Application of African swine fever virus D205R and D345L genes

An African swine fever virus, D205R technology, applied in the field of biomedicine, can solve problems such as missing vaccine development
CN113304256AActive Publication Date: 2021-08-27FUJIAN AGRI & FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUJIAN AGRI & FORESTRY UNIV
Publication Date
2021-08-27

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Abstract

The invention discloses application of African swine fever virus D205R and D345L genes, and particularly relates to application of the African swine fever virus D205R and D345L genes in preparation of an African swine fever virus vaccine. The African swine fever virus D205R and D345L genes can significantly inhibit virus infection induced interferon expression, and inhibit interferon stimulation gene ISGs expression. Through dual-luciferase reporter gene system detection, the invention finds that through comparison, sendai-virus-induced interferon promoter activity and interferon stimulation response element (ISRE) activity can be inhibited through expression of the D205R and D345L genes. The fluorescent quantitative PCR test finds that the expression of the D205R and D345L genes can significantly inhibit the mRNA expression level of I-type interferon IFNbeta induced by Sendai virus infection, and inhibit the expression level of interferon stimulating genes ISG15 and OASL playing an antiviral function; and in conclusion, two kinds of African swine fever virus proteins with the function of inhibiting host cell antiviral innate immune response are identified, and a new choice is provided for preparing an African swine fever virus gene deletion vaccine.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and relates to the functional identification of two viral proteins and their application in the preparation of gene deletion vaccines, and mainly relates to the functional identification of African swine fever virus D205R and D345L proteins and their application in the preparation of African swine fever virus gene deletion vaccines in the application. Background technique

[0002] African swine fever virus (ASFV) is a DNA virus and the only member of the African swine fever virus family, the genus ASFV. African swine fever (African swine fever, ASF) is a severe infectious disease of pigs caused by ASFV, which can cause 100% death of infected domestic pigs. ASF first broke out in Kenya in the 1920s, then spread rapidly in South and East Africa, and was introduced to the Indian Ocean region in 1998. After the first outbreak outside Africa (Portugal) in 1957, ASF outbreaks were subsequently reported in ...

Claims

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