39 results about "Pcr test" patented technology

The present application provides a PCR test tube with a small tube cavity volume, which can reduce the reaction system, and at the same time can contact heat transfer over a large area and change temperature quickly. The whole tube is flat, including a tube body with an opening at the upper end and a tube cover for sealing the opening, and a tube cavity communicating with the opening is provided in the lower part of the tube body. There are two channels in the tube body; the lower ends of the two channels are in communication with the tube cavity, but the height of the part communicating with the tube cavity is different; the channel at a higher position in communication with the tube cavity is the air inlet channel, and the channel at a lower position in communication with the tube cavity is the reflux channel; the outlet at the upper end of the air inlet channel and the inlet at the upper end of the reflux channel are both located on the surface of the tube body in contact with the tube cover.

The invention relates to the technical field of antibacterial preparations, and discloses an application of etoposide in an antibacterial preparation and the antibacterial preparation, the antibacterial preparation is a biofilm inhibitor, and etoposide inhibits formation of a biofilm or destroys the formed biofilm by down-regulating gene expression of the bacterial biofilm, so that the antibacterial effect of etoposide is improved. The biofilm is a biofilm of streptococcus mutans ATCC700610, and the biofilm is a biofilm of streptococcus mutans The antibacterial preparation comprises an antibacterial drug, an oral care product, a medical coating and an anti-caries preparation. MIC and MBC determination tests, growth curve tests, crystal violet staining tests, bacterium living / death staining tests and qRT-PCR tests prove that etoposide can effectively down-regulate biofilm related gene expression (such as gtfB, gtfC, gtfD and the like), inhibit formation of a bacterial biofilm or destroy a formed biofilm, and achieve an antibacterial effect.

The invention relates to application of golden larch bark acetic acid in preparation of methicillin-resistant staphylococcus aureus (MRSA) biological membrane and bacterium holding inhibitor. A biological membrane formation inhibition experiment shows that the pseudolaric acid can remarkably inhibit formation of an MRSA biological membrane, a fluorescent quantitative PCR experiment shows that the pseudolaric acid remarkably reduces transcription of related genes of the MRSA biological membrane, and a persistent bacterium killing experiment shows that the pseudolaric acid enhances the killing capability of gentamicin on MRSA persistent bacteria. Lung epithelial cell adhesion tests find that the golden larch bark acetic acid significantly reduces the adhesion effect of the MRSA strain on epithelial cells. A test for constructing an MRSA infected greater wax moth model shows that the golden larch bark acetic acid has a system protection effect on the MRSA infected greater wax moth model.

The invention provides medical application of sanguinarine in preparation of a salmonella flagellum inhibitor, and belongs to the technical field of biological medicine. According to the invention, the flagellum of salmonella is used as a potential target for researching and developing the anti-salmonella toxicity drug, and a natural product capable of inhibiting the flagellum of salmonella is screened through a salmonella sliding motion inhibition test and a real-time fluorescent quantitative PCR test; in combination with tests such as a minimum inhibitory concentration determination test, a growth curve, inhibition of salmonella from adhesion to Caco-2 cells and the like, it is verified that sanguinarine can effectively inhibit the salmonella flagellum-mediated swimming ability on the basis of not influencing normal growth of bacteria, so that the pathogenic potential of salmonella is remarkably reduced, the effect of bacteriostasis instead of sterilization is achieved, and the application prospect is broad. A new research thought and a candidate natural compound are provided for prevention and treatment of salmonella infection, and potential practical application value is achieved.

A method for predicting case counts for future dates in one or more geographic areas of a person infected with a disease is disclosed. The presence of the disease in a biological sample can be tested by a polymerase chain reaction (PCR) test. The load of the one or more pathogens associated with the disease is associated with a PCR cycle that indicates the presence of the one or more pathogens, referred to as a threshold cycle (Ct). Data related to predicting a case count including Ct data and other data is received. The Ct data includes Ct values from a PCR test of a biological sample of a person within the one or more geographic regions. An array of feature data processed by the trained machine learning model is generated, the feature data including Ct features obtained from the data and other features. A predicted number of infected persons is generated by processing the array using machine learning.

The utility model relates to the field of testing apparatus, concretely relates to a PCR test equipment, including PCR appearance body, still including the test tube frame that can be accomodated in PCR appearance body sample tank top, be equipped with a plurality of placing groove in test tube frame, be equipped with clamping piece in a plurality of placing groove, the clamping piece includes first clamping plate and second clamping plate, first clamping plate and second clamping plate opposite side all are equipped with a plurality of arc grooves, first clamping plate and second clamping plate combination, arc groove can be used for placing PCR tube, first clamping plate and second clamping plate all can slide out upward, when using, take out test tube frame, cooperate with a plurality of arc grooves of first clamping plate and second clamping plate, place PCR tube, then adjust injection to be detected sample, adjust end after, hold first clamping plate and second clamping plate simultaneously, slide out upward, then place to sample tank, let PCR tube fall into sample tank, thereby realize the effect that a row of PCR tube is quickly transferred, improve test tube transfer efficiency.

The present invention relates to a double-sided chip for nucleic acid extraction for a PCR test, a cartridge comprising same, and a nucleic acid extraction device using same. The cartridge comprising the chip for nucleic acid extraction includes: a plate-shaped body unit comprising an absorption chamber for absorbing and removing, from a sample, inhibitors which inhibit PCR performance; a front cover coupled to one side of the body unit; and a rear cover coupled to the front cover with the body unit interposed therebetween, wherein the sample passes through the absorption chamber such that a nucleic acid is separated from the sample.

The utility model relates to a PCR test assembly and system. The PCR test assembly comprises a containing plate, a sample plate, a plurality of PCR tubes and a plurality of magnetic parts. The PCR test system comprises a temperature control platform and a PCR test assembly. According to the PCR test system, after target substances in the PCR tubes are extracted through the magnetic beads, the sample plate covers the side, provided with the through holes, of the containing plate, the PCR tubes are inserted into the corresponding through holes in a one-to-one correspondence mode, a small number of residual magnetic beads in samples in the PCR tubes are adsorbed to the side walls of the PCR tubes from the bottoms of the PCR tubes through the magnetic pieces, and the target substances in the PCR tubes are extracted through the magnetic beads. The light guide piece emits a fluorescence signal to the PCR tube through the opening in the side, deviating from the sample plate, of the through hole, the fluorescence signal can fully pass through a sample in the PCR tube and generate a fluorescence signal reflected back to the light guide piece, interference is avoided, it is guaranteed that an amplification curve is normal, temperature control is conducted according to different test samples and test processes, and it is guaranteed that the test can be conducted smoothly.

This invention relates to a system and method for automatically analyzing PCR test results to identify the risk of early pancreatic cancer in individuals. The system includes a computation module, which performs the following computational processes: S1, calculating the corresponding ΔCT based on the CT values ​​of miRNAs and internal controls, and preprocessing abnormal data; S2, removing data exceeding a preset threshold and calculating the Dx value; S3, determining the positive or negative value of the Dx value based on the preset Dx judgment threshold of the selected miRNA combination to determine the sample type. The miRNA combination is a diagnostic reagent used to determine the likelihood of a subject having pancreatic cancer. The miRNA combination is selected from at least three of miR-200c / miR-154 / miR-132 / miR-130b / miR-577 / miR-30c / miR-24 / miR-23a, with one of them being miR-23a.

The utility model provides an auxiliary tool for an anti-pollution detection card, which comprises a liquid guide bin connected with the anti-pollution detection card, a PCR (Polymerase Chain Reaction) test tube arranged in the liquid guide bin and used for accommodating amplification products, and a cutting mechanism, the cutting mechanism comprises a cutting table arranged in the liquid guide bin, a cutting groove formed in the cutting table and matched with the PCR test tube, and a cutting knife mounted in the cutting groove through a floating plug. According to the utility model, the integral sealing layout is adopted, so that the problem of repeated cover opening can be effectively avoided, the possibility of pollution is reduced, and the accuracy of a test result is further ensured.

Systems and methods are disclosed for rapid PCR testing. Example embodiments may include a PCR testing module that includes a housing having a PCR machine disposed therein; a sample input station on the housing, wherein the sample input station is configured to receive a sample collection device (SCD) comprising a biological specimen sample provided by the patient; an SCD processing mechanism configured to transfer a lysed microportion of the biological specimen sample into a PCR sample tube attached to the SCD; at least one mechanism configured to separate the PCR sample tube from the SCD and transfer the PCR sample tube to the PCR machine; and a controller configured to (i) use the PCR machine to conduct a PCR test on contents of the PCR sample tube, and (ii) generate results of the PCR test.

1. Name of this design product: Integrated consumables box (PCR, TIP). 2. Purpose of this design product: Used for amplification placement of PCR test tubes, TIP heads and other consumables. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 2.

1. The name of this design product: Hollow consumables box (PCR, TIP). 2. Purpose of this design product: Used for amplification placement of PCR test tubes, TIP heads and other consumables. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The present disclosure relates to a polymerase chain reaction (PCR) test chip and a PCR test device including the same. The PCR test chip includes: a body unit made of a light-transmitting material and including an inlet through which a sample is injected; a reaction chamber including a first chamber part recessed from a lower surface of the body unit toward an upper surface of the body unit, and recessed in a first region from the lower surface, and a second chamber part in which a reagent is accommodated, being further recessed than the first chamber part toward the upper surface of the body unit with a step difference from the first region inside the first region; and an inflow path that connects the inlet to the reaction chamber and guides the sample into the reaction chamber.

The present invention relates to the field of biological detection technology, specifically to a coronavirus PCR detection kit and a method for using the kit, comprising: a storage box, on which a sealing cover is rotatably installed; a loading structure, arranged in the storage box, in which multiple groups of coronavirus PCR test reagents can be inserted; a lifting assembly, arranged in the storage box and connecting the loading structure and the sealing cover, the lifting assembly including a gap driving structure and a yaw structure, the gap driving structure can drive the loading structure to move toward the top of the storage box through the yaw structure after the sealing cover is rotated relative to the storage box by a predetermined angle; an oscillation assembly, connecting the storage box and the loading structure, the oscillation assembly can drive the loading structure to vibrate when the loading structure moves, so that during the detection process, when the sealing cover is opened, the test tube placed on the loading structure can be oscillated, thereby reducing the labor intensity of the detection personnel.

The present invention relates to a double-sided chip for nucleic acid extraction for a PCR test, a cartridge comprising same, and a nucleic acid extraction device using same. The double-sided chip for nucleic acid extraction for a PCR test includes: an input chamber into which a biological sample to be tested is inputted; an absorption chamber having, accommodated therein, an absorbent for absorbing and removing, from the sample, inhibitors which inhibit PCR performance; a nucleic acid chamber which accommodates therein a nucleic acid having passed through the absorption chamber so as to have the inhibitors removed therefrom; and a body unit which comprises the input chamber, the absorption chamber, and the nucleic acid chamber such that the sample sequentially passes through the input chamber, the absorption chamber, and the nucleic acid chamber.

The invention relates to the field of molecular biological detection, in particular to an oligonucleotide group for detecting miRNA (micro Ribonucleic Acid), which is characterized in that the specificity of real-time detection is improved through the combination of first oligonucleotide and second oligonucleotide; the two specific sequences effectively improve the detection sensitivity; the third oligonucleotide can be flexibly adjusted in a design region according to the sequence difference between miRNAs to be detected, so that the detection specificity is improved; a two-step tailing method is integrated into a one-step tailing method, so that the operation steps are simplified, and the detection time is saved; multiple mature miRNAs can be detected in one tube at the same time only through one-time PCR detection, the detection time is shortened, and the detection efficiency is improved.

The invention relates to the technical field of biological medicines, in particular to application of tri17 peptide subtillis and combination of the tri17 peptide subtillis and vancomycin in preparation of a medicine for resisting vancomycin-resistant enterococcus. The triticin has high bacteriostatic activity on vancomycin-resistant enterococcus, and the bacteriostatic activity of the triticin and vancomycin combined use is higher than that of the vancomycin with the same dosage independently used in the combined use of the triticin and vancomycin combined use of the triticin and vancomycin combined use of the triticin and vancomycin combined use. A trace broth dilution method, a chessboard method minimum inhibitory concentration test, an in-vitro bacterial growth curve and a fluorescent quantitative PCR test prove that the antibacterial activity of vancomycin is synergistically enhanced by the tripeptide tripeptide. The invention provides a novel application of triticin in reversing the drug resistance of vancomycin, which can solve the technical problems of low drug resistance and therapeutic index of clinical vancomycin and the like.

The present preservation method is a preservation method for a PCR reagent, the method includes the steps of (1) mixing all of a first composition containing DNA polymerase, a second composition containing a primer, and a third composition containing a buffer liquid to prepare a reaction liquid; and (2) refrigerating the reaction liquid, wherein the first composition, the second composition, and the third composition are accommodated in different containers.

The utility model discloses a portable small fluorescent quantitative PCR (polymerase chain reaction) instrument, which belongs to the field of medical instruments and comprises a cover body, a shell, a heating module and an amplification detection module, the cover body is movably connected with the shell, the heating module is arranged on the cover body, the amplification detection module is arranged in the shell and comprises an amplification sample block, a refrigeration sheet, a cooling sheet and a fan, and the amplification sample block is arranged in the shell. The amplification sample block is used for placing a PCR test tube, the amplification sample block and the cooling fin are detachably connected with the fan, and the refrigeration sheet is clamped between the amplification sample block and the cooling fin. The device is free of moving parts inside, small in size, light in weight, good in damping effect and convenient to carry; the requirement for a user is low, operation is easy, automatic detection is achieved when a power switch is turned on, additional operation is not needed, and the requirements of professionals and family use can be met at the same time; the heat dissipation effect is good, the heating sample block is small in size, the temperature circulation speed is high, the detection time is shortened, and rapid detection is facilitated.

A field-deployable polymerase chain reaction (PCR) test assembly enabling onsite completion of a PCR test in less than thirty minutes includes a barrel to receive a fluid. A piston is insertable into the barrel to seal the fluid within the barrel. An ultrasonic transducer is in communication with the barrel and provides transient cavitation in the fluid to release DNA or RNA from a biological sample in the fluid. A purification column is positioned within the barrel. A manifold is in fluidic communication with the barrel. A quantity of PCR reagents is positioned in a vial that is attachable to the manifold. The piston supplies pressure on the fluid to drive the fluid downwardly through the purification column and the manifold and into the vial. An optical system emits light into the vial and reads fluorescent light that indicates the presence of a target DNA within the vial.

The present invention relates to a method for carrying out end-to-end screening for cervical cancer. The method according to the invention combines two technologies, cytology and PCR on the same computer platform connected to and shared by various stakeholders. The method comprises the steps of a qualified person having access to the computer platform taking a cervical-vaginal sample using a specific sampling kit comprising a reference code to be typed into the computer platform to identify the sampled patient, then a step of carrying out, within an analysis center connected to the computer system, a cytological examination using artificial intelligence to diagnose diseased cells within the cervical-vaginal sample, and an FDA-certified HR HPV PCR test. The results of the tests are then validated by expert cytotechnologists and pathologists connected to the platform, before their ultimate storage on said computing platform. The system sends a final diagnostic report to the consulting physician via the computer platform.

The application discloses a method for rapidly screening rose germplasm resources with resistance to botrytis cinerea, which comprises the following steps of using a special primer RcWRKY22 for resistance to botrytis cinerea, inoculating botrytis cinerea, performing a fluorescent quantitative RTFQ-PCR test, and grading materials with resistance to botrytis cinerea. The method can accurately and efficiently identify rose germplasm resources with resistance to botrytis cinerea in various regions of China in less than 1 day and with a small amount of plant materials, thereby saving cost, time and labor.

The invention relates to a method for detecting a plurality of different pathogens in a sample, in which in a pre-enrichment step (1) the sample is supplied to a pre-enrichment medium for pre-enrichment, in a lysis step (2) cell walls of the pathogens in the pre-enriched sample are disrupted and thus lysates are produced, in a detection step (3) cell walls of the pathogens in the pre-enriched sample are disrupted and thus lysates are produced. The plurality of pathogens are detected by means of a multiplex PCR test, in which the pre-enrichment step (1), the cleavage step (2) and the detection step (3) are carried out in this order, and in which the cleavage is suspended without increasing the temperature prior to the detection step (3). Furthermore, a kit (100) for detecting a plurality of different pathogens is proposed.

The application provides a PCR test tube opening and closing cover device, and relates to the technical field of molecular biology. The PCR test tube opening and closing cover device comprises a base, a carrier which is slidably arranged on the base and is used for carrying a PCR test tube, a driving assembly which is arranged on the base and has a driving end connected with the carrier, and an opening and closing cover assembly which is arranged on the base and is located on a sliding path of the carrier. The opening and closing cover assembly can separate a tube cover of the PCR test tube from a tube body when the PCR test tube slides forward with the carrier, and can cover the tube cover on the tube body when the PCR test tube slides reversely with the carrier. Compared with manually opening or closing the tube cover, the PCR test tube opening and closing cover device is more labor-saving, can stably open the cover, and is not prone to spilling liquid in the tube.

The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for an infectious bursal disease virus strain, and belongs to the technical field of strain detection. The invention establishes a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method capable of identifying the Lx strain and the Lx Mut422 strain of the infectious bursal disease through one-time PCR. Comprising the following steps: designing a specific primer and a fluorescence labeling probe according to a specific gene sequence of the infectious bursal disease virus; preparing a PCR (Polymerase Chain Reaction) amplification reaction system, and carrying out fluorescent quantitative PCR amplification by taking the RNA of the sample to be detected as a template; and detecting a fluorescence signal of an amplification product, and specifically detecting the Lx strain and the Lx Mut422 strain of the infectious bursal disease. According to the detection method provided by the invention, high sensitivity of a PCR technology and accuracy of a fluorescent quantitative technology are combined, and rapid and accurate identification of the Lx strain and the Lx Mut422 strain of the infectious bursal disease can be realized.

Provided is a qPCR-based method for early sex identification of a pigeon. The method specifically comprises the following steps: S1, extracting a genomic DNA from tissue or body fluid such as pigeon feathers and saliva; S2, adjusting primers, and carrying out a PCR reaction using the extracted DNA as a template; S3, preparing pure W and Z standard sequences by means of TA cloning; S4, identifying positive clones by bacterial colony PCR; S5, adjusting primer and probe sequences; and S6, detecting specificity by means of a fluorescent quantitative PCR test. The qPCR-based method for early sex identification of a pigeon achieves good specificity and high identification accuracy by using a fluorescent quantitative PCR probe method. In this method, on the basis of the conventional fluorescent quantitative PCR probe method, dual-probe multiplex detection is further used, the two probes respectively target Z gene and W gene fragments, and the two probes are respectively labeled with different fluorescence signals, enabling simultaneous detection in one tube. One advantage is that the results are intuitive: females exhibit signals from both Z and W probes, whereas males only exhibit a signal from the Z probe.

The utility model provides a PCR tube annular support and a constant temperature nucleic acid amplification analyzer, the PCR tube annular support comprises a support body, the whole support body is in a circular ring shape, a plurality of test tube holes are distributed in the support body in a circle shape, and each test tube hole can be used for placing a PCR test tube. The PCR tube annular support solves the problems that an existing device cannot directly use linear eight-row tubes, the eight-row tubes need to be cut off for use or single tubes need to be adopted, consumables need to be sequentially put in one by one during test operation, and operation is tedious and time-consuming, reduces the number of times of taking single test tubes for multiple times, and improves test efficiency. The reagent tube is convenient to operate and reagent is convenient to add, the nucleic acid detection efficiency is improved, the cost of independently opening a circumferential eight-connecting-tube mold is also saved, and meanwhile, the foam support for mounting the PCR single tube can be independently placed on a table top and serves as a test tube rack.

A method for controlling a measurement procedure for determining a toxin produced by a toxin producer (1) on or in a climate-variable medium, in particular a body of water, wherein the measurement procedure comprises the following steps: A. Detection and / or concentration determination (101) of a constituent of a toxin producer (1) by measurement using a sensor (2); B. Sampling (102-1); C. Identification of the type of toxin producer (1) and / or the toxin gene expression by a PCR test (102-2) and / or a morphological analysis (102-3) of the toxin producer (1); and D. Detection and / or concentration determination (103) of a toxin produced by the toxin producer (1) by performing a spectroscopic measurement; wherein, based on the measurement results of the sensor (2) in step A, a decision and / or setting is made to carry out steps B and / or C and / or D.

The invention provides a microbial PCR product electrophoretic band identification method and system and electronic equipment. The method comprises the following steps: obtaining a sample electrophoresis gel map after a microbial PCR test is carried out on a pig in-vivo sample extract; segmenting the sample electrophoresis gel image based on a pre-constructed segmentation model to obtain a PCR gel image; and performing electrophoretic band identification on the PCR gel image based on a pre-constructed identification model to obtain a mark and a sample electrophoretic band, and splicing and analyzing the sample electrophoretic band and the corresponding mark to obtain length information of the sample electrophoretic band. The method can automatically identify and analyze the electrophoretic band of the microbial PCR product, so that the pathogenesis of the sick pig is quickly positioned, the medicine is timely and accurately taken, and the silence cost in the pig breeding process is reduced.