161 results about "Skin test" patented technology

There is provided an inexpensive microneedle device for diagnosis of an allergy, enabling one to perform a clear skin test with simple operation for a short period of time in the diagnosis of allergies. The microneedle device (1) comprises: a microneedle substrate (2), microneedles (3) formed on the substrate at a density of 100 to 10000 needles per 1 cm², being capable of piercing the skin 50 μm to 500 μm deep, and allergen-holding means (4) formed on the microneedles, holding at least one allergen. The microneedles (3) can be prepared by the use of a non-metallic synthetic or natural resin material.

The present invention is based on the identification and characterization of a number of novel M. tuberculosis derived proteins and protein fragments. The invention is directed to the polypeptides and immunologically active fragments thereof, the genes encoding them, immunological compositions such as vaccines and skin test reagents containing the polypeptides.

The present invention is based on the identification and characterization of a number of M. tuberculosis derived novel proteins and protein fragments (SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 17-23, 42, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72-86, 88, 90, 92, 94, 141, 143, 145, 147, 149, 151, 153, and 168-171). The invention is directed to the polypeptides and immunologically active fragments thereof, the genes encoding them, immunological compositions such as vaccines and skin test reagents containing the polypeptides. Another part of the invention is based on the surprising discovery that fusions between ESAT-6 and MPT59 are superior immunogens compared to each of the unfused proteins, respectively.

The invention relates to an extension testing device in the technical fields of material mechanics experiments and building structure material tests. The extension testing device provided by the invention comprises a frame component, four groups of extension mechanisms and a specimen fixing component, wherein the frame component is respectively and fixedly connected with the four groups of extension mechanisms and the specimen fixing component. The extension testing device provided by the invention fully utilizes the characteristics of a cylinder, a clamping component and a linear guide rail, has the advantages of multiple test piece dimensions, multiple test functions and large test tension range and can be used for building film material tests and airship skin tests.

The invention provides a preparation method of a Traditional Chinese medicament for treating swelling tympanitis in an external auditory meatus, and belongs to the technical field of a preparation method of a Traditional Chinese medicament. Sulfonamides and antibiotics are usually used for treating swelling tympanitis in the external auditory meatus; and if using penicillin, a skin test needs to be performed before use, and a positive person is not allowed to use. The Traditional Chinese medicament is prepared from the following bulk drugs: Chinese mahonia leaf, tender catchweed bedstraw herb, dysosma versipellis, Indian stringbush root, herb saururus chinensis, garden erphorbia herb, longhairy antenoron herb, omoto nipponlily root and rhizome, climbing groundsel herb, all-grass of snakefoot clubmoss, Japanese stephania root, thymifoious euphorbia herb, Chinese brake herb, fruit of tree-of-heaven ailanthus, magnolia flower, dahurian angelica, ginger, cocklebur fruit, chastetree fruit, ligusticum sinense, peppermint, lindley butterflybush herb, cicada slough, scabrous patrinia root, centipeda minima, perilla leaf, fistular onion stalk, notoperygium root and liquorice. The preparation method comprises the following steps: soaking the 29 bulk drugs in water; decocting with slow fire; and filtering to remove residues to obtain decoction, namely the Traditional Chinese medicament for treating swelling tympanitis in the external auditory meatus. The Traditional Chinese medicament has the advantages of simple preparation process, short course of treatment, high cure rate and capability of avoiding adverse reaction caused by western medicaments.

The present invention is based on the identification and characterization of a number of novel M. tuberculosis derived proteins and protein fragments. The invention is directed to the polypeptides and immunologically active fragments thereof, the genes encoding them, immunological compositions such as vaccines and skin test reagents containing the polypeptide.

The invention discloses a composition with skin-whitening and anti-ageing effects, a skin care product containing the composition as well as a preparation method and an application of the composition. The composition is prepared from the following components: 0.2%-10.0% of L-ascorbic acid 2-glucoside, 0.1%-0.2% of superoxide dismutase, 0.1%-0.5% of a grape seed extract, 0.1%-0.5% of a soybean fermentation extract, 10%-30% of propanediol, 0.1%-0.2% of aloe powder and the balance amount of water. An experimental result shows that the composition has the skin-whitening and anti-ageing effects, the method is easy in operation, the production cost is low, and the product stability is high; an evaluation on an in vitro test and a human body skin test proves that the composition has good skin-whitening and anti-ageing effects and is safe and free from side effects; meanwhile, the composition and the skin care product containing the composition, provided by the invention, also have better long-lasting hydrating effects.

The invention provides a skin evaluation method and a skin care product or cosmetic recommendation method by the use of a laser speckle blood perfusion imaging instrument. The skin evaluation method comprises a shooting step, an imaging step, an area selection step and an evaluation step. In the shooting step, a video with specified time and showing surface blood signals of a cleaned face is shot. In the imaging step, all pixels, representing the blood signals, of the video are averaged so as to obtain face blood perfusion images. In the area selection step, blood vessel distribution parts different from other parts of the face are removed, and the residual parts are selected as evaluation zones. In the evaluation step, evaluations on blood perfusion amounts are carried out, and therefore the skin is evaluated. By employing the skin evaluation method, multiple different skin test indexes can be evaluated through a single apparatus, and therefore a test process is simplified and accelerated.

Test methods for assessing irritation of portions of skin and/or assessing inhibition and/or reduction of such irritation are provided.

The invention provides a method for preparing an allergen diagnostic reagent of acute allergic diseases caused by dermatophagoides pteronyssinus (Der p for short). The allergen diagnostic reagent is Der p allergen pricking liquid. The invention also provides a method for preparing an allergen diagnostic reagent raw material, namely Der p pure mite allergen, a method for evaluating the valence of the allergen diagnostic reagent and a purpose for specifically diagnosing the allergic diseases. The allergen diagnostic reagent achieves the aim for detecting whether a patient is allergic to a matter to cause a disease by a sensitized principle. The Der p pure mite allergen raw material has short culture period and high purity, and the pricking liquid made of the raw material has high activity, thereby greatly reducing a skin test risk of the allergic patient and improving the safety of clinical medical treatment.

The invention provides an electro-hydraulic loading system of a thin-film biaxial tensile testing machine, belonging to the technical field of mechanics of materials and building materials. The system provided by the invention comprises a low-pressure filter, a hydraulic pump, a motor, a check valve, a pressure sensor, a stop valve, a power accumulator, an electromagnetic unloading valve, a proportional overflow valve, a throttling valve, an electromagnetic directional valve, four servo hydraulic cylinders, a drawing force and compression force sensor, a displacement sensor, a mechanical clamp, a filter, a cooler, an electromagnetic water valve, an oil tank and a unidirectional intercept valve. The system provided by the invention has the advantages of great drawing force, high precision,fast control response, simplicity, special purpose, movability and independent use and can be used for the thin-film composite material biaxial tensile testing machines of construction film material tests, airship skin tests and the like.

The invention relates to the technical field of cosmetology and personal health care, in particular to a skin health detector. The skin health detector detects and analyzes skin, a detector body for detecting moisture and oil content of the skin is connected with a control unit, an LED digital display unit connected with the control unit displays test numerical values, and data is synchronized to an external device through a Bluetooth module. The detector further comprises a water tank. An atomization sheet is contained in the water tank and connected with the control unit so as to moisturize the skin. The intelligent skin health detector manages skin test data and gives corresponding skin care suggestions through the external device. The intelligent skin health detector is simple in structure and convenient to operate, skin detection and atomization can be intelligently conducted, and volume production can be achieved.

Thioredoxin, a small dithiol protein, is a specific reductant for major food proteins, allergenic proteins and particularly allergenic proteins present in widely used foods from animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food. Studies showed increased digestion of food and food proteins by pepsin and trypsin following reduction by thioredoxin.

The invention discloses an electro-hydraulic loading system of a film biaxial tensile testing machine. The electro-hydraulic loading system comprises a low-pressure filter, hydraulic pumps, a motor, a one-way valve, a stop valve, an energy storage device, an electromagnetic unloading valve, a proportional overflow valve, a pressure compensator, a proportional directional valve, a servo hydraulic cylinder, a pull pressure sensor, a displacement sensor, a mechanical fixture, a filter, a cooler and an oil tank, wherein the two hydraulic pumps share the one oil tank, and two independent loading hydraulic loops can be formed, so that the manufacturing cost and the installation space are greatly saved. Furthermore, the servo hydraulic cylinder can be controlled by the single set of pressure compensator and proportional directional valve, so that loading synchronism and stability can be guaranteed. The electro-hydraulic loading system of the film biaxial tensile testing machine has the characteristics of being simple in structure, large in tensile force, high in loading synchronism and accuracy, rapid in control response and the like, and can be used for film composite material biaxial tensile testing machines such as an architectural membrane material tester and an airship skin test as well as a uniaxial drawing tester.

The invention provides a recombinant protein of a mycobacterium tuberculosis specific marker Rv 3120. The recombinant protein comprises an amino acid sequence as shown in SEQ ID NO.1. The invention also provides a coding nucleotide sequence of the recombinant protein of the mycobacterium tuberculosis Rv 3120, a preparation method of the recombinant protein, and an application of the recombinant protein. According to the invention, a mycobacterium tuberculosis immunodominant antigen Rv 3120 is first reported on the basis of the research results of immunomics. The mycobacterium tuberculosis immunodominant antigen Rv 3120 is applied to the cellular immunological diagnosis of tuberculosis, and has relatively high sensibility; and moreover, compared with conventional skin test, the mycobacterium tuberculosis immunodominant antigen Rv 3120 has the advantages of faster performance, safety and reliability.

The invention relates to a method, device, mobile phone, computer device and storage medium for evaluating skin, and belongs to the technical field of evaluation. The method comprises the following steps of: sending an illumination instruction to an illumination unit; wherein the illumination instruction is used for triggering the illumination unit to emit specific skin test light; the skin test light is used to irradiate the skin of a subject; acquiring a skin image acquired by a shooting device; wherein the skin image includes an image of the skin of the subject when irradiated by the skin test light; evaluating a skin state of the subject based on the skin image. According to the technical scheme, the problem that skin evaluation is not convenient enough is solved. The illumination unitis controlled to emit the specific skin test light, and the skin state of the subject can be conveniently analyzed according to the skin image acquired under the specific light source.

The invention belongs to the field of biological engineering and diagnostic medicine, in particular to a mycobacterium tuberculosis ESAT-6 recombinant dipolymer, a preparation method thereof, an antigenicity analysis and an application thereof in the serodiagnosis of tuberculosis. The invention uses the DNA of a chimeric gene nucleic acid vaccine HG856A plasmid containing 2 * esat-6 copies as the template, obtains 2 * esat-6 gene segments by PCR amplification, and clones, expresses and purifies an ESAT-6 recombinant dipolymer protein in E.coli. The protein is connected with the two ESAT-6 monomers through amino acid Tyr and Val which are coded by the AccI restriction enzyme cutting site, i.e. GTC TAC, carries six* His tags at the N end, and can be purified by nickel-chelate affinity chromatography. Experiments confirm that the purified recombinant dipolymer has favorable reactogenicity and antigenic specificity. The recombinant dipolymer can be used as an antigen for the early diagnosis of the tuberculosis, including the serodiagnosis of ELISA, ELISPOT or the like, or can be used as an antigen for a skin test.

The invention relates to a skin test solution preparation device and a method for preparing a skin test solution. The skin test solution preparation device comprises an injector cylinder and an injector piston inserted in the injector cylinder. The injector cylinder is composed of a main cylinder body and a micro measurement cylinder body which is connected to the front end of the main cylinder body, and the front end of the micro measurement cylinder body is connected with a syringe needle. The injector piston is composed of a main piston body and an auxiliary piston body. An auxiliary piston cavity is formed in the main piston body, and the auxiliary piston body is inserted in the auxiliary piston cavity. The rear end of the main piston body is connected with a main piston rod, the rear end of the auxiliary piston body is connected with an auxiliary piston rod, an inner cavity is formed in the main piston rod in the axial direction, the auxiliary piston rod penetrates through the inner cavity, and the auxiliary piston body is matched with the micro measurement cylinder body. According to the skin test solution preparation device and the method for preparing the skin test solution, steps of skin test solution preparation are simplified, the probability of solution pollution is reduced, operation frequency is reduced, preparation precision is improved, and errors cannot occur easily; measurement is accurate, there is no residue or bubble or bacterium when the solution is emptied by the preparation device, and operation is convenient and quick.

Fibrous structures that exhibit improved surface properties, for example lower Force Variability Values as measured by the Glide on Skin Test Method described herein, compared to known fibrous structures, sanitary tissue products comprising such fibrous structures and method for making such fibrous structures are provided.

The invention discloses a production process of a high-titer tuberculin skin test diagnostic reagent PPD (Purified Protein Derivative). The production process comprises bacterial culture, inactivationand precipitation. The bacterial culture specifically comprises the following process steps: culturing and developing a working seed culture into dry agglomerated light-yellow bacterial lawn, performing microscopic examination on the bacterial lawn as bacillus brevis that is micro bent and round in two ends; and performing two-generation culture to obtain wrinkled light yellow mycoderm, performing passage culture for three generations when the mycoderm overspreads the surface of the culture medium and the culture solution is clarified and transparent, so as to form multi-wrinkled light yellowmycoderm, and performing four-generation culture for 8-10 weeks. According to the production process of the high-titer PPD capable of enriching two antigens such as ESAT-6 and CFP10, the PPD preparedby the process disclosed by the invention has high titer, and the content of the ESAT-6 and CFP10 is high.

The invention discloses a multifunctional thin film three-axis tensile testing machine. The multifunctional thin film three-axis tensile testing machine comprises a horizontal double-axis testing device and a Z-axis tensile testing device, wherein the Z-axis tensile testing device is constructed above the horizontal double-axis testing device; the Z-axis tensile testing device comprises second stand columns, frameworks, a first fixed guide rail beam, a second fixed guide rail beam, a movable guide rail beam, a Z-axis platform, a first fixed plate, a movable rod, an electric cylinder, a second tension sensor and a second clamp. The invention further discloses a thin film three-axis tensile testing method which comprises a plurality of steps. The multifunctional thin film three-axis tensile testing machine and the testing method can realize multi-angle tension and have a multi-test function; the multi-angle tension comprises unidirectional static tension, low-cycle circulation back-and-forth tension, bidirectional static tension, low-cycle circulation back-and-forth tension with any double-axis stress ratio, three-dimensional connection part tension and low-cycle circulation back-and-forth tension with any three-axis stress ratio; the multifunctional thin film three-axis tensile testing machine is stable in operation, and the multifunctional thin film three-axis tensile testing machine and the testing method can be used for building membrane tests, airship skin tests and the like.