Kit for 35 insertion/deletion sites in forensic individual identification

Abstract

The invention belongs to the field of new research of forensic medicine, and particularly relates to a composite amplification typing detection system kit and a detection method for 35 new insertion/deletion (InDel) sites in forensic individual identification research. According to the present invention, the primers of InDel sites are respectively subjected to fluorescent labeling with four fluorescent substances (FAM, HEX, TAMRA and ROX), such that the 35 sites are subjected to composite amplification in the same amplification system, and are detected synchronously; the InDel sites are the length polymorphism molecular genetic markers, can be typed by using the capillary electrophoresis method, and are easily applied and promoted in the basic forensic department; the kit is compatible with various biological samples, and has advantages of high sensitivity, strong specificity and simple and quick operation; and the 35 InDel sites have high cumulative individual recognition rate in unrelated healthy individuals, can meet the needs of forensic individual identification, and further can provide new and effective detection reagents for forensic practice.

Description

technical field [0001] The invention belongs to the field of forensic new technology research, and specifically relates to the disclosure of a new compound amplification typing detection kit for 35 insertion / deletion (Insertion / Deletion, InDel) sites suitable for forensic individual identification research. Background technique [0002] Short tandem repeats are genetic polymorphisms formed by repeating units composed of 2-6bp nucleic acid sequences. Because of their high polymorphism advantages, they are widely used in forensic research. However, most of the STR amplified fragments are above 300 bp, which is not conducive to the typing and detection of degraded samples in forensic science. [0003] Insertion / deletion polymorphic sites are genetic polymorphic sites formed by the insertion or deletion of DNA fragments of different lengths in the genome. InDel sites are widely distributed in the human genome, with an average of 7.2kp containing one InDel site. InDel sites hav...

AI Technical Summary

Problems solved by technology

[0004] Investigator, the most commonly used InDel typing detection kit in the world ® DIPplex is mainly developed for European and American populations. The polymorphism of these sites is low in the domestic population, and most of the sites have low application value in the domestic population.

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