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X-STR locus fluorescence labeling composite amplification system and application thereof

A composite amplification system and fluorescent labeling technology, which is applied in the direction of fluorescence/phosphorescence, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problem of limiting the application of X-chromosomal genetic markers, the difficulty of increasing genetic markers, and the high price of kits and other problems, to achieve the effect of low mutation rate, high sensitivity, and accurate typing results

Inactive Publication Date: 2010-07-28
SUN YAT SEN UNIV
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Problems solved by technology

[0009] (1) Difficulties encountered when using such kits to increase genetic markers;
[0010] (2) These X-STR gene loci were developed based on data from groups other than the Chinese population (mainly the white population), and some of these gene loci have poor gene frequency distribution in the Chinese population and low personal identification ability ;
[0011] (3) Foreign commercial kits are expensive
[0012] These shortcomings limit the application of X chromosome genetic markers in China, which is not conducive to further promotion at the grassroots level

Method used

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  • X-STR locus fluorescence labeling composite amplification system and application thereof
  • X-STR locus fluorescence labeling composite amplification system and application thereof
  • X-STR locus fluorescence labeling composite amplification system and application thereof

Examples

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Embodiment 1

[0056] Example 1 Using the X-STR locus fluorescent labeling multiplex amplification system containing 14 loci to identify sisters and identify grandmothers and granddaughters.

[0057] Principle: The X chromosome is the human sex chromosome, and X-STR is a type of polymorphic genetic marker on the sex chromosome. The X-STR of a male can only be obtained from his mother in the form of haplotype, and can only be passed on to his daughter. Therefore, sisters born to the same father have 1 identical allele at the X-STR locus. Same paternal origin cannot be ruled out if 1 identical allele at all X-STR loci is present in a sample of suspect sisters, but can be ruled out if no identical allele at 3 or more X-STR loci It is from the same father. Similarly, the granddaughter’s X-STR locus has 1 allele that is the same as her grandmother’s, so the suspected grandmother and granddaughter have 1 of the same alleles in all X-STR loci, and their genetic relationship cannot be ruled out. If...

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Abstract

The invention discloses an X-STR locus fluorescence labeling composite amplification system and an application thereof. The composite amplification system comprises 14 loca in total: DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, GATA31E08, DXS10074 and DXS10079. The 14 X-STR loca are amplified simultaneously in a composite amplification system in a fast and convenient manner; the PCR product can be standardized and internationalized and ensure relative accuracy of different lab data with genetic analyzer electrophoresis and automatic result analysis.

Description

technical field [0001] The invention relates to the detection of polymorphic genetic markers in the human genome, in particular to an X-chromosomal genetic marker technology, in particular to an X-STR gene locus fluorescent marker composite amplification system and its application. Background technique [0002] Short tandem repeats (short tandem repeats, referred to as STR) are a type of microsatellite DNA sequence formed by tandem repeats with 2 to 7 base pairs as the core unit, and its fragments can be amplified by PCR technology. STR is mainly due to the change of the number of core repeating units to form the genetic polymorphism of the STR gene locus, and its alleles can be typed by techniques such as silver staining, fluorescent labeling and autoradiography. In the human genome, there is an STR gene locus every 6-10kb on average, which provides a rich source of high-information gene loci for forensic personal identification and paternity testing. [0003] The X chromo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 刘秋玲赵虎吕德坚
Owner SUN YAT SEN UNIV
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