48results about How to "The typing result is accurate" patented technology

The invention belongs to the technical field of biological detection, and in particular relates to a fluorescently-labeled insertion/deletion (InDel) genetic polymorphism locus composite amplification system and application thereof. The application comprises the following steps of: selecting 35 InDel loci, designing polymerase chain reaction (PCR) amplification primers of the 35 InDel loci, matching fluorescein labels of the PCR primers, compositely amplifying and detecting the 35 InDel loci, and the like. The system can analyze 35 InDel genetic polymorphism loci by the composite amplification. The 35 InDel loci are labeled by FAM, HEX, TAMRA and ROX fluorescein with four different colors respectively. The composite amplification system based on the invention can be made into a kit, serves as an InDel locus fluorescence composite amplification kit with Chinese characteristics, and is applied to the fields, such as triplet paternity test, doublet paternity test, grandparent and grandchild test, sibling test, individual recognition, disease diagnosis, anthropology and the like.

The invention relates to a five-color fluorescence-labeling composite amplification system simultaneously analyzing 18 loci of human genomic DNA. The system divides the 18 loci into four groups, and relates to fluorescence labels in five colors in all. The fluorescence-labeling composite amplification system has the advantages of high sensitivity and capability of detecting the whole 18 loci under the condition that DNA template quantity is 0.2 ng. In Chinese Han population, the overall random matching probability is 8.8*10<-21>, and the cumulative non-parentage exclusion rate is 0.999999999993.

The invention discloses a relevant gene combination, a primer, a probe and application used for detecting a chemotherapeutic effect on acute myelogenous leukemia. The gene combination comprises four gene combinations closely relevant to pharmacotherapy of the acute myelogenous leukemia: medicament metabolism phase I enzyme gene CYP3A5, medicament metabolism phase II enzyme genes NAT2 and GSTO2, and medicament transport protein gene OATP1B1; and the gene combination comprises the following SNP loci: the locus rs776746 of the gene CYP3A5, the locus rs1799931 of the gene NAT2, the locus rs156697 of the gene GSTO2, and the locus rs4149056 of the gene OATP1B1. A method is performed by hybridizing a specific primer and probes which are subjected to fluorescence marks of different colors with target gene fragments in a nucleic acid sample after PCR amplification. The treatment effect of a leukemia medicament is detected and analyzed by mononucleotide extension technology so as to direct clinical medicament application, reduce a toxic or side effect of the medicament application for a patient, and increase a curative effect.

The invention relates to a composite amplification method and kit for STR loci. The composite amplification method and kit are used for amplifying 22 STR loci and one sex locus, wherein the 22 STR loci are composed of vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, D1S1656, CSF1PO, Penta D, Penta E, TH01, TPOX, D10S1248 and DYS391, and the sex locus is Amelogenin. Amplimers used in the composite amplification method and kit comprise sequences as shown in SEQ ID No. 1 to 46. The composite amplification method and kit provided by the invention can acquire information about the 22 STR loci with good polymorphism and high individual discrimination power and about the one sex locus through simultaneous amplification at a time; and a composite amplification system composed of the loci has good balance, high sensitivity and high specificity, is accurate in typing results and can totally meet demands of judicial expertise.

The invention discloses a human Y chromosome 37 STR loci fluorescence labelled multiplex amplification kit and an application thereof. The kit comprises a specific primer for amplifying 37 Y-STR lociwhich comprise 30 low-mutation Y-STR loci and 7 rapid-mutation Y-STR loci. The kit comprises 30 low-mutation and 7 rapid-mutation rate high-polymorphism Y-STR loci, considers distinguishing of checking of a male family and different male individuals of a same male line, and is the Y-STR detection product with a maximum loci quantity on market. The primer in the kit has the advantages of strong specification, high sensitivity, and accurate parting result, and practical case examination, DNA database construction and paternity identification requirements can be completely satisfied. The kit hasstrong check-material adaptability.

The invention discloses a high-throughput STR typing system capable of identifying complex consanguinity and a kit thereof, and relates to the technical field of nucleic acid detection in vitro. The high-throughput STR typing system capable of identifying complex consanguinity comprises PCR primers used for amplifying 60, 118 or 179 linked autosomal STR loci; and the kit comprises a PCR primer composition, an Index connector sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer solution B, a database setting-up reagent and the like. The high-throughput STRtyping system capable of identifying complex consanguinity and the kit thereof provided by the invention are capable of realizing single-tube amplification of 60, 118 or 179 linked autosomal STR loci; and moreover, the STR typing system and the kit thereof are good in balance, high in sensitivity, good in specificity, and accurate in typing result. Thus, the high-throughput STR typing system capable of identifying complex consanguinity and the kit thereof can be used for identifying complex relationships of different consanguinity at the same level.

The invention discloses a kit for synchronously detecting 124 micro haplotype gene loci on the basis of a next generation sequencing technology and a special primer pair combination thereof. The primer pair combination provided by the invention is composed of 248 molecules shown as sequences 1-248 in a sequence table. Experiments prove that the kit for synchronously detecting 124 micro haplotype gene loci on the basis of the next generation sequencing technology, provided by the invention, is used for detecting 124 micro haplotype gene loci of human genome DNA, and the typing result is accurate. The kit provided by the invention has a significant application value.

The invention discloses a reagent kit for detecting gene loci on the basis of next generation sequencing technologies and a special primer combination of the reagent kit. The primer combination comprises 60 types of DNA (deoxyribonucleic acid) molecules shown from sequences 1 to sequences 60 in sequence tables. The reagent kit for detecting the gene loci on the basis of the next generation sequencing technologies and the primer combination have the advantages that as proved by experiments, accurate typing results can be obtained when the reagent kit is used for detecting STR (short tandem repeat) typing of the 30 gene loci in standard substances 2800M; the reagent kit has important application value.

The invention provides a fluorescently-labeled X-InDel locus composite amplification system. By means of the system, eighteen insertion-deletion genetic polymorphism loci on an X chromosome can be compositely amplified, wherein the eighteen loci are respectively labeled with three fluoresceins FAM, HEX and TAMRA. The composite amplification system is high in sensitivity and individual identification rate, is high in polymorphism, is good in stability and repeatability, is accurate in typing results and can satisfy a practical requirement. A kit can be manufactured on the basis of the composite amplification system, can be used for paternity test, dyad paternity test, grandparent and grandchild test, sibling test and individual identification. New technologies can be provided for the fields such as anthropology, medical genetics and the like.

The invention relates to the technical field of genetic relationship identification methods, and discloses a genetic relationship identification method with SNP as a genetic marker. The method comprises the following three steps of: establishing a genetic relationship judgment model, carrying out typing detection on SNP sites, and confirming the genetic relationship among individuals according toa typing result and the judgment model. According to the genetic relationship identification method with the SNP as the genetic marker, the SNP is used as the genetic marker in replacement of STR in the detection mode, so the defects of poor stability, high typing difficulty, high sample quality requirements and high cost when the STR is used as the genetic marker for detection are fully avoided;whether two samples are of a direct line, or are in a first-level genetic relationship, a second-level genetic relationship or a third-level genetic relationship, or are strangers can be directly judged through a new model algorithm; a probability density model is introduced, so the influence caused by various errors is fully avoided; programmed judgment is adopted, so operation is easy and convenient; an SNP typing detection technology is adopted, so typing results are accurate and visual; and a great number of SNP sites are used by the algorithm, the number of repetitions is high, and the result is more accurate.

The invention discloses a new method for parting the single nucleotide polymorphisms (SNP) taking use of magnetic nanometer particle polymerase chain reaction (PCR), which is characterized in that a primer at a single side is fixed on magnetic nanometer particles and target sequence is expanded directly on the surfaces of magnetic nanometer particles, and then the sample is parted by hybridizing magnetic nanometer particles-ssDNA complex with allele specific probes. The method needs no purify and concentrate the product, the scans and parts rapidly and straightforwardly, is a parting method that is easy and rapid to operate, and is of high throughput and sensibility.

The invention belongs to the gene engineering technical field, and discloses a primer combination for detecting a short tandem repetitive sequence, a kit containing the primer combination and a method for detecting the short tandem repetitive sequence. Gene loci having high China people genetic polymorphism are selected and are more suitable for China people; the number of the selected tandem repetitive units is four, five or six, the slip peak ratio can be effectively reduced, and the accuracy of test results is higher; a fluorescent labeling composite amplification system is used for amplification, and amplified products can be subjected to electrophoresis in a same capillary, to achieve detection analysis of all the gene loci at the same time; a fluorescent label with mature technology is used for fluorescent labeling, and thus the cost is low; the detection method has good specificity and can be used for qualitative analysis such as individual identification, paternity identification, population genetic studies and the like, and can also be used for qualitative analysis of detection of the chimerism rate after hematopoietic stem cell transplantation.

The present invention discloses a multiple PCR targeted capture typing system and kit based on a high-throughput sequencing technology, and relates to the technical field of nucleic acid in-vitro detection. The STR typing system comprises PCR primers for amplification of 58, 119, or 179 linked autosomal STR gene loci. The kit comprises PCR primer combination, Index linker sequences, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer B, library building reagents, etc. The provided STR typing system and kit can achieve single-tube amplification of the 58, 119, or 179 linked autosomal STR gene loci, are good in balance, high in sensitivity, good in specificity, and accurate in typing results, and can be used to identify complex consanguinity relationships of different consanguinity relationships at a same level.

The invention claims a linked autosomal short tandem repeat (STR) typing system based on a high-throughput sequencing technology and a kit, and relates to the technical field of nucleic acid in-vitrodetection. The STR typing system includes PCR primers for amplification of 61, 121 or 179 linked autosomal STR loci, and the kit includes a PCR primer combination, an Index linker sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancing agent NB, a YF buffer liquid B and a library building reagent. The STR typing system and kit provided by the invention can realize single-tubeamplification of the 61, 121 or 179 linked autosomal STR loci, have good balance, high sensitivity, good specificity, and accurate typing results, and can be used to identify complex genetic relationships of different genetic relationships at a same level.

The invention discloses an X-STR locus fluorescence labeling composite amplification system and an application thereof. The composite amplification system comprises 14 loca in total: DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, GATA31E08, DXS10074 and DXS10079. The 14 X-STR loca are amplified simultaneously in a composite amplification system in a fast and convenient manner; the PCR product can be standardized and internationalized and ensure relative accuracy of different lab data with genetic analyzer electrophoresis and automatic result analysis.

The invention provides a method and a system for obtaining a DIP-STR (Deletion/Insertion Polymorphism-Short Tandem Repeat polymorphism) gene locus typing result of the DNA (deoxyribonucleic acid) of an unknown individual source in a mixed spot. The method comprises the following steps of: obtaining the mixed DNA in the mixed spot and the DNA of a single individual of which the individual source isknown; obtaining the mixed typing result of the mixed DNA in the mixed spot, and a typing result, i.e., a DNA typing result of the known individual source, of the DNA of the single individual of which the individual source is known; and according to the typing result, obtaining a typing result of the DNA of the unknown individual source in the mixed spot. The invention also provides a system forthe DIP-STR gene locus typing result of the DNA of the unknown individual source in the mixed spot. By use of the scheme of the invention, a specific DIP-STR gene locus typing result is comprehensively analyzed to effectively obtain the DNA typing result of the unknown individual source, and powerful technical support is provided for public security organizations to quickly determine criminal suspects.

The invention discloses a kit for detecting a Y-STR gene locus based on a next-generation sequencing technology and a special primer combination thereof. The primer combination provided by the invention is composed of 38 types of DNA (Deoxyribonucleic Acid) molecules shown as a sequence 1 to a sequence 38 in a sequence table. An experiment proves that the kit for detecting the Y-STR gene locus based on the next-generation sequencing technology, provided by the invention, is used for detecting STR typing of 19 Y-STR gene loci in a standard product 2800M and a typing result is accurate. The kitprovided by the invention has important application value.