9 results about "Amelogenin" patented technology

The invention discloses an enamel secretion prolonging and thickness increasing method based on KLK4 alpha protein, and relates to the technical field of biomedicine and dental materials.According to the method, expression of the KLK4 alpha protein is specifically regulated and controlled, forward intervention in the secretion period in the enamel development process is achieved, synthesis and accumulation of enamel matrix protein are directly promoted, and the effect of prolonging the secretion period of the enamel is achieved. A recombinant lentiviral vector mediated gene transduction technology is utilized to specifically introduce the KLK4alpha gene into a dental embryo glazing cell and realize overexpression, and the expressed KLK4alpha protein remarkably delays the process of transformation from a secretion period to a mature period by regulating the secretion activity of the glazing cell, so that the expression level of the KLK4alpha protein is improved, and the expression level of the KLK4alpha protein is improved. The time sequence intervention effectively prolongs the secretion window period of key organic matrix components such as glaze protein, glaze protein and the like, so that before mineralization is started, the total amount of extracellular matrix net racks is substantially increased, a space is provided for nucleation of hydroxyapatite crystals, and finally, the thickness of a mineral phase is remarkably increased; and the protection performance of the dental crown structure is enhanced.

The invention discloses a sequence optimization and affinity chromatography combined recombinant human glaze matrix protein efficient preparation method, and belongs to the technical field of bioengineering.The preparation method comprises the following steps that an expression strain is constructed, specifically, a human glaze matrix protein gene subjected to signal peptide removal and codon optimization is connected with an expression plasmid, a recombinant plasmid is constructed, and the expression strain is obtained; transforming to an expression host bacterium to obtain a recombinant strain; inducing expression; carrying out affinity chromatography purification; performing enzyme digestion to remove a label: cutting the primarily purified fusion protein by using specific protease to remove the label; and finely purifying. Through innovative combination of sequence optimization and a purification process, the problems that the recombinant glaze matrix protein is low in expression quantity, complex in purification process and high in cost are solved, and the prepared protein is high in purity and yield, has remarkable anti-periodontitis activity and is suitable for development of drugs, oral care products and skin care products.

This invention relates to the field of biomaterials technology, providing a composite material for biomimetic repair of dental hard tissues, its preparation method, and its application. The preparation method of the composite material includes the following steps: mixing a carboxybetaine-phosphonic acid copolymer with a calcium salt to obtain solution A; adding an aqueous phosphate solution to solution A to carry out an in-situ reaction to obtain the composite material for biomimetic repair of dental hard tissues. In the composite material provided by this application, the carboxybetaine-phosphonic acid copolymer, under the action of calcium phosphate ions, can form an amelogenin-like nanoribbon structure, simultaneously driving amorphous calcium phosphate to undergo supramolecular self-assembly, constructing a composite material conforming to the "self-assembly-mineralization" synergistic mode. This composite material can directionally deposit dense enamel-like enamel pillar structures of hydroxyapatite crystals on the surface of demineralized enamel, efficiently inducing oriented structural regeneration of damaged enamel and significantly restoring the mechanical properties of demineralized enamel.

The invention discloses a biomimetic mineralized hydrogel, which is characterized in that the biomimetic mineralized hydrogel is prepared from a cross-linked hydrogel matrix, mesoporous bioactive glass and an active polypeptide P148, wherein the cross-linked hydrogel matrix is cross-linked methylacryloylated gelatin, and the cross-linked hydrogel matrix is cross-linked methylacryloylated gelatin; the mesoporous bioactive glass is uniformly distributed in the cross-linked hydrogel matrix, and the active polypeptide P148 is loaded on the mesoporous bioactive glass. The invention further provides a preparation method of the biomimetic mineralized hydrogel and application of the biomimetic mineralized hydrogel in preparation of bone defect repair materials. According to the biomimetic mineralized hydrogel disclosed by the invention, a natural bone matrix is synergistically simulated from chemical components and a multilevel structure, a dual-action mechanism of mineralization induction and cell differentiation is realized, and an efficient and ordered bone regeneration solution is provided for critical-size bone defects.

The application discloses a kind of sequence optimization combined with high-efficiency preparation method of recombinant human enamel matrix protein of affinity chromatography, belong to the field of bioengineering technology, and preparation method includes the following steps: construction expression strain: remove signal peptide and the human enamel matrix protein gene of codon optimization is connected with expression plasmid, constructs recombinant plasmid, and is transformed into expression host bacteria, obtains recombinant strain;Induction expression;Affinity chromatography purification;Tag is removed by enzyme cutting: the fusion protein of preliminary purification is cut using specific protease to remove the label;Refine pure.The application solves the problems of low expression level of recombinant enamel matrix protein, complex purification process and high cost by the innovation of sequence optimization and purification process, the prepared protein is high in purity, high in yield, and has significant anti-periodontitis activity, and is suitable for the development of drugs, oral care products and skin care products.

The invention provides a composition for repairing tooth enamel damage and a preparation method and application thereof, and the composition for repairing tooth enamel damage comprises the following raw materials: glaze protein, procyanidine, a calcium ion source, a phosphate ion source and an orally acceptable carrier. According to the composition provided by the invention, by combining the glaze protein, the procyanidine and a biological remineralization technology, mineralized crystals can be induced to directionally grow along the damaged hydroxyapatite surface, a firmer structure is formed, remineralization is sustainable, and enamel is helped to be better repaired.

This invention discloses an oral homeostasis regulating mouthwash based on co-loaded active peptides and human recombinant enamel matrix protein. The mouthwash comprises the following components: active peptides, human recombinant enamel matrix protein, encapsulating material, pH buffer, and the remainder being deionized water. This invention is the first to combine two active ingredients with different physicochemical properties to form a stable and homogeneous delivery system. This design not only solves the problems of poor solubility of enamel matrix protein and instability caused by charge interaction when the two are directly mixed, but also improves the stability of the two active ingredients in the oral environment. While promoting nitric oxide production, regulating bacterial and immune balance, it also promotes periodontal tissue regeneration and repair. The two components synergistically restore oral homeostasis from both regulatory and repair dimensions.

The present application relates to the technical field of dental care, and particularly relates to a composition for guiding the generation of enamel-like hydroxyapatite and application and a method for generating hydroxyapatite. The composition of the present application comprises composition 1 and composition 2; the composition 1 takes Tris-HCl solution as a solvent and comprises the following components with final concentrations: 0.1-0.3 mg / ml of amelogenin, 0.1-0.3 mg / ml of amelotin C-terminal peptide; the composition 2 takes Tris-HCl solution as a solvent and comprises the following components with final concentrations: 0.1-0.3 mg / ml of amelogenin, 0.1-0.3 mg / ml of enamel maturation protein. The composition of the present application can realize the biomimetic remineralization of micron-level demineralized enamel and form new hydroxyapatite which is close to the structure of enamel and has no obvious interface.