51results about How to "Method saves time" patented technology

The invention relates to a method for preparing biodegradable polyester. Firstly, melt polycondensation is conducted through diol and dicarboxylic acid and a low molecular weight polyester prepolymer with number average molecular weight between 2, 000 and 20, 000 or intrinsic viscosity below 0.64dL/g is prepared; binary oxadiazoline and diacyl bis-caprolactamate are combined to extend chain, and chain extension is carried out at a certain temperature; and the biodegradable polyester is obtained. The number average molecular weight of polybutylene adipate (PBA) can reach up to 39, 000 and the intrinsic viscosity to 1.13dL/g. For poly (butylene succinate) (PBS) with relatively high melting point, the intrinsic viscosity after chain extension is up to 0.99dL/g.

The invention discloses a preparation method for biodegradable aliphatic alternating polyester amide. According to the preparation method, aliphatic dicarboxylic acid and diamide diol are subjected to melt phase polycondensation to prepare alternating polyester amide prepolymer with terminal carboxyl group and terminal hydroxy group; and diacyl di-lactam and a bisoxazoline chain extender are subjected to joint chain extension to prepare the biodegradable aliphatic alternating polyester amide with the intrinsic viscosity of 0.30-0.59dL/g. The preparation method disclosed by the invention has the characteristics of simple and convenient method, mild conditions, high efficiency and the like and can be used for preparing the biodegradable aliphatic alternating polyester amide with regular structure and favorable crystallinity.

The invention discloses a graphene-basalt fiber composite material and a preparation method thereof. The cross-linking of the basalt fiber can be achieved by utilizing the swelling and fusion effectsof graphene oxide coating on the surface of the basalt fiber. The graphene oxide is taken as a sizing agent and a cross-linking agent, the basalt fiber can be evenly coated with the grapheme oxide, the cross-linking process is fast and easy, and the cross-linking intensity is high. After further deoxidization, a grapheme oxide cross-linking layer converts into a graphene cross-linking layer, the interaction between the basalt fibers is enhanced, the contact resistance between fibers is decreased, so that the basalt fiber demonstrates excellent mechanical property and electric and thermal conductivity, which can be used for reinforcement phase of the composite material and differentiated fiber application. The method of cross-linking the basalt fiber with the graphene is of great research value and extensive application prospect.

The invention relates to a multiple reverse transcription polymerase chain reaction detection method for swine transmissible gastroenteritis. An antibody is needed to be prepared by an euzymelinked immunosorbent assay, so that the period is longer, and external factors have larger interference on the diagnostic method of the euzymelinked immunosorbent assay. The method comprises the following steps of: designing and synthesizing a transmissible swine gastroenteritis virus gene primer; establishing a multiple reverse transcription polymerase chain reaction diagnostic method of the transmissible swine gastroenteritis viruses and porcine epidemic diarrhea viruses; optimizing concentration of the primer of a bigeminy reverse transcription polymerase chain reaction; optimizing the concentration of magnesium oxide of the bigeminy reverse transcription polymerase chain reaction; optimizing the concentration of triphosphoric acid base deoxynucleotide of the bigeminy reverse transcription polymerase chain reaction; determining the optimal annealing temperature of the bigeminy reverse transcription polymerase chain reaction; testing the sensitivity of the bigeminy reverse transcription polymerase chain reaction; and testing the specificity of the bigeminy reverse transcription polymerase chain reaction. The multiple reverse transcription polymerase chain reaction detection method for swine transmissible gastroenteritis is suitable for detecting swine transmissible gastroenteritis.

The invention discloses a graphene-glass fiber composite material and a preparation method thereof. Crosslinking of glass fibers can be realized by means of the swelling and fusing actions of grapheneoxide coatings on the surfaces of the glass fibers. Graphene oxide is taken as a sizing agent as well as a crosslinking agent with which the glass fibers can be coated uniformly, the crosslinking process is rapid and easy, and high crosslinking strength is achieved. Through further reduction, a graphene oxide crosslinking layer is turned into a graphene crosslinking layer, so that mutual action among the glass fibers is enhanced, contact resistance among the fibers is lowered, and the glass fibers show excellent mechanical performance, electric conductivity and heat conductivity and can be applied to a wild phase of the composite material. The method for crosslinking the glass fibers with the graphene has a great researching value and a wide application prospect.

The invention relates to a biodegradable alternate poly tetraamide diester preparation method, and belongs to the technical field of polyamide esters. The preparation method comprises that: an aliphatic diamine and a dicarboxylic acid ester are subjected to a reaction to prepare a diamide diester, the diamide diester reacts with alcohol amine to obtain tetraamide diol, the tetraamide diol and a dicarboxylic acid are subjected to condensation polymerization to prepare an alternate poly tetraamide diester prepolymer, and chain extenders such as bisoxazoline and diacyl di-lactam are adopted to carry out chain extension on the prepolymer to prepare the biodegradable alternate poly tetraamide diester with the 30 DEG C intrinsic viscosity of 0.26-0.45 dL/g. The biodegradable alternate poly tetraamide diester has characteristics of regular structure, good crystallinity, high melting point, good thermal property, good mechanical property, and green and pollution-free synthesis route.

The invention discloses an acetonitrile purification method. The purification method comprises the following steps: (1) adding a strong alkali solid into crude acetonitrile to be treated to perform pretreatment; (2) passing through a strong-acid ion exchange resin; (3) passing through a 4A molecular sieve; and (4) microfiltering. The firstly added strong alkali solid can react with unsaturated nitrile and other impurities containing ultraviolet absorbance functional groups in the crude acetonitrile to generate the products with different physical properties from acetonitrile, thereby removing the unsaturated nitrile and other impurities; and the strong-acid ion exchange resin for adsorption is utilized to further remove impurities, the 4A molecular sieve for adsorption is utilized to remove water, and the microfiltration is utilized to remove the doped treating agents used in the three previous steps. Compared with the method comprising distillation, the method disclosed by the invention can save time, and the purified product has higher purity and higher yield.

The invention discloses a triangle optimization method for separating a target object in a complex object by using a simulated moving bed chromatography, which belongs to the technical field of chromatography. The method comprises the following steps: determining a interfering component of the target object in a SMB separation system, endowing the concentration of the interfering component, then establishing a single column ideal chromatogram model, obtaining adsorption isotherm of the target object and the interfering component of a SMB separation unit, which is closer to the chromatogram process dynamic characteristic, obtaining the apparent adsorption coefficients of a fast component A and a slow component B, controlling the Relative flow rate ratio m alpha of a II band and a III band of SMB is in a triangle area formed by the apparent adsorption coefficients of the fast component A and the slow component B, and can optimizing the operation parameter of the target object and the interfering component.

The invention discloses a method for self-crossing corn plants by isolating exogenous pollen with net bags. In the method, a net bag with a mesh diameter smaller than that of corn pollen is placed on the corn plants before the ear of the corn plants is silked. The mouth of the bag is fixed on the corn stalk, and the fixed position is below the ear. The net bag is fixed on the iron frame above the net bag through the rope at the bottom of the bag, and a certain distance is left between the bottom of the bag and the tassel. The net bag of the present invention is made of nylon material, has good light transmission and air permeability, can be reused, has a simple structure, is convenient to use, and has a good effect; it can completely isolate pollen, is not easy to cause external source pollution, and significantly improves the breeding quality of inbred lines; The traditional artificial bagging method needs to observe the silking situation of the ear of the corn plant, but the present invention can cover the net bag on the corn plant earlier before the ear of the ear is spinning, so the method is time-saving and labor-saving.

The invention provides a method for transient expression of target protein by 293T cells and application thereof, and the method comprises the following steps: S10: obtaining target DNA and 293T cells, and transfecting to obtain transfected 293T cells; s20, culturing the transfected 293T cells, and adding a culture medium and a histone deacetylase inhibitor and/or copper ions; s30, maintaining the culture temperature to be lower than 37 DEG C, and supplementing the material to obtain a 293T cell supernatant after material supplementation; and S40, purifying the 293T cell supernatant after material supplementation to obtain the target protein. The technical problem of high production cost caused by low transient expression level of the current recombinant protein in 293T cells is solved, and the technical effects of improving the transient expression level of the recombinant protein in the 293T cells and reducing the production cost are achieved.

The invention discloses primers for cloning SPFMV (sweet potato feathery mottle virus) O strain and full length genome sequence of SPVC (sweet potato virus C). The primers are respectively a primer for cloning the SPFMV O strain, a primer for cloning the full length genome sequence of SPVC, and the primer for simultaneously cloning the SPFMV O strain and the full length genome sequence of SPVC. The SPFMV and the SPVC generally infect sweet potatoes in a mixed way, the separation and the purification are very difficult; the content of the virus in the sweet potatoes is low; the full length genome sequence is difficult to obtain by a conventional virus purification cloning method. The principle that SPCSV can promote the content rise of the SPFMV and the SPVC in the sweet potato plants is used; the sweet potato plants simultaneously infected with SPCSV, SPFMV-O and SPVC viruses are used as materials; the total RNA (ribonucleic acid) of the virus-infecting sweet potato plant leaves is extracted; the virus full length genome sequence cloning is performed; through a great amount of PCR (polymerase chain reaction) amplification and sequence testing, the genome full length sequence of SPVC and SPFMV is obtained through comparison and splicing.