Human Y chromosome 37 STR loci fluorescence labelled multiplex amplification kit and application thereof

A Y-chromosome and fluorescent labeling technology, applied in the field of forensic genetics, can solve the problems of low individual identification between male samples and weak identification of close relatives of male individuals, and achieve high sensitivity, accurate typing results, and strong specificity. Effect

Active Publication Date: 2018-08-24
AGCU SCIENTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the test kits in the prior art have the defects of weak individual discrimination between close relatives of males and low individual discrimination between male samples

Method used

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  • Human Y chromosome 37 STR loci fluorescence labelled multiplex amplification kit and application thereof
  • Human Y chromosome 37 STR loci fluorescence labelled multiplex amplification kit and application thereof
  • Human Y chromosome 37 STR loci fluorescence labelled multiplex amplification kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Gene locus screening

[0043] 通过对DYS19、DYS385a / b、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS635、Y_GATA_H4、DYS481、DYS533、DYS576、DYS643、DYS460、DYS549、DYF387S1a、 DYF387S1b、DYS449、DYS518、DYS627、DYS570、DYS527a / b、DYS447、DYS444、DYS557、DYS596、DYS446、DYS510、DYS622、DYS443、DYS587、DYS522、Y_GATA_A10、DYS520、DYS552、DYS593、DYS531、DYS459a / b、DYS508、 DYS388, DYS617, DYS645, DYS713, DYS630, DYF404S1, DYS626, DYS526a / b, a total of 59 Y-chromosomal short tandem repeat loci and their allelic genetic characteristics were thoroughly studied, and 37 Y-chromosome short tandem repeats were screened out 序列基因座:DYS392、DYS389I、DYS447、DYS389II、DYS438、DYS527a、DYS527b、DYS645、DYS596、DYS391、DYS456、DYS19、DYS593、DYS448、DYS627、DYS557、DYS437、DYS481、DYS533、DYS390、DYS385a、DYS385b、DYF387S1a、 DYF387S1b, DYS460, DYS393, Y_GATA_H4, DYS439, DYS635, DYS444, DYS643, DYS549, DYS576, DYS458, DYS570, DYS449, DYS518, these 37 Y chromosome short tandem repeat loci have low g...

Embodiment 2

[0059] Application in embodiment 2 family investigation

[0060] (1) Collection of blood spots in the case: The samples were provided by a public security bureau.

[0061] (2) Sample DNA extraction: the silica bead extraction method in the forensic DNA laboratory test specification [GA-T383-2002] was used.

[0062](3) Use the AGCU Database Y30 kit to perform typing tests on the above two samples, and then compare the typing results with this kit. The typing results are compared in the following table:

[0063]

[0064]

[0065] The results showed that the typing of the suspect and the on-site samples were consistent, but there was a difference between the DYS576 and DYS449 rapid mutation loci between the suspect’s father and the on-site samples, so the suspicion of the suspect’s father was ruled out during the case handling process, and the AGCUDatabase Y30 kit because Without DYS576 and DYS449, the father and son cannot be distinguished. It can be seen from this that ...

Embodiment 3

[0066] Application in Example 3 Paternity Test

[0067] (1) Collection of oral exfoliated cells: The samples were provided by a judicial appraisal institution.

[0068] (2) Sample DNA extraction: the silica bead extraction method in the forensic DNA laboratory test specification [GA-T383-2002] was used.

[0069] (3) Data comparison of sample test results: see the amplification spectra of uncle and nephew respectively Figure 4 and 5 , and the classification results are compared in the following table:

[0070]

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PUM

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Abstract

The invention discloses a human Y chromosome 37 STR loci fluorescence labelled multiplex amplification kit and an application thereof. The kit comprises a specific primer for amplifying 37 Y-STR lociwhich comprise 30 low-mutation Y-STR loci and 7 rapid-mutation Y-STR loci. The kit comprises 30 low-mutation and 7 rapid-mutation rate high-polymorphism Y-STR loci, considers distinguishing of checking of a male family and different male individuals of a same male line, and is the Y-STR detection product with a maximum loci quantity on market. The primer in the kit has the advantages of strong specification, high sensitivity, and accurate parting result, and practical case examination, DNA database construction and paternity identification requirements can be completely satisfied. The kit hasstrong check-material adaptability.

Description

technical field [0001] The invention belongs to the field of forensic genetics, and relates to a fluorescent label compound amplification inspection system, in particular to a fluorescent label compound amplification kit for 37 STR loci of human Y chromosome and its application. Background technique [0002] In the 1990s, fluorescently labeled short tandem repeat loci (short tandem repeat, STR) were first proposed and used as genetic markers for forensic evidence related research and identification. Compared with other genetic markers, STR markers have small fragments and are easy to amplify, and are more suitable for trace and degraded samples. Multiple STR loci can be simultaneously amplified, mixed samples, and DNA molecule degradation detection. Difficult biological samples such as biological samples and trace biological samples can be successfully tested and typed by DNA, so they have the advantages of fast, efficient, accurate, sensitive, and large amount of informatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156
Inventor 郑卫国杜蔚安刘超刘宏王邦超刘长晖张雷史明皓梅兴林郭育林
Owner AGCU SCIENTECH
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