Detection method of th gene snp locus associated with opioid dependence and its application

A substance-dependent and opioid-based technology, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve problems such as unreported relationship of dependence, achieve accurate typing results, broad application prospects, and rapid genetic analysis. type effect

Active Publication Date: 2021-09-07
XI AN JIAOTONG UNIV
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Problems solved by technology

[0005] So far, with the continuous development of high-throughput and high-precision DNA research technology, the research on the molecular genetic mechanism related to drug addiction has also developed rapidly, and new candidate genes have been reported continuously; the current research on rs10770140 in TH gene Studies on polymorphisms have only been found in relevant reports on the mechanism of hypertension, but the relationship between rs10770140 and opioid dependence has not been reported yet

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  • Detection method of th gene snp locus associated with opioid dependence and its application
  • Detection method of th gene snp locus associated with opioid dependence and its application
  • Detection method of th gene snp locus associated with opioid dependence and its application

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Embodiment Construction

[0028] The present invention is further described below with reference to the accompanying drawings and examples, and the examples of the invention are not intended to limit the scope of the invention.

[0029] The present invention analyzes the sequence of the SNP site RS 10770140 to determine the genotype of the TH gene RS 10770140 polymorphic site by extracting the human genomic DNA, and found that the multi-state site is closely related to the OD sensibility of the subject: When the genotype of the TH gene RS10770140 is a C / C homozygous, the subject is the highest inductive; the genotype of the TH gene RS 10770140 polymorphic site is a C / T heterozygate, the susceptibility of the subject Higher; the genotype of the TH gene RS 10770140 polymorphic site is a T / T homogenizer, the subject's susceptibility is the lowest. Based on the above discovery, the present invention proposes an OD high-risk population screening method and screening kit for opioid substance dependent (OD)...

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Abstract

The invention discloses a method for detecting TH gene SNP sites related to opioid dependence and its application. According to the correlation between TH gene SNP site rs10770140 polymorphism and opioid dependence (OD), the invention designs Provided are specific primers for amplifying the sequence fragments where TH gene polymorphism sites are located, and provide a SNP typing detection kit and typing detection method containing the primers. The invention can perform genotyping on the rs10770140 locus, the method is simple and fast, the result is accurate and clear, and provides a new way for OD susceptibility identification.

Description

Technical field [0001] The present invention belongs to the field of gene detection and analysis, involves tyrosine hydroxylase, TH) gene mononucleotide polymorphism (SINGLENUCLETIDE POLYMORPHISM, SNP), and SINGLETIDE POLYMORPHISM, SNP Detection detection and application of site RS10770140. Background technique [0002] Drug-dependence is a chronic recurrent brain disease that is characterized by forced topherion and laughter of drugs, which causes serious harm to individuals and society. The opioid substance is an alkaloid and derivative extracted from poppy plants. Among them, morphine is currently the most effective analgesic medicine, and the number of morphine analgesia has increased in the clinical and advanced cancer patients. However, due to its significant dependence and tolerance, clinical applications have been limited, and opioid prescription drugs have become the primary goal of non-medical drug abuse. In the last three years, the United States is suffering from incr...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858C12Q1/6883
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2531/113
Inventor 阎春霞李少卿张宝朱峰杨婧思刘军林张玉向吴亚楠
Owner XI AN JIAOTONG UNIV
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