Method for evaluating hypoimmunity of methamphetamine addict

Abstract

The invention relates to a method for evaluating hypoimmunity of a methamphetamine addict, which comprises the following steps: S1, collecting peripheral venous blood of a subject, and centrifugally separating the peripheral venous blood to obtain plasma; s2, carrying out enzyme-linked immunosorbent assay (ELISA) detection on IL-1beta, S100A8, IFN gamma and ISG15 on the plasma to obtain corresponding plasma protein levels of the IL-1beta, the S100A8, the IFN gamma and the ISG15; and S3, comparing the detected plasma protein levels of the IL-1beta, the S100A8, the IFN gamma and the ISG15 with the plasma protein levels of the IL-1beta, the S100A8, the IFN gamma and the ISG15 of a normal person, and when the detected plasma protein levels are reduced by more than 50% compared with the plasma protein levels of the normal person, considering that the immunity of the subject is reduced and the infection risk is increased. A detection sample is a conventional blood sample, the dosage is small, and sampling is convenient; the detection reagent, the instrument and the detection process are simple and convenient.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for evaluating the hypoimmunity of methamphetamine addicts. Background technique [0002] Meth is a non-catecholamine sympathomimetic central stimulant, which can selectively act on the central nervous system above the brain stem, increase the excitability of the cerebral cortex, and enhance the activity of the central nervous system. Studies have shown that the excitatory effect of methamphetamine is mainly related to the increase in the content of monoamine neurotransmitters (including dopamine, norepinephrine, 5-hydroxytryptamine, etc.) in the synaptic cleft. Meth promotes the release of dopamine from nerve endings; inhibits the dopamine transporter from re-uptake of dopamine from the synaptic cleft; inhibits the storage of dopamine synthesized in the cytoplasm to vesicles through vesicular monoamine transporter 2; inhibits the metabolism of monoamine oxidase to in...

AI Technical Summary

Problems solved by technology

[0004] However, in the existing technology, there is no simple and effective method to evaluate the impact of methamphetamine on the body's immunity, as well as the immunity of methamphetamine addicts

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