Cultivation method of dendrobium officinale group
A cultivation method and technology of Dendrobium candidum, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow growth cycle, extinction of wild Dendrobium candidum, difficulty in survival, etc., and achieve fast multiplication speed, good flatness, The effect of low mutation rate
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[0036] Seed selection began in August 2013, followed by cultivation;
[0037] 1) reproduce,
[0038] a. Protocorm induction
[0039] At the beginning of September 2013, wash the surface of the collected mature seeds with detergent, rinse them under tap water for 10 minutes, then disinfect them with 75% alcohol for 30 seconds, wash them three times with sterile water, and then disinfect them with 0.1% mercuric chloride 10 min, then washed 4 times with sterile water; prepare a mixture of MS, sugar 30g / L, carrageenan 8.6 g / L, and 2g / L coconut milk as the seeding medium, and then incubate the seeds in the medium for 7 days in the dark. When the seeds start to germinate and the color is a little whitish, put the germinated seeds under the light and cultivate them; after 20 days, take the protocorms and prepare a mixture of 1 / 2MS+6-BA 2.2 mg / L+NAA as the proliferation medium, and take out The protocorms were inoculated into the proliferation medium and cultivated for 30 days;
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