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Application of E3 ubiquitination protein ligase UBR5 in preparation of tumor diagnosis or prognosis evaluation kit

A technology of ubiquitinated protein and UBR5, which is applied in instruments, measuring devices, scientific instruments, etc., and can solve the problems of unproven clinical significance of expression

Pending Publication Date: 2020-11-10
SHANGHAI CITY PUDONG NEW AREA GONGLI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the expression of UBR5 in ccRCC and its clinical significance have not been confirmed

Method used

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  • Application of E3 ubiquitination protein ligase UBR5 in preparation of tumor diagnosis or prognosis evaluation kit
  • Application of E3 ubiquitination protein ligase UBR5 in preparation of tumor diagnosis or prognosis evaluation kit
  • Application of E3 ubiquitination protein ligase UBR5 in preparation of tumor diagnosis or prognosis evaluation kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Paraffin sections of clear cell renal cell carcinoma tissues from 310 patients with clear cell renal cell carcinoma were selected (310 clear cell renal cell carcinoma tissue sections were obtained from Shanghai Changhai Hospital, and were diagnosed as clear cell renal cell carcinoma by 2 pathologists), and immunohistochemical methods were used to detect The expression level of E3 ubiquitinated protein ligase UBR5 in tumor tissue and adjacent normal tissue of patients with clear cell renal cell carcinoma and the calculation of immunohistochemical score, the specific steps are as follows:

[0067] (1) Paraffin sections of clear cell renal cell carcinoma tissue were prepared, and the oven was set at 60°C overnight;

[0068] (2) slices are dewaxed to water;

[0069] (XyleneⅠ①10min→XyleneⅡ②10min→XyleneⅢ③10min→100% ethanol 5min→95% ethanol 5min→85% ethanol 5min→75% ethanol 5min→double distilled water 5min)

[0070] (3) 3%H 2 o 2 Solution, placed at room temperature for 20m...

Embodiment 2

[0089] Combined with the prognosis information, the 310 patients with clear cell renal cell carcinoma in Example 1 were randomly divided into a training group and a verification group at a ratio of 3:2. Using the H-score score of the E3 ubiquitinated protein ligase UBR5 in the 186 patients in the training group, the receiver operating characteristic curve (Receiver Operating Characteristic Curve, ROC) analysis was performed according to the 5-year survival time, and the data analysis was performed using R software ( 3.5.3 version), the best cut-off value (cut-off value) for UBR5 to predict the prognosis of ccRCC patients is 130 see figure 2 ).

Embodiment 3

[0091] Combined with the prognosis information, 186 patients in the training group and 124 patients in the verification group were divided into a UBR5 high expression group (H-score greater than or equal to 130) and a UBR5 low expression group (H-score less than or equal to 130) according to the cut off value obtained in Example 2. 130), for statistical analysis of pathological features, data analysis using SPSS software (version 25.0), the results showed that the UBR5 high expression group had lower TNM stage, WHO / ISUP nuclear grade and SSIGN score (see Table 1-2).

[0092] Table 1 Correlation between UBR5 expression and clinicopathological features in patients with clear cell renal cell carcinoma in the training group

[0093]

[0094] Table 2 Correlation between UBR5 expression and clinicopathological features in patients with clear cell renal cell carcinoma in the verification group

[0095]

[0096]

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Abstract

The invention relates to the field of biotechnology and medical diagnosis, in particular to application of E3 ubiquitination protein ligase UBR5 in preparation of a renal clear cell carcinoma diagnosis or prognosis evaluation reagent or kit and a corresponding detection kit. Researches find that the expression of E3 ubiquitination protein ligase UBR5 molecules in patients with renal clear cell carcinoma is generally reduced, the expression level of the E3 ubiquitination protein ligase UBR5 molecules is remarkably related to prognosis of the patients, and the total lifetime (OS) and progression-free lifetime (PFS) of the patients with renal clear cell carcinoma can be better predicted by combining the expression of UBR5.

Description

technical field [0001] The invention relates to the fields of biotechnology and medical diagnosis, in particular, the application of E3 ubiquitinated protein ligase UBR5 in the preparation of a diagnostic or prognosis evaluation kit for renal clear cell carcinoma. Background technique [0002] Kidney cancer is one of the most common cancers in both men and women worldwide. (Barata PC, Rini BI. Treatment of renal cell carcinoma: Current status and future directions. CA Cancer JClin. 2017; 67(6): 507-524.) Clear Cell Renal Cell Carcinoma (ccRCC) is the most common types of renal cell carcinoma (Shuch B, Amin A, Armstrong AJ, et al. Understanding pathologic variants of renal cell carcinoma: distilling therapeutic opportunities from biological complexity. Eur Urol. 2015; 67(1): 85-97). Patients with localized ccRCC are generally treated with surgery, and with the progress in recent years, the side effects have been significantly reduced (Hsieh JJ, Purdue MP, Signoretti S, et al...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/574G01N33/573
CPCG01N33/577G01N33/57484G01N33/57438G01N33/573
Inventor 崔心刚王超洪天宇王宇宁田毅君彭广王鸿儒姜宁李健储传敏章璟
Owner SHANGHAI CITY PUDONG NEW AREA GONGLI HOSPITAL
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