Genetic marker for human individual recognition and/or paternity identification and detection method thereof and kit

A technology of paternity identification and genetic markers, which is applied in the field of human individual identification and/or genetic markers of paternity identification and its detection methods and kits, and can solve problems such as limited wide applicability

Active Publication Date: 2018-01-05
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to differences in selection preferences of candidate loci and differences in the popula

Method used

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  • Genetic marker for human individual recognition and/or paternity identification and detection method thereof and kit
  • Genetic marker for human individual recognition and/or paternity identification and detection method thereof and kit
  • Genetic marker for human individual recognition and/or paternity identification and detection method thereof and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Verification of 128 SNPs in 134 Chinese samples and comparison with commercial kits

[0077] 1. Research object

[0078] The subjects of the study were 134 peripheral blood samples from Han Chinese, 96 of which were from Guangzhou and 34 from Zhengzhou. All samples signed informed consent forms according to the principle of informed consent.

[0079] 2. Genotyping

[0080] (1) Primer design

[0081] Amplification primers and single-base extension primers were designed for the 128 SNPs of the present invention, and their sequences are shown in Table 1 below, which were synthesized by a primer synthesis company.

[0082] Amplification primers and single base extension primer sequences of 128 SNPs of the present invention in table 1

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[0095] (2) DNA extraction and quality inspection

[0096] Genomic DNA was extracted...

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Abstract

The invention discloses a genetic marker combination for human individual recognition and/or paternity identification and a detection method thereof. By conducting whole-genome SNPs unbiased scanningon multiple races, a SNPs site combination widely applicable to the multiple races is screened, and 116 autosome SNPs sites which come from 37 groups in different regions in the whole world, have thehypermorph allel frequency and low difference and achieve independent inheritance and 12 X chromosome SNPs sites which come from 37 groups in different regions in the whole world, have the hypermorphallel frequency and low difference and achieve independent inheritance are screened from 25,580,678 SNPs sites in a genome-wide scale. Compared with an existing commercial kit, the SNPs site combination has higher and wider multiracial adaptation, the cumulative individual recognition probability and the cumulative probability of exclusion are both significantly superior to those of the commercialkit, the detection result is more accurate, and a great application prospect is achieved.

Description

technical field [0001] The invention belongs to the technical field of forensic genetics. More specifically, it relates to a genetic marker for human individual identification and / or paternity testing, a detection method and a kit thereof. Background technique [0002] DNA evidence is widely used in human individual identification and paternity testing, and plays a key role in scientific evidence in the fields of criminal litigation, civil law cases, disastrous accident handling, and scientific research. The so-called DNA evidence refers to whether the genetic marker genotypes on the chromosomes of different individuals meet the requirements of forensic genetics. At present, DNA identification mainly tests 13 to 20 short tandem repeats (Short tandem repeats, STRs) that are inherited independently of each other, which are called second-generation genetic markers. [0003] The third generation genetic marker—Single nucleotide polymorphisms (Single nucleotide polymorphisms, S...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 黄二文王小广
Owner SUN YAT SEN UNIV
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