21 results about "Neuroma" patented technology

Methods, devices and systems are described for gel-based modulation of neural tissue, including prevention of nerve regeneration and neuroma formation. The gel can be delivered to selected target locations within or proximate nerves, including interfascicularly and intrafascicularly. Gel delivery associated with an operative procedure for the treatment of pain and other indications is also disclosed.

The invention provides a method for in-vitro detection of exogenous gene expression activity of AAV virus in nerve cells and an evaluation system. The method comprises the following steps: infecting N2A cranial nerve tumor cells by using AAV carrying a neuron-specific promoter or a glial cell-specific promoter and a reporter gene driven by the neuron-specific promoter or the glial cell-specific promoter; and detecting the level of a reporter gene transcript or a protein expressed by the reporter gene in the infected N2A cranial nerve tumor cell so as to detect the activity of the AAV expressing the exogenous gene. According to the method disclosed by the invention, the target gene expression driven by the CamKII promoter of the AAV9 serotype is tested by utilizing the N2A cranial nerve tumor cells, the problem that a suitable cell model is lacked in the prior art is solved, and a new tool and a new method are provided for AAV activity test in neurobiology research.

The application discloses a waterproof and breathable type brain and spinal cord nerve injury wound protection dressing, which is sequentially stacked and arranged from inside to outside as a wound adhesion inner layer, a functional sustained-release middle layer and a waterproof and breathable outer layer; the three-layer synergistic dressing structure of the wound adhesion inner layer, the functional sustained-release middle layer and the waterproof and breathable outer layer is constructed, waterproof sealing and the dynamic adaptation of the air permeability performance of the brain and spinal cord nerve injury wound are realized, the target anti-scar polypeptide is covalently bonded on the inner layer, the effective inhibition of excessive scar hyperplasia and neuroma formation is realized, the matching regulation of the gradient degradation drug-loaded microsphere system and the air permeation pore size of the middle layer is realized, the accurate release of active factors in the whole cycle of nerve repair is realized, the long-acting waterproof and breathable protection in a dynamic scene is realized through the design of the outer layer of super-hydrophobic nanofiber membrane, the risk of postoperative complications is reduced, and the integration and synergy of the physical protection of the wound and the nerve function repair are realized.

The invention relates to a nerve conduit for connecting an impaired nerve ending to a target site. In particular, the present invention relates to nerve conduits that support nerve injury repair and motion control and / or reduce the risk of developing a neuroma at an impaired nerve ending. Thus, a nerve conduit (10) for connecting a nerve ending to a target site is proposed, comprising an elongated body (12) formed by a wall (22) defining an internal cavity (16), and comprising: a central portion (14) wherein the internal cavity (16) extends through the central portion (14); and two end portions (18, 19) defining respective openings (20, 21) to the lumen (16), one end portion (18) being an insertion portion configured for insertion into a respective nerve ending and the other end portion (19) being a nerve ending exit portion. According to the invention, the end portions (18, 19) are arranged such that the cross-sections of the openings (20, 21) are oriented at an angle to one another.

The invention provides a Janus membrane with tissue adhesiveness, a preparation method of the Janus membrane and application of the Janus membrane to neural restoration. The Janus membrane with the tissue adhesiveness comprises an electrospun fiber layer and an oriented hydrogel fiber layer with the tissue adhesiveness. The electrospun fiber layer is used as a non-viscous isolation layer, has good mechanical properties and air permeability, and can effectively avoid adhesion and compression of surrounding tissues; the oriented hydrogel fiber layer has unique tissue adhesiveness and an oriented structure, the tissue adhesiveness characteristic of the oriented hydrogel fiber layer can ensure that the oriented hydrogel fiber layer is tightly attached to nervous tissues, and the oriented structure can guide directional regeneration of nerves, so that the nerve regeneration efficiency is remarkably improved, directional adhesion and migration of cells can be induced, axons are promoted to extend in a specific direction, and the nerve regeneration effect is improved. Therefore, the formation of neuroma is effectively prevented. The Janus membrane and a nerve broken end are bonded without tension and curled to form an exclusive catheter which is adaptive to various nerve diameters and complex branch structures.

A composition comprising a liquid plasma according to the present invention can induce growth inhibition and death of brain tumor cells through the combined effect of radicals, ionic species, and pH effect, and thus can be effectively used for preventing or treating brain tumors. In addition, the present invention is a non-invasive physical and chemical treatment approach with low drug dependence, can exhibit a synergistic effect when used in combination with existing anticancer drugs, and has a high potential for application to the treatment of various nervous system tumors or metastatic brain diseases including glioblastoma and auditory nerve tumors.

The invention relates to the technical field of bioengineering, in particular to a culture medium and a method for constructing auditory neuroma organs. According to the culture medium, Neurobase Medium and DMEM (Dulbecco Modified Eagle Medium) / F12 are used as a basic culture medium; the feed additive comprises a N2 additive, a B27 additive, L-alanyl-L-glutamine, an antibiotic, HEPES, glucose, beta-mercaptoethanol, a cell growth factor, MEM NEAA and a TGF-beta inhibitor. The cell growth factor is EGF (Epidermal Growth Factor) or FGF-2. The culture medium provided by the invention can effectively improve the construction efficiency of the auditory neuroma organoid, and the constructed auditory neuroma organoid can be used as a reliable in-vitro substitution model, is used for marker detection, drug screening and individualized medical exploration in basic research and clinical transformation research, and has important application value.

Tissue grafts with reduced regenerative potential, methods of preparing such grafts, and related kits and methods of treatment are described. The method may include treating tissue with a digestion solution comprising trypsin, alpha-chymotrypsin (ACT) and optionally ethylenediaminetetraacetic acid (EDTA) to substantially remove one or more susceptible proteins from the tissue. The method may also include washing the treated tissue with a buffer solution and / or with a serine-containing serum. Nerve grafts prepared according to the disclosed methods may inhibit, or lessen (e.g., provide for reduced) neuroma formation and / or axonal outgrowth after implantation.

The invention provides a nerve conduit, which comprises a cannula, one end of the cannula is provided with an opening, and the other end of the cannula is provided with a folding part; the shape of the filling block is matched with the internal shape of the sleeve; when the filling block is assembled in the sleeve, the distance between one end, close to the opening, of the filling block and one end of the opening of the sleeve is preset; wherein a plurality of through holes are formed in the side wall of the sleeve, and the through holes are configured to provide passages for permeation of tissue fluid; and the filling block is made of a degradable biomedical material. According to the nerve conduit provided by the invention, a protective envelope is formed through the sleeve, replaces an adventitia, coats and limits the growth of nerve tracts, and nerves can be subjected to the action of relative mechanical stress along with the narrowing of the surrounding growth environment, so that the formation of neuroma is more quickly and stably prevented, the extrusion of surrounding muscles in a body is resisted, and the terminal nerves are protected. The nerve conduit is simple in structure and has good mechanical properties, and industrial mass production can be achieved.

The subject invention provides devices and methods for alleviating discomfort associated with neuroma formation. The devices and methods of the invention effectively use the body's natural response of reconstructing implanted biomaterials to minimize the size of, isolate, and protect a neuroma. In preferred embodiments, the subject device is a cylindrical cap, wherein the internal chamber of the cylindrical cap physically partitions the nerve to enable an arrangement of nerve fibers (as opposed to haphazardly arranged nerve fibers often produced in neuromas). Tabs arranged on the outside of the cap can be used to manipulate the cap into place on a nerve. The open end can also be configured with flaps that can be used to widen the open end for easier insertion of the nerve into the cap. In addition, the cap's material remodels into a tissue cushion after implantation, which protects the neuroma from being stimulated and inducing pain.

The invention provides a nerve conduit, which comprises a sleeve, one end of the sleeve is provided with an opening, and the other end of the sleeve is provided with a fixing part; the supporting frame comprises one or more supporting plates; when the supporting frame is assembled in the sleeve, the interior of the sleeve is divided into a plurality of axial limiting channels by the supporting plates, and the limiting channels are configured to provide a preset growth path; a plurality of through holes are formed in the side wall of the sleeve, and the through holes are configured to provide passages for permeation of tissue fluid. According to the nerve conduit provided by the invention, a protective envelope is formed through the sleeve, replaces an adventitia, coats and limits the growth of nerve tracts, and nerves can be subjected to the action of relative mechanical stress along with the narrowing of the surrounding growth environment, so that the formation of neuroma is more quickly and stably prevented, the extrusion of surrounding muscles in a body is resisted, and the terminal nerves are protected. The nerve conduit is simple in structure and has good mechanical properties, and industrial mass production can be achieved.

The present disclosure provides a mechanically-actuated tool for cutting a tissue graft having a hollow core and methods for use thereof. A portion of a biological structure, such as a nerve, is attached to the hollow core to form an implantable neural graft assembly. The tool has a cutter mechanism and a grasper mechanism. The grasper mechanism has one or more component(s) that open and close via an actuation mechanism, like a handle, and rotate via a controller component, like a rotatable wheel. The cutter mechanism may be a cutting tube component that harvests the tissue graft. The tool may also have an ejector mechanism to remove the tissue graft as part of the implantable neural graft assembly. Such devices and methods are particularly suitable for treating neuromas and other neural regeneration procedures.

Disclosed is an individualized nerve conduit with differentiated interfaces, including a hollow outer sheath and an inner core filler disposed inside the hollow outer sheath; wherein a surface of the hollow outer sheath is distributed with nano-scale micropores, and the inner core filler is a loose filler of micron-scale sheets with axially oriented arrangement. The structure of such nerve conduit satisfies the repair microenvironment with different needs, both internal and external, and realizes the important function of “internal affinition and external alienation, internal promotion and external resistance”. The conduit not only promotes directional myelination of nerve and directional ingrowth of axon, but also prevents neuroma formation.

The application discloses a biological adhesive tape for a nerve stump after amputation and a preparation method thereof. The biological adhesive tape comprises an outer layer and an inner layer, and the outer layer and the inner layer are connected by a PEG-based adhesive layer. The outer surface of the outer layer is coated with a PDMS hydrophobic coating layer. The inner surface of the inner layer is also coated with a PEG-based adhesive layer. The outer layer is made of recombinant human collagen type I and contains dexamethasone drug-loaded microspheres. The inner layer is made of recombinant human collagen type I and contains gabapentin drug-loaded microspheres. The outer layer of the biological adhesive tape can inhibit inflammatory response and reduce foreign body reaction by releasing glucocorticoids. The inner layer can prevent and relieve neuropathic pain by releasing gabapentin. The physical adhesion of the adhesive tape can prevent the growth of nerve fibers outward, thereby reducing the risk of neuroma formation. The biological adhesive tape has high flexibility in application, is suitable for nerve stumps with different diameters and shapes, and is suitable for complex trauma.

A nerve conduit for supporting repair of a nerve lesion and motor control and / or reducing risk of the development of a neuroma at the lesioned nerve end. The nerve conduit includes an elongate body formed by a wall and including a central portion defining an inner cavity and end portions defining a respective opening to the inner cavity and arranged adjacent to the central portion and at longitudinally opposing ends of the elongate body. One end portion is configured as an insertion portion configured for inserting a respective nerve end. The other end portion is configured as a nerve end outlet portion, an outer diameter of the wall defining the outlet portion is larger than an outer diameter of the wall defining the central portion.

The invention discloses a nerve end cap of polycaprolactone compounded amorphous calcium phosphate and a preparation method of the nerve end cap, and relates to the technical field of biological materials and medical treatment. The end cap is formed by pressing nanofiber after film forming, and the nanofiber is prepared from a mixed solution of polycaprolactone and amorphous calcium phosphate. The nerve end cap provided by the invention is prepared through a pressurizing process, so that the nerve end cap has good mechanical properties, the preparation process is modularized, the operation is simple, mass production is expected to be realized, and the nerve end cap has good industrial application prospects. The nerve end cap provided by the invention is novel in structure, nerves can be subjected to the action of relative mechanical stress along with the narrowing of the surrounding growth environment, so that the formation of neuroma can be more quickly and stably prevented, and the adopted materials have good biocompatibility, so that the formation of neuroma can be remarkably prevented; and the material has good compressive mechanical properties, resists extrusion of internal surrounding muscles, and protects terminal nerves.

The invention relates to a nerve conduit for connecting an impaired nerve ending to a target site. In particular, the present invention relates to nerve conduits that support nerve injury repair and motion control and / or reduce the risk of development of injured nerve ending neuroma. Thus, a nerve conduit (10) connecting an impaired nerve ending (26) to a target site (28) is proposed, the nerve conduit (10) comprising an elongated body (12) formed by a wall (22) and comprising a central portion (14) defining a lumen (16) and end portions (18, 19) defining respective openings (20, 20 ') to the lumen (16), the end portions (18, 19) being arranged adjacent to the central portion (14) and located at longitudinally opposite ends of the elongated body (12). One tip portion (18) is configured as an insertion portion configured to be inserted into a corresponding nerve ending (26). According to the invention, the other end portion is configured as a nerve ending outlet portion (19) wherein the outer diameter of the wall defining the outlet portion (19) is greater than the outer diameter of the wall defining the central portion (14).

This invention discloses an iridium complex, its preparation method, and its application in the treatment of neuromas. The iridium complex has the following structure: The iridium complex of this invention is a near-infrared fluorescent iridium complex, which exhibits a strong photodynamic therapeutic effect on the mouse neuroma cell line (Neuro-2a cells). Under light irradiation, it strongly inhibits the growth and proliferation of mouse neuroma cells (IC50). 50 The concentration was 0.05 μM, while under dark conditions, its cytotoxicity was only 87.5 μM, and the phototherapy index (PI) was as high as 1750. This is of great significance for the research of metal drugs for the treatment of neuromas.

Tissue grafts with reduced regenerative potential, methods of making such grafts, and related kits and methods of treatment are described. The method can include treating the tissue with a dissociating solution comprising trypsin, alpha-chymotrypsin (ACT), and optionally ethylenediamine tetraacetic acid (EDTA) to substantially remove one or more susceptible proteins from the tissue. The method may also include cleaning the treated tissue with a buffer solution and / or with serine-containing serum. Nerve grafts prepared according to the disclosed methods can inhibit or mitigate (e.g., provide to reduce) neuroma formation and / or axon outgrowth after implantation.

The application belongs to the technical field of in vitro drug evaluation model construction, and particularly relates to a biological ink, an in vitro humanized nerve model and application of the in vitro humanized nerve model in construction of an opioid drug classification prediction model and dose-effect relationship. The biological ink comprises a neuroblastoma cell line, methacrylated gelatin, a photoinitiator and a basement membrane matrix. The in vitro humanized nerve model prepared based on the biological ink provided by the application has microenvironment interaction characteristics of cell-cell and cell-matrix, can more truly restore the complexity of in vivo tissues, can highly bionically simulate the structure and function of human neurons at the cell level, the bionicity is significantly better than traditional two-dimensional culture and animal experiments, and the accuracy and reliability of drug toxicity and dependence evaluation are improved.