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Method for realizing self-pollination of maize plant by isolation of exogenous pollen with net bag

A mesh bag and corn technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of labor and time-consuming, easy to fall off, difficult to fix the paper bag, etc. Use good effects

Inactive Publication Date: 2017-09-19
陕西稻香网络科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for self-fertilization of corn plants by isolating exogenous pollen in net bags, aiming at solving the cumbersome operation of ordinary artificial pollination, which is labor-intensive and time-consuming; When it rains, the paper bag is easy to get wet and airtight, which will affect the quality of pollen and affect the pollination situation; and the paper bag cannot be reused, and the use cost is high

Method used

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  • Method for realizing self-pollination of maize plant by isolation of exogenous pollen with net bag
  • Method for realizing self-pollination of maize plant by isolation of exogenous pollen with net bag
  • Method for realizing self-pollination of maize plant by isolation of exogenous pollen with net bag

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment one, test the effect of net bag isolation pollen

[0017] Test materials: There are 3 kinds of corn varieties for the test, a total of 60 plants. When entering the flowering period, select the plants with good growth and normal development to carry out the test. Choose 4 different specifications of nylon mesh bags, which are 120 mesh, 200 mesh, 300 mesh, and 400 mesh four mesh densities.

[0018] Test method: The test was carried out in the self-crossing test field. After the corn plants grew ears and before the filaments were spit out, the ears of each corn variety were respectively bagged with 4 kinds of mesh bags with 5 plants, and the mouth of the bag was tied tightly with a rope. After the ear is silked, each corn plant sprinkles a large amount of pollen collected in a paper bag on the outer ear of the net bag. After the corn plants bear fruit, count the number of fruit seeds respectively.

[0019] results and analysis:

[0020]

[0021] The stat...

Embodiment 2

[0022] Embodiment two, test the effect of mesh bag selfing

[0023] Test materials: There are three kinds of corn varieties for the test. A total of 90 plants. When entering the flowering period, select the plants with good growth and normal development to carry out the test. 45 300-mesh pollen isolation mesh bags and 90 sodium sulfate paper bags.

[0024] Test method: The test was carried out in the self-crossing test field. 15 corn plants of each variety of the three varieties were divided into two groups for testing, and one group was self-crossed by this method, that is, a mesh bag was used, and the mesh bag was placed on the corn plants before the ear of corn was silked. Shake the corn plants periodically when the tassels begin to shed flour and the ears begin to silk. The other group used the common artificial bagging method for selfing, that is, paper bags. When the ears of the selected plants were about to spin silk but were not silking, the ear ears were covered w...

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Abstract

The invention discloses a method for self-crossing corn plants by isolating exogenous pollen with net bags. In the method, a net bag with a mesh diameter smaller than that of corn pollen is placed on the corn plants before the ear of the corn plants is silked. The mouth of the bag is fixed on the corn stalk, and the fixed position is below the ear. The net bag is fixed on the iron frame above the net bag through the rope at the bottom of the bag, and a certain distance is left between the bottom of the bag and the tassel. The net bag of the present invention is made of nylon material, has good light transmission and air permeability, can be reused, has a simple structure, is convenient to use, and has a good effect; it can completely isolate pollen, is not easy to cause external source pollution, and significantly improves the breeding quality of inbred lines; The traditional artificial bagging method needs to observe the silking situation of the ear of the corn plant, but the present invention can cover the net bag on the corn plant earlier before the ear of the ear is spinning, so the method is time-saving and labor-saving.

Description

technical field [0001] The invention belongs to the field of corn breeding, and in particular relates to a method for isolating exogenous pollen in a mesh bag to realize selfing of corn plants. Background technique [0002] Maize is a monoecious plant with different flowers. The tassels are born on the top of the stem of the plant, and the ears are born on the nodes in the middle of the stem. The cross-pollination rate is over 95%. In order to self-pollinate corn varieties without being mixed with other types of pollen, artificial bagging is usually used to isolate pollen from other sources in order to obtain purer self-pollination seeds. The common artificial bagging method is: when entering the flowering period, select a well-developed and normal plant to carry out bagging self-breeding. When the ear of the selected plant is about to spin silk but not silk, the ear is covered with a sodium sulfate paper bag; when the ear is silked in the afternoon of the first day, the ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 张德凤
Owner 陕西稻香网络科技有限公司
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