44 InDels locus composite amplification detection kit for forensic individual identification of degradation samples

A compound amplification and forensic technology, applied in the field of new forensic science research, can solve the problems of low application value, inability to perform gender confirmation, low polymorphism degree, etc., and achieve the effects of low mutation rate, high sensitivity and simple operation.

Pending Publication Date: 2019-05-17
朱波峰 +3
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  • Abstract
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Problems solved by technology

[0004] Investigator, an InDels typing detection kit commonly used in the world for individual identification ® DIPplex is mainly aimed at European and American populations. Past studies have found that t

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  • 44 InDels locus composite amplification detection kit for forensic individual identification of degradation samples
  • 44 InDels locus composite amplification detection kit for forensic individual identification of degradation samples
  • 44 InDels locus composite amplification detection kit for forensic individual identification of degradation samples

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[0017] The present invention will be exemplified below in conjunction with the accompanying drawings and further detailed description. It should be noted that the following descriptions are merely examples of the technical solutions claimed in the present invention, and are not intended to limit these technical solutions. The protection scope of the present invention is based on the contents described in the appended claims.

[0018] The reagents used in the present invention include: Master mix I, Primer mix, Nuclease-Free Water, Control DNA M308, Size Standard Org500, 5-Dye matrix Standards and deionized formamide solution.

[0019] The primer sequence information of 44 sites in the present invention is as follows:

[0020]

[0021]

[0022]

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Abstract

The invention belongs to the field of forensic new technology research, and particularly relates to a composite amplification typing detection system kit and detection method for suitable for forensicdegradation sample individual identification and sex confirmation application research of 43 insertion/deletion and 1 amelogenin loci. The four different colors of fluorescent substances (FAM, HEX, TAMRA and ROX) are used for carrying out fluorescence labeling grouping on the primers of 44 loci, so that the 44 loci can be simultaneously subjected to composite amplification in the same amplification system. The InDels loci provided by the invention can be used for typing detection of degradation samples due to the fact that amplified fragments are short. The 44 loci contained in the system kithave high accumulated individual identification rate in irrelevant healthy individuals, and can meet the needs of individual identification of forensic degradation biological samples.

Description

technical field [0001] The invention belongs to the field of forensic new technology research, and specifically relates to the disclosure of a new 43 insertion / deletion (Insertion / Deletion, InDels) and a tooth enamel gene (Amelogenin ) site complex amplification typing detection kit. Background technique [0002] Short tandem repeat (STR) is a genetic polymorphism formed by repeating units composed of 2-6bp nucleic acid sequences; single nucleotide polymorphism (SNP) is caused by a single nucleotide variation at the genomic level DNA sequence polymorphism. SNP and STR are commonly used genetic markers in the field of forensic science because of their high polymorphism and widespread presence in the genome. However, most of the amplified fragments of STR markers are more than 300 bp, which is not conducive to the typing detection of degraded samples in forensic science. SNP markers are not conducive to the promotion in grass-roots forensic laboratories because of their comp...

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Application Information

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IPC IPC(8): C12Q1/6888
Inventor 朱波峰沈春梅解通方雅婷
Owner 朱波峰
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