74results about How to "Short build cycle" patented technology

The invention provides a speech corpus generating device and method. The speech corpus generating device comprises a speech extracting device, a speech identifying device and a text marking device, wherein the speech extracting device is used for extracting speech data of a preset pronouncing person from collected data; the speech identifying device is used for identifying the speech data of the preset pronouncing person as a text; and the text marking device is used for marking the text. The invention also provides a speech synthesizing system and method. According to the speech corpus generating device and method provided by the invention, the speech corpus is generated by automatically collecting the data and automatically processing the data, so that a large amount of labor cost is saved. Besides, a construction period of the speech synthesizing system is shortened, the speech synthesizing system is conveniently updated and the personalized customization is realized.

The invention discloses a self-adaptive dynamic construction method of a WIFI indoor positioning system fingerprint database. The method comprises the following steps of: 1) determining an AP layout mode, and properly reducing the number of APs; 2) determining the size of a grid and positions of sample reference points; 3) according to a 'useless AP rejecting' rule, rejecting related data of useless APs; 4) adopting a 3[delta] rule to carry out gross error processing on the data, and lowering the integral fluctuation degree of the data; and 5) according to a time moving window method, regularly updating the fingerprint database, and automatically adapting to interference caused by environment changes. According to the invention, the reasonable AP layout and the suitable grid size are selected, the construction of the fingerprint database is accelerated, and the fingerprint information is more comprehensive and accurate; in addition, the useless APs are rejected, and the fingerprint database is regularly updated, so that the complexity of a positioning algorithm is lowered, and the responsiveness and the environment interference resistance are improved for the positioning of a positioning system.

The invention discloses a method for rapidly establishing three-dimensional fine thermos-hydraulic calculation models for primary side systems of large-scale natural circulation vapor generators in nuclear power plants under certain heat transfer tube blocking accident conditions. The method comprises the following steps of: 1, carrying out complete primary side hydraulic analysis on a vapor generator so as to analyze a flow distribution law at a tube plate; 2, selecting a tube blocking scheme according to a result of the last step; 3, determining a tube blocking share and a tube blocking position; 4, carrying out geometric simplification and overall geometric modeling on a plurality of heat transfer tubes in the real vapor generator; 5, dividing preliminary calculation nodes; 6, establishing a tube blocking equation to tracing marked control areas, and carrying out calculation domain grid marking on the control areas by adoption of a network marking method so as to realize a tube blocking function; and 7, carrying out blocking condition calculation by adoption of a preliminarily established model and analyzing the precision of the result, and if a requirement is satisfied, considering that the modeling is completed, and otherwise, returning to the step 5 to encrypt the calculation nodes until the model satisfies the calculation precision requirement.

The invention discloses a method and device for constructing a clock network. The method for constructing the clock network comprises the steps of obtaining position information of the tail end of the clock network and setting time sequence devices in a setting area. The tail end of the clock network is a wire or a point for setting the time sequence devices in the clock network. When the tail end of the clock network is the wire used for setting the time sequence devices, the tail end of the clock network is used as a symmetry axis to determine the setting area of the time sequence devices, and when the tail end of the clock network is the point used for setting the time sequence devices, the tail end of the clock network is used as a center to determine the setting area of the time sequence devices. By means of the method and device for constructing the clock network, the problem hat in the prior art, resource cost is high or design period is long when the clock network is constructed is solved, and therefore the effects of lowering resource cost for constructing the clock network and shortening constructing period are achieved.

The invention relates to a method for rapidly predicting 28-day colloidal mortar compression strength of cement. In the method, an idea of Netherlands weighted maturity (also known as C value maturity) is adopted; a C value of a foreign cement weighting coefficient is referred to; the temperature measuring process is simplified; a group of colloidal mortar test blocks are formed by utilizing the conventional JC / T 738-2004 cement rapid test equipment; the compression strength in different ages is tested in 48 hours under the condition of hydrothermal curing at the temperature of 55 DEG C; a relation of the weighted maturity and the strength is established; and the 28-day strength of the cement is predicted according to the relation. Research shows that the method for rapidly predicting the 28-day colloidal mortar compression strength of the cement is feasible and effective and has high prediction accuracy on the 28-day strength.

The invention relates to the technical field of organic solid waste processing, in particular to an organic solid waste film covering high-temperature aerobic fermentation method. The fermentation method is characterized in that the organic solid waste is pretreated; the pretreated material is piled in strip pile; the material strip pile is provided with a sensor and covered with a waterproof breathable film; a blower and an air-pipe under the material are used for supplying oxygen to the material; an intelligent ventilating control system is used for optimizing the fermentation process; after the material is fully decomposed, the waterproof breathable film on the material strip pile is removed, and then the material strip pile is transported into a clinker storing or processing area. Compared with the prior art, the fermentation method has short building period, few disposable investment, flexible construction, low operating cost, small odor influence, rain-proof, wind prevention and heat preservation effect and can protect the material from being affected by outside weathers.

The invention belongs to the technical field of telex flight control system tests, and especially relates to a displacement sensor simulation circuit. The displacement sensor simulation circuit of the invention can be divided into three parts which are an input signal processing circuit, a signal modulation circuit, and an output signal processing circuit. The simulation circuit receives simulation output signals, and decomposes the simulation output signals into two paths of direct current simulation signals which are corresponding to two secondary manipulated variables in terms of sizes. The two direct current simulation signals are modulated to two paths of alternating current simulation signals through the utilization of an amplitude modulation principle. A driving capability of the signals is raised through the utilization of a reactive circuit composed of an operational amplifier and a triode, and a transformer is driven to be an output, so that secondary output impedance characteristics of a linear displacement sensor are simulated. The displacement sensor simulation circuit of the invention is simple in design, low in cost, and can simulate the signals of the displacement sensor very well.

The invention discloses a high-throughput STR typing system capable of identifying complex consanguinity and a kit thereof, and relates to the technical field of nucleic acid detection in vitro. The high-throughput STR typing system capable of identifying complex consanguinity comprises PCR primers used for amplifying 60, 118 or 179 linked autosomal STR loci; and the kit comprises a PCR primer composition, an Index connector sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer solution B, a database setting-up reagent and the like. The high-throughput STRtyping system capable of identifying complex consanguinity and the kit thereof provided by the invention are capable of realizing single-tube amplification of 60, 118 or 179 linked autosomal STR loci; and moreover, the STR typing system and the kit thereof are good in balance, high in sensitivity, good in specificity, and accurate in typing result. Thus, the high-throughput STR typing system capable of identifying complex consanguinity and the kit thereof can be used for identifying complex relationships of different consanguinity at the same level.

The invention discloses a biomolecule competition analysis method which is characterized in that the competition analysis method is established by aptamer which serves as an affinity ligand, together with a marked target. The method includes the following steps: (1) coating the target aptamer; (2) combining with the marked target module through incubation; (3) adding a target sample for competition analysis; and (4) after washing, adding substrate for analysis. The method is particularly suitable for detecting small molecular antigens and haptens. The invention further aims to solve the technical problem in providing the application of the biomolecule competition analysis method. The biomolecule competition analysis method is widely applicable in the fields of in vivo and in vitro detection and analysis of morbid substances, experimental study of humoral biomolecules, food safety monitoring, biological terrorist prevention and monitoring and environmental monitoring.

The invention relates to a method for obtaining a lymphoma minipig disease model by knocking out P53 genes, and belongs to the field of medical animal disease models. The method mainly comprises the steps that TALEN target spots of the P53 genes are designed, P53 gene knockout TALEN plasmid pCAG-pP53-L and pCAG-pP53-R of the P53 genes are assembled, TALEN plasmid is transfected and screened to obtain a P53 gene knockout positive cell line, reconstructed embryos are constructed based on the somatic cell nuclear transplantation technology and transplanted into the body of a surrogate sow to continue to develop, and a piglet with the P53 genes knocked out is obtained; the function of the P53 genes is verified by detecting the expression level of RNA and protein of the P53 genes in tissue of the cloned piglet and downstream related genes, and lymphoma in the tissue of the piglet is identified through histopathology and an immunohistochemical method. The built lymphoma disease model has great significance in research and development of occurring, forming and medicine of human lymphoma, and the reliable model is provided for research and treatment of human lymphoma diseases.

The invention discloses a microRNA inhibitor, a microRNA inhibitor expression vector, a building method of the microRNA inhibitor expression vector and application of the microRNA inhibitor expression vector. The microRAN inhibitor is a dMAN (double mRNA adhesion nucleotide), and consists of three parts including a general straight chain complementary sequence, a general stem ring sequence and two miRNA combining region sequences. The built expression vector comprises two dMAN expression frameworks; two identical or different MANs can be transcribed at the same time; the utilization rate of the vector is higher; and the consumption and the toxicity of virus particles are lower. In an aspect of the building method, the dMAN building method is economic; only two pairs of primers of at most 60 basic groups need to be synthesized; and one-step cloning building comprising two MAN structure mRNA inhibition vectors can be completed through twice PCR (Polymerase Chain Reaction) and an enzyme digestion and connecting technology.

The invention provides video monitoring system and method for traffic road conditions. The method comprises the following steps of: transmitting traffic video data acquired by a traffic video acquisition device to a stream media server in real time; then, circularly extracting video data frames from the stream media server through an image inversion server, and processing the video data frames into pictures through decoding and format conversion; and circularly acquiring corresponding pictures from the image inversion server by a WAP (Wireless Application Protocol) server according to a chaining request of a mobile terminal, forming a WAP standard formatted file, and sending the file to the mobile terminal with a WAP browser for displaying. By the measurements of the invention, the network transmission problem of monitoring images, the limitation problem of the processing performance of the mobile terminal and the problem of difference formats of files supported by different mobile phones are solved, so that the monitoring of the traffic road conditions in the traditional communication environment can be realized and popularized on the mobile terminal, and the operation of the mobile terminal does not influence the independent operation of the existing stream media server.

The invention discloses an artificial algae field. The artificial algae field is characterized by comprising a plurality of algal reefs which are connected together by a fixed rope, wherein a seedling hanging net is fixed between the algal reefs; a grown algae is suspended on the seedling hanging net; algae seedlings are attached to the algal reefs. By adopting a form of combining the algal reefs with the seedling hanging net, the algae seedlings and the grown algae are simultaneously led to the seafloor; the leading-in effect of the algae is ensured; the building cycle of the algae field is shortened. The required building material is cheap and available; the seedlings are suspended by a polythene net, so that the density of the led-in algae is greatly improved; the capability of tolerating biological food pollution is improved; the algal reefs are orderly connected together by using the fixed rope; the capability of withstanding stormy waves is improved; the algal reefs adopt the form of combining mother reefs with son reefs, so that the artificial algae field is convenient to transport, the work amount is reduced, and the success rate of building the algae field is greatly improved.

The invention discloses an ietalurus punetaus half-sib family large-scale building method. The method comprises the steps that 1, parents are bred; 2, a paternal line half-sib family is built; 3, mating and oviposition are conducted; 4, egg masses are incubated, wherein PIT electronic markers are injected into females laying eggs; 5, artificial spawning is conducted, wherein the females are put into an ovipositor to mate with males after injection; 6, after oviposition is completed, the egg masses are taken out for subsequent incubation, and meanwhile the females and the males who lay the eggs are taken out, injected with the PIT electronic markers and put into a pond to continue to be bred, and serve as reproduction of the subsequent year. By means of the method, the ietalurus punetaus half-sib family large-scale building success rate is significantly increased, and the family building cycle is shortened.

The invention discloses a method for preparing animal cell galactosylated modification influenza HA (hemagglutinin) glycoprotein from glycosyl engineering yeast. The method provided by the invention comprises the following steps of expressing influenza virus HA genes in a yeast mutant to obtain recombinant yeast; and culturing the recombinant yeast for preparation to obtain an influenza virus HA glycoprotein with mammal glycoform structures but without fucose. Experiments prove that a vaccine prepared from influenza HA glycoprotein particles prepared by the method can be induced to generate high anti-influenza-virus neutralizing antibodies, and the problems of allergy and the like possibly caused by fungus galactosylated modification can be solved.

The invention relates to a minicircle DNA carrier expressing a target cell-effector cell bridge, a preparation method and application thereof, in particular to a minicircle DNA carrier for in vivo expression of a target cell-effector cell bridge. The target cell-effector cell bridge can specifically combine target cell effect targets and effector cell effect targets, thereby effectively connectingtarget cell to effector cell, and mediating killing to target cell by effector cell. The target cell-effector cell bridge can be used for treating cancers, infections or other diseases.

The invention discloses a correction method considering the influence of a local structure in reactor core flow field calculation, which can add the influence of a positioning grid on a local flow field to overall flow field calculation for correction when performing three-dimensional thermal hydraulic calculation on a fuel assembly with the positioning grid. The correction process comprises the following steps: 1, determining a correction area; 2, establishing a calculation region geometric model; 3, performing hydraulics analysis of a local flow field; 4, calculating an influence range and an influence factor; and 5, substituting into overall calculation for correction. According to the method, the defect that an existing reactor core flow field three-dimensional analysis program difficultly reflects a local flow field near the positioning grillwork can be overcome, and the accuracy of a result obtained through numerical calculation is improved; the successful establishment of the local flow field correction method can be suitable for various computational fluid mechanics analysis programs to carry out numerical simulation on various rod bundle / tube bundle channels containing similar structures under different working conditions to obtain a calculation result capable of reflecting a local flow field near a local structure; and the correction method is also suitable for similar working conditions of other similar equipment.

The invention belongs to the technical field of elegans gene editing, and more specifically discloses a method for rapidly constructing target pU6-sgRNA plasmids required for caenorhabditis elegans gene editing. pU6-2X BseRI-sgRNA plasmids are constructed by modifying an original carrier for caenorhabditis elegans pU6-unc-119sgRNA, the pU6-2X BseRI-sgRNA plasmids are digested by using BseRI enzymeto obtain a carrier with cohesive ends, sgRNA target DNA small fragments with cohesive ends are formed by positive and negative primers containing sgRNA target sequences and cohesive ends through annealing, under the effect of T4DNA ligase, the carrier is connected with the sgRNA target DNA small fragments with the mated cohesive ends, DH5 alpha competent cells are converted, positive monoclonesare screened from escherichia coli after conversion, and whether an sgRNA target sequence is correct is verified through sequencing. By using the technical scheme, the construction period is less thanone day, the operation before T4DNA ligase consumes less than half an hour and is reduced by at least four hours compared with pure manpower consumed time in a conventional method, and the construction period is reduced by two days. The sgRNA plasmid is constructed as long as only two primers containing 44 basic groups need to be synthesized, so that the construction cost is greatly reduced.

The invention discloses a construction method for a nasopharynx cancer clinic related radioresistance cell strain. The method comprises the following steps of culturing a CNE-2 cell, and constructinga CNE-2R cell strain, wherein according to the irradiation mode, irradiation is completed according to the gradient irradiation mode that 0.5Gy*5 times, 1.0Gy*5 times, 1.5Gy*5 times, 2.0Gy*30times andaccumulated 75Gy. According to the construction method for the nasopharynx cancer clinic related radioresistance cell strain, the method adopts irradiation with the partition dosage being not largerthan 2.0Gy, is close to clinic practice, and can better reflect the actual clinic condition; compared with conventional, repeated and other irradiation modes, the method adopts the gradient irradiation mode, the total irradiation dosage, irradiation frequency and construction period are better balanced, and the construction period is short.

The invention relates to the field of gene mutation detection, in particular to a detection kit for NK / T cell lymphoma related genes and a library building method. The detection kit for the NK / T celllymphoma related genes comprises a capture probes for 65 target genes. The kit adopts two rounds of PCR reactions for library building, and the library building has a short period, a high coverage rate, a high comparison rate, a good uniformity, simple operations and the like.

The present invention discloses a multiple PCR targeted capture typing system and kit based on a high-throughput sequencing technology, and relates to the technical field of nucleic acid in-vitro detection. The STR typing system comprises PCR primers for amplification of 58, 119, or 179 linked autosomal STR gene loci. The kit comprises PCR primer combination, Index linker sequences, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer B, library building reagents, etc. The provided STR typing system and kit can achieve single-tube amplification of the 58, 119, or 179 linked autosomal STR gene loci, are good in balance, high in sensitivity, good in specificity, and accurate in typing results, and can be used to identify complex consanguinity relationships of different consanguinity relationships at a same level.

The invention claims a linked autosomal short tandem repeat (STR) typing system based on a high-throughput sequencing technology and a kit, and relates to the technical field of nucleic acid in-vitrodetection. The STR typing system includes PCR primers for amplification of 61, 121 or 179 linked autosomal STR loci, and the kit includes a PCR primer combination, an Index linker sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancing agent NB, a YF buffer liquid B and a library building reagent. The STR typing system and kit provided by the invention can realize single-tubeamplification of the 61, 121 or 179 linked autosomal STR loci, have good balance, high sensitivity, good specificity, and accurate typing results, and can be used to identify complex genetic relationships of different genetic relationships at a same level.

The invention relates to molecular biology, specifically to a genetic element applicable to genetic modification of thermophilic microorganisms, a carrier and application thereof. The element is a thermophilic type-II intron element TeI3c-4c, shown by a nucleotide sequence in sequence tables SEQ ID NO.1 and 2. A targeting vector of the genetic element for genetic modification of the thermophilic microorganisms is applied to the thermophilic microorganisms, thereby subjecting the thermophilic microorganisms to gene targeting inactivation. According to the invention, Thermotargetron is used and applied to a type-II intron-based genetic inactivation tool for the thermophilic microorganisms. The advantages are that the genetic inactivation efficiency is high; the targeting point can be easily changed (the targeting carrier establishment period is short); the host range is wide (applicability to Gram positive and negative bacteria); no nutritional deficiency selection marker is needed; the requirement on conversion efficiency is low; and inactivation of relatively-short (100-200bp) gene sequences can be performed.

The invention discloses a graph Web display method based on a panorama for an industrial monitoring system. The method includes the steps of using a panorama scene editor to import a panorama resource set and edict and generate a panorama monitoring engineering file set; exporting the panorama monitoring engineering file set as a Web item file set to be imported into a Web server for conducting Web display. According to the method, construction is conducted on the basis of the panorama; the graph Web display method has the advantages of being not only short in development period of two-dimensional graphs and low in cost, but great in universality and interactivity of three-dimensional models, and meanwhile, real scenes can be restored to the maximum degree, and the method can cooperate with the existing industrial monitoring systems for usage to meet the demands for display and interactivity in complex scenes.

The invention relates to a banking industry-oriented full-stack financial knowledge graph platform, which comprises a graph construction layer configured to perform source data management, logic graph ontology creation, graph production, graph treatment and task scheduling; the map warehouse management layer is configured to store maps of different fields for specific business scenes; the service application layer is configured to perform map visual search, map data mining, map query and map model application; and the application interface layer is configured to provide financial knowledge graph service access capability for the platform. Compared with the prior art, the method has the advantages that key graph management links of knowledge acquisition, knowledge representation, knowledge storage, knowledge fusion, knowledge modeling, knowledge calculation and knowledge application are realized, the whole process from knowledge raw materials to intelligent data conversion is supported, full-stack graph production and application service capability is provided, and graph construction and production efficiency is improved.

The invention provides a construction method of a high-yield valarene gene engineering bacterium, which comprises the following steps: integrating and expressing an MVA pathway rate-limiting enzyme-truncated HMG-CoA reductase coding gene (tHMG1) and integrating and fusing and expressing an FPP synthase coding gene (ERG20) and a valarene synthase coding gene (SmVS) in saccharomyces cerevisiae in a homologous recombination manner, so as to enhance the metabolic intensity of an MVA pathway; and the expression of the varrenene is enhanced. Meanwhile, a squalene synthase encoding gene ERG9 promoter is replaced with a copper ion induced promoter pCUP1, the ergosterol competitive pathway is reduced, and the yield of the valarene is increased; and finally, obtaining the gene engineering strain with high yield of the varrenene. The shake flask fermentation yield of the valarene of the genetically engineered bacterium can reach about 117mg / L, the fermentation tank yield can reach about 8g / L, and the genetically engineered bacterium completely has a commercial production level and has a good industrial application prospect.

The invention discloses an establishing method of an osteosarcoma EPI (Epirubicin) drug-resistant cell line. The establishing method comprises the following step: by taking human 143b cells as parentcells and EPI as an induction drug, jointly implementing a drug gradual increment induction method and a large dosage impact induction method with in a drug concentration range of 3 to 120 nM / L untilobtaining a drug-resistant cell line which can be in normal growth and passage under the drug concentration of at least 120 nM / L. The establishing method disclosed by the invention has the characteristics of short establishing period and stable drug resistance of the drug-resistant cell line, and the established drug-resistant cell line can be used for research on an osteosarcoma EPI drug-resistant mechanism and research on reversing drug resistance.

The invention discloses a genetic engineering bacterium producing beta-ionone with high yield and a construction method and application of the genetic engineering bacterium, and belongs to the field of microbial fermentation. In the method, by strengthening MVA pathway and introducing phosphotransketase pathway, the supply of acetyl-CoA in cytoplasm and the metabolic flux of terpenoid synthesis pathway are increased, so that the genetic engineering bacterium producing beta-ionone with high yield is successfully constructed and obtained. The genetic engineering bacteria obtained according to the method of the invention can produce beta-ionone under conventional fermentation conditions, the fermentation yield in a shake flask can reach about 358 mg / L, and the fermentation yield in a 3L fermentor can reach about 0.98 g / L, which is close to an industrialization level. According to the present invention, the perfume beta-ionone can be produced by utilizing a simple medium for fermentation,one-time and highly efficient transformation and integration of multiple genes can be realized, the construction time of the engineering bacteria can be significantly shortened, the perfume beta-ionone can be produced by the obtained engineering bacteria with simple carbon sources such as glucose and glycerin for fermentation, and the engineering bacteria has good industrial application prospects.

The invention relates to the technical field of surveying and mapping, and discloses a method and a device for rapidly establishing a national coordinate framework reference. The method comprises thefollowing steps of carrying out GNSS data observation and acquisition on the reference point to be solved on an operation area, calculating the current epoch coordinates of the datum points to be solved under an ITRF framework; acquiring the velocity field information of a landmark network reference station; establishing a speed field model in the national range according to the speed field information of the reference station; calculating the speed of the reference point to be solved according to the speed field model; carrying out framework conversion according to the speed of the referencepoint to be solved, and converting the current epoch coordinate of the reference point to be solved under the ITRF framework into a CGCS2000 framework coordinate. A national high-level control point is not needed to rapidly establish the national CGCS2000 coordinate framework reference in the national range.

The invention discloses a preparation method of a fusion mutant of Ebola virus glycoprotein and matrix protein by saccharomycetes. The preparation method includes steps of 1), introducing gene for encoding the fusion mutant of Ebola virus glycoprotein and matrix protein to a receptor yeast cell to obtain the recombined yeast cell for expressing the gene; 2), orderly cultivating and breaking the recombined yeast cell to obtain the fusion mutant of Ebola virus glycoprotein and matrix protein from the broken product. The fusion mutant of Ebola virus glycoprotein and matrix protein is a recombined protein obtained by fusing an Ebola virus glycoprotein core zone reserved with an Ebola virus glycoprotein receptor bond zone and a glycan cap zone to an N terminal of the Ebola virus matrix protein. The fusion mutant of Ebola virus glycoprotein and matrix protein has a polymer structure and has galactosylated modification; therefore, the fusion mutant is potential to be a vaccine for preventing Ebola hemorrhagic fever.