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Rabies virus Flury-LEP vaccine strain reverse genetic operating system and LEP green fluorescent protein recombination viral vector

A green fluorescent protein, rabies virus technology, applied in the direction of virus/phage, antiviral agent, recombinant DNA technology, etc., can solve the problem that there is no reverse genetic operating system of Flury-LEP strain

Inactive Publication Date: 2009-11-25
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the reverse genetic operating system of Flury-LEP strain at home and abroad

Method used

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  • Rabies virus Flury-LEP vaccine strain reverse genetic operating system and LEP green fluorescent protein recombination viral vector
  • Rabies virus Flury-LEP vaccine strain reverse genetic operating system and LEP green fluorescent protein recombination viral vector
  • Rabies virus Flury-LEP vaccine strain reverse genetic operating system and LEP green fluorescent protein recombination viral vector

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Experimental program
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Embodiment 1

[0022] Example 1 Rabies virus Flury-LEP strain reverse genetic operating system and its expression green

[0023] Construction and biological activity of recombinant virus vector of fluorescent protein

[0024] 1 material

[0025] 1.1 Plasmids, cell lines and viruses

[0026] Plasmids pCAGG and pCI-RBz were purchased from Harbin Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences, and pBluescriptII SK(+) was purchased from clonetech. BHK-21 cells (ATCC CCl-10) and 293T cells (CRL-11268) were purchased from ATCC. The culture medium was DMEM containing 10% fetal bovine serum. Mouse brain neuroma cells were purchased from the military veterinarian of the Academy of Military Medical Sciences. In the institute, the culture medium is MEM with 10% fetal bovine serum, and the rabies virus Flury-LEP was purchased from the China Veterinary Drug Administration, amplified on BHK-21 cells, and frozen at -70°C for use.

[0027] 1.2 Main reagents and instruments

[0028] T...

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Abstract

The invention relates to a rabies virus Flury-LEP vaccine strain reverse genetic operating system comprising a recombination vector for coding LEP full length genome cDNA and an auxiliary plasmid system thereof, wherein, the auxiliary plasmid system respectively codes nucleoprotein of LEP, phosphoric acid protein P and polyase protein L. the invention also relates to LEP green fluorescent protein recombination viral vector built by the reverse genetic operating system, concretely, the rabies virus Flury-LEP vaccine strain reverse genetic operating system is pCI-LEP and auxiliary plasmid systems pCAGG-N, pCAGG-P and pCAGG-L, the LEP green fluorescent protein recombination viral vector is pCI_LEP_EGEP. The invention also relates to recombination viruses rescued by the rabies virus Flury-LEP vaccine strain reverse genetic operating system and the LEP green fluorescent protein recombination viral vector, and applications thereof.

Description

Technical field [0001] The present invention relates to the field of recombinant virus vaccines. More specifically, the present invention relates to a rabies virus Flury-LEP vaccine strain reverse genetic operating system, which includes a recombinant vector encoding LEP full-length genomic cDNA, and an auxiliary plasmid system, wherein the auxiliary plasmid system The plasmid system respectively encodes the nucleoprotein N, phosphoprotein P and polymerase protein L of LEP. The invention also relates to the LEP green fluorescent protein recombinant virus vector constructed by the reverse genetic operating system. More specifically, the rabies virus Flury-LEP vaccine strain reverse genetic operating system is pCI-LEP and its auxiliary plasmid systems pCAGG-N, pCAGG-P and pCAGG-L, and the LEP green fluorescent protein recombinant viral vector is pCI -LEP-EGFP. The invention also relates to recombinant viruses rescued by the rabies virus Flury-LEP vaccine strain reverse genetic opera...

Claims

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Application Information

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IPC IPC(8): C12N15/85C12N7/01A61K39/205A61P31/14C12Q1/70C12R1/93
Inventor 步志高陶丽红葛金英王喜军
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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