141 results about "Leaf curl" patented technology

The invention discloses an organic yellow peach cultivating method which comprises the steps of garden building, field planting, shaping, fertilizing, crown regulation, flower and fruit management and pest control. According to the method, a large-scale cultivating method with standard management achieved easily is adopted, common diseases such as anthracnose, the bacterial shothole disease, the bleeding disease and leaf curl and diseases caused by aphids, buscks, longicorns and dichocrocis punctiferalis occurring during yellow peach cultivation are reduced, the yield and quality of yellow peaches are improved, and further processing of yellow peach products is facilitated.

The invention relates to a method for cultivating over summering tomato plants in a sunlight greenhouse. The method includes the steps that tomato plants are fixedly planted on the two sides of each concave-shaped cultivation ridge; a drip irrigation zone is arranged in the middle of each concave-shaped cultivation ridge; a white ground silver reflective film covers each concave-shaped cultivation ridge, the silver reflective face of each white ground silver reflective film faces towards the ground, and an east-west direction steel wire is fixed at the north end of a planting line; a north-south direction steel wire is drawn in the longitudinal direction of the middle of each concave-shaped cultivation ridge; the north-south direction steel wires and the concave-shaped cultivation ridges are the same in length, and the two ends of each north-south direction steel wire are fixed on two steel wires; the white ground silver reflective films cover the north-south direction steel wires, the concave-shaped cultivation ridges are fully covered, and the silver faces face towards the ground. When the mean highest temperature in the sunlight greenhouse is 32-36 DEG C, the method can be used for fixed planting so that the ground temperature of the roots of the tomato plants 5-15cm away from the ground can be stabilized below 28 DEG C, the temperature is lowered by 2.3-4.4 DEG C on average compared with a conventional planting method, according to the conventional planting method, the ground temperature of the roots 5-15cm away from the ground is above 28 DEG C, the occurrence rate of tomato plants yellowing leaf curl virus diseases is low (only 4.1%), the occurrence rate can be reduced by 55.4-67.3% compared with the conventional planting method, and the difference is extremely obvious.

The invention relates to the technical field of planting and grafting methods of garden modeling tree species, in particular to a multi-twig colorful-leaf viewing modeling bonsai tree artificial cultivation method. The method includes the steps of tree well excavating, seedling selection, field planting, planting, grafting, field management operation, shaping and tree forming and the like. A plurality of ulmus pumila stocks are planted into a container and processed as an integral whole to form a multi-twig multi-joint organic whole. Grafting is conducted through a cut grafting method, and a method of greatly shortening the cultivation period is utilized to achieve the aim of quick forming. The cultivation method greatly improves utilization and viewing value of ulmus pumila and ulmus pumila cvjinye, and the market demand is met through industrialization production. The grafting survival rate reaches 95%, the transplanting survival rate reaches 100%, and the morbidity of leaf curl is reduced to be lower than 85%. Meanwhile, the method is simple and easy to learn, easy to operate and convenient to popularize and apply.

The invention discloses protein encoded by a leaf-shaped control gene SRNL1 of rice. The protein has an amino acid sequence shown as SEQ ID No.2. The invention also discloses a gene for encoding the protein. The gene has a nucleotide sequence shown as SEQ ID No.1. The gene can be used for culturing the rice with curled leaves.

The invention belongs to the technical field of plant molecular marker technology, and particularly relates to a double-SNP marker of a gene Ty-1 related to resistance to tomato yellow leaf curl virus disease, cDNA sequences of disease-resistant gene Ty-1 and disease-infected gene (namely allele gene) ty-1 and positions thereof on a whole genome can be obtained from the reported WO2012125025 document. Through sequence comparison, the disease-infected and disease-resistant specific primers NL2 and NL3 can be designed respectively by selecting two multielement SNP-locus GT / CG and GTG / ACA, and are mixed to form a NL2-3 double-SNP marker primer. The three types of Ty-1 and ty-1 can be detected by the NL2-3SNP marker through once PCR and gel electrophoresis display.

The invention discloses a molecular marker auxiliary selection method for tomato yellow leaf curl viral disease and knot nematode. The method is characterized by comprising the following steps of: (1) extracting DNA from a tomato material F1 and a selfed separation offspring F2 individual; (2) performing multiple PCR amplification on the tomato material F1 and the selfed separation offspring F2; and (3) performing resistance evaluation on the breeding separation generation material according to the detection result. The multiple PCR amplification method can amplify Ty-1 genes and Mi genes in the same reaction system at the same time, so the method greatly simplifies experiment operation and experiment expense, is suitable for identifying and analyzing a large amount of sample material, greatly shortens the time and reduces the workload.

The invention discloses a quick, simple and effective tomato yellow leaf curl virus resistance determination method and belongs to the field of plant protection. Traditionally, the determination of the tomato yellow leaf curl virus resistance is performed by a Bemisia tabaci (Gennadius) inoculation determination method, while the Bemisia tabaci (Gennadius) inoculation determination method has difficulty in providing uniform and consistent inoculation pressure due to the influences (such as the number of the inoculated Bemisia tabaci (Gennadius), virus rate and feeding habit) of the Bemisia tabaci (Gennadius) and at the same time, suffers interference from other Bemisia tabaci (Gennadius) transmitted viruses, so the accuracy of the determination result is low. The agrobacterium rhizogenes injection inoculation method well solves the problem. The resistance of a tomato material to the tomato yellow leaf curl viruses can be determined by using constructed infectious clones and directly inoculating the tomato yellow leaf curl viruses by injection in stalks. The method avoids culturing Bemisia tabaci (Gennadius), ensures simple operation, high efficiency, high repeatability and high controllability, provides a relatively consistent pressure, obtains accurate result and is a simple and effective inoculation determination method.

The invention relates to a plant-virus resisting composition which contains active constituents. The plant-virus resisting composition is characterized in that the active constituents contain amino-oligosaccharins and ribavirin; the weight ratio of the amino-oligosaccharins to the ribavirin is 1: (0.03-20). The invention also relates to a plant-virus resisting medicament prepared from the plant-virus resisting composition. The dosage form of the plant-virus resisting medicament can be soluble liquid, soluble powder, water dispersible granules, granules or water aqua. The invention also relates to an application of the plant-virus resisting medicament in prevention and treatment of one or multiple plant viruses comprising tobacco mosaic viruses, cucumber mosaic viruses, potato Y viruses, tomato mosaic viruses, tomato yellow leaf curl viruses and tomato spotted wilf viruses. The plant-virus resisting composition and the plant-virus resisting medicament are good in prevention and treatment effects, and therefore, the medicament using amount can be reduced, the cost is reduced, and the pollution to the environment is relieved; the plant-virus resisting medicament does not only contain a single active constituent, so that the plant viruses difficultly produce drug resistance; the plant-virus resisting medicament can prevent and treat various different types of plant-virus diseases.

The invention relates to the technical field of planting and grafting methods of garden modeling tree species, in particular to an artificial cultivation method for a multi-head colorful-leaf viewing modeling bonsai tree. The method includes the steps of tree well excavating, seedling selection, field planting, planting, grafting, field management operation, shaping and tree forming and the like. The method utilizes different planting technologies and grafting modes in an integration mode, and fully utilizes the characteristic that ulmus pumila stock crowns have many framework joints and are high in crown forming. Grafting is conducted through a cut grafting method, and a method of greatly shortening the cultivation period is utilized to achieve the aim of quick forming. The cultivation method greatly improves utilization and viewing value of ulmus pumila and ulmus pumila cvjinye, and market demand is met through industrialization production. The grafting survival rate reaches 90%-95%, the transplanting survival rate reaches 100%, and the morbidity of leaf curl is reduced to be lower than 85%. Meanwhile, the method is simple and easy to learn and easy to operate, saves resources, and is low in cost and convenient to popularize and apply.

The invention discloses a processing method of mulberry leaf black tea. The processing method comprises the following steps of withering fresh mulberry leaves and fresh tea leaves so as to obtain withered mulberry leaves and withered tea leaves; uniformly mixing the withered mulberry leaves with the withered tea leaves in the proportion of the withered mulberry leaves to the withered tea leaves being (5-10) to 1, and performing twisting until the leaves are bent to form strips; and placing the twisted leaves under the condition that the temperature is 25-30 DEG C, and the humidity is greater than or equal to 90%, and performing fermentation for 2-4h; baking the fermented leaves, performing drying, and performing spreading for cooling to room temperature so as to obtain the mulberry leaf black tea. The processing method disclosed by the invention has the advantages that an appropriate quantity of tea leaves are added to the mulberry leaves, so that the concentration of substrates and polyphenol oxidase are increased, fermentation can be guaranteed to be performed sufficiently, and green odor and green and astringent taste of the mulberry leaves are removed; the tea leaves are added, so that a certain quantity of flavored substances and fragrance substances are added, the organoleptic quality of the mulberry leaf black tea is greatly improved, and besides, the nutrient component and the health-care efficacy of the mulberry leaf black tea are also improved. The technology is simple to operate, the production cost is low, and the product quality is good.

The invention discloses an application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance and an application of the phytosulfokine-alpha in preparing preparations capable of improving plant yellow leaf curl virus resistance. According to the preparations prepared by the active ingredient, namely the phytosulfokine-alpha, signal paths of auxin in plants are induced to improve the resistance to the yellow leaf curl virus remarkably, so that economic loss caused by the yellow leaf curl virus to plants is decreased. The way of applying the preparations to prevent the plant yellow leaf curl virus is simple and easy and low in cost, capable of delaying and suppressing growth of the plant yellow leaf curl virus on leaves and spreading of diseases remarkably and improving the plant yellow leaf curl virus resistance greatly.

The invention provides a grafting cultivation method of flowering peaches. The grafting cultivation method comprises the following steps of (1) seedling cultivation land arrangement, wherein a non-continuous-cropping neutral-sandiness land parcel free of sunshade objects is selected as seedling cultivation land, the seedling cultivation land is tilled after base fertilizer is applied to make a seedling cultivation bed, soil of the seedling cultivation land is treated through pesticide, and leveling and watering are carried out; (2) stock selection and treatment, wherein mountain peaches are selected as stocks, mountain peach pits are soaked in clear water for 12 days in November, floating mountain peach pits are removed, the rest of the mountain peach pits are taken out to be aired, and sown in the seedling cultivation bed in line, the mountain peach pits are placed laterally in the line sowing process, and the mountain peach pits are covered with soil after line sowing; (3) stock seedling management; (4) grafting; wherein branches on flowering peach seed trees growing normally are selected as cions, the branches are sheared to be 7 cm long, T-shaped bud grafting is carried out on stock seedlings and the cions in July, plastic cloth filled with wet soil is tied at the positions 10 cm lower than connectors, buds on the cions are exposed, and the upper portions of the cions are sleeved with plastic bags; (5) management carried out after grafting; (6) leaf-curl prevention and treatment. The grafting survival rate and nursery stock quality are high.

The invention relates to a duplex PCR (polymerase chain reaction) primer and a method for identifying tomato chlorosis viruses and tomato yellow leaf curl viruses. The method comprises the following steps that (1) plant total RNA (ribose nucleic acid) is extracted; (2) the plant total RNA is used as a template for synthesizing cDNA (complementary deoxyribonucleic acid) through reverse transcription; (3) the cDNA is used as a template for performing PCR amplification on a nucleotide sequence coded by ToCV and TYLCV capsid protein; (4) a PCR product prepared in the step (3) is subjected to agarose gel electrophoresis analysis. The invention relates to a PCR primer combination. A duplex PCR method is used for performing ToCV and TYLCV identification; the result is accurate; the method is fast, simple and convenient; the detection cost is reduced; the virus detection efficiency is greatly improved; good application prospects are realized.

The invention discloses a molecular marker method for identifying a tomato yellow leaf curl virus resistance gene Ty-1. The method comprises the following steps: extracting a tomato gene group DNA; performing PCR amplification on the extracted tomato gene group DNA by using a molecular marker primer and performing electrophoretic analysis on the obtained PCR amplification product, representing that the yellow leaf curl virus resistance gene Ty-1 in a plant is a disease-resistant plant if a stripe with the length of 690bp is generated in an electrophoretogram, and representing that the plant is a plant system containing ty-1 gene site and is not a disease-resistant plant if the stripe with the length of 409bp is generated in the electrophoretogram. The electrophoretogram in the marker method is simple in stripe form, easy to identify, can be directly applied to marker assistant selection and can improve the identification accuracy; and moreover, the time is saving, and the material resources and the labor force are reduced.

The invention discloses fertilizer for resisting peach tree leaf curl disease. The fertilizer is prepared from the following raw materials in parts by weight: 8 to 10 parts of urea, 7 to 8 parts of potassium chloride, 6 to 8 parts of ammonium phosphate, 10 to 15 parts of potassium humate, 1 to 2 parts of calcium humate, 3 to 4 parts of ferric humate, 2 to 3 parts of magnesium amino acid, 0.5 to 1 part of trace elements, 7 to 9 parts of traditional Chinese medicinal extracts, 0.5 to 1 part of modifier, 3 to 4 parts of additive and 25 to 30 parts of water. According to the fertilizer disclosed by the invention, by matching various substances, germs are killed and the disease is controlled from the outside, and the own disease resistance of peach trees is activated and strengthened from the inside while proper nutrients are provided; in addition, the traditional Chinese medicinal extracts are promoted to enter the inside of the peach trees and are matched with the modifier, so that the disease resistance of the peach trees is greatly improved, the germs are killed, and further the peach tree leaf curl disease is thoroughly treated from inside to outside.

The invention discloses a method for detecting sweet potato leaf curl viruses and a special primer set thereof. The primer set is composed of single-stranded DNA molecules shown by a first sequence, single-stranded DNA molecules shown by a second sequence, single-stranded DNA molecules shown by a third sequence, single-stranded DNA molecules shown by a fourth sequence, single-stranded DNA molecules shown by a fifth sequence and single-stranded DNA molecules shown by a sixth sequence. The primer set has the functions of authenticating or assisting to authenticate the sweet potato leaf curl viruses and detecting whether the sweet potato leaf curl viruses are contained in a to-be-detected biological sample or not. The primer set for detecting the sweet potato leaf curl viruses has the advantages that specificity is high, sensitivity is high, operation is easy and convenient, reaction time is obviously shortened, and the defect that an existing method needs to rely on expensive instruments is overcome. The method and the primer set have the high popularization value.

The invention discloses a compound microbial agent and application thereof in preventing and treating various plant diseases. The compound microbial agent has a remarkable inhibition effect on variousmain soil-borne diseases such as bacterial wilt, root rot, soft rot, black shank, gibberellic disease and root black rot caused by bacteria, fungi and oomycetes; meanwhile, the biomass of solanaceaecrop tomatoes, brassicaceous crop brassica campestris and cucurbitaceae crop cucumbers is remarkably improved, and a remarkable inhibiting effect is also achieved on tomato yellow leaf curl viruses which are plant virus diseases. The compound microbial agent effectively solves the problems of environmental pollution and food quality caused by the fact that the conventional microbial agent only aims at single pathogenic bacteria and chemical pesticide prevention and treatment, and has a good application prospect in green and environment-friendly and agricultural sustainable development.

The invention belongs to the field of biotechnology and botany, and particularly provides a construction method of a rolling-circle replication recombinant vector for heterologous protein expression,the recombinant vector and an application of the recombinant vector. According to the construction method provided by the invention, the high-efficiency rolling-circle replication transient expressionvector mainly based on the sequence of a sweet potato leaf curl virus (SPLCV) is constructed, so that duration time and expression abundance of heterologous nucleic acids and proteins on plants are remarkably improved, and the results of the vector have high repeatability and reliability. The recombinant vector provided by the invention has a molecular tool vector which has an extremely long expression time to heterologous proteins (longer than 20 day compared with the expression time of a common transient expression T vector) and has an extremely high expression abundance (larger than 30-fold expression compared with a common transient expression T vector), and the recombinant vector endows different plants (tobaccos, sweet potatoes and morning glory) with heterologous protein expressioncapability, and makes up the blank of related plants for utilizing transient expression to study gene functions.

The invention discloses an RPA detection primer for a sweet potato leaf roll virus, a detection method and an application. The RPA detection primer is specially used for specific molecular detection of the sweet potato leaf roll virus. The detection primer is as follows: an upstream primer F is as shown in SEQ ID No: 1; and a downstream primer R is as shown in SEQ ID No: 2. The detection method comprises the following steps: extracting plant DNA infected with the sweet potato leaf roll virus, carrying out RPA isothermal amplification for 20 min, and after completion of amplification, carryingout agarose gel electrophoresis so as to generate a specific band. The RPA isothermal amplification technology established for the sweet potato leaf curl virus provided by the invention has the advantages of rapid and highly-efficient amplification, good specificity, easy and convenient operation, no need of special instruments and the like, and provides a technical basis for early diagnosis of the sweet potato leaf curl virus.

The invention discloses a resistance identification method of sweet potato leaf curl virus disease. The method includes following steps: obtaining original seedlings not containing leaf curl virus disease SPLCV after a to-be-identified variety is subjected to culture of virus-free test tube seedlings and molecular detection; putting the original seedlings into hole trays for quick propagation; allowing high-power expanding propagation of leaf curl virus indicating plants; identifying insect-borne virus inoculation when variety virus-free tissue culture seedlings are isolated and in an airtightcondition; identifying grafting virus inoculation in a plant net room condition; performing yield comparison experiment planting, harvesting, measuring yield, and conserving seeds; discharging seedsin the next year, and investigating and identifying emerging seedling leaf curl virus symptom showing rate; evaluating leaf curl virus resistant (tolerant) characteristics of the variety. Propagationlaws of the SPLCV are utilized, natural virus transmission, insect-borne virus inoculation and grafting virus inoculation are performed on the identified variety at different stages, grading is performed according to grading standards through seedling bed performance of each variety in the next year, resistant, tolerant and sensitive characteristics of the variety to the SPLCV are determined, anda basic resistant source is provided for breeding of SPLCV resistant varieties.

The invention discloses a method for breeding a novel tomato line rich in lycopene and resisting against a yellow leaf curl virus. The method comprises the following steps: screening a recessive mutant gene dg specificity Caps molecular marker according to tomato genome DNA (Deoxyribonucleic Acid) sequence signatures; screening a pair of Caps molecular markers which are closely linked with the anti-TYLCV (Tomato Yellow Leaf Curl Virus) gene Ty-3; hybridizing a tomato material containing the TYLCV resistance gene and the tomato mutant dg material with a common tomato good selfing line; conducting multi-generation selfing and taking the dg and Ty-3 molecular markers as basis to screen single plants; at last, obtaining the excellent selfing line aggregating the dg gene and the Ty-3 gene. The method can also be applied to a hybrid which is obtained by utilizing the selfing line to be hybridized with the other tomato selfing lines.

The invention discloses an SSR (simple sequence repeat) marker for tomato yellow leaf curl disease resisting character co-segregation and application thereof. The SSR marker includes primers: upstream primer T5-F: 5'-CAATCAATCGATGCACAAAACACC-3'; downstream primer T5-R: 5'-GACTGACTGCATTGGATTTGGCTT-3'. The marker can be amplified in CLN32120a-23 into a 580bp strip, can be amplified in Money marker into a 640bp strip and can be amplified in F1 into the above two strips, marking results are substantially consistent to that of field identification, it is proved that the marker can distinguish anti-disease materials, disease-sensitive materials and hybrid anti-disease materials and is a co-dominant marker in tight linkage with tomato yellow leaf curl disease virus resisting gene ty-5. Molecular marker detection is 90% matching with field detection in terms of detection results. The marker can be used in PCR (polymerase chain reaction) quick detection for tomato yellow leaf curl virus resisting ty-5 gene, and certain theoretical and practical basis is provided for the application of ty-5 molecular marker assisted selective breeding.

The invention discloses a bambusa multiplex transcription factor BmMYB83 gene and application thereof and belongs to the technical field of plant gene engineering. The transcription factor BmMYB83 disclosed by the invention belongs to an MYB transcription factor family, is derived from bambusa multiplex and has a nucleotide sequence shown in SEQ ID NO.1, and the amino acid sequence of a protein encoded by the gene is shown in SEQ ID NO.2. Through gene cloning expression and comparison analysis, the evolutionary status of an encoding gene system of the bambusa multiplex transcription factor BmMYB83 is revealed, a method for using the gene is also provided, plant type variation, such as planting dwarfing, leaf curling and number increase of scapes, of arabidopsis plants caused through over expression of the gene, and root lengths of rice can be also reduced. The gene disclosed by the invention plays a significant role in plant property improvement.

The invention discloses a Phyllostachys edulis PeTCP10 protein capable of controlling leaf curling, and applications thereof, wherein the amino acid sequence of the Phyllostachys edulis PeTCP10 protein is represented by SEQ ID No.3. The invention further provides a gene sequence encoding the Phyllostachys edulis PeTCP10 protein, wherein the deoxynucleotide sequence of the Phyllostachys edulis PeTCP10 protein is represented by SEQ ID No.2. The invention further relates to a vector containing the deoxynucleotide sequence of the gene. According to the present invention, a recombinant overexpression vector pCAMBIA1301-35S-PeTCP10 is transfected into wild-type Arabidopsis thaliana by using an Agrobacterium-mediated inflorescence impregnation method, and the results show that the leaf curling degree of the transgenic plant over-expressing PeTCP10 is significantly higher than the leaf curling degree of the wild type plant, such that the transgenic Arabidopsis thaliana can produce significantleaf curling through the Phyllostachys edulis PeTCP10 gene.

The invention belongs to the field of plant gene engineering, particularly relates to a rice half-leaf-curl gene SRL10 cloned through a map-based cloning technology and determining of the gene function through a transgenic complementation experiment, and meanwhile further relates to gene editing of the gene. The leaf morphology of a plant is improved, the ideal plant type of the rice can be formed, and the yield of crops is improved. The invention discloses a protein for encoding the rice leaf form control gene SRL10. The protein has the amino acid sequence shown in SEQ ID No:2. The inventionfurther meanwhile discloses a gene for encoding the protein. The gene has the nucleotide sequence shown in the SEQ ID No:1.

The invention discloses a preparation technology of high-quality folium llicis latifoliae. The method comprises the following steps: naturally withering fresh leaves and enabling the tea leaves to bedehydrated and soft; carrying out manual fixation until the appearance leaf color becomes dark green from clear green, the tea leaves are slightly sticky when being held in hands and thick stems cannot be broken, wherein the temperature of a pot is 150 DEG C to 200 DEG C, is high at first and then is reduced; in a rolling process, rolling while frying by utilizing slow fire; when the adhesive force of the tea leave is great, twisting the tea leaves into blocks; taking the blocks out from the pot and molding, wherein the molding rate of tender leaves is 80 percent or more and the molding rate of over-aged buds is 60 percent to 70 percent; after withering the fresh tea leaves, carrying out fixation, rolling and shaping; immediately airing to obtain the tea leaves with the moisture content of80 percent or more. The folium llicis latifoliae processed by the preparation technology has a star-shaped or curled shape; the tea leaves have uniform thickness and the tea leaves are oily and smooth, are not scorched and crushed and have enough aroma; the tea soup is yellow, green and bright and has a mellow and astringent taste; after people drink the tea soup, the taste is bitter and then issweet and the aftertaste is sweet and lasting.

The invention discloses an OsRoc8 gene of a plant leaf curl related protein and the protein, and application of an expression kit, an recombinant vector or cell in obtaining a plant with curled leaves. According to the present invention, the OsRoc8 gene is homologously cloned from rice to a transcription, the complete cDNA of the OsRoc8 gene is homologously recombined into a plant vector with overexpression, the plant is transformed by an Agrobacterium infection method, the corresponding transgenic plant is relative upright with leaves curled to the front, the photosynthetic efficiency increases, and then transgenic plants with high photosythetic efficiency and curled leaves can be cultivated.

The invention relates to a method for environment-friendly prevention and control on bemisia tabaci and TYLCV of tomatoes at a seedling stage. The method comprises the steps: (1) sowing tomatoes in culture dishes completely enclosed by gauze cages; (2) applying fluoropyrazole furanone 200SC and spirotetramat 240SC to cultured tomato seedlings in an irrigating manner; (3) transplanting the tomato seedlings obtained in the step (2) into greenhouse films; (4) broadcasting salicylic acid slow-releasing granules; (5) spraying 6% of oligosaccharide chain protein wettable powder to the tomato seedlings obtained in the above steps; and (6) separately spraying the fluoropyrazole furanone 200SC and the spirotetramat 240SC to the tomato seedlings obtained in the above steps. According to the method for environment-friendly prevention and control on the bemisia tabaci and TYLCV of the tomatoes at the seedling stage, a variety of novel, efficient and low-toxicity pesticides and plant immunity activators are applied, virus-propagating key behaviors of the bemisia tabaci are blocked from vitro of plants, defense reactions of the plants are activated to resist virus propagation of the bemisia tabaci, and the bemisia tabaci and tomato yellowing leaf curl viral disease propagated thereby of the tomatoes are prevented and controlled environmentally friendly.

The invention discloses a molecular marker primer of a disease-resistant gene ty-5 of the tomato yellow leaf curl disease and application of the molecular marker primer. Sequences of the molecular marker primer are TAACAAAGCCCTCAAAGC from 5' terminal to 3' terminal of a forward primer and GTCTCCGAAACGTAATCC from 5' terminal to 3' terminal of a reverse primer. A molecular marker is of an InDel marker type, a nucleotide sequence of the molecular marker is SEQ ID No.3 or SEQ ID No.4. The InDel molecular marker linked with the gene is developed by a tomato material AVTO 1227 containing the gene ty-5 and is used for molecular marker-assisted selection. The molecular marker which is the InDel marker can be used for pleomorphic analysis without being subjected to enzyme digestion, and accordingly, convenience and simplicity in operation and low experiment cost are achieved.