36 results about "ISG15" patented technology

The instant invention provides methods for the treatment and prevention of viral infection, e.g., HIV infection, based on the discovery that viral replication utilizes the ubiquitination pathway of the host cell to replicate. Specifically, the invention provides methods for the treatment and prevention of viral infection, e.g., HIV infection, by modulation of ISG15 conjugation and deconjugation, e.g., by modulation of the activity or expression of UBP43 or UBEL-1.

The disclosure relates to a system for identifying TCM syndrome types of steroid-induced necrosis of the femoral head through molecular markers, wherein the molecular markers include CD28, CD4, PLCG1, PRKCA, PTGS2, SOS2, STAT6, TLR4, IFIT1, IRF7, ISG15, MAPK14 and RHOU. The disclosure can be applied to distinguish different TCM syndrome types of steroid-induced femoral head necrosis by detecting the expression of molecular markers, and assist in early diagnosis, providing an effective basis for guiding clinical diagnosis and treatment.

The invention belongs to the field of biotechnology, and in particular relates to the construction of E184L gene deletion attenuated African swine fever virus strain and its application as a vaccine. The present invention first discovered that the African swine fever virus E184L protein can inhibit poly(dA:dT)-induced activation of IFN-β, NF-κB and ISRE promoters, inhibit poly(dA:dT)-induced IFN-β and its downstream The production of cytokines such as factors ISG15, ISG54, IL-6, MCP1 and TNF-α has a strong immunosuppressive effect and can be used as an immunosuppressant application; secondly, the present invention has the effect of genetic engineering on the African swine fever parent strain ASFV In CN / GS / 2018, the immunosuppressive function of the protein encoded by the E184L gene was deleted, which reduced the immunosuppressive activity of the parental strain, and obtained a highly safe attenuated African swine fever candidate vaccine strain; the attenuated African swine fever After the candidate vaccine strain immunizes pigs, it reduces the immunosuppressive activity and pathogenicity of pigs, and can induce significant neutralizing antibody production after immunization. It can be used as a safe and effective candidate vaccine strain for the prevention and control of African swine fever. great social value.

The invention belongs to the technical field of the livestock genetic engineering, and particularly relates to the molecular mark used as auxiliary choice of pig marks and relating to immunity and reproductive trait and application thereof. The molecular mark is obtained from ISG15 gene clone, and the cDNA sequence thereof is described in a sequence list SEQ ID NO: 1. A base mutation of A390-G390 exists in a 390bp place of the sequence list SEQ ID NO: 1, thereby resulting in the HindIII-RFLP polymorphism. The invention also discloses the primers used by the HindIII-RFLP polymorphism and a polymorphism detection method, and provides a new molecular mark for the auxiliary choice of pig marks.

Provided are methods, materials and kits for analyzing DNA samples from bovine to determine whether the animal is a recessive carrier of a genetic mutation that is associated with arthrogryposis multiplex (AM). DNA-containing samples are analyzed by genetic testing to determine whether or not a deletion mutation is present in one of the alleles that are responsible for the AM genetic mutation. In an aspect the deletion encompasses the entirety of the ISG15 ubiquitin-like modifier (ISG15) gene. In an aspect the deletion further encompasses one or both of the 5′ regulatory region of the hairy and enhancer split 4 (HES4) and of the agrin (AGRN) gene and of the first two exons of the AGRN gene.

The invention relates to a detection system for detecting a human immune state and belongs to the technical field of gene detection. The detection system provided by the invention is used for detecting 20 genes, including IFI27, ISG15, IFI44L, RSAD2, HERC5, IFITM3, IFI44, IFIT3, EPSTI1, IFIT1, HP, ANXA3, ARG1, FCGR1B, S100A12, MMP9, IL18R1, TLR3, GYG1 AND FCGR1A in peripheral blood; the assessment for the human immune state can be realized in the manner of increasing or reducing the expression level of each gene; and the genes are utilized to guide accurate medical treatment, including disease accurate parting, prognosis, treatment tracking and therapeutic evaluation. The detection system has excellent clinic application values and wide application prospects.