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Recombinant oncolytic Newcastle disease virus for expressing human GM-CSF and use thereof

A GM-CSF, Newcastle disease virus technology, applied in the field of recombinant oncolytic Newcastle disease virus, can solve the problem of poor immunotherapy effect and other problems

Pending Publication Date: 2021-09-07
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a recombinant oncolytic Newcastle disease virus expressing human GM-CSF and its application, which solves the problem of ineffective immunotherapy caused by tumor immunosuppressive microenvironment in the current tumor treatment, especially in solid tumor immunotherapy. good question

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  • Recombinant oncolytic Newcastle disease virus for expressing human GM-CSF and use thereof
  • Recombinant oncolytic Newcastle disease virus for expressing human GM-CSF and use thereof
  • Recombinant oncolytic Newcastle disease virus for expressing human GM-CSF and use thereof

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Embodiment Construction

[0036] The present invention will be described in detail below with reference to the drawings and specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. The test methods for which specific conditions are not indicated in the following examples are usually operated under conventional conditions, and the steps are not described in detail because they do not involve the invention point.

[0037] When the examples give numerical ranges, it should be understood that, unless otherwise stated in the present invention, the two endpoints of each numerical range and any value between the two endpoints can be selected. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, equipment, and materials used in the embodiments, according to those skilled in the art's gra...

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Abstract

The invention relates to the field of biological virus construction and particularly discloses a recombinant oncolytic Newcastle disease virus for expressing human GM-CSF. The recombinant oncolytic Newcastle disease virus is obtained by inserting a human GM-CSF coding gene into a full-length genome plasmid of a Newcastle disease virus Italien strain and reviving the virus in vitro. The human GM-CSF gene is as shown in SEQ ID NO.1. A GM-CSF molecule is used for recruiting DC cells from mononuclear cells, activating neutrophil and promoting differentiation and maturation of the DC cells, especially has an important effect on promoting processing and presentation of antigens, combines a characteristic of selective replication of tumors of the oncolytic Newcastle disease virus, directly kills tumor cells, causes inflammation, recruits more DC cells, promotes maturation of the DC cells and presentation of tumor antigens, enhances local and whole-body anti-tumor immune responses of tumors, improves a treatment effect of single or combined use of the oncolytic virus in treating solid tumors, and brings a new treatment thought for tumor immunotherapy.

Description

technical field [0001] The invention belongs to the field of biological virus construction, in particular to a recombinant oncolytic Newcastle disease virus expressing human GM-CSF and its application. Background technique [0002] Oncolytic virus refers to a class of viruses that can specifically kill tumor cells. Using oncolytic virus to treat tumors is one of the research hotspots in tumor biotherapy. Oncolytic viruses mainly kill tumors through two mechanisms. One is the host cell dysfunction caused by virus infection or the direct death of host cells caused by massive virus replication. Second, after the oncolytic virus infects tumor cells, it enhances the local and even systemic anti-tumor immune response by enhancing the presentation of tumor antigens, causing immunogenic death of tumor cells, and releasing a variety of cytokines and chemokines, resulting in a more durable and broad antitumor effects. With the rise of tumor immunotherapy in recent years, the latter ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/85C12N15/27A61K35/768A61K38/19A61K45/06A61P35/00C12R1/93
CPCC12N7/00C12N15/85C07K14/535A61K35/768A61K38/193A61K45/06A61P35/00C12N2760/18121C12N2760/18132C12N2760/18152C12N2800/106A61K2300/00
Inventor 边惠洁陈志南尉丁刘满张仁宇孔令敏南刚
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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