The invention provides a method for screening Ifn[beta], Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4 agonists and antagonists. The invention relates to the field of biological medicine, and in particular relates to a method for screening antiviral gene agonists and antagonists by measuring the expression quantities of Ifn[beta], Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4and by constructing a vesicular stomatitis virus susceptible cell model caused by overexpression of RXRs (retinoid X receptors). The invention provides a constructing method of the vesicular stomatitis virus susceptible cell model caused by the overexpression of the RXRs, and applications of the RXRs in screening the Ifn[beta], Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4 agonists, and finds that a COX sensing pathway inhibits antiviral genes in a host immune defense system through an RXR-dependent manner, and therefore, the practical application value of the RXRs in screening the Ifn[beta], Isg15, Gbp-1, Oas2, Irf7, Isg20 and Ifn4 agonists is further confirmed.