Llinked autosomal (STR typing system based on high-throughput sequencing technology and kit

A technology of autosomes and kits, applied in recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as inability to distinguish complex kinship
CN110734982AActive Publication Date: 2020-01-31SHANXI MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANXI MEDICAL UNIV
Publication Date
2020-01-31

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Abstract

The invention claims a linked autosomal short tandem repeat (STR) typing system based on a high-throughput sequencing technology and a kit, and relates to the technical field of nucleic acid in-vitrodetection. The STR typing system includes PCR primers for amplification of 61, 121 or 179 linked autosomal STR loci, and the kit includes a PCR primer combination, an Index linker sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancing agent NB, a YF buffer liquid B and a library building reagent. The STR typing system and kit provided by the invention can realize single-tubeamplification of the 61, 121 or 179 linked autosomal STR loci, have good balance, high sensitivity, good specificity, and accurate typing results, and can be used to identify complex genetic relationships of different genetic relationships at a same level.
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Description

technical field

[0001] The invention relates to the technical field of nucleic acid in vitro detection, in particular to a linked autosomal STR typing system based on high-throughput sequencing technology and a kit thereof. Background technique

[0002] Short tandem repeat (STR) is a special sequence composed of 2-6bp repeating units in series. STR loci are large in number and widely distributed, accounting for about 3% of the entire genome, and are highly polymorphic. The main reason is that the number of core sequence repeats varies among individuals, and this difference follows the Mendelian law of inheritance in the genetic process. Therefore, STR amplification detection technology is widely used in forensic individual identification, kinship identification and population genetics research. The most commonly used STR typing techniques are fluorescent labeling multiplex amplification combined with capillary electrophoresis (CE) typing techniques and next-generation seque...

Claims

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