Quantitative fluorescent multiplex PCR test kit for Epstein-Barr virus, and application thereof in nasopharyngeal carcinoma screening

A technology of multiple fluorescent quantification and detection kits, applied in the direction of microbial measurement/testing, fluorescence/phosphorescence, biochemical equipment and methods, etc., to achieve accurate prediction of disease risk, broad market, and high sensitivity

Inactive Publication Date: 2012-12-26
广州市第十二人民医院
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Problems solved by technology

However, the multiplex fluorescent quantitative PCR detection kit for the mutation of the key target gene of E

Method used

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  • Quantitative fluorescent multiplex PCR test kit for Epstein-Barr virus, and application thereof in nasopharyngeal carcinoma screening
  • Quantitative fluorescent multiplex PCR test kit for Epstein-Barr virus, and application thereof in nasopharyngeal carcinoma screening
  • Quantitative fluorescent multiplex PCR test kit for Epstein-Barr virus, and application thereof in nasopharyngeal carcinoma screening

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Embodiment 1

[0035] 1. Oncogenes and mutation types detected:

[0036] The occurrence of NPC caused by EBV is related to some latent antigens of EBV, such as latent membrane protein (LMP) and EBV associated nuclear antigen-1 (EBNA-1). A large number of studies have focused on the analysis of these virus gene mutations in NPC patients. For example, the Affiliated Cancer Hospital of Sun Yat-Sen University surveyed 312,680 people in 17 towns in Sihui City, and found 60 NPC patients; and conducted the full sequence of EBV in South China for the first time at home and abroad. Assay work found that EBNA gene variants are associated with nasopharyngeal carcinoma. The project team found that only valine (V-val) EBNA1 (detection rate 98.1%) exists in nasopharyngeal carcinoma tissues, while EBNA1 V-val can be detected in peripheral blood lymphocytes and saliva. Both alanine (P-ala) and threonine (P-thr) EBNA1 were detected. And found that in peripheral blood lymphocytes and saliva, almost all V-va...

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Abstract

The invention relates to a quantitative fluorescent multiplex PCR test kit for Epstein-Barr virus, and the kit comprises the following reagents: a, specific primer pairs for amplifying mutations of key target genes in the Epstein-Barr virus; and b, specific probes for the mutations of key target genes in the Epstein-Barr virus, wherein the mutations are P-thr, V-val and V-leu in the coding region of EBNA-1 gene, promoters Cp, Fp and Zp of the EBNA-1 gene, and XhoI-loss, 30bp Deletion and Ser 366 Thr of LMP1 gene. The test kit is advantageous in that: the aim of precise prediction of the risk of nasopharyngeal carcinoma in high risk population can be achieved; the main carcinogenic mutations can be well known for the combination of population screening and population intervention for nasopharyngeal carcinoma; and the kit has the advantages of high in sensitivity, fast in test, and low in cost, having broad market prospect.

Description

technical field [0001] The invention relates to a detection kit and application thereof, in particular to a multiple fluorescence quantitative PCR detection kit for Epstein-Barr virus and its application in nasopharyngeal carcinoma screening. Background technique [0002] Nasopharyngeal carcinoma (NPC) is a common malignant tumor in southern my country and Southeast Asia. It develops progressively and often metastasizes and spreads later. At present, nasopharyngeal carcinoma mainly relies on radiotherapy, and the prognosis of patients with advanced tumors is still very poor. The 5-year survival rate of stage I patients after radiotherapy can reach 90%, and the 5-year survival rate of stage IV patients is only 30%. Therefore, early detection is the key to improving the cure rate. key to rate. Serological screening for high-risk groups of nasopharyngeal carcinoma is an important way to detect early nasopharyngeal carcinoma. [0003] Epstein-Barr virus (EBV) is the most impor...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
Inventor 刘斌
Owner 广州市第十二人民医院
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