SNP markers related to grass carp growth speed and application thereof

Abstract

Through amplification of a part of fragments of an NPY gene, and through an SNaPshot SNP typing method, provided is SNP markers related to the grass carp growth speed, wherein S1 is located at a 639th locus from an initiation codon (ATG, with an ATG first nucleotide as the first) in an intron 1 sequence of the NPY gene, and a base at the position is T or A; S2 is located at an 892nd locus from the initiation codon in the intron 1 sequence of the NPY gene, and a base at the position is C or A. The two SNP loci can be used for identification or breeding of grass carp with faster growth speed, and lay the foundation for screening and establishment of grass carp fast-growth new varieties.

Description

[0001] 【Technical field】 [0002] The invention belongs to the field of animal molecular biology DNA marker technology and application, and specifically relates to a SNP marker related to the growth rate of grass carp, a sequence for detecting SNP haplotypes, a pair of primers and the SNP marker for assisting the selection of fast-growing strains of grass carp application in education. [0003] 【Background technique】 [0004] Grass carp (Ctenopharyngodon idella) belongs to the subfamily Leuciscinae (Leuciscinae) and the genus Ctenopharyngodon. It is the breeding object with the largest annual output of freshwater aquaculture fish in my country. Because of its fast growth and delicious meat, grass carp is very popular among people. At present, grass carp farming still has some room for profit, but in terms of production expenditure, feed costs have been rising since 2008, and feed costs account for more than 70% of the cost of grass carp farming. In recent years, the genetic ba...

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Problems solved by technology

However, this method of reproduction is often also based on the needs of production. During the breeding process, the broodstock in the production pond ranges from 1 group to 30 groups (1 group is 1 female fish and 1 male fish). The mating and breeding between them is random. Because the genetic information of the parents is unclear, it often leads to inbreeding and the breeding of parents with poor quality. The quality of the grass carp fry produced in this way is not guaranteed, which often leads to low survival rate and slow growth.

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