A Combination Drug for Immunotherapy of Malignant Tumors
A technology for immunotherapy and malignant tumors, which is applied in the field of medicine, can solve the problems of unclear mechanism of action of traditional Chinese medicine, incomplete target protein, and increased difficulty in obtaining it, so as to improve the immune microenvironment of tumors, promote maturation and differentiation, and improve the body's immunity Effect
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[0033] Embodiment: the pharmacological test of different groups of drugs
[0034] 1. Materials and methods
[0035] 1.1 Preparation of live bacteria freeze-dried powder:
[0036] 1) Bacterial recovery: thaw the strains stored at -80°C, and inoculate them in liquid medium with an inoculation loop.
[0037] 2) Bacterial activation: After the activation of the bacteria is successful, first streak the plate, pick a single colony visible to the naked eye, and carry out expansion culture and culture to the logarithmic phase.
[0038] 3) Bacteria collection: Bacteria cultured in liquid to the logarithmic phase, centrifuged at 8000rpm at room temperature for 5-10min, discarding the supernatant.
[0039]4) Bacterial washing: Wash once with equal volume and half volume of normal saline, centrifuge at 8000rpm for 5-10min at room temperature, and discard the supernatant.
[0040] 5) Resuspension bottling: Resuspend the processed bacteria with an appropriate volume (1:4) of cryoprotecta...
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