Method for detecting vibrio parahaemolyticus PCR added with amplification internal sign

Abstract

The invention relates to a PCR test method of a vibrio parahaemolyticus with internal amplification control in the food safety technology filed, which comprises steps as follows: firstly the construction and synthesis of the internal amplification control sequence artificially and design of the specific primers; secondly the PCR reaction system is laid in the best reaction condition; thirdly PRC is amplified; fourthly DNA of the different-serotype vibrio parahaemolyticus and different-species non vibrio parahaemolyticus is extracted for PRC test to identify the specific primers and confirm whether the internal amplification control can be normally amplified in the non vibrio parahaemolyticus sample test; finally the evaluation of the built PCR reaction through the artificial and natural pollution samples tests. The invention shows the occurrence of the false negative in the testing process and improves the accuracy rate. The invention provides efficient and reliable technical means to satisfy the urgent need of the foodborne patheogen investigation and test in the quarantine law enforcement.

Description

technical field

[0001] The invention relates to a detection method in the technical field of food safety, in particular to a PCR detection method for Vibrio parahaemolyticus added with an amplified internal standard. Background technique

[0002] PCR, the abbreviation of polymerase chain reaction in English, refers to: polymerase chain reaction. Vibrio parahaemolyticus (Vibrio parahaemolyticus) is a halophilic bacterium belonging to the genus Vibrio in the Vibrio family. It is Gram-negative and does not produce spores. In seawater, seabed sediments and marine organisms such as fish and shellfish. It is an important pathogen causing foodborne diseases. Poisoning will occur when people eat seafood such as crabs, oysters, shrimps, shellfish, etc. that are contaminated with the bacteria and have not been properly processed. Data from the National Foodborne Disease Surveillance Network shows that since 1998, in the monitored provinces and cities, the occurrence scale of Vibrio...

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