73results about How to "Short build cycle" patented technology

The invention provides a speech corpus generating device and method. The speech corpus generating device comprises a speech extracting device, a speech identifying device and a text marking device, wherein the speech extracting device is used for extracting speech data of a preset pronouncing person from collected data; the speech identifying device is used for identifying the speech data of the preset pronouncing person as a text; and the text marking device is used for marking the text. The invention also provides a speech synthesizing system and method. According to the speech corpus generating device and method provided by the invention, the speech corpus is generated by automatically collecting the data and automatically processing the data, so that a large amount of labor cost is saved. Besides, a construction period of the speech synthesizing system is shortened, the speech synthesizing system is conveniently updated and the personalized customization is realized.

The invention discloses a method for rapidly establishing three-dimensional fine thermos-hydraulic calculation models for primary side systems of large-scale natural circulation vapor generators in nuclear power plants under certain heat transfer tube blocking accident conditions. The method comprises the following steps of: 1, carrying out complete primary side hydraulic analysis on a vapor generator so as to analyze a flow distribution law at a tube plate; 2, selecting a tube blocking scheme according to a result of the last step; 3, determining a tube blocking share and a tube blocking position; 4, carrying out geometric simplification and overall geometric modeling on a plurality of heat transfer tubes in the real vapor generator; 5, dividing preliminary calculation nodes; 6, establishing a tube blocking equation to tracing marked control areas, and carrying out calculation domain grid marking on the control areas by adoption of a network marking method so as to realize a tube blocking function; and 7, carrying out blocking condition calculation by adoption of a preliminarily established model and analyzing the precision of the result, and if a requirement is satisfied, considering that the modeling is completed, and otherwise, returning to the step 5 to encrypt the calculation nodes until the model satisfies the calculation precision requirement.

The invention relates to a method for rapidly predicting 28-day colloidal mortar compression strength of cement. In the method, an idea of Netherlands weighted maturity (also known as C value maturity) is adopted; a C value of a foreign cement weighting coefficient is referred to; the temperature measuring process is simplified; a group of colloidal mortar test blocks are formed by utilizing the conventional JC/T 738-2004 cement rapid test equipment; the compression strength in different ages is tested in 48 hours under the condition of hydrothermal curing at the temperature of 55 DEG C; a relation of the weighted maturity and the strength is established; and the 28-day strength of the cement is predicted according to the relation. Research shows that the method for rapidly predicting the 28-day colloidal mortar compression strength of the cement is feasible and effective and has high prediction accuracy on the 28-day strength.

The invention relates to the technical field of organic solid waste processing, in particular to an organic solid waste film covering high-temperature aerobic fermentation method. The fermentation method is characterized in that the organic solid waste is pretreated; the pretreated material is piled in strip pile; the material strip pile is provided with a sensor and covered with a waterproof breathable film; a blower and an air-pipe under the material are used for supplying oxygen to the material; an intelligent ventilating control system is used for optimizing the fermentation process; after the material is fully decomposed, the waterproof breathable film on the material strip pile is removed, and then the material strip pile is transported into a clinker storing or processing area. Compared with the prior art, the fermentation method has short building period, few disposable investment, flexible construction, low operating cost, small odor influence, rain-proof, wind prevention and heat preservation effect and can protect the material from being affected by outside weathers.

The invention discloses a high-throughput STR typing system capable of identifying complex consanguinity and a kit thereof, and relates to the technical field of nucleic acid detection in vitro. The high-throughput STR typing system capable of identifying complex consanguinity comprises PCR primers used for amplifying 60, 118 or 179 linked autosomal STR loci; and the kit comprises a PCR primer composition, an Index connector sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer solution B, a database setting-up reagent and the like. The high-throughput STRtyping system capable of identifying complex consanguinity and the kit thereof provided by the invention are capable of realizing single-tube amplification of 60, 118 or 179 linked autosomal STR loci; and moreover, the STR typing system and the kit thereof are good in balance, high in sensitivity, good in specificity, and accurate in typing result. Thus, the high-throughput STR typing system capable of identifying complex consanguinity and the kit thereof can be used for identifying complex relationships of different consanguinity at the same level.

The invention relates to a method for obtaining a lymphoma minipig disease model by knocking out P53 genes, and belongs to the field of medical animal disease models. The method mainly comprises the steps that TALEN target spots of the P53 genes are designed, P53 gene knockout TALEN plasmid pCAG-pP53-L and pCAG-pP53-R of the P53 genes are assembled, TALEN plasmid is transfected and screened to obtain a P53 gene knockout positive cell line, reconstructed embryos are constructed based on the somatic cell nuclear transplantation technology and transplanted into the body of a surrogate sow to continue to develop, and a piglet with the P53 genes knocked out is obtained; the function of the P53 genes is verified by detecting the expression level of RNA and protein of the P53 genes in tissue of the cloned piglet and downstream related genes, and lymphoma in the tissue of the piglet is identified through histopathology and an immunohistochemical method. The built lymphoma disease model has great significance in research and development of occurring, forming and medicine of human lymphoma, and the reliable model is provided for research and treatment of human lymphoma diseases.

The invention discloses a microRNA inhibitor, a microRNA inhibitor expression vector, a building method of the microRNA inhibitor expression vector and application of the microRNA inhibitor expression vector. The microRAN inhibitor is a dMAN (double mRNA adhesion nucleotide), and consists of three parts including a general straight chain complementary sequence, a general stem ring sequence and two miRNA combining region sequences. The built expression vector comprises two dMAN expression frameworks; two identical or different MANs can be transcribed at the same time; the utilization rate of the vector is higher; and the consumption and the toxicity of virus particles are lower. In an aspect of the building method, the dMAN building method is economic; only two pairs of primers of at most 60 basic groups need to be synthesized; and one-step cloning building comprising two MAN structure mRNA inhibition vectors can be completed through twice PCR (Polymerase Chain Reaction) and an enzyme digestion and connecting technology.

The invention discloses an artificial algae field. The artificial algae field is characterized by comprising a plurality of algal reefs which are connected together by a fixed rope, wherein a seedling hanging net is fixed between the algal reefs; a grown algae is suspended on the seedling hanging net; algae seedlings are attached to the algal reefs. By adopting a form of combining the algal reefs with the seedling hanging net, the algae seedlings and the grown algae are simultaneously led to the seafloor; the leading-in effect of the algae is ensured; the building cycle of the algae field is shortened. The required building material is cheap and available; the seedlings are suspended by a polythene net, so that the density of the led-in algae is greatly improved; the capability of tolerating biological food pollution is improved; the algal reefs are orderly connected together by using the fixed rope; the capability of withstanding stormy waves is improved; the algal reefs adopt the form of combining mother reefs with son reefs, so that the artificial algae field is convenient to transport, the work amount is reduced, and the success rate of building the algae field is greatly improved.

The invention discloses a method for preparing animal cell galactosylated modification influenza HA (hemagglutinin) glycoprotein from glycosyl engineering yeast. The method provided by the invention comprises the following steps of expressing influenza virus HA genes in a yeast mutant to obtain recombinant yeast; and culturing the recombinant yeast for preparation to obtain an influenza virus HA glycoprotein with mammal glycoform structures but without fucose. Experiments prove that a vaccine prepared from influenza HA glycoprotein particles prepared by the method can be induced to generate high anti-influenza-virus neutralizing antibodies, and the problems of allergy and the like possibly caused by fungus galactosylated modification can be solved.

The invention belongs to the technical field of elegans gene editing, and more specifically discloses a method for rapidly constructing target pU6-sgRNA plasmids required for caenorhabditis elegans gene editing. pU6-2X BseRI-sgRNA plasmids are constructed by modifying an original carrier for caenorhabditis elegans pU6-unc-119sgRNA, the pU6-2X BseRI-sgRNA plasmids are digested by using BseRI enzymeto obtain a carrier with cohesive ends, sgRNA target DNA small fragments with cohesive ends are formed by positive and negative primers containing sgRNA target sequences and cohesive ends through annealing, under the effect of T4DNA ligase, the carrier is connected with the sgRNA target DNA small fragments with the mated cohesive ends, DH5 alpha competent cells are converted, positive monoclonesare screened from escherichia coli after conversion, and whether an sgRNA target sequence is correct is verified through sequencing. By using the technical scheme, the construction period is less thanone day, the operation before T4DNA ligase consumes less than half an hour and is reduced by at least four hours compared with pure manpower consumed time in a conventional method, and the construction period is reduced by two days. The sgRNA plasmid is constructed as long as only two primers containing 44 basic groups need to be synthesized, so that the construction cost is greatly reduced.

The present invention discloses a multiple PCR targeted capture typing system and kit based on a high-throughput sequencing technology, and relates to the technical field of nucleic acid in-vitro detection. The STR typing system comprises PCR primers for amplification of 58, 119, or 179 linked autosomal STR gene loci. The kit comprises PCR primer combination, Index linker sequences, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer B, library building reagents, etc. The provided STR typing system and kit can achieve single-tube amplification of the 58, 119, or 179 linked autosomal STR gene loci, are good in balance, high in sensitivity, good in specificity, and accurate in typing results, and can be used to identify complex consanguinity relationships of different consanguinity relationships at a same level.

The invention claims a linked autosomal short tandem repeat (STR) typing system based on a high-throughput sequencing technology and a kit, and relates to the technical field of nucleic acid in-vitrodetection. The STR typing system includes PCR primers for amplification of 61, 121 or 179 linked autosomal STR loci, and the kit includes a PCR primer combination, an Index linker sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancing agent NB, a YF buffer liquid B and a library building reagent. The STR typing system and kit provided by the invention can realize single-tubeamplification of the 61, 121 or 179 linked autosomal STR loci, have good balance, high sensitivity, good specificity, and accurate typing results, and can be used to identify complex genetic relationships of different genetic relationships at a same level.

The invention discloses a graph Web display method based on a panorama for an industrial monitoring system. The method includes the steps of using a panorama scene editor to import a panorama resource set and edict and generate a panorama monitoring engineering file set; exporting the panorama monitoring engineering file set as a Web item file set to be imported into a Web server for conducting Web display. According to the method, construction is conducted on the basis of the panorama; the graph Web display method has the advantages of being not only short in development period of two-dimensional graphs and low in cost, but great in universality and interactivity of three-dimensional models, and meanwhile, real scenes can be restored to the maximum degree, and the method can cooperate with the existing industrial monitoring systems for usage to meet the demands for display and interactivity in complex scenes.

The invention discloses an establishing method of an osteosarcoma EPI (Epirubicin) drug-resistant cell line. The establishing method comprises the following step: by taking human 143b cells as parentcells and EPI as an induction drug, jointly implementing a drug gradual increment induction method and a large dosage impact induction method with in a drug concentration range of 3 to 120 nM/L untilobtaining a drug-resistant cell line which can be in normal growth and passage under the drug concentration of at least 120 nM/L. The establishing method disclosed by the invention has the characteristics of short establishing period and stable drug resistance of the drug-resistant cell line, and the established drug-resistant cell line can be used for research on an osteosarcoma EPI drug-resistant mechanism and research on reversing drug resistance.

The invention discloses a genetic engineering bacterium producing beta-ionone with high yield and a construction method and application of the genetic engineering bacterium, and belongs to the field of microbial fermentation. In the method, by strengthening MVA pathway and introducing phosphotransketase pathway, the supply of acetyl-CoA in cytoplasm and the metabolic flux of terpenoid synthesis pathway are increased, so that the genetic engineering bacterium producing beta-ionone with high yield is successfully constructed and obtained. The genetic engineering bacteria obtained according to the method of the invention can produce beta-ionone under conventional fermentation conditions, the fermentation yield in a shake flask can reach about 358 mg/L, and the fermentation yield in a 3L fermentor can reach about 0.98 g/L, which is close to an industrialization level. According to the present invention, the perfume beta-ionone can be produced by utilizing a simple medium for fermentation,one-time and highly efficient transformation and integration of multiple genes can be realized, the construction time of the engineering bacteria can be significantly shortened, the perfume beta-ionone can be produced by the obtained engineering bacteria with simple carbon sources such as glucose and glycerin for fermentation, and the engineering bacteria has good industrial application prospects.

The invention discloses a preparation method of a fusion mutant of Ebola virus glycoprotein and matrix protein by saccharomycetes. The preparation method includes steps of 1), introducing gene for encoding the fusion mutant of Ebola virus glycoprotein and matrix protein to a receptor yeast cell to obtain the recombined yeast cell for expressing the gene; 2), orderly cultivating and breaking the recombined yeast cell to obtain the fusion mutant of Ebola virus glycoprotein and matrix protein from the broken product. The fusion mutant of Ebola virus glycoprotein and matrix protein is a recombined protein obtained by fusing an Ebola virus glycoprotein core zone reserved with an Ebola virus glycoprotein receptor bond zone and a glycan cap zone to an N terminal of the Ebola virus matrix protein. The fusion mutant of Ebola virus glycoprotein and matrix protein has a polymer structure and has galactosylated modification; therefore, the fusion mutant is potential to be a vaccine for preventing Ebola hemorrhagic fever.