SNP markers related to grass carp growth speed and application thereof

A technology of growth rate and grass carp, applied in the field of animal molecular biology DNA marker technology and application, can solve the problems of grass carp fry quality not guaranteed, slow growth, low survival rate, etc.

Active Publication Date: 2017-02-08
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

However, this method of reproduction is often also based on the needs of production. During the breeding process, the broodstock in the production pond ranges from 1 group to 30 groups (1 group is 1 female fish and 1 male fish). The mating and breeding between them is r

Method used

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  • SNP markers related to grass carp growth speed and application thereof
  • SNP markers related to grass carp growth speed and application thereof
  • SNP markers related to grass carp growth speed and application thereof

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Embodiment 1

[0046] 1. Screening of SNP loci

[0047] Four samples of grass carp populations from different sources were selected, namely: Xijiang population (XJ) samples were taken from the Xijiang section of the Pearl River system in 2005, Ningxiang population (NX) samples were taken from the Xiangjiang section of a tributary of the Yangtze River in 2004, Yuanjiang The population (YJ) was collected from the Yuanjiang section, a tributary of the Yangtze River, in 2004, and the Honghu population (HH) sample was collected from Honghu, the mainstream lake of the Yangtze River, in 2004. The samples from the Yangtze River system and the Pearl River system were all wild small grass carp caught from rivers or lakes. After breeding to (13.0±2.0) kg, the fin ray tissue was fixed with absolute ethanol and stored in the Pearl River Fisheries Research Institute at -20°C. A total of 20 individuals, 5 individuals from each group, were used for SNP screening of NPY gene. By comparing the NPY gene seque...

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Abstract

Through amplification of a part of fragments of an NPY gene, and through an SNaPshot SNP typing method, provided is SNP markers related to the grass carp growth speed, wherein S1 is located at a 639th locus from an initiation codon (ATG, with an ATG first nucleotide as the first) in an intron 1 sequence of the NPY gene, and a base at the position is T or A; S2 is located at an 892nd locus from the initiation codon in the intron 1 sequence of the NPY gene, and a base at the position is C or A. The two SNP loci can be used for identification or breeding of grass carp with faster growth speed, and lay the foundation for screening and establishment of grass carp fast-growth new varieties.

Description

[0001] 【Technical field】 [0002] The invention belongs to the field of animal molecular biology DNA marker technology and application, and specifically relates to a SNP marker related to the growth rate of grass carp, a sequence for detecting SNP haplotypes, a pair of primers and the SNP marker for assisting the selection of fast-growing strains of grass carp application in education. [0003] 【Background technique】 [0004] Grass carp (Ctenopharyngodon idella) belongs to the subfamily Leuciscinae (Leuciscinae) and the genus Ctenopharyngodon. It is the breeding object with the largest annual output of freshwater aquaculture fish in my country. Because of its fast growth and delicious meat, grass carp is very popular among people. At present, grass carp farming still has some room for profit, but in terms of production expenditure, feed costs have been rising since 2008, and feed costs account for more than 70% of the cost of grass carp farming. In recent years, the genetic ba...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 樊佳佳朱冰白俊杰姜鹏马冬梅李胜杰
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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