Primers, kit and method for detecting short tandem repetitive sequence
A short tandem repeat and kit technology, applied in the field of genetic engineering, can solve problems such as genetic polymorphism differences of short tandem repeat sequence loci, and achieve the effects of low cost, high accuracy and simple operation.
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Embodiment 1
[0050] Example 1: Detection of 18 STR loci in human genomic DNA samples 1
[0051] In this example, magnetic beads were used to extract DNA to obtain human genomic DNA samples; PCR amplification was performed on the obtained DNA samples, and the fluorescent labels of the primers used were 6-FAM, HEX, TAMRA and ROX respectively; the obtained PCR amplification products Capillary electrophoresis detection; typing analysis. At the same time, combined with Life's STR detection kit and Sanger sequencing method to determine the types of 18 STR loci in the obtained human genomic DNA samples. details as follows.
[0052] 1. Configuration of primer composition storage solution
[0053] Prepare four sets of 5× primer storage solutions according to the formula shown in Table 3.
[0054] Table 3, the formula list of four groups of 5× primer storage solutions
[0055]
[0056]
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[0058] 2. PCR amplification
[0059] During PCR amplification, a DNA sample is amplified i...
Embodiment 2
[0070] Example 2: Detection of 18 STR loci in human genomic DNA samples 2
[0071] In this example, the magnetic bead method was used to extract DNA to obtain human genomic DNA samples; PCR amplification was performed on the obtained DNA samples, and the fluorescent labels of the primers used were 6-FAM, HEX, TAMRA and ROX respectively; the obtained PCR amplification products Capillary electrophoresis detection; typing analysis. At the same time, combined with Life's STR detection kit and Sanger sequencing method to determine the types of 18 STR loci in the obtained human genomic DNA samples. details as follows.
[0072] 1. Configuration of primer composition storage solution
[0073] According to the formula shown in Table 5, prepare 5× primer storage solution.
[0074] Formulation table of table 5, 5 × primer storage solution
[0075]
[0076]
[0077] 2. PCR amplification
[0078] During PCR amplification, 18 STR loci of a DNA sample are amplified in an amplifica...
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