141 results about "Leaf curl" patented technology

The invention relates to a method for cultivating over summering tomato plants in a sunlight greenhouse. The method includes the steps that tomato plants are fixedly planted on the two sides of each concave-shaped cultivation ridge; a drip irrigation zone is arranged in the middle of each concave-shaped cultivation ridge; a white ground silver reflective film covers each concave-shaped cultivation ridge, the silver reflective face of each white ground silver reflective film faces towards the ground, and an east-west direction steel wire is fixed at the north end of a planting line; a north-south direction steel wire is drawn in the longitudinal direction of the middle of each concave-shaped cultivation ridge; the north-south direction steel wires and the concave-shaped cultivation ridges are the same in length, and the two ends of each north-south direction steel wire are fixed on two steel wires; the white ground silver reflective films cover the north-south direction steel wires, the concave-shaped cultivation ridges are fully covered, and the silver faces face towards the ground. When the mean highest temperature in the sunlight greenhouse is 32-36 DEG C, the method can be used for fixed planting so that the ground temperature of the roots of the tomato plants 5-15cm away from the ground can be stabilized below 28 DEG C, the temperature is lowered by 2.3-4.4 DEG C on average compared with a conventional planting method, according to the conventional planting method, the ground temperature of the roots 5-15cm away from the ground is above 28 DEG C, the occurrence rate of tomato plants yellowing leaf curl virus diseases is low (only 4.1%), the occurrence rate can be reduced by 55.4-67.3% compared with the conventional planting method, and the difference is extremely obvious.

The invention relates to the technical field of planting and grafting methods of garden modeling tree species, in particular to a multi-twig colorful-leaf viewing modeling bonsai tree artificial cultivation method. The method includes the steps of tree well excavating, seedling selection, field planting, planting, grafting, field management operation, shaping and tree forming and the like. A plurality of ulmus pumila stocks are planted into a container and processed as an integral whole to form a multi-twig multi-joint organic whole. Grafting is conducted through a cut grafting method, and a method of greatly shortening the cultivation period is utilized to achieve the aim of quick forming. The cultivation method greatly improves utilization and viewing value of ulmus pumila and ulmus pumila cvjinye, and the market demand is met through industrialization production. The grafting survival rate reaches 95%, the transplanting survival rate reaches 100%, and the morbidity of leaf curl is reduced to be lower than 85%. Meanwhile, the method is simple and easy to learn, easy to operate and convenient to popularize and apply.

The invention discloses protein encoded by a leaf-shaped control gene SRNL1 of rice. The protein has an amino acid sequence shown as SEQ ID No.2. The invention also discloses a gene for encoding the protein. The gene has a nucleotide sequence shown as SEQ ID No.1. The gene can be used for culturing the rice with curled leaves.

The invention belongs to the technical field of plant molecular marker technology, and particularly relates to a double-SNP marker of a gene Ty-1 related to resistance to tomato yellow leaf curl virus disease, cDNA sequences of disease-resistant gene Ty-1 and disease-infected gene (namely allele gene) ty-1 and positions thereof on a whole genome can be obtained from the reported WO2012125025 document. Through sequence comparison, the disease-infected and disease-resistant specific primers NL2 and NL3 can be designed respectively by selecting two multielement SNP-locus GT/CG and GTG/ACA, and are mixed to form a NL2-3 double-SNP marker primer. The three types of Ty-1 and ty-1 can be detected by the NL2-3SNP marker through once PCR and gel electrophoresis display.

The invention discloses a dot enzyme-linked immunosorbent assay (ELISA) method for detecting bemisia tabaci carrying a tomato yellow leaf curl virus and application of the method. Field bemisia tabaci is collected, pretreated and grinded, monoclonal antibody of the tomato yellow leaf curl virus is used, the dot-ELISA method which is used for detecting virus transmission medium bemisia tabaci is established, a quick kit is developed and researched, and the tomato yellow leaf curl virus which is carried by the bemisia tabaci is detected. By the aid of the method, the virus carrying rate of the field bemisia tabaci can be surveyed in a large scale, and the field tomato yellow leaf curl disease outbreak trend is predicted. The method is quick, efficient, sensitive, special, high in flux and simple to operate. The tomato yellow leaf curl disease can be diagnosed quickly, predicted and controlled scientifically.

The invention discloses a molecular marker auxiliary selection method for tomato yellow leaf curl viral disease and knot nematode. The method is characterized by comprising the following steps of: (1) extracting DNA from a tomato material F1 and a selfed separation offspring F2 individual; (2) performing multiple PCR amplification on the tomato material F1 and the selfed separation offspring F2; and (3) performing resistance evaluation on the breeding separation generation material according to the detection result. The multiple PCR amplification method can amplify Ty-1 genes and Mi genes in the same reaction system at the same time, so the method greatly simplifies experiment operation and experiment expense, is suitable for identifying and analyzing a large amount of sample material, greatly shortens the time and reduces the workload.

The invention discloses a quick, simple and effective tomato yellow leaf curl virus resistance determination method and belongs to the field of plant protection. Traditionally, the determination of the tomato yellow leaf curl virus resistance is performed by a Bemisia tabaci (Gennadius) inoculation determination method, while the Bemisia tabaci (Gennadius) inoculation determination method has difficulty in providing uniform and consistent inoculation pressure due to the influences (such as the number of the inoculated Bemisia tabaci (Gennadius), virus rate and feeding habit) of the Bemisia tabaci (Gennadius) and at the same time, suffers interference from other Bemisia tabaci (Gennadius) transmitted viruses, so the accuracy of the determination result is low. The agrobacterium rhizogenes injection inoculation method well solves the problem. The resistance of a tomato material to the tomato yellow leaf curl viruses can be determined by using constructed infectious clones and directly inoculating the tomato yellow leaf curl viruses by injection in stalks. The method avoids culturing Bemisia tabaci (Gennadius), ensures simple operation, high efficiency, high repeatability and high controllability, provides a relatively consistent pressure, obtains accurate result and is a simple and effective inoculation determination method.

The invention discloses an application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance and an application of the phytosulfokine-alpha in preparing preparations capable of improving plant yellow leaf curl virus resistance. According to the preparations prepared by the active ingredient, namely the phytosulfokine-alpha, signal paths of auxin in plants are induced to improve the resistance to the yellow leaf curl virus remarkably, so that economic loss caused by the yellow leaf curl virus to plants is decreased. The way of applying the preparations to prevent the plant yellow leaf curl virus is simple and easy and low in cost, capable of delaying and suppressing growth of the plant yellow leaf curl virus on leaves and spreading of diseases remarkably and improving the plant yellow leaf curl virus resistance greatly.

The invention provides a grafting cultivation method of flowering peaches. The grafting cultivation method comprises the following steps of (1) seedling cultivation land arrangement, wherein a non-continuous-cropping neutral-sandiness land parcel free of sunshade objects is selected as seedling cultivation land, the seedling cultivation land is tilled after base fertilizer is applied to make a seedling cultivation bed, soil of the seedling cultivation land is treated through pesticide, and leveling and watering are carried out; (2) stock selection and treatment, wherein mountain peaches are selected as stocks, mountain peach pits are soaked in clear water for 12 days in November, floating mountain peach pits are removed, the rest of the mountain peach pits are taken out to be aired, and sown in the seedling cultivation bed in line, the mountain peach pits are placed laterally in the line sowing process, and the mountain peach pits are covered with soil after line sowing; (3) stock seedling management; (4) grafting; wherein branches on flowering peach seed trees growing normally are selected as cions, the branches are sheared to be 7 cm long, T-shaped bud grafting is carried out on stock seedlings and the cions in July, plastic cloth filled with wet soil is tied at the positions 10 cm lower than connectors, buds on the cions are exposed, and the upper portions of the cions are sleeved with plastic bags; (5) management carried out after grafting; (6) leaf-curl prevention and treatment. The grafting survival rate and nursery stock quality are high.

The invention discloses a compound microbial agent and application thereof in preventing and treating various plant diseases. The compound microbial agent has a remarkable inhibition effect on variousmain soil-borne diseases such as bacterial wilt, root rot, soft rot, black shank, gibberellic disease and root black rot caused by bacteria, fungi and oomycetes; meanwhile, the biomass of solanaceaecrop tomatoes, brassicaceous crop brassica campestris and cucurbitaceae crop cucumbers is remarkably improved, and a remarkable inhibiting effect is also achieved on tomato yellow leaf curl viruses which are plant virus diseases. The compound microbial agent effectively solves the problems of environmental pollution and food quality caused by the fact that the conventional microbial agent only aims at single pathogenic bacteria and chemical pesticide prevention and treatment, and has a good application prospect in green and environment-friendly and agricultural sustainable development.

The invention discloses an RPA detection primer for a sweet potato leaf roll virus, a detection method and an application. The RPA detection primer is specially used for specific molecular detection of the sweet potato leaf roll virus. The detection primer is as follows: an upstream primer F is as shown in SEQ ID No: 1; and a downstream primer R is as shown in SEQ ID No: 2. The detection method comprises the following steps: extracting plant DNA infected with the sweet potato leaf roll virus, carrying out RPA isothermal amplification for 20 min, and after completion of amplification, carryingout agarose gel electrophoresis so as to generate a specific band. The RPA isothermal amplification technology established for the sweet potato leaf curl virus provided by the invention has the advantages of rapid and highly-efficient amplification, good specificity, easy and convenient operation, no need of special instruments and the like, and provides a technical basis for early diagnosis of the sweet potato leaf curl virus.

The invention discloses a resistance identification method of sweet potato leaf curl virus disease. The method includes following steps: obtaining original seedlings not containing leaf curl virus disease SPLCV after a to-be-identified variety is subjected to culture of virus-free test tube seedlings and molecular detection; putting the original seedlings into hole trays for quick propagation; allowing high-power expanding propagation of leaf curl virus indicating plants; identifying insect-borne virus inoculation when variety virus-free tissue culture seedlings are isolated and in an airtightcondition; identifying grafting virus inoculation in a plant net room condition; performing yield comparison experiment planting, harvesting, measuring yield, and conserving seeds; discharging seedsin the next year, and investigating and identifying emerging seedling leaf curl virus symptom showing rate; evaluating leaf curl virus resistant (tolerant) characteristics of the variety. Propagationlaws of the SPLCV are utilized, natural virus transmission, insect-borne virus inoculation and grafting virus inoculation are performed on the identified variety at different stages, grading is performed according to grading standards through seedling bed performance of each variety in the next year, resistant, tolerant and sensitive characteristics of the variety to the SPLCV are determined, anda basic resistant source is provided for breeding of SPLCV resistant varieties.

The invention discloses an SSR (simple sequence repeat) marker for tomato yellow leaf curl disease resisting character co-segregation and application thereof. The SSR marker includes primers: upstream primer T5-F: 5'-CAATCAATCGATGCACAAAACACC-3'; downstream primer T5-R: 5'-GACTGACTGCATTGGATTTGGCTT-3'. The marker can be amplified in CLN32120a-23 into a 580bp strip, can be amplified in Money marker into a 640bp strip and can be amplified in F1 into the above two strips, marking results are substantially consistent to that of field identification, it is proved that the marker can distinguish anti-disease materials, disease-sensitive materials and hybrid anti-disease materials and is a co-dominant marker in tight linkage with tomato yellow leaf curl disease virus resisting gene ty-5. Molecular marker detection is 90% matching with field detection in terms of detection results. The marker can be used in PCR (polymerase chain reaction) quick detection for tomato yellow leaf curl virus resisting ty-5 gene, and certain theoretical and practical basis is provided for the application of ty-5 molecular marker assisted selective breeding.

The invention discloses a bambusa multiplex transcription factor BmMYB83 gene and application thereof and belongs to the technical field of plant gene engineering. The transcription factor BmMYB83 disclosed by the invention belongs to an MYB transcription factor family, is derived from bambusa multiplex and has a nucleotide sequence shown in SEQ ID NO.1, and the amino acid sequence of a protein encoded by the gene is shown in SEQ ID NO.2. Through gene cloning expression and comparison analysis, the evolutionary status of an encoding gene system of the bambusa multiplex transcription factor BmMYB83 is revealed, a method for using the gene is also provided, plant type variation, such as planting dwarfing, leaf curling and number increase of scapes, of arabidopsis plants caused through over expression of the gene, and root lengths of rice can be also reduced. The gene disclosed by the invention plays a significant role in plant property improvement.

The invention discloses a Phyllostachys edulis PeTCP10 protein capable of controlling leaf curling, and applications thereof, wherein the amino acid sequence of the Phyllostachys edulis PeTCP10 protein is represented by SEQ ID No.3. The invention further provides a gene sequence encoding the Phyllostachys edulis PeTCP10 protein, wherein the deoxynucleotide sequence of the Phyllostachys edulis PeTCP10 protein is represented by SEQ ID No.2. The invention further relates to a vector containing the deoxynucleotide sequence of the gene. According to the present invention, a recombinant overexpression vector pCAMBIA1301-35S-PeTCP10 is transfected into wild-type Arabidopsis thaliana by using an Agrobacterium-mediated inflorescence impregnation method, and the results show that the leaf curling degree of the transgenic plant over-expressing PeTCP10 is significantly higher than the leaf curling degree of the wild type plant, such that the transgenic Arabidopsis thaliana can produce significantleaf curling through the Phyllostachys edulis PeTCP10 gene.

The invention belongs to the field of plant gene engineering, particularly relates to a rice half-leaf-curl gene SRL10 cloned through a map-based cloning technology and determining of the gene function through a transgenic complementation experiment, and meanwhile further relates to gene editing of the gene. The leaf morphology of a plant is improved, the ideal plant type of the rice can be formed, and the yield of crops is improved. The invention discloses a protein for encoding the rice leaf form control gene SRL10. The protein has the amino acid sequence shown in SEQ ID No:2. The inventionfurther meanwhile discloses a gene for encoding the protein. The gene has the nucleotide sequence shown in the SEQ ID No:1.

The invention discloses an OsRoc8 gene of a plant leaf curl related protein and the protein, and application of an expression kit, an recombinant vector or cell in obtaining a plant with curled leaves. According to the present invention, the OsRoc8 gene is homologously cloned from rice to a transcription, the complete cDNA of the OsRoc8 gene is homologously recombined into a plant vector with overexpression, the plant is transformed by an Agrobacterium infection method, the corresponding transgenic plant is relative upright with leaves curled to the front, the photosynthetic efficiency increases, and then transgenic plants with high photosythetic efficiency and curled leaves can be cultivated.