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51results about How to "Method saves time" patented technology

Method for preparing high molecular weight biodegradable polyester by combined chain extension

The invention relates to a method for preparing biodegradable polyester. Firstly, melt polycondensation is conducted through diol and dicarboxylic acid and a low molecular weight polyester prepolymer with number average molecular weight between 2, 000 and 20, 000 or intrinsic viscosity below 0.64dL / g is prepared; binary oxadiazoline and diacyl bis-caprolactamate are combined to extend chain, and chain extension is carried out at a certain temperature; and the biodegradable polyester is obtained. The number average molecular weight of polybutylene adipate (PBA) can reach up to 39, 000 and the intrinsic viscosity to 1.13dL / g. For poly (butylene succinate) (PBS) with relatively high melting point, the intrinsic viscosity after chain extension is up to 0.99dL / g.
Owner:BEIJING UNIV OF CHEM TECH

Method for preparing carbon nitride/ titanium dioxide heterojunction photocatalyst in one-step electrostatic spinning way

The invention belongs to the technical field of photocatalysts for degrading organic sewage, and specifically relates to a method for preparing a carbon nitride / titanium dioxide heterojunction photocatalyst in one-step electrostatic spinning way. The heterojunction photocatalyst is prepared in the one-step electrostatic spinning way, wherein the one-step electrostatic spinning way specifically comprises the following steps: adding melamine or guanidine hydrochloride or urea to a mixed solution containing glacial acetic acid, tetrabutyl titanate, polyvinylpyrrolidone and an organic solvent; then performing the electrostatic spinning technology and the high-temperature sintering treatment to obtain the carbon nitride / titanium dioxide heterojunction photocatalyst. The prepared photocatalyst is outstanding in heterojunction and outstanding in performance of photocatalyzing degrading the organic sewage; the preparation technology of the carbon nitride / titanium dioxide heterojunction photocatalyst is simplified; the technology is simple; the time is saved. Therefore, the method has a good application prospect in organic sewage treatment.
Owner:WUHAN POLYTECHNIC UNIVERSITY

Method for preparing nonspherical, raspberry-shaped or hollow polymer microspheres through one-pot dispersion polymerization

The invention provides a method for preparing nonspherical, raspberry-shaped or hollow polymer microspheres through one-pot dispersion polymerization, and belongs to the technical field of dispersion polymerization. The method comprises the steps of stirring monomers styrene St and sodium styrenesulfonate NaSS and an initiator azodiisobutyronitrile AIBN, and dissolving the mixture in the mixed solution of an organic solvent and water to form a reaction solution; increasing the temperature of the reaction solution to the range from 75 to 80 DEG C and reacting for a certain of time; next, dropwise adding a cross-linking agent or the cross-linking and other reaction reagents to the reaction system, and further reacting for a while to obtain nonspherical, raspberry-shaped or hollow polymer microspheres. According to the invention, a method for directly preparing different types (nonspherical, raspberry-shaped or hollow) of polymer microspheres based on conventional dispersion polymerization and by controlling the cross-linking reaction process and adjusting reaction parameters is established.
Owner:BEIJING UNIV OF CHEM TECH

Preparation method for biodegradable aliphatic alternating polyester amide

The invention discloses a preparation method for biodegradable aliphatic alternating polyester amide. According to the preparation method, aliphatic dicarboxylic acid and diamide diol are subjected to melt phase polycondensation to prepare alternating polyester amide prepolymer with terminal carboxyl group and terminal hydroxy group; and diacyl di-lactam and a bisoxazoline chain extender are subjected to joint chain extension to prepare the biodegradable aliphatic alternating polyester amide with the intrinsic viscosity of 0.30-0.59dL / g. The preparation method disclosed by the invention has the characteristics of simple and convenient method, mild conditions, high efficiency and the like and can be used for preparing the biodegradable aliphatic alternating polyester amide with regular structure and favorable crystallinity.
Owner:BEIJING UNIV OF CHEM TECH

Graphene-basalt fiber composite material and preparation method thereof

The invention discloses a graphene-basalt fiber composite material and a preparation method thereof. The cross-linking of the basalt fiber can be achieved by utilizing the swelling and fusion effectsof graphene oxide coating on the surface of the basalt fiber. The graphene oxide is taken as a sizing agent and a cross-linking agent, the basalt fiber can be evenly coated with the grapheme oxide, the cross-linking process is fast and easy, and the cross-linking intensity is high. After further deoxidization, a grapheme oxide cross-linking layer converts into a graphene cross-linking layer, the interaction between the basalt fibers is enhanced, the contact resistance between fibers is decreased, so that the basalt fiber demonstrates excellent mechanical property and electric and thermal conductivity, which can be used for reinforcement phase of the composite material and differentiated fiber application. The method of cross-linking the basalt fiber with the graphene is of great research value and extensive application prospect.
Owner:HANGZHOU GAOXI TECH CO LTD

Low temperature preparing process for anatase phase nano crystal titanium dioxide of light catalystic activity

InactiveCN1491897AUniform sizeStrong surface adsorption capacityTitanium dioxideChemistryTitanium dioxide
The present invention relates to liquid phase process of preparing anatase phase nano crystal titanium dioxide at the temperature range of room temperature to 120 deg.c. The process includes the alkali alkaline hydrolysis of hydrated titanium dioxide as main material, cooling, adding crystal kernel promoter, water washing, acid dissolving and cooling to obtain titanium dioxide slurry with photocatalytic activity; and further washing, filtering, drying and crushing to obtain anatase phase nano crystal titanium dioxide with photocatalytic activity. The nano crystal titanium dioxide has average granularity of 10-80 nm, specific surface area of 150-300 sq m / g, powerful surface adsorption capacity, very high photocatalytic activity and organic matter degrading capacity much higher than available similar marketable products.
Owner:INST OF CHEM CHINESE ACAD OF SCI

Multiple reverse transcription polymerase chain reaction detection method for swine transmissible gastroenteritis

The invention relates to a multiple reverse transcription polymerase chain reaction detection method for swine transmissible gastroenteritis. An antibody is needed to be prepared by an euzymelinked immunosorbent assay, so that the period is longer, and external factors have larger interference on the diagnostic method of the euzymelinked immunosorbent assay. The method comprises the following steps of: designing and synthesizing a transmissible swine gastroenteritis virus gene primer; establishing a multiple reverse transcription polymerase chain reaction diagnostic method of the transmissible swine gastroenteritis viruses and porcine epidemic diarrhea viruses; optimizing concentration of the primer of a bigeminy reverse transcription polymerase chain reaction; optimizing the concentration of magnesium oxide of the bigeminy reverse transcription polymerase chain reaction; optimizing the concentration of triphosphoric acid base deoxynucleotide of the bigeminy reverse transcription polymerase chain reaction; determining the optimal annealing temperature of the bigeminy reverse transcription polymerase chain reaction; testing the sensitivity of the bigeminy reverse transcription polymerase chain reaction; and testing the specificity of the bigeminy reverse transcription polymerase chain reaction. The multiple reverse transcription polymerase chain reaction detection method for swine transmissible gastroenteritis is suitable for detecting swine transmissible gastroenteritis.
Owner:郑世民

Graphene-glass fiber composite material and preparation method thereof

InactiveCN107761249AEvenly distributedHigh interface adhesion strengthGlass making apparatusNon-woven fabricsFiberGlass fiber
The invention discloses a graphene-glass fiber composite material and a preparation method thereof. Crosslinking of glass fibers can be realized by means of the swelling and fusing actions of grapheneoxide coatings on the surfaces of the glass fibers. Graphene oxide is taken as a sizing agent as well as a crosslinking agent with which the glass fibers can be coated uniformly, the crosslinking process is rapid and easy, and high crosslinking strength is achieved. Through further reduction, a graphene oxide crosslinking layer is turned into a graphene crosslinking layer, so that mutual action among the glass fibers is enhanced, contact resistance among the fibers is lowered, and the glass fibers show excellent mechanical performance, electric conductivity and heat conductivity and can be applied to a wild phase of the composite material. The method for crosslinking the glass fibers with the graphene has a great researching value and a wide application prospect.
Owner:HANGZHOU GAOXI TECH CO LTD

Real-time fluorescent PCR (polymerase chain reaction) detection primer probe set, kit and method for African swine fever virus CD2V gene

The invention belongs to the technical field of molecular biology, and discloses a real-time fluorescent PCR (polymerase chain reaction) detection primer probe set, kit and method for African swine fever virus CD2V gene A nucleotide sequence of a forward primer of the primer probe set is shown as SEQ ID NO. 4, a nucleotide sequence of a reverse primer is shown as SEQ ID NO. 5, and a nucleotide sequence of a specific fluorescent probe is shown as SEQ ID NO. 6, wherein a fluorescent reporter group is marked at the 5' end of the specific fluorescent probe, while a fluorescent quenching group is marked at the 3' end of the specific fluorescent probe. The primer probe set and kit provided by the invention help quickly, conveniently, efficiently and specifically detect whether a sample containsAfrican swine fever virus and whether the sample is identified as CD2V gene deletion, and are suitable for clinical identification detection.
Owner:南宁壮博生物科技有限公司

Biodegradable alternate poly tetraamide diester preparation method

InactiveCN103881085AImprove thermal performanceRealize hybrid chain extensionPolymer scienceCrystallinity
The invention relates to a biodegradable alternate poly tetraamide diester preparation method, and belongs to the technical field of polyamide esters. The preparation method comprises that: an aliphatic diamine and a dicarboxylic acid ester are subjected to a reaction to prepare a diamide diester, the diamide diester reacts with alcohol amine to obtain tetraamide diol, the tetraamide diol and a dicarboxylic acid are subjected to condensation polymerization to prepare an alternate poly tetraamide diester prepolymer, and chain extenders such as bisoxazoline and diacyl di-lactam are adopted to carry out chain extension on the prepolymer to prepare the biodegradable alternate poly tetraamide diester with the 30 DEG C intrinsic viscosity of 0.26-0.45 dL / g. The biodegradable alternate poly tetraamide diester has characteristics of regular structure, good crystallinity, high melting point, good thermal property, good mechanical property, and green and pollution-free synthesis route.
Owner:BEIJING UNIV OF CHEM TECH

High frequency circuit radiation electromagnetic inference analysis method

The invention discloses a high frequency circuit radiation electromagnetic inference analysis method. Two near field electric field probes or near field magnetic field probes of the same type are respectively connected with the two input ends of a digital dual-trace oscilloscope. The two near field probes are arranged in different positions to measure a high frequency circuit. Blind signal analysis is carried out on time domain signals obtained by the oscilloscope. A single radiation source in the high frequency circuit can be differentiated according to the signal analysis result. The near field electric field probes and the near field magnetic field probes are respectively connected with the input ends of a spectrum analyzer to measure the near field electric field and the near field magnetic field of the single radiation source and compare the size of the near field electric field and the near field magnetic field. In this way, whether the electromagnetic inference of each radiation source is caused by common mode radiation or difference mode can be known. The method saves time, is convenient and practical and can provide guidance for high frequency circuit radiation EMI suppression.
Owner:SOUTHEAST UNIV

Acetonitrile purification method

The invention discloses an acetonitrile purification method. The purification method comprises the following steps: (1) adding a strong alkali solid into crude acetonitrile to be treated to perform pretreatment; (2) passing through a strong-acid ion exchange resin; (3) passing through a 4A molecular sieve; and (4) microfiltering. The firstly added strong alkali solid can react with unsaturated nitrile and other impurities containing ultraviolet absorbance functional groups in the crude acetonitrile to generate the products with different physical properties from acetonitrile, thereby removing the unsaturated nitrile and other impurities; and the strong-acid ion exchange resin for adsorption is utilized to further remove impurities, the 4A molecular sieve for adsorption is utilized to remove water, and the microfiltration is utilized to remove the doped treating agents used in the three previous steps. Compared with the method comprising distillation, the method disclosed by the invention can save time, and the purified product has higher purity and higher yield.
Owner:TAICANG HUSHI REAGENT

Cord strapping method

The invention discloses a cord strapping and knot tying method, which comprises the following steps: pre-winding multiple cord knots on a support in the shape of a hollow cylinder or other shapes by various required strapping methods and knot tying methods, and placing the cord knots on the object parts needing to be strapped. The free end of the cord is wound around an object needing to be strapped and pulled back by a nipper or other devices passing through the inside of the support so that the free end of the cord and the nipper pass through the centers of multiple cord knots pre-wound on the surface of the support in the shape of a hollow cylinder or other shapes; a pair of pushing tongs or a pushing rod pushes the multiple cord knots pre-wound on the support one by one along the knot tying direction of the cord knots so that the cord knots are wound and knotted with the free end of the cord wound around the strapped object; and the core knots are pushed to the object needing to be strapped and firmly tied to realize an effective strapping and knot tying effect.
Owner:苏红 +1

One-step preparation method of metal oxide at chlorine doped graphene anode material for lithium ion battery

A method for prepare a metal oxide at chlorine doped graphene lithium ion battery cathode material in one step belong to that preparation field of a lithium ion battery cathode. The invention solves the problem of poor consistency of negative electrode material caused by complex traditional coating process. 1 uniformly disperse graphene oxide (GO) in an organic solvent to obtain GO slurry; 2, dissolve a metal source in an organic solvent, slowly adding that metal source into a GO slurry, and stir at room temperature for at least 10 hours to obtain a gel; 3, uniformly coat that gel on the copper foil, dry, cutting, and calcining under the protection of an inert gas to obtain a metal oxide at chlorine doped graphene composite material. The invention utilizes a simple process to prepare the negative electrode material at a low temperature in one step, thereby greatly improving the cycle stability of the negative electrode material; During discharge at 0.2 C, the discharge capacity is up to 600mAh g-1, no attenuation after 80 turns.
Owner:HARBIN INST OF TECH

Triangle optimization method for separating target object in complex object by using simulated moving bed chromatography

ActiveCN106596761AThe apparent adsorption coefficient is accurateSimplify the optimization processComponent separationProcess dynamicsSimulated moving bed
The invention discloses a triangle optimization method for separating a target object in a complex object by using a simulated moving bed chromatography, which belongs to the technical field of chromatography. The method comprises the following steps: determining a interfering component of the target object in a SMB separation system, endowing the concentration of the interfering component, then establishing a single column ideal chromatogram model, obtaining adsorption isotherm of the target object and the interfering component of a SMB separation unit, which is closer to the chromatogram process dynamic characteristic, obtaining the apparent adsorption coefficients of a fast component A and a slow component B, controlling the Relative flow rate ratio m alpha of a II band and a III band of SMB is in a triangle area formed by the apparent adsorption coefficients of the fast component A and the slow component B, and can optimizing the operation parameter of the target object and the interfering component.
Owner:UNIV OF SCI & TECH LIAONING

Method for realizing self-pollination of maize plant by isolation of exogenous pollen with net bag

InactiveCN107173218AHigh reproduction qualityGood light transmission and air permeabilityPlant genotype modificationSelf-pollinationTassel
The invention discloses a method for self-crossing corn plants by isolating exogenous pollen with net bags. In the method, a net bag with a mesh diameter smaller than that of corn pollen is placed on the corn plants before the ear of the corn plants is silked. The mouth of the bag is fixed on the corn stalk, and the fixed position is below the ear. The net bag is fixed on the iron frame above the net bag through the rope at the bottom of the bag, and a certain distance is left between the bottom of the bag and the tassel. The net bag of the present invention is made of nylon material, has good light transmission and air permeability, can be reused, has a simple structure, is convenient to use, and has a good effect; it can completely isolate pollen, is not easy to cause external source pollution, and significantly improves the breeding quality of inbred lines; The traditional artificial bagging method needs to observe the silking situation of the ear of the corn plant, but the present invention can cover the net bag on the corn plant earlier before the ear of the ear is spinning, so the method is time-saving and labor-saving.
Owner:陕西稻香网络科技有限公司

Graphene/boron nitride fiber composite and preparation method thereof

The invention discloses a graphene / boron nitride fiber composite and a preparation method of the graphene / boron nitride fiber composite, and a boron nitride fiber is crosslinked through the swelling and fusing actions of a graphene oxide coating on the surface of the boron nitride fiber. The graphene oxide serves as a sizing agent and a crosslinking agent, the boron nitride fiber is evenly coated,the crosslinking process is rapid and simple, and the crosslinking strength is high. A graphene oxide crosslinking layer is transformed into a graphene crosslinking layer through the further reduction, so that the mutual action between the boron nitride fibers is reinforced, the contact resistance between the fibers is reduced, and the boron nitride fiber shows an excellent mechanical property and electric conduction and heat conduction properties, and can be used in a reinforced phase of the composite. The method for crosslinking the boron nitride fiber through the graphene has a huge research value and a broad application prospect.
Owner:HANGZHOU GAOXI TECH CO LTD

Method for transient expression of target protein by 293T cell and application thereof

The invention provides a method for transient expression of target protein by 293T cells and application thereof, and the method comprises the following steps: S10: obtaining target DNA and 293T cells, and transfecting to obtain transfected 293T cells; s20, culturing the transfected 293T cells, and adding a culture medium and a histone deacetylase inhibitor and / or copper ions; s30, maintaining the culture temperature to be lower than 37 DEG C, and supplementing the material to obtain a 293T cell supernatant after material supplementation; and S40, purifying the 293T cell supernatant after material supplementation to obtain the target protein. The technical problem of high production cost caused by low transient expression level of the current recombinant protein in 293T cells is solved, and the technical effects of improving the transient expression level of the recombinant protein in the 293T cells and reducing the production cost are achieved.
Owner:NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD

Method for detecting glutathione by using fluorescence enhancement method

The invention relates to a method for detecting glutathione by using a fluorescence enhancement method, which comprises the following steps: (1), putting a fluorescent material and glutathione into water, and uniformly mixing the mixture; (2), adding a surfactant, cations and anions into the mixed solution to form a glutathione fluorescence detection system; (3), uniformly mixing the prepared fluorescence detection system, and placing the mixture in an environment of 20-40 DEG C for detection; and (4), irradiating the fluorescence detection system by adopting 980nm excitation, and recording a fluorescence intensity F value at 550nm to obtain a product. According to the method, the characteristic that the up-conversion nano material can emit visible light under the excitation of near-infrared light is utilized, and the fluorescence of the up-conversion nano material can be enhanced in a system containing a surfactant, cations and anions, so that glutathione in the system is detected by high-sensitivity sensing, and the method is high in selectivity and good in stability.
Owner:TIANJIN UNIV OF SCI & TECH

A Triangular Optimization Method for the Separation of Targets from Complex Compounds by Simulated Moving Bed Chromatography

ActiveCN106596761BThe apparent adsorption coefficient is accurateSimplify the optimization processComponent separationProcess dynamicsSimulated moving bed
The patent discloses a triangular optimization method for separating targets in complex substances by simulated moving bed chromatography, which belongs to the technical field of chromatography. This method first determines the interfering components of the target in the SMB separation system, and assigns the concentration of the interfering components, then establishes a single-column ideal chromatographic model, and obtains the adsorption isotherm of the target and interfering components of the SMB separation unit through the inverse method. It is closer to the dynamic characteristics of the chromatographic process, and thus obtains the apparent adsorption coefficient of fast component A and slow component B, and controls the relative flow rate ratio m of band II and band III of SMB α In the triangular region formed by the apparent adsorption coefficients of fast component A and slow component B, the operating parameters of nonlinear SMB separation of target components and interference components can be optimized.
Owner:UNIV OF SCI & TECH LIAONING

One-step preparation of carbon nitride/titanium dioxide heterojunction photocatalyst by electrospinning

The invention belongs to the technical field of photocatalysts for organic sewage degradation, and particularly relates to a method for preparing carbon nitride / titanium dioxide heterojunction photocatalysts by one-step electrospinning. The heterojunction photocatalyst is prepared by a one-step electrospinning method. Specifically, melamine or guanidine hydrochloride or urea is added to a mixed solution containing glacial acetic acid, tetrabutyl titanate, polyvinylpyrrolidone and an organic solvent, and electrospinning is performed. The carbon nitride / titanium dioxide heterojunction photocatalyst is prepared by the process and high temperature calcination treatment. The prepared photocatalyst has a good heterostructure and excellent photocatalytic degradation performance of organic sewage, which simplifies the preparation process of carbon nitride / titanium dioxide heterojunction photocatalyst, and the process is simple and time-saving. It has better application prospects.
Owner:湖北省亨德利医疗器械有限公司

One-pot dispersion polymerization for the preparation of non-spherical, raspberry-like or hollow polymer microspheres

The invention provides a method for preparing nonspherical, raspberry-shaped or hollow polymer microspheres through one-pot dispersion polymerization, and belongs to the technical field of dispersion polymerization. The method comprises the steps of stirring monomers styrene St and sodium styrenesulfonate NaSS and an initiator azodiisobutyronitrile AIBN, and dissolving the mixture in the mixed solution of an organic solvent and water to form a reaction solution; increasing the temperature of the reaction solution to the range from 75 to 80 DEG C and reacting for a certain of time; next, dropwise adding a cross-linking agent or the cross-linking and other reaction reagents to the reaction system, and further reacting for a while to obtain nonspherical, raspberry-shaped or hollow polymer microspheres. According to the invention, a method for directly preparing different types (nonspherical, raspberry-shaped or hollow) of polymer microspheres based on conventional dispersion polymerization and by controlling the cross-linking reaction process and adjusting reaction parameters is established.
Owner:BEIJING UNIV OF CHEM TECH

Construction method and detection method of uplc characteristic map of Angelica dahurica

The invention relates to a construction method and detection method of UPLC characteristic map of Angelica dahurica medicinal materials. The method comprises the following steps: preparation of a reference substance solution: weighing the imperatorin reference substance, adding a solvent to dissolve to obtain a reference substance solution; preparation of a test solution: weighing the medicinal material of Angelica dahurica, adding an extraction solvent for ultrasonication or reflux Extract, filter, and get the continued filtrate to obtain the described need testing solution; the establishment of the characteristic spectrum: absorb the described reference substance solution and the need testing solution respectively, inject into an ultra-high performance liquid chromatograph, and measure to obtain the described test solution. Characteristic spectrum; wherein the elution conditions adopted by the ultra-high performance liquid chromatography are: acetonitrile is used as mobile phase A, and 0.05-0.15% acetic acid aqueous solution is used as mobile phase B with a volume concentration; gradient elution is adopted. The characteristic map established by this method can reflect the characteristics of Angelica dahurica more comprehensively, and the method is accurate, reliable, reproducible and easy to operate.
Owner:GUANGDONG YIFANG PHARMA

Primer probe set, kit and method for real-time fluorescent PCR detection of African swine fever virus cd2v gene

The invention belongs to the technical field of molecular biology, and discloses a real-time fluorescent PCR (polymerase chain reaction) detection primer probe set, kit and method for African swine fever virus CD2V gene A nucleotide sequence of a forward primer of the primer probe set is shown as SEQ ID NO. 4, a nucleotide sequence of a reverse primer is shown as SEQ ID NO. 5, and a nucleotide sequence of a specific fluorescent probe is shown as SEQ ID NO. 6, wherein a fluorescent reporter group is marked at the 5' end of the specific fluorescent probe, while a fluorescent quenching group is marked at the 3' end of the specific fluorescent probe. The primer probe set and kit provided by the invention help quickly, conveniently, efficiently and specifically detect whether a sample containsAfrican swine fever virus and whether the sample is identified as CD2V gene deletion, and are suitable for clinical identification detection.
Owner:南宁壮博生物科技有限公司

Primers for cloning SPFMV (sweet potato feathery mottle virus) O strain and full length genome sequence of SPVC (sweet potato virus C) and cloning method

The invention discloses primers for cloning SPFMV (sweet potato feathery mottle virus) O strain and full length genome sequence of SPVC (sweet potato virus C). The primers are respectively a primer for cloning the SPFMV O strain, a primer for cloning the full length genome sequence of SPVC, and the primer for simultaneously cloning the SPFMV O strain and the full length genome sequence of SPVC. The SPFMV and the SPVC generally infect sweet potatoes in a mixed way, the separation and the purification are very difficult; the content of the virus in the sweet potatoes is low; the full length genome sequence is difficult to obtain by a conventional virus purification cloning method. The principle that SPCSV can promote the content rise of the SPFMV and the SPVC in the sweet potato plants is used; the sweet potato plants simultaneously infected with SPCSV, SPFMV-O and SPVC viruses are used as materials; the total RNA (ribonucleic acid) of the virus-infecting sweet potato plant leaves is extracted; the virus full length genome sequence cloning is performed; through a great amount of PCR (polymerase chain reaction) amplification and sequence testing, the genome full length sequence of SPVC and SPFMV is obtained through comparison and splicing.
Owner:INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI

Cell line for detecting activity of human interferon-kappa and construction and detection method thereof

The invention discloses a cell line for detecting the activity of human interferon-kappa and a construction and detection method thereof. The cell line is obtained by transfecting eukaryotic cells with ISRE (interferon-sensitive response element) luciferase reporter genes and stably screening monoclonal cell lines. The cell line constructed by the invention is easy to culture and amplify and good in passage stability, and has the advantages of high sensitivity, short detection time, completion within 4-6 hours, and capability of greatly improving the activity release efficiency of the human interferon-kappa in the production process.
Owner:SHANDONG RUIYING PIONEER PHARMA

Method for taking honey without injuring brood combs in movable frame breeding of bees

The invention relates to the technical field of bee breeding, in particular to a method for taking honey without injuring brood combs in movable frame breeding of bees. The method comprises the stepsof cutting combs, namely, selecting the honey flowing season of the bees, on a movable frame, due to the fact that honey combs of the bees are fully distributed on the upper portion and the middle-upper portion of the movable frame, and powder combs and the brood combs are arranged at the middle portion and the lower portion of the movable frame, cutting down the honey combs at the upper portion of the movable frame through a knife, and keeping the brood combs and the powder combs against damage; 2, preparing a base, namely taking pure beeswax, and preparing a pure beeswax nest base accordingto the size and shape of the honey combs cut at the lower portion; and 3, supplementing the base, namely supplementing the prepared pure beewax on the portion, where the honey combs are cut off, of the movable frame, and smearing a small amount of honey at the joint, and feeding the bees with the honey taken out from the cut honey combs. The method for taking the honey without injuring the brood combs in movable frame breeding of the bees is provided.
Owner:贵州懒汉蜜蜂养殖场
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