19 results about "Pcr test" patented technology

The invention relates to the technical field of antibacterial preparations, and discloses an application of etoposide in an antibacterial preparation and the antibacterial preparation, the antibacterial preparation is a biofilm inhibitor, and etoposide inhibits formation of a biofilm or destroys the formed biofilm by down-regulating gene expression of the bacterial biofilm, so that the antibacterial effect of etoposide is improved. The biofilm is a biofilm of streptococcus mutans ATCC700610, and the biofilm is a biofilm of streptococcus mutans The antibacterial preparation comprises an antibacterial drug, an oral care product, a medical coating and an anti-caries preparation. MIC and MBC determination tests, growth curve tests, crystal violet staining tests, bacterium living / death staining tests and qRT-PCR tests prove that etoposide can effectively down-regulate biofilm related gene expression (such as gtfB, gtfC, gtfD and the like), inhibit formation of a bacterial biofilm or destroy a formed biofilm, and achieve an antibacterial effect.

The invention relates to application of golden larch bark acetic acid in preparation of methicillin-resistant staphylococcus aureus (MRSA) biological membrane and bacterium holding inhibitor. A biological membrane formation inhibition experiment shows that the pseudolaric acid can remarkably inhibit formation of an MRSA biological membrane, a fluorescent quantitative PCR experiment shows that the pseudolaric acid remarkably reduces transcription of related genes of the MRSA biological membrane, and a persistent bacterium killing experiment shows that the pseudolaric acid enhances the killing capability of gentamicin on MRSA persistent bacteria. Lung epithelial cell adhesion tests find that the golden larch bark acetic acid significantly reduces the adhesion effect of the MRSA strain on epithelial cells. A test for constructing an MRSA infected greater wax moth model shows that the golden larch bark acetic acid has a system protection effect on the MRSA infected greater wax moth model.

The utility model relates to the field of testing apparatus, concretely relates to a PCR test equipment, including PCR appearance body, still including the test tube frame that can be accomodated in PCR appearance body sample tank top, be equipped with a plurality of placing groove in test tube frame, be equipped with clamping piece in a plurality of placing groove, the clamping piece includes first clamping plate and second clamping plate, first clamping plate and second clamping plate opposite side all are equipped with a plurality of arc grooves, first clamping plate and second clamping plate combination, arc groove can be used for placing PCR tube, first clamping plate and second clamping plate all can slide out upward, when using, take out test tube frame, cooperate with a plurality of arc grooves of first clamping plate and second clamping plate, place PCR tube, then adjust injection to be detected sample, adjust end after, hold first clamping plate and second clamping plate simultaneously, slide out upward, then place to sample tank, let PCR tube fall into sample tank, thereby realize the effect that a row of PCR tube is quickly transferred, improve test tube transfer efficiency.

This invention relates to a system and method for automatically analyzing PCR test results to identify the risk of early pancreatic cancer in individuals. The system includes a computation module, which performs the following computational processes: S1, calculating the corresponding ΔCT based on the CT values ​​of miRNAs and internal controls, and preprocessing abnormal data; S2, removing data exceeding a preset threshold and calculating the Dx value; S3, determining the positive or negative value of the Dx value based on the preset Dx judgment threshold of the selected miRNA combination to determine the sample type. The miRNA combination is a diagnostic reagent used to determine the likelihood of a subject having pancreatic cancer. The miRNA combination is selected from at least three of miR-200c / miR-154 / miR-132 / miR-130b / miR-577 / miR-30c / miR-24 / miR-23a, with one of them being miR-23a.

The present disclosure relates to a polymerase chain reaction (PCR) test chip and a PCR test device including the same. The PCR test chip includes: a body unit made of a light-transmitting material and including an inlet through which a sample is injected; a reaction chamber including a first chamber part recessed from a lower surface of the body unit toward an upper surface of the body unit, and recessed in a first region from the lower surface, and a second chamber part in which a reagent is accommodated, being further recessed than the first chamber part toward the upper surface of the body unit with a step difference from the first region inside the first region; and an inflow path that connects the inlet to the reaction chamber and guides the sample into the reaction chamber.

The present invention relates to the field of biological detection technology, specifically to a coronavirus PCR detection kit and a method for using the kit, comprising: a storage box, on which a sealing cover is rotatably installed; a loading structure, arranged in the storage box, in which multiple groups of coronavirus PCR test reagents can be inserted; a lifting assembly, arranged in the storage box and connecting the loading structure and the sealing cover, the lifting assembly including a gap driving structure and a yaw structure, the gap driving structure can drive the loading structure to move toward the top of the storage box through the yaw structure after the sealing cover is rotated relative to the storage box by a predetermined angle; an oscillation assembly, connecting the storage box and the loading structure, the oscillation assembly can drive the loading structure to vibrate when the loading structure moves, so that during the detection process, when the sealing cover is opened, the test tube placed on the loading structure can be oscillated, thereby reducing the labor intensity of the detection personnel.

The invention relates to the field of molecular biological detection, in particular to an oligonucleotide group for detecting miRNA (micro Ribonucleic Acid), which is characterized in that the specificity of real-time detection is improved through the combination of first oligonucleotide and second oligonucleotide; the two specific sequences effectively improve the detection sensitivity; the third oligonucleotide can be flexibly adjusted in a design region according to the sequence difference between miRNAs to be detected, so that the detection specificity is improved; a two-step tailing method is integrated into a one-step tailing method, so that the operation steps are simplified, and the detection time is saved; multiple mature miRNAs can be detected in one tube at the same time only through one-time PCR detection, the detection time is shortened, and the detection efficiency is improved.

The utility model discloses a portable small fluorescent quantitative PCR (polymerase chain reaction) instrument, which belongs to the field of medical instruments and comprises a cover body, a shell, a heating module and an amplification detection module, the cover body is movably connected with the shell, the heating module is arranged on the cover body, the amplification detection module is arranged in the shell and comprises an amplification sample block, a refrigeration sheet, a cooling sheet and a fan, and the amplification sample block is arranged in the shell. The amplification sample block is used for placing a PCR test tube, the amplification sample block and the cooling fin are detachably connected with the fan, and the refrigeration sheet is clamped between the amplification sample block and the cooling fin. The device is free of moving parts inside, small in size, light in weight, good in damping effect and convenient to carry; the requirement for a user is low, operation is easy, automatic detection is achieved when a power switch is turned on, additional operation is not needed, and the requirements of professionals and family use can be met at the same time; the heat dissipation effect is good, the heating sample block is small in size, the temperature circulation speed is high, the detection time is shortened, and rapid detection is facilitated.

A field-deployable polymerase chain reaction (PCR) test assembly enabling onsite completion of a PCR test in less than thirty minutes includes a barrel to receive a fluid. A piston is insertable into the barrel to seal the fluid within the barrel. An ultrasonic transducer is in communication with the barrel and provides transient cavitation in the fluid to release DNA or RNA from a biological sample in the fluid. A purification column is positioned within the barrel. A manifold is in fluidic communication with the barrel. A quantity of PCR reagents is positioned in a vial that is attachable to the manifold. The piston supplies pressure on the fluid to drive the fluid downwardly through the purification column and the manifold and into the vial. An optical system emits light into the vial and reads fluorescent light that indicates the presence of a target DNA within the vial.

The application discloses a method for rapidly screening rose germplasm resources with resistance to botrytis cinerea, which comprises the following steps of using a special primer RcWRKY22 for resistance to botrytis cinerea, inoculating botrytis cinerea, performing a fluorescent quantitative RTFQ-PCR test, and grading materials with resistance to botrytis cinerea. The method can accurately and efficiently identify rose germplasm resources with resistance to botrytis cinerea in various regions of China in less than 1 day and with a small amount of plant materials, thereby saving cost, time and labor.

The application provides a PCR test tube opening and closing cover device, and relates to the technical field of molecular biology. The PCR test tube opening and closing cover device comprises a base, a carrier which is slidably arranged on the base and is used for carrying a PCR test tube, a driving assembly which is arranged on the base and has a driving end connected with the carrier, and an opening and closing cover assembly which is arranged on the base and is located on a sliding path of the carrier. The opening and closing cover assembly can separate a tube cover of the PCR test tube from a tube body when the PCR test tube slides forward with the carrier, and can cover the tube cover on the tube body when the PCR test tube slides reversely with the carrier. Compared with manually opening or closing the tube cover, the PCR test tube opening and closing cover device is more labor-saving, can stably open the cover, and is not prone to spilling liquid in the tube.

The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for an infectious bursal disease virus strain, and belongs to the technical field of strain detection. The invention establishes a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method capable of identifying the Lx strain and the Lx Mut422 strain of the infectious bursal disease through one-time PCR. Comprising the following steps: designing a specific primer and a fluorescence labeling probe according to a specific gene sequence of the infectious bursal disease virus; preparing a PCR (Polymerase Chain Reaction) amplification reaction system, and carrying out fluorescent quantitative PCR amplification by taking the RNA of the sample to be detected as a template; and detecting a fluorescence signal of an amplification product, and specifically detecting the Lx strain and the Lx Mut422 strain of the infectious bursal disease. According to the detection method provided by the invention, high sensitivity of a PCR technology and accuracy of a fluorescent quantitative technology are combined, and rapid and accurate identification of the Lx strain and the Lx Mut422 strain of the infectious bursal disease can be realized.

Provided is a qPCR-based method for early sex identification of a pigeon. The method specifically comprises the following steps: S1, extracting a genomic DNA from tissue or body fluid such as pigeon feathers and saliva; S2, adjusting primers, and carrying out a PCR reaction using the extracted DNA as a template; S3, preparing pure W and Z standard sequences by means of TA cloning; S4, identifying positive clones by bacterial colony PCR; S5, adjusting primer and probe sequences; and S6, detecting specificity by means of a fluorescent quantitative PCR test. The qPCR-based method for early sex identification of a pigeon achieves good specificity and high identification accuracy by using a fluorescent quantitative PCR probe method. In this method, on the basis of the conventional fluorescent quantitative PCR probe method, dual-probe multiplex detection is further used, the two probes respectively target Z gene and W gene fragments, and the two probes are respectively labeled with different fluorescence signals, enabling simultaneous detection in one tube. One advantage is that the results are intuitive: females exhibit signals from both Z and W probes, whereas males only exhibit a signal from the Z probe.

A method for controlling a measurement procedure for determining a toxin produced by a toxin producer (1) on or in a climate-variable medium, in particular a body of water, wherein the measurement procedure comprises the following steps: A. Detection and / or concentration determination (101) of a constituent of a toxin producer (1) by measurement using a sensor (2); B. Sampling (102-1); C. Identification of the type of toxin producer (1) and / or the toxin gene expression by a PCR test (102-2) and / or a morphological analysis (102-3) of the toxin producer (1); and D. Detection and / or concentration determination (103) of a toxin produced by the toxin producer (1) by performing a spectroscopic measurement; wherein, based on the measurement results of the sensor (2) in step A, a decision and / or setting is made to carry out steps B and / or C and / or D.

The invention provides a microbial PCR product electrophoretic band identification method and system and electronic equipment. The method comprises the following steps: obtaining a sample electrophoresis gel map after a microbial PCR test is carried out on a pig in-vivo sample extract; segmenting the sample electrophoresis gel image based on a pre-constructed segmentation model to obtain a PCR gel image; and performing electrophoretic band identification on the PCR gel image based on a pre-constructed identification model to obtain a mark and a sample electrophoretic band, and splicing and analyzing the sample electrophoretic band and the corresponding mark to obtain length information of the sample electrophoretic band. The method can automatically identify and analyze the electrophoretic band of the microbial PCR product, so that the pathogenesis of the sick pig is quickly positioned, the medicine is timely and accurately taken, and the silence cost in the pig breeding process is reduced.

The utility model discloses a PCR (Polymerase Chain Reaction) detector and belongs to the technical field of biological and medical detection. The device is used for PCR test tube detection, in the device, an upper plate is arranged above a detection module, a bracket in a pressing plate assembly is clamped in a first through hole of the upper plate, a support connected in the bracket is connected with a pressing plate, a second through hole is formed in a supporting platform, and the upper plate comprises a rotating shaft fixing seat and a torsional spring groove; a rotating shaft on the pressing plate is fixed to the upper plate through a rotating shaft fixing base, one end of one torsional spring rotating arm is clamped in the torsional spring groove, and one end of the other torsional spring rotating arm is clamped in the pressing plate. The test tube body enters the test tube groove through the second through hole in the supporting platform, the test tube cover is clamped on the supporting platform, the pressing plate transmits pressing force to the test tube cover through the supporting platform, so that the PCR detection test tube is driven to pop up, the torsional spring provides elastic force for the pressing plate, and the pressing plate is restored to the original position after the PCR detection test tube is taken out. The effect of quickly and simply taking out the PCR detection test tube is achieved.

An antibody-antigen capture ELISA-PCR test set for detection of a target protein comprises a first antibody-DNA conjugate having a first antibody which is configured to bind to a target protein; and a first DNA oligo linked to the first antibody; a second antibody-DNA conjugate having a second antibody which is configured to bind to the target protein; and a second DNA oligo linked to the second antibody; and multiple immobilized DNA fragments attached to a designated surface on their attaching ends, wherein the immobilized DNA fragments are complementary to one of the first DNA oligo and the second DNA oligo.

The present invention belongs to the field of virus storage technology, and in particular, a temporary storage device for bovine reovirus RT-PCR testing, comprising a storage tank temporarily used to store bovine reovirus samples and a reagent tube containing the bovine reovirus samples, wherein the interior of the storage tank is provided with a driving cavity and an action cavity from bottom to top, respectively, the inner bottom wall of the action cavity is in sliding contact with a support base plate, a support column is fixedly sleeved on the surface at the center of the support base plate, a rotating mechanism is provided on the outer surface of the support column, the rotating mechanism is connected to a shaking mechanism, and a height adjustment mechanism is provided inside the driving cavity. The temporary storage device for bovine reovirus RT-PCR testing drives the driving bevel gear to rotate around the center of the support column when the driving ring rotates, and because the driving bevel gear is meshed with the outer surface of the linkage bevel gear, the driving bevel gear can drive the linkage shaft to rotate when the driving bevel gear rotates, thereby driving the cam rod to rotate and realizing the up and down movement of the traction rod.

The present disclosure provides methods and systems for the massively-parallel detection of pathogens, such as SARS-CoV-2 virus, in a set of multiple samples via PCR test. Various implementations may provide for barcode / primer sequences that are designed to allow for a large number of samples and / or multiple pathogens to be analyzed in a single test. Included within the scope hereof are methods and systems for performing tests of multiple samples at once, via a one-pot test protocol, as well as methods and systems for designing test parameters (such as barcode / primer sequences) in a manner that allows for parallel testing.