51 results about "Ubiquitinated Proteins" patented technology

The invention discloses a nanometer coordination polymer and a preparation method and application thereof. The nanometer coordination polymer comprises ditiocarb sodium, copper and a target ligand, the ditiocarb sodium and the copper form a polymer under the coordination effect, and the target ligand wraps the surface of the polymer under the electrostatic effect to form a core-shell structure. The preparation method of the nanometer coordination polymer comprises the steps that the ditiocarb sodium and a stabilizer are mixed to obtain a mixed solution, CuCl2 is dropwise added into the mixturethrough a constant flow pump, and a polymer is obtained; the target ligand is prepared into a solution, the solution is dropwise added into the polymer through the constant flow pump, and through stirring, the nanometer coordination polymer is obtained. According to the nanometer coordination polymer, poly-ubiquitinated protein accumulation can be caused, protein can be degraded and damaged, andthe p97 channel can be interfered, finally cell apoptosis is induced, and the nanometer coordination polymer can be applied to preparation of target apoptosis-promoting medicines for malignant tumor cells.

There is provided antibodies, epitopes and methods for detecting ubiquitinated polypeptides and ubiquitination sites in proteins. The antibodies recognize a fragment of ubiquitin that is created after samples are treated with the proteoloytical enzyme LysC (or LysN).

The invention relates to particular ubiquitination epitopes, antibodies that specifically recognize and bind to ubiquitinated proteins and peptides (particularly after the ubiquitin is removed by proteolytic cleavage) and to methods of using these epitopes and antibodies.

Methods for establishing a protein expression profile of a biological sample, by contacting a biological sample with a ubiquitin-binding protein such that ubiquitinated and specific non-ubiquitinated protein (the IPS) bind to the ubiquitin-binding protein; isolating the IPS proteins; and analyzing the isolated IPS proteins, wherein an expression profile is generated.