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Multiple PCR targeted capture typing system and kit based on high-throughput sequencing technology

A system and gene locus technology, applied in the field of multiple PCR target capture sequencing compound typing system and its kit, can solve the problem of inability to distinguish complex genetic relationships

Active Publication Date: 2019-11-26
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most linked STR studies are X-STR and Y-STR genetic markers, which require special kinship to be applied; independently inherited autosomal STRs cannot distinguish complex kinship

Method used

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  • Multiple PCR targeted capture typing system and kit based on high-throughput sequencing technology
  • Multiple PCR targeted capture typing system and kit based on high-throughput sequencing technology
  • Multiple PCR targeted capture typing system and kit based on high-throughput sequencing technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1: A STR typing system and its kit for complex genetic relationship identification

[0082] 1.1 Composition and characteristics of autosomal-linked STR loci:

[0083] (1) The STR typing system in this embodiment comprises the following 60 STR loci:

[0084] D1S2131、D1S3721、D1S2130、D1S1600、D1S1653、D1S1660、D1S3732、D2S1364、D2S2734、D2S1396、D2S428、D2S435、D2S1387、D2S1792、D2S1399、D2S2959、D3S2431、D3S4547、D4S1643、D4S2408、D4S3326、D4S2368、D5S2845、D5S1473、D5S813、 D5S1716、D5S1459、D5S1487、D5S1466、D5S2496、D5S2501、D6S1019、D6S2417、D6S2412、D6S1284、D7S1820、D7S3050、D7S821、D8S594、D8S1468、D8S2322、D8S569、D8S1475、D9S746、D9S319、D9S2149、D11S2010、D11S1392、D12S376、D12S1052、 D13S317, D13S790, D14S1432, D14S1428, D15S644, D15S1507, D16S752, D16S485, D20S481 and D20S1151;

[0085] It has the following measurable properties:

[0086] 1) The repeating unit of the core region is tetranucleotide;

[0087] 2) The heterozygosity of each STR is greater than 0.6;

[0088] 3) Each group contains ...

Embodiment 2

[0108] Example 2: A STR typing system and its kit for complex genetic relationship identification

[0109] 2.1 Composition and characteristics of autosomal-linked STR loci:

[0110] (1) The STR typing system in this embodiment comprises the following 58 STR loci:

[0111]D1S3736、D1S1665、D1S2127、D1S2138、D1S1642、D1S518、D1S1604、D1S3729、D1S3727、D2S1336、D2S1779、D2S1771、D2S437、D2S2970、D2S2944、D2S1327、D3S2402、D3S1766、D4S2411、D4S3351、D4S243、D4S2426、D4S2373、D5S1490、D5S2856、 D5S2495、D5S2855、D5S1722、D5S1725、D6S1048、D6S1275、D7S796、D7S1799、D8S2324、D8S2330、D8S1144、D9S745、D9S301、D9S1124、D10S1246、D10S2485、D11S1983、D11S2363、D11S1368、D12S393、D12S1063、D13S1807、D13S1492、D14S738、D14S301、 D14S583, D15S816, D15S1514, D18S872, D18S972, D18S548, D20S1145, D20S477;

[0112] It has the following measurable properties:

[0113] 1) The repeating unit of the core region is tetranucleotide;

[0114] 2) The heterozygosity of each STR is greater than 0.6;

[0115] 3) Each group contains at least two STRs...

Embodiment 3

[0128] Example 3: A STR typing system and its kit for complex genetic relationship identification

[0129] 3.1 Composition and characteristics of autosomal-linked STR loci:

[0130] (1) The STR typing system in this embodiment comprises the following 61 STR loci:

[0131] D1S532、D1S1611、D1S3733、D1S533、D1S1614、D2S2977、D2S1374、D2S1394、D2S2966、D2S2969、D2S1371、D2S434、D2S1338、D3S4016、D3S2388、D4S1626、D4S1653、D5S2858、D5S2796、D5S1463、D5S815、D5S2499、D5S2498、D6S1043、D6S1274、 D6S1056、D6S1013、D6S1054、D7S820、D7S2205、D7S3071、D7S2845、D8S2326、D8S1464、D8S2320、D8S1470、D8S588、D8S1471、D9S2026、D9S747、D9S2128、D10S2469、D10S1238、D11S4464、D11S4958、D13S1818、D13S767、D14S615、D14S608、D14S597、 D14S302, D14S749, D16S767, D16S3393, D18S537, D18S875, D18S1367, D20S1152, D20S206, D20S607, D20S1146;

[0132] It has the following measurable properties:

[0133] 1) The repeating unit of the core region is tetranucleotide;

[0134] 2) The heterozygosity of each STR is greater than 0.6;

[0135] 3) Each group ...

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Abstract

The present invention discloses a multiple PCR targeted capture typing system and kit based on a high-throughput sequencing technology, and relates to the technical field of nucleic acid in-vitro detection. The STR typing system comprises PCR primers for amplification of 58, 119, or 179 linked autosomal STR gene loci. The kit comprises PCR primer combination, Index linker sequences, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer B, library building reagents, etc. The provided STR typing system and kit can achieve single-tube amplification of the 58, 119, or 179 linked autosomal STR gene loci, are good in balance, high in sensitivity, good in specificity, and accurate in typing results, and can be used to identify complex consanguinity relationships of different consanguinity relationships at a same level.

Description

technical field [0001] The invention relates to the technical field of forensic detection, in particular to a composite typing system of multiple PCR targeted capture sequencing based on high-throughput sequencing technology and a kit thereof. Background technique [0002] Short tandem repeat (short tandem repeat, STR) is a special sequence composed of 2-6bp repeating units in series. The number of STR loci is large and widely distributed, accounting for about 3% of the entire genome, and its polymorphism is high. It is mainly derived from the difference in the number of core sequence repeats among individuals, which follows the Mendelian law of inheritance in the genetic process. Therefore, STR amplification detection technology is widely used in forensic individual identification, kinship identification and population genetics research. The most commonly used STR typing techniques are fluorescent labeling multiplex amplification combined with capillary electrophoresis (ca...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6869
CPCC12Q1/6869C12Q1/6888C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2535/122C12Q2525/151
Inventor 严江伟范庆炜张更谦张君程凤王萌春李万婷石林玉张晓梦张家榕
Owner SHANXI MEDICAL UNIV
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