Method for detecting TH gene SNP locus related to opioid dependence and application thereof

A technology of material dependence and detection method, which is applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of unreported relationship of dependence, achieve accurate typing results, fast genotyping, and application prospects broad effect

Active Publication Date: 2019-10-15
XI AN JIAOTONG UNIV
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Problems solved by technology

[0005] So far, with the continuous development of high-throughput and high-precision DNA research technology, the research on the molecular genetic mechanism related to drug addiction has also developed rapidly, and new candidate genes have been reported continuously; the current research on rs10770140 in TH gene Studies on polymorphisms have only been found in relevant reports on the mechanism of hypertension, but the relationship between rs10770140 and opioid dependence has not been reported yet

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  • Method for detecting TH gene SNP locus related to opioid dependence and application thereof
  • Method for detecting TH gene SNP locus related to opioid dependence and application thereof
  • Method for detecting TH gene SNP locus related to opioid dependence and application thereof

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Embodiment Construction

[0028] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, which are to explain the present invention rather than limit the protection scope of the present invention.

[0029] In the present invention, the sequence of the SNP site rs10770140 is studied, and the genotype of the subject's TH gene rs10770140 polymorphic site is determined by extracting human genomic DNA, and it is found that the polymorphic site is closely related to the subject's susceptibility to OD: When the genotype of TH gene rs10770140 polymorphic site is C / C homozygous, the subject has the highest susceptibility; when the genotype of TH gene rs10770140 polymorphic site is C / T heterozygous, the subject's susceptibility Higher; when the genotype of TH gene rs10770140 polymorphic site is T / T homozygous, the susceptibility of the subject is the lowest. Based on the above findings, the present invention proposes a screening method and screening ki...

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Abstract

The invention discloses a method for detecting a TH gene SNP locus related to opioid dependence and application thereof. According to correlation between polymorphism of TH gene SNP locus rs10770140 and opioid dependence (OD), specific primers used for amplification of a sequence fragment where the TH gene polymorphic locus is are designed. An SNP typing detection kit including the primers and a typing detection method are also provided. The locus rs10770140 can be subjected to gene typing. The method is simple, convenient and rapid, provides a clear and accurate result, and provides a novel route for OD susceptibility identification.

Description

technical field [0001] The invention belongs to the field of gene detection and analysis, and relates to a tyrosine hydroxylase (tyrosine hydroxylase, TH) gene single nucleotide polymorphism (singlenucleotide polymorphism, SNP) related to opioids dependence (opioids dependence, OD) susceptibility ) type detection and application of rs10770140 locus. Background technique [0002] Drug dependence is a chronic recurrent brain disease characterized by compulsive drug-seeking and persistent craving for drugs, which causes serious harm to individuals and society. Opioids are alkaloids and derivatives extracted from poppy plants. Among them, morphine is currently the most effective analgesic in clinical practice. The number of patients with advanced cancer who use morphine for analgesia increases by about 13 million per year. However, due to its significant dependence and tolerance, clinical application is subject to many restrictions, and opioid prescription drugs have also becom...

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Application Information

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IPC IPC(8): C12Q1/6858C12Q1/6883
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2531/113
Inventor 阎春霞李少卿张宝朱峰杨婧思刘军林张玉向吴亚楠
Owner XI AN JIAOTONG UNIV
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