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Method and system for obtaining DIP-STR (Deletion/Insertion Polymorphism-Short Tandem Repeat polymorphism) gene locus typing result of DNA (deoxyribonucleic acid) of unknown individual source in mixed spot

A technology of DIP-STR and DNA typing, which is applied in the direction of biochemical equipment and methods, measurement/inspection of microorganisms, etc., and can solve the problems that there is no DIP-STR compound amplification system, etc.

Active Publication Date: 2020-01-31
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the DIP-STR multiplex amplification system at home and abroad

Method used

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  • Method and system for obtaining DIP-STR (Deletion/Insertion Polymorphism-Short Tandem Repeat polymorphism) gene locus typing result of DNA (deoxyribonucleic acid) of unknown individual source in mixed spot
  • Method and system for obtaining DIP-STR (Deletion/Insertion Polymorphism-Short Tandem Repeat polymorphism) gene locus typing result of DNA (deoxyribonucleic acid) of unknown individual source in mixed spot
  • Method and system for obtaining DIP-STR (Deletion/Insertion Polymorphism-Short Tandem Repeat polymorphism) gene locus typing result of DNA (deoxyribonucleic acid) of unknown individual source in mixed spot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1. Verification of the accuracy of a method and system for obtaining DIP-STR locus typing results of DNA of unknown individual origin in mixed spots of the present invention

[0069] Take 0.5ml blood sample respectively to 3 volunteers, be respectively sample 1, sample 2, sample 3, altogether 3 parts; Sample 1, sample 2, sample 3 are mixed respectively, make mixed spot sample 12 (sample 1 and sample 3 Mixed sample 2), mixed stained sample 13 (mixed sample 1 and sample 3), mixed spotted sample 23 (mixed sample 2 and sample 3), stored at -20 °C.

[0070] Assume that samples 1 and 3 are suspected suspect samples, and sample 2 is a victim sample (DNA from a single individual whose source is known). Currently, a mixed sample 12 (mixed DNA) of the suspect and the victim is obtained from the case scene. At present, it is necessary to apply the present invention to deduce and exclude criminal suspects from samples 1 and 3.

[0071] Using the systems and methods of the ...

Embodiment 2

[0103] Example 2 Verifies the accuracy of a method and system of the present invention for inferring the origin of DNA individuals in mixed blotches

[0104] In this embodiment, samples 1, 2, 3 and the mixed stain samples (mixed stain sample 12, mixed stain sample 13, and mixed stain sample 23) are the same as those in embodiment 1.

[0105] Figure 5 Typing results for mixed blob sample 13 are shown, Image 6 Typing results for mixed blob sample 23 are shown.

[0106] Table 7 shows the typing results of samples 1, 2, 3 and mixed spotted sample 12, mixed spotted sample 13, and mixed spotted sample 23. The typing results from Table 7 can be compared with each other to verify the accuracy of the typing of the system of the present invention.

[0107] Table 7

[0108]

[0109]

[0110] Also use a conventional detection system, such as a sequencing system, to recollect blood samples from three volunteers to obtain the typing results of the above-mentioned 13 loci, and co...

Embodiment 3

[0114] Example 3 Using the system and method of the present application to detect the typing of 13 DIP-STRs of human male DNA standard product 9948

[0115] Male DNA standard product 9948 was purchased from Suzhou Xinhai Company with a concentration of 10 ng / μl. The 9948 standard was diluted into different concentration gradients of 0.5ng / μl, 0.25ng / μl, 0.125ng / μl, 0.0625ng / μl, and 0.0313ng / μl.

[0116] Construct a 10 μl amplification system, and add 1 μl of the above-mentioned 9948 standard with different concentration gradients to each 10 μl amplification system, and make 3 replicates for each gradient. Utilize the system and scheme of the present application, carry out classification according to the step of embodiment 1; Figure 7 The typing percentage obtained at each concentration gradient is shown; it can be seen that the standard 9948 can obtain 100% typing at a concentration above 0.0625ng / μl, and can obtain 86% typing at a concentration of 0.0313ng / μl. It shows tha...

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Abstract

The invention provides a method and a system for obtaining a DIP-STR (Deletion / Insertion Polymorphism-Short Tandem Repeat polymorphism) gene locus typing result of the DNA (deoxyribonucleic acid) of an unknown individual source in a mixed spot. The method comprises the following steps of: obtaining the mixed DNA in the mixed spot and the DNA of a single individual of which the individual source isknown; obtaining the mixed typing result of the mixed DNA in the mixed spot, and a typing result, i.e., a DNA typing result of the known individual source, of the DNA of the single individual of which the individual source is known; and according to the typing result, obtaining a typing result of the DNA of the unknown individual source in the mixed spot. The invention also provides a system forthe DIP-STR gene locus typing result of the DNA of the unknown individual source in the mixed spot. By use of the scheme of the invention, a specific DIP-STR gene locus typing result is comprehensively analyzed to effectively obtain the DNA typing result of the unknown individual source, and powerful technical support is provided for public security organizations to quickly determine criminal suspects.

Description

technical field [0001] The present invention relates to a method and system for DIP-STR loci typing of human DNA, in particular to a method and system for obtaining DIP-STR loci typing results of DNA of unknown individual origin in mixed spots. Background technique [0002] For forensic evidence inspection work, the inspection of mixed spots, especially the inspection and identification of trace and extremely unbalanced mixed spots, has always been a difficult problem to be solved. Typically, the donors of the minor component DNA (less than 10%) in mixed plaques are suspects, but are masked by the major component DNA. Because in most cases, the biological traces left by the homicide will contain a large amount of DNA of the victim and a small amount of DNA of the suspect. For example, the victim’s clothes, hair, skin or objects may be touched by the suspect, which contains A small number of suspects' DNA and a large number of victims' DNA were collected. In general, the li...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156
Inventor 莫晓婷崔杨程尚蕾马温华姚伟静丁光树綦雪峰王郗陈宝文李万水
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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