Nanometer coordination polymer and preparation method and application thereof

A technology of coordination polymers and polymers, which is applied in pharmaceutical formulations, medical preparations without active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of high cost, toxic and side effects, and reduce toxic and side effects. , The effect of low package load and high process drug load

Active Publication Date: 2019-11-05
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these drugs can cause serious side effects and are expensive

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  • Nanometer coordination polymer and preparation method and application thereof
  • Nanometer coordination polymer and preparation method and application thereof
  • Nanometer coordination polymer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A nano-coordination polymer, including sodium disulfide and copper to form a polymer through coordination.

[0059] The nano-coordination polymer of this embodiment is prepared by the following method:

[0060] (1) Take 6 mL of 2 mM dithiocarbamate (DTC) into a 20 mL beaker, add 184 μL of 20 wt % polyvinylpyrrolidone (PVP), and stir at room temperature for 2 min to obtain a mixed solution.

[0061] (2) Use a 5mL syringe to draw 3mL of 2mM CuCl 2 Use a constant flow pump to drip into the mixed solution of the above step (1) with a drop rate of 20 μL / min to obtain sodium dithiocarbamate / copper nano coordination polymer (DTC-Cu 2+ NPs). The reaction solution was dilute to 10 mL for later use.

Embodiment 2

[0063] A nano-coordination polymer, including sodium dithiocarbamate, copper and hyaluronic acid, sodium dithiocarbamate and copper form a polymer through coordination, and hyaluronic acid wraps the above polymer through electrostatic interaction to form a core-shell structure.

[0064] The nano-coordination polymer of this embodiment is prepared by the following method:

[0065] (1) Take 6 mL of 2 mM dithiocarbamate (DTC) into a 20 mL beaker, add 184 μL of 20 wt % polyvinylpyrrolidone (PVP), and stir at room temperature for 2 min to obtain a mixed solution.

[0066] (2) Use a 5mL syringe to draw 3mL of 2mM CuCl 2 Use a constant flow pump to drip into the mixed solution of the above step (1) with a drop rate of 20 μL / min to obtain sodium dithiocarbamate / copper nano coordination polymer (DTC-Cu 2+ NPs). The reaction solution was dilute to 10 mL for later use.

[0067] (3) Accurately weigh 12 mg of hyaluronic acid (HA), dissolve it in 20 mL of distilled water, and prepare a ...

experiment example

[0070] 1. Particle size: detect DTC-Cu separately 2+ NPs and DTC-Cu 2+ The particle size of @HA NPs is measured as follows: take the sample solution and place it in a Marlven Nano ZS instrument, use the dynamic light laser scattering method to detect the particle size, set the temperature of the measurement cell to 25°C, and operate 3 copies of each sample in parallel. figure 1 for DTC-Cu2 + NPs, DTC-Cu 2+ The particle size and potential change diagram of @HA NPs, from the results of the diagram, it can be known that the nano-coordination polymer DTC-Cu 2+ The particle size of NPs is 98nm, the potential is +1.73mV, and the coordination polymer DTC-Cu coated with hyaluronic acid 2+ The particle size of @HA NPs increased to 125nm, and the potential decreased to -23.2mV.

[0071] 2. Morphology: observe DTC-Cu 2+ The morphology of @HA NP, the detection method of the morphology: the sample is dropped on a 400-mesh copper grid covered with a carbon film, placed in a desiccator,...

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Abstract

The invention discloses a nanometer coordination polymer and a preparation method and application thereof. The nanometer coordination polymer comprises ditiocarb sodium, copper and a target ligand, the ditiocarb sodium and the copper form a polymer under the coordination effect, and the target ligand wraps the surface of the polymer under the electrostatic effect to form a core-shell structure. The preparation method of the nanometer coordination polymer comprises the steps that the ditiocarb sodium and a stabilizer are mixed to obtain a mixed solution, CuCl2 is dropwise added into the mixturethrough a constant flow pump, and a polymer is obtained; the target ligand is prepared into a solution, the solution is dropwise added into the polymer through the constant flow pump, and through stirring, the nanometer coordination polymer is obtained. According to the nanometer coordination polymer, poly-ubiquitinated protein accumulation can be caused, protein can be degraded and damaged, andthe p97 channel can be interfered, finally cell apoptosis is induced, and the nanometer coordination polymer can be applied to preparation of target apoptosis-promoting medicines for malignant tumor cells.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a nano-coordination polymer and its preparation method and application. Background technique [0002] Malignant tumor is a major disease that seriously threatens the safety of human life, with high morbidity and mortality. Currently, there is no effective treatment. Most tumor tissues have the characteristics of malignant proliferation. Compared with normal tissues, the structure of neovascularization in tumor tissues is less complete, with weak vessel walls, lack of smooth muscle and complete basement membrane structure. There are large gaps between endothelial cells and strong permeability, which is conducive to the retention of nanomedicine in tumor sites. At the same time, due to the activation and expression of tumor-related genes, some specific receptors are highly expressed on the surface of tumor cells, such as the triple-negative breast cancer cell MDA-MB-231 highly ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00A61K47/69A61K47/61A61K47/62A61K47/52A61P35/00
CPCA61K47/52A61K47/61A61K47/62A61K47/6925A61P35/00C08G83/008A61K9/513A61K33/34A61K47/10A61K47/32C08G83/001A61K47/02A61K47/20
Inventor 周文虎彭颖曹丽霞丁劲松
Owner CENT SOUTH UNIV
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