Rotavirus RT PCR detecting kit and its detecting method

Abstract

Thisa invention relates to a semi-set RT-PCR test reagent box for testing and verifying wheel-like virus including a MLV inverse transcriptase, Rnasin, DNA poly-enzyme, primers, dNTP, MgCl2 and 10 x buffer solution, in which, the upstream primer P1 is 5'-GTA TGG TAT TGA ATA TAC CAC-3', the downstream primer P2 is 5'-GAT CCT GTT GGC CAT CC-3. The RT-PCR test method includes: picking up a due test sample template, adding MLV inverse transcriptases, Rnasin, DNA poly-enzymes, primers, dNTP, MgCl2, 10 x buffer solution, the due test sample template and DECP water to be mixed uniformly to be enlarged on a PRC instrument, then the product is electrophoresis-enlarged in a device to get a result, which is recorded, analyzed and judged.

Description

[Affiliated technical field] [0001] The invention relates to a RT-PCR detection kit for detecting and identifying rotavirus and a use method thereof, belonging to the field of biotechnology. [Background technique] [0002] Human rotaviruses belong to the genus Rotavirus in the Reoviridae family. There are three groups of rotaviruses that can cause diarrhea in humans: A, B, and C. Among them, group A is infantile diarrhea rotavirus, group B and C are adult rotavirus. No matter in developed countries or developing countries, diarrhea caused by group A rotavirus has a relatively high incidence rate, and is the most important cause of morbidity and death in infants and young children. In my country, the situation is even more serious. Diarrhea caused by rotavirus and adult diarrhea are very prevalent. According to the statistics of WHO in 1997, there are 1.4 billion rotavirus infections in the world every year, and 870,000 people in developing countries die from it. There are...

AI Technical Summary

Problems solved by technology

The current food hygiene standards cannot explain the contamination of viruses in food. Although viruses are strictly intracellular parasites and cannot reproduce in food, a small amount of viruses in food can cause disease

According to foreign data, there are considerable numbers and types of foodborne viruses and the diseases they cause, some of which are widespread, highly contagious, prone to outbreaks, and serious hazards. This is not only a danger in the field of food hygiene. Factors, a major threat to people's public health and nutritional health, but also a serious loss to the food industry and the national economy

[0005] Rotavirus is an important food-borne virus. Conventional detection methods for viruses in food include electron microscope observation, cell culture, nucleic acid hybridization, ELISA and polymerase chain reaction, but electron microscope observation, nucleic acid hybridization and enzyme-linked immunosorbent The sensitivity of the detection method is relatively low, and it cannot be used alone for detection; the operation of the cell culture method is cumbersome and takes a long time, and it usually takes a week to observe the cytopathic reaction. Therefore, the rapid development, high sensitivity and easy operation of molecules Biological detection methods and their kits are of great significance for the detection and prevention of foodborne rotavirus

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