which is simple in...

AI Technical Summary

Problems solved by technology

At present, the adulteration detection technology of meat products at home and abroad is mostly based on the design of specific primers for the genes on the mitochondria to perform real-time fluorescent quantitative PCR amplification. Since the mitochondrial genes are multi-copy genes, their detection sensitivity is high, but at the same time it is difficult to distinguish due to sales, transportation Confusion between unintentional cross-contamination and intentional illegal additions

In addition, the concentration of high-copy mitochondrial DNA cannot correspond to the concentration of genomic DNA, so it is impossible to perform accurate quantitative analysis on samples. At the same time, due to the high homology of mitochondrial genes, it is difficult to achieve qualitative detection by ordinary PCR

Therefore, this method can only realize the screening and identification of meat adulteration by means of fluorescent quantitative PCR

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