Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mutated PCV2 (Porcine circovirus Type 2) virus free from being degraded by ubiquitination proteasome, and preparation method and application thereof

A proteasome and ubiquitination technology, applied in biochemical equipment and methods, botanical equipment and methods, applications, etc., can solve problems such as unresearched

Active Publication Date: 2018-06-12
NORTHWEST A & F UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of pMKRN1 binding on the main structural protein component of PCV2, that is, its capsid protein (Cap), has not been studied.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mutated PCV2 (Porcine circovirus Type 2) virus free from being degraded by ubiquitination proteasome, and preparation method and application thereof
  • Mutated PCV2 (Porcine circovirus Type 2) virus free from being degraded by ubiquitination proteasome, and preparation method and application thereof
  • Mutated PCV2 (Porcine circovirus Type 2) virus free from being degraded by ubiquitination proteasome, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. What has been described is by way of explanation, not limitation, of the invention.

[0041] The present invention detects the effect of pMKRN1 on Cap degradation by co-expressing porcine MKRN1 (pMKRN1) and PCV2Cap to determine the effect of pMKRN1 on PCV2Cap degradation, then constructs a truncated mutant of PCV2Cap protein, and confirms the interaction region between pMKRN1 and PCV2Cap by co-immunoprecipitation assay , and further determined the interaction site between pMKRN1 and PCV2Cap by ubiquitination and degradation analysis. The PCV2 mutant constructed according to the site of action cannot be ubiquitinated, so that the replication ability of PCV2 in cells and in vivo is significantly improved. This discovery facilitates the study of the pathogenic mechanism of PCV2 and provides potential control measures for PCV2 outbreaks.

[0042] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a mutated PCV2 (Porcine circovirus Type 2) virus free from being degraded by ubiquitination proteasome, and a preparation method and application thereof. Three loci (164K, 179Kand 191K) of porcine circovirus 2 type (PCV2, GenBank and No. EU366323) strains Cap are mutated, the mutated PCV2 can not be subjected to MKRN1-mediated ubiquitination degrading, in addition, high speed copying can be carried out through infectious clone, and virus price is high than virus price of wild-type PCV2. Through mutated viruses obtained by the infectious clone, the pathogenic mechanism of the PCV2 can be more favorably researched, and a potential control measure is provided for PCV2 outbreak.

Description

technical field [0001] The invention relates to pMKRN1-mediated porcine circovirus type 2 (PCV2) strain Cap ubiquitination, in particular to the construction of PCV2 with amino acid site mutations related to pMKRN1 ubiquitin ligase action. Background technique [0002] Porcine circovirus associated disease (PCVAD) is caused by porcine circovirus type 2 (PCV2), mainly including porcine dermatitis and nephropathy syndrome (PDNS) , piglet congenital tremors (congenital tremors, CT) and postweaning multisystemic wasting syndrome (postweaning multisystemic wasting syndrome, PMWS) and other diseases. Although PCV2 can replicate in PK-15 cells, it has not been able to reproduce at a high speed, which hinders the research on the pathogenic mechanism of PCV2. How to improve the replication ability of PCV2 is one of the urgent problems to be solved in the study of its pathogenic mechanism. [0003] Studies have shown that human MKRN1 functions as an E3 ubiquitin ligase on a variety ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00C12N15/34C12N5/071
CPCC12N5/0686C12N7/00C12N2750/10021
Inventor 黄勇童德文王彤彤
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com