7 results about "Molecular epidemiology" patented technology

The invention provides a fluorescent quantitative PCR (polymerase chain reaction) detection primer, a kit and a method for a novel variable chalmavirus VP gene, and belongs to the technical field of virus detection. A specific primer is designed and synthesized according to a conserved sequence of a VP gene of the novel variant chalmavirus, a recombinant plasmid is constructed, then the recombinant plasmid is used as a standard substance to establish a standard curve, a real-time fluorescent quantitative PCR detection system aiming at the novel variant chalmavirus is constructed, and specific detection of the novel variant chalmavirus is realized. The detection method can be used for quantitatively detecting the novel variant chalmavirus in the to-be-detected sample, and has the characteristics of high speed, high sensitivity, strong specificity and the like; the method provides a key technical support for molecular epidemiological investigation of the Sepamavirus in animal populations, is helpful for realizing early screening of infected individuals and timely blocking of virus transmission, and has important practical significance for guaranteeing population health and maintaining high mountain ecosystem stability.

The invention relates to the technical field of molecular biology, and discloses a primer probe set for species identification of echinococcus granulosus, and the primer probe set comprises a first primer probe set for targeting echinococcus granulosus, a second primer probe set for targeting echinococcus multilocularis and / or a third primer probe set for targeting echinococcus stone. According to the primer probe group, synchronous, rapid and high-specificity identification and detection of three kinds of echinococcus, namely echinococcus granulosus (Eg), echinococcus multilocularis (Em) and echinococcus stone (Es), are realized. When the primer probe group is used for identifying and detecting the three kinds of echinococcus, the detection sensitivity reaches 200 copies / mL, and the positive coincidence rate reaches 100%. The primer probe group realizes synchronous and accurate identification of echinococcus species, and has a wide application prospect in molecular epidemiological investigation and port quarantine.

This invention relates to a primer set and probe set for dual real-time quantitative PCR detection of GAstV-1 and GAstV-2. The sequences of the primer set are shown in SEQ ID NO. 1-3, and the sequences of the probe set are shown in SEQ ID NO. 4-5. This invention provides a primer set, probe set, and kit for dual real-time quantitative PCR detection of GAstV-1 and GAstV-2, and establishes a detection method capable of simultaneously detecting GAstV-1 and GAstV-2. This method has advantages such as saving primer quantity, reducing detection costs, simultaneous detection, rapid detection, high efficiency, accurate quantification, high sensitivity, strong specificity, and good reproducibility. It lays the foundation for conducting molecular epidemiological surveys of GAstV-1 and GAstV-2 and subsequent scientific prevention and control of related diseases, and has significant research value.

The application provides a general avian infectious bronchitis virus whole genome deep sequencing method and application. The method realizes efficient amplification of long fragments of whole genomes of different genotype IBV epidemic strains by designing and applying a set of combinations containing 24 pairs of specific primers. The specific sequences of the primer combinations are shown in SEQ NO:1 to SEQ NO:48. Compared with a traditional sequencing method, the application significantly reduces the sequencing cost. Only two PCR reactions are needed, and more than 27 kb of IBV full-length genome sequence can be successfully obtained, which provides a more economical and efficient solution for IBV related scientific research and molecular epidemiology monitoring, and helps to promote the development of related field scientific research.

The invention relates to the technical field of molecular biological detection, and particularly discloses a sheep pasteurella serum A type, B type and F type triple PCR detection method, a kit and application thereof. According to the method, three pairs of specific primers are respectively designed for serum A type, B type and F type sheep pasteurella, extracted sheep pasteurella DNA is taken as a template, a PCR amplification system is constructed by the specific primers for triple PCR amplification, negative and positive control is provided in detection, single detection of pasteurella serum A type, B type and F type can be rapidly and accurately detected, and detection of the serum A type, B type and F type of pasteurella serum B type and F type of pasteurella serum B type and F type of pasteurella serum B type and F type of pasteurella serum A type, B type and F type of pasteurella serum B type and F type of pasteurella serum B type can be rapidly and accurately detected. The B type and F type can be simultaneously detected, the accuracy of serum typing is greatly improved, the occurrence probability of false positive is reduced, the specificity is relatively strong, and the kit can be used for pasteurella serotype general survey, molecular epidemiological investigation and vaccine serotype screening and detection.

The application discloses a primer set for amplifying a whole genome sequence of maize chlorotic mottle virus and application thereof. The primer set comprises 10 primer pairs. By using each primer pair in the primer set, one-step RT-PCR amplification and sequencing are carried out on a maize chlorotic mottle virus or a sample containing the maize chlorotic mottle virus as a template, and comparison analysis and sequence splicing are carried out on the sequencing result, so that the whole genome sequence of the maize chlorotic mottle virus can be obtained. The primer set provided by the application can be used for detection and identification of the maize chlorotic mottle virus, is simple in operation method, and is accurate and reliable in result. The application not only has guiding significance for rapid and accurate detection and identification of the maize chlorotic mottle virus in China and scientific prevention and control of maize lethal necrosis disease, but also provides a powerful tool for evolution analysis and molecular epidemiology research of the maize chlorotic mottle virus.

The application discloses a primer group, a kit, a typing method and application for rapid typing of porcine delta coronavirus, and relates to the technical field of virus genome detection. The primer group comprises an upstream primer PDCoV-F, wherein the nucleotide sequence of the primer is shown as SEQ ID NO:1; and a downstream primer PDCoV-R, wherein the nucleotide sequence of the primer is shown as SEQ ID NO:2. The primer group is used for amplifying a core genome fragment obtained through screening, the fragment can represent the evolutionary topology structure of the whole genome, the typing resolution is higher than that of a traditional single gene method, and information distortion is avoided; the fragment is short in length, only needs conventional PCR and Sanger sequencing, the cost is reduced by more than 80%, data analysis is simple, and a professional biological information platform is not needed; an operation process is standardized, the flux is high, and the application is suitable for large-scale molecular epidemiological monitoring in a clinic; and the typing result can reflect the virus evolution history and the transmission path, and can provide a reliable and scientific basis for epidemic prevention and control.