Composite amplification method and kit for 22 short tandem repeats (STR)

A technology of compound amplification and kit, applied in the field of fusion protein
CN106906292AInactive Publication Date: 2017-06-30ACADEMY OF FORENSIC SCIENCE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ACADEMY OF FORENSIC SCIENCE
Publication Date
2017-06-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a composite amplification method and kit for STR loci. The composite amplification method and kit are used for amplifying 22 STR loci and one sex locus, wherein the 22 STR loci are composed of vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, D1S1656, CSF1PO, Penta D, Penta E, TH01, TPOX, D10S1248 and DYS391, and the sex locus is Amelogenin. Amplimers used in the composite amplification method and kit comprise sequences as shown in SEQ ID No. 1 to 46. The composite amplification method and kit provided by the invention can acquire information about the 22 STR loci with good polymorphism and high individual discrimination power and about the one sex locus through simultaneous amplification at a time; and a composite amplification system composed of the loci has good balance, high sensitivity and high specificity, is accurate in typing results and can totally meet demands of judicial expertise.
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Description

technical field

[0001] The invention relates to the technical field of fusion proteins, relates to the detection of polymorphic genetic marker genes in the human genome, and in particular to a method for compound amplification of 22 STR (short tandem repeat) loci and 1 sex locus and its Reagent test kit. Background technique

[0002] Short tandem repeats (STR for short), also known as microsatellites or simple sequence repeats (SSR), are a type of DNA tandem repeats widely present in eukaryotic genomes, with a core sequence of 2-6 base repeat units. The number of STR loci is large and widely distributed, accounting for about 3% of the entire genome (International Human Genome Sequencing Consortium, 2001), and the polymorphism is high. Species differences follow the Mendelian law of inheritance in the genetic process. Therefore, STR amplification detection technology is widely used in forensic individual identification, kinship identification and population genetics researc...

Claims

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