15 results about "Molecular epidemiology" patented technology

The invention provides a whole genome capture kit suitable for four types of dengue virus and application, the whole genome capture kit comprises two groups of primer pools, primers in the first primer pool comprise targeting primers with primer sequences shown as SEQ ID NO.1-SEQ ID NO.22, primers in the second primer pool comprise targeting primers with primer sequences shown as SEQ ID NO.22, and primers in the third primer pool comprise targeting primers with primer sequences shown as SEQ ID NO.22; a primer in the second primer pool contains a target primer of which the primer sequence is shown as SEQ ID NO.41. Reverse transcription and enrichment amplification of the dengue virus whole genome can be carried out in nucleic acid samples extracted from blood, throat swab and infected tissue environments, and the dengue virus detection kit can be used for detecting and identifying the dengue virus. And a powerful technical means can be provided for evolution analysis, molecular epidemiological research and scientific prevention and control of the dengue virus.

The invention provides a fluorescent quantitative PCR (polymerase chain reaction) detection primer, a kit and a method for a novel variable chalmavirus VP gene, and belongs to the technical field of virus detection. A specific primer is designed and synthesized according to a conserved sequence of a VP gene of the novel variant chalmavirus, a recombinant plasmid is constructed, then the recombinant plasmid is used as a standard substance to establish a standard curve, a real-time fluorescent quantitative PCR detection system aiming at the novel variant chalmavirus is constructed, and specific detection of the novel variant chalmavirus is realized. The detection method can be used for quantitatively detecting the novel variant chalmavirus in the to-be-detected sample, and has the characteristics of high speed, high sensitivity, strong specificity and the like; the method provides a key technical support for molecular epidemiological investigation of the Sepamavirus in animal populations, is helpful for realizing early screening of infected individuals and timely blocking of virus transmission, and has important practical significance for guaranteeing population health and maintaining high mountain ecosystem stability.

The invention relates to the technical field of molecular biology, and discloses a primer probe set for species identification of echinococcus granulosus, and the primer probe set comprises a first primer probe set for targeting echinococcus granulosus, a second primer probe set for targeting echinococcus multilocularis and / or a third primer probe set for targeting echinococcus stone. According to the primer probe group, synchronous, rapid and high-specificity identification and detection of three kinds of echinococcus, namely echinococcus granulosus (Eg), echinococcus multilocularis (Em) and echinococcus stone (Es), are realized. When the primer probe group is used for identifying and detecting the three kinds of echinococcus, the detection sensitivity reaches 200 copies / mL, and the positive coincidence rate reaches 100%. The primer probe group realizes synchronous and accurate identification of echinococcus species, and has a wide application prospect in molecular epidemiological investigation and port quarantine.

This invention relates to a primer set and probe set for dual real-time quantitative PCR detection of GAstV-1 and GAstV-2. The sequences of the primer set are shown in SEQ ID NO. 1-3, and the sequences of the probe set are shown in SEQ ID NO. 4-5. This invention provides a primer set, probe set, and kit for dual real-time quantitative PCR detection of GAstV-1 and GAstV-2, and establishes a detection method capable of simultaneously detecting GAstV-1 and GAstV-2. This method has advantages such as saving primer quantity, reducing detection costs, simultaneous detection, rapid detection, high efficiency, accurate quantification, high sensitivity, strong specificity, and good reproducibility. It lays the foundation for conducting molecular epidemiological surveys of GAstV-1 and GAstV-2 and subsequent scientific prevention and control of related diseases, and has significant research value.

The present application relates to a kind of primer set and probe set for chicken circle virus AGV2 and GyV7 dual real-time fluorescent quantitative PCR detection, the sequence of the primer set and probe set is as shown in SEQ ID NO.1-6, the present application provides primer set and probe set and kit for chicken circle virus AGV2 and GyV7 dual real-time fluorescent quantitative PCR detection, and establishes the detection method capable of simultaneously detecting chicken circle virus AGV2 and GyV7, with the advantages of simultaneous detection, rapid detection, high efficiency, quantitative accuracy, high sensitivity, strong specificity, good repeatability, for chicken population to carry out new chicken circle virus AGV2 and GyV7 molecular epidemiology survey and subsequent scientific prevention and control related disease lay foundation, with very important research significance.

The application provides a general avian infectious bronchitis virus whole genome deep sequencing method and application. The method realizes efficient amplification of long fragments of whole genomes of different genotype IBV epidemic strains by designing and applying a set of combinations containing 24 pairs of specific primers. The specific sequences of the primer combinations are shown in SEQ NO:1 to SEQ NO:48. Compared with a traditional sequencing method, the application significantly reduces the sequencing cost. Only two PCR reactions are needed, and more than 27 kb of IBV full-length genome sequence can be successfully obtained, which provides a more economical and efficient solution for IBV related scientific research and molecular epidemiology monitoring, and helps to promote the development of related field scientific research.

The invention relates to the technical field of molecular biological detection, and particularly discloses a sheep pasteurella serum A type, B type and F type triple PCR detection method, a kit and application thereof. According to the method, three pairs of specific primers are respectively designed for serum A type, B type and F type sheep pasteurella, extracted sheep pasteurella DNA is taken as a template, a PCR amplification system is constructed by the specific primers for triple PCR amplification, negative and positive control is provided in detection, single detection of pasteurella serum A type, B type and F type can be rapidly and accurately detected, and detection of the serum A type, B type and F type of pasteurella serum B type and F type of pasteurella serum B type and F type of pasteurella serum B type and F type of pasteurella serum A type, B type and F type of pasteurella serum B type and F type of pasteurella serum B type can be rapidly and accurately detected. The B type and F type can be simultaneously detected, the accuracy of serum typing is greatly improved, the occurrence probability of false positive is reduced, the specificity is relatively strong, and the kit can be used for pasteurella serotype general survey, molecular epidemiological investigation and vaccine serotype screening and detection.

The application discloses a primer set for amplifying a whole genome sequence of maize chlorotic mottle virus and application thereof. The primer set comprises 10 primer pairs. By using each primer pair in the primer set, one-step RT-PCR amplification and sequencing are carried out on a maize chlorotic mottle virus or a sample containing the maize chlorotic mottle virus as a template, and comparison analysis and sequence splicing are carried out on the sequencing result, so that the whole genome sequence of the maize chlorotic mottle virus can be obtained. The primer set provided by the application can be used for detection and identification of the maize chlorotic mottle virus, is simple in operation method, and is accurate and reliable in result. The application not only has guiding significance for rapid and accurate detection and identification of the maize chlorotic mottle virus in China and scientific prevention and control of maize lethal necrosis disease, but also provides a powerful tool for evolution analysis and molecular epidemiology research of the maize chlorotic mottle virus.

This invention discloses a method, primer set, and kit for capturing the whole genome of Mycobacterium tuberculosis. The primer combination disclosed in this invention can effectively amplify the whole genome sequence of Mycobacterium tuberculosis with high coverage, which is beneficial for further research on the pathogenic mechanism, molecular epidemiology, and drug resistance mechanism of Mycobacterium tuberculosis.

The application discloses a primer group, a kit, a typing method and application for rapid typing of porcine delta coronavirus, and relates to the technical field of virus genome detection. The primer group comprises an upstream primer PDCoV-F, wherein the nucleotide sequence of the primer is shown as SEQ ID NO:1; and a downstream primer PDCoV-R, wherein the nucleotide sequence of the primer is shown as SEQ ID NO:2. The primer group is used for amplifying a core genome fragment obtained through screening, the fragment can represent the evolutionary topology structure of the whole genome, the typing resolution is higher than that of a traditional single gene method, and information distortion is avoided; the fragment is short in length, only needs conventional PCR and Sanger sequencing, the cost is reduced by more than 80%, data analysis is simple, and a professional biological information platform is not needed; an operation process is standardized, the flux is high, and the application is suitable for large-scale molecular epidemiological monitoring in a clinic; and the typing result can reflect the virus evolution history and the transmission path, and can provide a reliable and scientific basis for epidemic prevention and control.

The invention relates to an H3N2 subtype canine influenza virus strain and an H3N2 subtype canine influenza virus inactivated vaccine, the strain is named as A / canine / Shanghai / 0103 / 2019 (H3N2), and is preserved in the China General Microbiological Culture Collection Center (CGMCC). According to the research, the H3N2 subtype canine influenza virus A / canine / Shanghai / 0103 / 2019 (H3N2) is subjected to whole-genome sequence analysis, and molecular epidemiological data is provided for further prevention of canine influenza prevalence. Meanwhile, a template sequence is provided for developing an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) diagnostic kit of two surface gene segments HA and NA of the H3N2 subtype canine influenza virus, and a reserve of a seed strain is provided for developing a vaccine aiming at the H3N2 subtype canine influenza virus which is currently popular in China. The developed H3N2 subtype CIV inactivated vaccine can induce a dog to generate a relatively strong immune response reaction, in a challenge test, the severity of clinical symptoms of virus infection can be remarkably relieved, the detoxification period of viruses can be remarkably shortened, and the H3N2 subtype CIV inactivated vaccine has very good prevention and treatment effects on the currently popular H3N2 subtype CIV.

The application discloses a primer group, a kit, a typing method and application for rapid typing of porcine Senecavirus A, and relates to the technical field of virus genome detection. The primer group comprises an upstream primer SVA-F, wherein the nucleotide sequence of the upstream primer SVA-F is shown as SEQ ID NO:1; and a downstream primer SVA-R, wherein the nucleotide sequence of the downstream primer SVA-R is shown as SEQ ID NO:2. The primer group is used for amplifying a core genome fragment obtained through screening, the fragment can represent the evolutionary topology structure of the whole genome, the typing resolution is significantly higher than that of a traditional single gene method, and information distortion is avoided; the fragment is short in length, only needs conventional PCR and Sanger sequencing, the cost is reduced by more than 80%, data analysis is simple, and a professional biological information platform is not needed; an operation process is standardized, the flux is high, and the application is suitable for clinical large-scale molecular epidemiological monitoring; and a typing result can truly reflect the virus evolution history and the transmission path, and can provide a reliable and scientific basis for epidemic prevention and control.

The invention provides a general type avian infectious bronchitis virus whole genome deep sequencing method and application, and the method realizes efficient amplification of whole genome long fragments of IBV epidemic strains with different genotypes by designing and applying a set of combination containing 24 pairs of specific primers. The specific sequences of the primer combination are shown as SEQ NO: 1 to SEQ NO: 48. Compared with a traditional sequencing method, the method has the advantage that the sequencing cost is remarkably reduced. According to the method, the IBV full-length genome sequence exceeding 27 kb can be successfully obtained only through two PCR reactions, a more economical and efficient solution is provided for IBV related scientific research and molecular epidemiological monitoring, and scientific research and development in related fields are promoted.

The invention discloses an RPA (recombinase polymerase amplification) primer group for detecting bovine brucella, bovine babesia and pasteurella as well as a detection method and application thereof, the RPA primer group comprises three pairs of specific primers F1-R1, F2-R2 and F3-R3, whether a sample to be detected is bovine brucella, bovine babesia and pasteurella or not can be judged at one time, no false positive appears, and the RPA primer group can be used for detecting the bovine brucella, bovine babesia and pasteurella. Therefore, the method is simpler and more convenient, and the result is accurate; according to the method, the RPA technology is adopted, target DNA can be amplified at normal temperature within a short time, a theoretical basis and a scientific basis are provided for a molecular epidemic disease basis of bovine brucella, bovine babesia and pasteurella, sensitive, specific, simple and rapid bacterial detection is achieved, and the method has a profound application value in the fields of public health and the like.

The invention provides a specific M2-TAMs immunodiagnosis marker related to lung adenocarcinoma and application thereof. The technical key point is that SPAG4 is applied to preparation of a tumor diagnosis product as a diagnosis marker. In the early stage, a WGCNA algorithm is adopted, transcriptome data of lung adenocarcinoma patients in TCGA and GEO databases and M2 type macrophage distribution proportion data analyzed by CIBERSORT are integrated, and an M2 type macrophage polarization correlation module is successfully constructed. Through differential gene screening, Cox regression analysis and Kaplan-Meier survival analysis, the SPAG4 is finally determined as the core regulatory gene. Experimental results show that M2 type macrophage polarization and angiogenesis can be remarkably reduced by inhibiting expression of SPAG4, so that tumor growth is inhibited. On the basis, the advantages of an unmarked metabolism living body imaging technology, a living body blood vessel imaging technology, a living body immunostaining technology and molecular epidemiological research are integrated, the regulation and control mechanism of the SPAG4 gene in the lung adenocarcinoma progress and the influence of the SPAG4 gene on macrophage polarization and blood vessel generation are focused, and a novel tumor diagnosis technology and a kit are developed; the accuracy of early screening of the lung cancer is improved, and a new target spot is provided for precise treatment.