10 results about "Pcr test" patented technology

The invention relates to application of golden larch bark acetic acid in preparation of methicillin-resistant staphylococcus aureus (MRSA) biological membrane and bacterium holding inhibitor. A biological membrane formation inhibition experiment shows that the pseudolaric acid can remarkably inhibit formation of an MRSA biological membrane, a fluorescent quantitative PCR experiment shows that the pseudolaric acid remarkably reduces transcription of related genes of the MRSA biological membrane, and a persistent bacterium killing experiment shows that the pseudolaric acid enhances the killing capability of gentamicin on MRSA persistent bacteria. Lung epithelial cell adhesion tests find that the golden larch bark acetic acid significantly reduces the adhesion effect of the MRSA strain on epithelial cells. A test for constructing an MRSA infected greater wax moth model shows that the golden larch bark acetic acid has a system protection effect on the MRSA infected greater wax moth model.

The utility model relates to the field of testing apparatus, concretely relates to a PCR test equipment, including PCR appearance body, still including the test tube frame that can be accomodated in PCR appearance body sample tank top, be equipped with a plurality of placing groove in test tube frame, be equipped with clamping piece in a plurality of placing groove, the clamping piece includes first clamping plate and second clamping plate, first clamping plate and second clamping plate opposite side all are equipped with a plurality of arc grooves, first clamping plate and second clamping plate combination, arc groove can be used for placing PCR tube, first clamping plate and second clamping plate all can slide out upward, when using, take out test tube frame, cooperate with a plurality of arc grooves of first clamping plate and second clamping plate, place PCR tube, then adjust injection to be detected sample, adjust end after, hold first clamping plate and second clamping plate simultaneously, slide out upward, then place to sample tank, let PCR tube fall into sample tank, thereby realize the effect that a row of PCR tube is quickly transferred, improve test tube transfer efficiency.

This invention relates to a system and method for automatically analyzing PCR test results to identify the risk of early pancreatic cancer in individuals. The system includes a computation module, which performs the following computational processes: S1, calculating the corresponding ΔCT based on the CT values ​​of miRNAs and internal controls, and preprocessing abnormal data; S2, removing data exceeding a preset threshold and calculating the Dx value; S3, determining the positive or negative value of the Dx value based on the preset Dx judgment threshold of the selected miRNA combination to determine the sample type. The miRNA combination is a diagnostic reagent used to determine the likelihood of a subject having pancreatic cancer. The miRNA combination is selected from at least three of miR-200c / miR-154 / miR-132 / miR-130b / miR-577 / miR-30c / miR-24 / miR-23a, with one of them being miR-23a.

The present disclosure relates to a polymerase chain reaction (PCR) test chip and a PCR test device including the same. The PCR test chip includes: a body unit made of a light-transmitting material and including an inlet through which a sample is injected; a reaction chamber including a first chamber part recessed from a lower surface of the body unit toward an upper surface of the body unit, and recessed in a first region from the lower surface, and a second chamber part in which a reagent is accommodated, being further recessed than the first chamber part toward the upper surface of the body unit with a step difference from the first region inside the first region; and an inflow path that connects the inlet to the reaction chamber and guides the sample into the reaction chamber.

The application provides a PCR test tube opening and closing cover device, and relates to the technical field of molecular biology. The PCR test tube opening and closing cover device comprises a base, a carrier which is slidably arranged on the base and is used for carrying a PCR test tube, a driving assembly which is arranged on the base and has a driving end connected with the carrier, and an opening and closing cover assembly which is arranged on the base and is located on a sliding path of the carrier. The opening and closing cover assembly can separate a tube cover of the PCR test tube from a tube body when the PCR test tube slides forward with the carrier, and can cover the tube cover on the tube body when the PCR test tube slides reversely with the carrier. Compared with manually opening or closing the tube cover, the PCR test tube opening and closing cover device is more labor-saving, can stably open the cover, and is not prone to spilling liquid in the tube.

The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for an infectious bursal disease virus strain, and belongs to the technical field of strain detection. The invention establishes a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method capable of identifying the Lx strain and the Lx Mut422 strain of the infectious bursal disease through one-time PCR. Comprising the following steps: designing a specific primer and a fluorescence labeling probe according to a specific gene sequence of the infectious bursal disease virus; preparing a PCR (Polymerase Chain Reaction) amplification reaction system, and carrying out fluorescent quantitative PCR amplification by taking the RNA of the sample to be detected as a template; and detecting a fluorescence signal of an amplification product, and specifically detecting the Lx strain and the Lx Mut422 strain of the infectious bursal disease. According to the detection method provided by the invention, high sensitivity of a PCR technology and accuracy of a fluorescent quantitative technology are combined, and rapid and accurate identification of the Lx strain and the Lx Mut422 strain of the infectious bursal disease can be realized.

A method for controlling a measurement procedure for determining a toxin produced by a toxin producer (1) on or in a climate-variable medium, in particular a body of water, wherein the measurement procedure comprises the following steps: A. Detection and / or concentration determination (101) of a constituent of a toxin producer (1) by measurement using a sensor (2); B. Sampling (102-1); C. Identification of the type of toxin producer (1) and / or the toxin gene expression by a PCR test (102-2) and / or a morphological analysis (102-3) of the toxin producer (1); and D. Detection and / or concentration determination (103) of a toxin produced by the toxin producer (1) by performing a spectroscopic measurement; wherein, based on the measurement results of the sensor (2) in step A, a decision and / or setting is made to carry out steps B and / or C and / or D.

The invention provides a microbial PCR product electrophoretic band identification method and system and electronic equipment. The method comprises the following steps: obtaining a sample electrophoresis gel map after a microbial PCR test is carried out on a pig in-vivo sample extract; segmenting the sample electrophoresis gel image based on a pre-constructed segmentation model to obtain a PCR gel image; and performing electrophoretic band identification on the PCR gel image based on a pre-constructed identification model to obtain a mark and a sample electrophoretic band, and splicing and analyzing the sample electrophoretic band and the corresponding mark to obtain length information of the sample electrophoretic band. The method can automatically identify and analyze the electrophoretic band of the microbial PCR product, so that the pathogenesis of the sick pig is quickly positioned, the medicine is timely and accurately taken, and the silence cost in the pig breeding process is reduced.

The utility model discloses a PCR (Polymerase Chain Reaction) detector and belongs to the technical field of biological and medical detection. The device is used for PCR test tube detection, in the device, an upper plate is arranged above a detection module, a bracket in a pressing plate assembly is clamped in a first through hole of the upper plate, a support connected in the bracket is connected with a pressing plate, a second through hole is formed in a supporting platform, and the upper plate comprises a rotating shaft fixing seat and a torsional spring groove; a rotating shaft on the pressing plate is fixed to the upper plate through a rotating shaft fixing base, one end of one torsional spring rotating arm is clamped in the torsional spring groove, and one end of the other torsional spring rotating arm is clamped in the pressing plate. The test tube body enters the test tube groove through the second through hole in the supporting platform, the test tube cover is clamped on the supporting platform, the pressing plate transmits pressing force to the test tube cover through the supporting platform, so that the PCR detection test tube is driven to pop up, the torsional spring provides elastic force for the pressing plate, and the pressing plate is restored to the original position after the PCR detection test tube is taken out. The effect of quickly and simply taking out the PCR detection test tube is achieved.

An antibody-antigen capture ELISA-PCR test set for detection of a target protein comprises a first antibody-DNA conjugate having a first antibody which is configured to bind to a target protein; and a first DNA oligo linked to the first antibody; a second antibody-DNA conjugate having a second antibody which is configured to bind to the target protein; and a second DNA oligo linked to the second antibody; and multiple immobilized DNA fragments attached to a designated surface on their attaching ends, wherein the immobilized DNA fragments are complementary to one of the first DNA oligo and the second DNA oligo.