New 10 Y-chromosome short tandem repeat locus parting method therefor

Abstract

The invention discloses a new kind of ten STR gene loci for Y chromosome and the typing method, which is characterized in that polyacrylamide gel is utilized for electrophoresis typing; the ten STR gene loci for Y chromosome, which can be applied in forensic medicine field, are screened out with silver staining coloration method; furthermore, the allelic ladder of each locus is prepared; a PCR primer and the expansion condition of the Y chromosome STR locus are optimized; wherein the optimization to the PCR primer and the expansion condition and the preparation of allelic ladder can be standardized and simplified, which is suitable for popularization of base unit. The ten STR gene loci for Y chromosome can be applied for individual identification, paternity testing and gene diagnosis in forensic medicine, anthropology, genetics, disease and other field. The new kind of ten STR gene loci for Y chromosome and the typing method has the advantages of wide application prospect, which is particularly suitable for paternity testing in the condition of patrilateral loss such as death or missing in forensic medicine practice and individual identification of mixed stain in rape and gang-rape cases, in particular to the identification to suspect having azoospermia or oligospermia disease.

Description

technical field [0001] The invention relates to a nucleic acid detection method, which relates to the detection of polymorphic genetic markers in the human genome, in particular to a new 10 Y chromosome STR gene loci and a typing method thereof. It is suitable for the research of individual identification, paternity identification and genetic diagnosis in the fields of forensic science, anthropology, genetics and diseases, etc. Applied research provides basic data. Background technique [0002] Short tandem repeats (Short Tandem Repeat, STR) are composed of 2-6 nucleotide repeats to form a specific sequence. STR is a kind of genetic marker widely distributed in human genome and highly polymorphic. Short tandem repeat sequences have the following significant advantages in research and application: small fragment length, easy to be amplified by PCR and electrophoresis typing; material detection; PCR amplification results are stable, and there are few additional bands and sha...

AI Technical Summary

Problems solved by technology

[0019] (1) Commercial kits only include the established Y-STR loci, and it is difficult to add genetic markers when using such kits;

[0020] (2) These Y-STR loci were developed based on data from groups other than the Chinese population (mainly the Caucasian population). lower

[0021] (3) Foreign commercial kits are expensive

[0022] The above shortcomings limit the application of Y chromosome STR genetic markers in China, which is not conducive to further promotion at the grassroots level