13 results about "Neuroma" patented technology

The invention provides a method for in-vitro detection of exogenous gene expression activity of AAV virus in nerve cells and an evaluation system. The method comprises the following steps: infecting N2A cranial nerve tumor cells by using AAV carrying a neuron-specific promoter or a glial cell-specific promoter and a reporter gene driven by the neuron-specific promoter or the glial cell-specific promoter; and detecting the level of a reporter gene transcript or a protein expressed by the reporter gene in the infected N2A cranial nerve tumor cell so as to detect the activity of the AAV expressing the exogenous gene. According to the method disclosed by the invention, the target gene expression driven by the CamKII promoter of the AAV9 serotype is tested by utilizing the N2A cranial nerve tumor cells, the problem that a suitable cell model is lacked in the prior art is solved, and a new tool and a new method are provided for AAV activity test in neurobiology research.

The application discloses a waterproof and breathable type brain and spinal cord nerve injury wound protection dressing, which is sequentially stacked and arranged from inside to outside as a wound adhesion inner layer, a functional sustained-release middle layer and a waterproof and breathable outer layer; the three-layer synergistic dressing structure of the wound adhesion inner layer, the functional sustained-release middle layer and the waterproof and breathable outer layer is constructed, waterproof sealing and the dynamic adaptation of the air permeability performance of the brain and spinal cord nerve injury wound are realized, the target anti-scar polypeptide is covalently bonded on the inner layer, the effective inhibition of excessive scar hyperplasia and neuroma formation is realized, the matching regulation of the gradient degradation drug-loaded microsphere system and the air permeation pore size of the middle layer is realized, the accurate release of active factors in the whole cycle of nerve repair is realized, the long-acting waterproof and breathable protection in a dynamic scene is realized through the design of the outer layer of super-hydrophobic nanofiber membrane, the risk of postoperative complications is reduced, and the integration and synergy of the physical protection of the wound and the nerve function repair are realized.

The invention relates to a nerve conduit for connecting an impaired nerve ending to a target site. In particular, the present invention relates to nerve conduits that support nerve injury repair and motion control and / or reduce the risk of developing a neuroma at an impaired nerve ending. Thus, a nerve conduit (10) for connecting a nerve ending to a target site is proposed, comprising an elongated body (12) formed by a wall (22) defining an internal cavity (16), and comprising: a central portion (14) wherein the internal cavity (16) extends through the central portion (14); and two end portions (18, 19) defining respective openings (20, 21) to the lumen (16), one end portion (18) being an insertion portion configured for insertion into a respective nerve ending and the other end portion (19) being a nerve ending exit portion. According to the invention, the end portions (18, 19) are arranged such that the cross-sections of the openings (20, 21) are oriented at an angle to one another.

A composition comprising a liquid plasma according to the present invention can induce growth inhibition and death of brain tumor cells through the combined effect of radicals, ionic species, and pH effect, and thus can be effectively used for preventing or treating brain tumors. In addition, the present invention is a non-invasive physical and chemical treatment approach with low drug dependence, can exhibit a synergistic effect when used in combination with existing anticancer drugs, and has a high potential for application to the treatment of various nervous system tumors or metastatic brain diseases including glioblastoma and auditory nerve tumors.

The invention relates to the technical field of bioengineering, in particular to a culture medium and a method for constructing auditory neuroma organs. According to the culture medium, Neurobase Medium and DMEM (Dulbecco Modified Eagle Medium) / F12 are used as a basic culture medium; the feed additive comprises a N2 additive, a B27 additive, L-alanyl-L-glutamine, an antibiotic, HEPES, glucose, beta-mercaptoethanol, a cell growth factor, MEM NEAA and a TGF-beta inhibitor. The cell growth factor is EGF (Epidermal Growth Factor) or FGF-2. The culture medium provided by the invention can effectively improve the construction efficiency of the auditory neuroma organoid, and the constructed auditory neuroma organoid can be used as a reliable in-vitro substitution model, is used for marker detection, drug screening and individualized medical exploration in basic research and clinical transformation research, and has important application value.

The invention provides a nerve conduit, which comprises a cannula, one end of the cannula is provided with an opening, and the other end of the cannula is provided with a folding part; the shape of the filling block is matched with the internal shape of the sleeve; when the filling block is assembled in the sleeve, the distance between one end, close to the opening, of the filling block and one end of the opening of the sleeve is preset; wherein a plurality of through holes are formed in the side wall of the sleeve, and the through holes are configured to provide passages for permeation of tissue fluid; and the filling block is made of a degradable biomedical material. According to the nerve conduit provided by the invention, a protective envelope is formed through the sleeve, replaces an adventitia, coats and limits the growth of nerve tracts, and nerves can be subjected to the action of relative mechanical stress along with the narrowing of the surrounding growth environment, so that the formation of neuroma is more quickly and stably prevented, the extrusion of surrounding muscles in a body is resisted, and the terminal nerves are protected. The nerve conduit is simple in structure and has good mechanical properties, and industrial mass production can be achieved.

The subject invention provides devices and methods for alleviating discomfort associated with neuroma formation. The devices and methods of the invention effectively use the body's natural response of reconstructing implanted biomaterials to minimize the size of, isolate, and protect a neuroma. In preferred embodiments, the subject device is a cylindrical cap, wherein the internal chamber of the cylindrical cap physically partitions the nerve to enable an arrangement of nerve fibers (as opposed to haphazardly arranged nerve fibers often produced in neuromas). Tabs arranged on the outside of the cap can be used to manipulate the cap into place on a nerve. The open end can also be configured with flaps that can be used to widen the open end for easier insertion of the nerve into the cap. In addition, the cap's material remodels into a tissue cushion after implantation, which protects the neuroma from being stimulated and inducing pain.

The invention provides a nerve conduit, which comprises a sleeve, one end of the sleeve is provided with an opening, and the other end of the sleeve is provided with a fixing part; the supporting frame comprises one or more supporting plates; when the supporting frame is assembled in the sleeve, the interior of the sleeve is divided into a plurality of axial limiting channels by the supporting plates, and the limiting channels are configured to provide a preset growth path; a plurality of through holes are formed in the side wall of the sleeve, and the through holes are configured to provide passages for permeation of tissue fluid. According to the nerve conduit provided by the invention, a protective envelope is formed through the sleeve, replaces an adventitia, coats and limits the growth of nerve tracts, and nerves can be subjected to the action of relative mechanical stress along with the narrowing of the surrounding growth environment, so that the formation of neuroma is more quickly and stably prevented, the extrusion of surrounding muscles in a body is resisted, and the terminal nerves are protected. The nerve conduit is simple in structure and has good mechanical properties, and industrial mass production can be achieved.

The present disclosure provides a mechanically-actuated tool for cutting a tissue graft having a hollow core and methods for use thereof. A portion of a biological structure, such as a nerve, is attached to the hollow core to form an implantable neural graft assembly. The tool has a cutter mechanism and a grasper mechanism. The grasper mechanism has one or more component(s) that open and close via an actuation mechanism, like a handle, and rotate via a controller component, like a rotatable wheel. The cutter mechanism may be a cutting tube component that harvests the tissue graft. The tool may also have an ejector mechanism to remove the tissue graft as part of the implantable neural graft assembly. Such devices and methods are particularly suitable for treating neuromas and other neural regeneration procedures.

A nerve conduit for supporting repair of a nerve lesion and motor control and / or reducing risk of the development of a neuroma at the lesioned nerve end. The nerve conduit includes an elongate body formed by a wall and including a central portion defining an inner cavity and end portions defining a respective opening to the inner cavity and arranged adjacent to the central portion and at longitudinally opposing ends of the elongate body. One end portion is configured as an insertion portion configured for inserting a respective nerve end. The other end portion is configured as a nerve end outlet portion, an outer diameter of the wall defining the outlet portion is larger than an outer diameter of the wall defining the central portion.

The invention discloses a nerve end cap of polycaprolactone compounded amorphous calcium phosphate and a preparation method of the nerve end cap, and relates to the technical field of biological materials and medical treatment. The end cap is formed by pressing nanofiber after film forming, and the nanofiber is prepared from a mixed solution of polycaprolactone and amorphous calcium phosphate. The nerve end cap provided by the invention is prepared through a pressurizing process, so that the nerve end cap has good mechanical properties, the preparation process is modularized, the operation is simple, mass production is expected to be realized, and the nerve end cap has good industrial application prospects. The nerve end cap provided by the invention is novel in structure, nerves can be subjected to the action of relative mechanical stress along with the narrowing of the surrounding growth environment, so that the formation of neuroma can be more quickly and stably prevented, and the adopted materials have good biocompatibility, so that the formation of neuroma can be remarkably prevented; and the material has good compressive mechanical properties, resists extrusion of internal surrounding muscles, and protects terminal nerves.

This invention discloses an iridium complex, its preparation method, and its application in the treatment of neuromas. The iridium complex has the following structure: The iridium complex of this invention is a near-infrared fluorescent iridium complex, which exhibits a strong photodynamic therapeutic effect on the mouse neuroma cell line (Neuro-2a cells). Under light irradiation, it strongly inhibits the growth and proliferation of mouse neuroma cells (IC50). 50 The concentration was 0.05 μM, while under dark conditions, its cytotoxicity was only 87.5 μM, and the phototherapy index (PI) was as high as 1750. This is of great significance for the research of metal drugs for the treatment of neuromas.