Method for judging specific individual attribution of malignant tumor tissues, and application of method

A technology for malignant tumors and individuals, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of STRs mutations, SNPs detection methods are complicated to operate, and the sequencing throughput is low, achieving low mutation rate and detection The method is easy to operate and has strong scalability

Active Publication Date: 2018-09-07
HEBEI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of significant mutations in STRs of malignant tumor tissues, complex operation of SNPs detection methods, and low sequencing throughput, the present invention provides a method for judging the specific individual attribution of malignant tumor tissues

Method used

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  • Method for judging specific individual attribution of malignant tumor tissues, and application of method
  • Method for judging specific individual attribution of malignant tumor tissues, and application of method
  • Method for judging specific individual attribution of malignant tumor tissues, and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This example provides a method for judging the specific individual attribution of malignant tumor tissue: a cancer patient suspects that the hospital misadjusted the tumor tissue wax block after surgery to remove the tumor, resulting in a misdiagnosis, and requests to identify whether the archived tumor tissue wax block comes from himself Include the following steps:

[0035] 1. Reference sample

[0036] Take 0.5ml of the patient's peripheral blood to make a blood card as a reference sample, and dry it for later use.

[0037] 2. Process tumor tissue samples to determine the percentage of stromal cells

[0038] Make 5 μm thick sections with tumor tissue wax blocks, stain with hematoxylin and ethanol (HE), and observe the sections under a microscope. First use 40× magnification to find the most infiltrated area of ​​tumor tissue, and then use 100× magnification to evaluate the tumor The percentage of the area occupied by the cells in the visual field, and the rest is the ...

Embodiment 2

[0054] This example provides a method for judging the specific individual attribution of malignant tumor tissue: a cancer patient suspects that the hospital misadjusted the tumor tissue wax block after surgery to remove the tumor, resulting in a misdiagnosis, and requests to identify whether the archived tumor tissue wax block comes from himself Include the following steps:

[0055] Steps 1 to 6 are the same as in Example 1, wherein the ratio of tumor tissue-to-stromal cells is 30%.

[0056] 7. Check the SNP typing of tumor tissue and peripheral blood samples of patients. (1) "NN" appears in the typing result, indicating that the plug-in cannot judge the type after comprehensive detection of the sequencing data. Download the BAM and BAI files generated after the sequencing data analysis, and import them into the IGV software for visual analysis. According to the principle of min_snp_coverage≥50 and min_allele_frequency>10%, the site is manually typed; (2) All heterozygous loci...

Embodiment 3

[0059] This example provides a method for judging the specific individual attribution of malignant tumor tissue: a cancer patient suspects that the hospital misadjusted the tumor tissue wax block after surgery to remove the tumor, resulting in a misdiagnosis, and requests to identify whether the archived tumor tissue wax block comes from himself Include the following steps:

[0060] Steps 1 to 6 are the same as in Example 1, wherein the ratio of tumor tissue to mesenchymal cells is 10% to 20%.

[0061] 7. Check the SNP classification of tumor tissue, (1) "NN" appears in the typing result, indicating that the plug-in cannot judge the type after comprehensive detection of the sequencing data. Download the BAM and BAI files generated after the sequencing data analysis, and import them into the IGV software for visual analysis. According to the principle of min_snp_coverage ≥ 50 and min_allele_frequency > 10%, the site is manually typed; (2) in tumor tissue classification In the ...

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Abstract

The invention relates to a method for judging specific individual attribution of malignant tumor tissues. The method comprises the following operating steps: respectively extracting cell DNA of the malignant tumor tissues to be tested and cell DNA of the normal tissues of a specific individual; quantifying and diluting the DNA, and then performing genome sequencing by using a next-generation sequencing technology to obtain original genomic data; performing judgment of single nucleotide polymorphism according to the original genomic data; judging the attribution of the malignant tumor tissues and the specific individual based on the result of the judgment. The method has the characteristics of being high in accuracy, strong in expansibility, rapid and flexible, and the like, and can more accurately detect the genotyping of the malignant tumor tissues, thereby being applied to forensic identification of the malignant tumor tissues; the method can be used for judging whether the malignanttumor tissues to be tested are derived from a specific individual or not.

Description

technical field [0001] The invention relates to the technical field of tissue identification, in particular to a method for judging the specific individual attribution of malignant tumor tissue and its application. Background technique [0002] Forensic identification of malignant tumor tissue refers to forensic identification cases in which malignant tumor tissue is used for individual identification and paternity identification. In some cases, malignant tumor tissue may become the only reference or test sample that can provide evidence. For example: family members suspect that the pathology department of the hospital confuses the wax block of malignant tumor tissue, leading to misdiagnosis; cancer patients die and their bodies are cremated, and family members need to conduct paternity testing; insurance companies suspect the source of malignant tumor tissue provided by patients; tsunamis, earthquakes and other mass catastrophic events Conduct paternity testing; law enforce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/156
Inventor 丛斌付丽红李淑瑾马春玲孙丽娟
Owner HEBEI MEDICAL UNIVERSITY
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