41 results about "Full length genome" patented technology

An allergen-free transgenic peanut seed is produced by recombinant methods. Peanut plants are transformed with multiple copies of each of the allergen genes, or fragments thereof, to suppress gene expression and allergen protein production. Alternatively, peanut plants are transformed with peanut allergen antisense genes introduced into the peanut genome as antisense fragments, sense fragments, or combinations of both antisense and sense fragments. Peanut transgenes are under the control of the 35S promoter, or the promoter of the Ara h2 gene to produce antisense RNAs, sense RNAs, and double-stranded RNAs for suppressing allergen protein production in peanut plants. A full length genomic clone for allergen Ara h2 is isolated and sequenced. The ORF is 622 nucleotides long. The predicted encoded protein is 207 amino acids long and includes a putative transit peptide of 21 residues. One polyadenilation signal is identified at position 951. Six additional stop codons are observed. A promoter region was revealed containing a putative TATA box located at position −72. Homologous regions were identified between Ara h2, h6, and h7, and between Ara h3 and h4, and between Ara h1P41B and Ara h1P17. The homologous regions will be used for the screening of peanut genomic library to isolate all peanut allergen genes and for down-regulation and silencing of multiple peanut allergen genes.

The invention discloses a restructured infectious cloning plasmid of a low virulent strain of a Japanese encephalitis virus, which contains a full-length genome cDNA sequence of the low virulent strain 10S3 of the Japanese encephalitis virus, or a full-length cDNA sequence of a marked cleavage site introduced in the full-length genome cDNA sequence of the low virulent strain 10S3 of the Japanese encephalitis virus. The invention also discloses an infectious cloning low virulent strain of the Japanese encephalitis virus. The low virulent strain is a cloning strain of the low virulent strain 10S3 of the Japanese encephalitis virus, and the full-length genome cDNA sequence of the low virulent strain 10S3 is shown in SEQ ID NO.1. The restructured infectious cloning plasmid of the low virulent strain of the Japanese encephalitis virus and the infectious cloning low virulent strain of the Japanese encephalitis virus rescued from cells can provide complete immunoprotection for immunized animals resisting Japanese encephalitis viruses, thereby extremely facilitating the prevention and control of Japanese encephalitis viruses.